Abstract:
:We have proposed that the transmembrane receptor encoded by the c-Kit protooncogene and its ligand play an important role in regulating the proliferation of blasts cells in acute myeloblastic leukemia (AML). To test this hypothesis, immunobeads were used to separate blasts from three Kit-expression positive cell lines into strongly Kit-protein positive and weakly Kit-protein positive fractions. The strongly positive fraction had greater proliferative potential than the weakly positive fraction as assessed both by colony-formation in methylcellulose and growth of clonogenic cells in suspension. The reproducibility of the percentage of each blast population found in the strongly and weakly positive fractions provided evidence that Kit-protein expression is regulated. Kinetic experiments provided evidence for reversible transitions between strong and weak Kit protein expression. Thus regulated expression of the Kit receptor may be a mechanism for controlling blast cell growth in culture.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Tohda S,Yang GS,Ashman LK,McCulloch EA,Minden MDdoi
10.1002/jcp.1041540225subject
Has Abstractpub_date
1993-02-01 00:00:00pages
410-8issue
2eissn
0021-9541issn
1097-4652journal_volume
154pub_type
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