Abstract：
:Glioblastoma multiforme is the most common and aggressive primary brain cancer with only ∼3% of patients surviving more than 3 years from diagnosis. Several mechanisms are involved in drug and radiation resistance to anticancer treatments and among them one of the most important factors is the tumor microenvironment status, characterized by cancer cell hypersecretion of interleukins and cytokines. The aim of our research was the synthesis of a nanocarrier of quercetin combined with temozolomide, to enhance the specificity and efficacy of this anticancer drug commonly used in glioblastoma treatment. The nanohydrogel increased the internalization and cytotoxicity of quercetin in human glioblastoma cells and, when co-delivered with temozolomide, contribute to an improved anticancer effect. The nanohydrogel loaded with quercetin had the ability to recognize CD44 receptor, a brain cancer cell marker, through an energy and caveolae dependent mechanism of internalization. Moreover, nanohydrogel of quercetin was able to reduce significantly IL-8, IL-6, and VEGF production in pro-inflammatory conditions with interesting implications on the mechanism of glioblastoma cells drug resistance. In summary, novel CD44 targeted polymeric based nanocarriers appear to be proficient in mediating site-specific delivery of quercetin via CD44 receptor in glioblastoma cells. This targeted therapy lead to an improved therapeutic efficacy of temozolomide by modulating the brain tumor microenvironment.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Barbarisi M,Iaffaioli RV,Armenia E,Schiavo L,De Sena G,Tafuto S,Barbarisi A,Quagliariello Vdoi
10.1002/jcp.26238subject
Has Abstractpub_date
2018-10-01 00:00:00pages
6550-6564issue
10eissn
0021-9541issn
1097-4652journal_volume
233pub_type
杂志文章abstract：:Progesterone is an endogenous immunomodulator and can suppress T-cell activation during pregnancy. We have previously shown that the non-genomic effects of progesterone, especially acidification, are exerted via plasma membrane sites and suppress cellular genomic responses to mitogens. This study aimed to show that ac...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20962
更新日期：2007-05-01 00:00:00
abstract：:The actions of prostaglandins (PG) on cAMP in dispersed chief cells from guinea pig stomach were examined and compared to the actions of these agents on pepsinogen secretion. Maximal concentrations of A, B, or E prostaglandins caused a 2-5-fold increase in pepsinogen secretion and cellular cAMP. The relative order of ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041320119
更新日期：1987-07-01 00:00:00
abstract：:The effect of cell culture age and concomitant changes in cell density on the biosynthesis of sulfated-proteoglycan by rabbit articular chondrocytes in secondary monolayer culture was studied. Low density (LD, 2 d), middle density (MD, 5-7 d), and high density (HD, 12-15 d) cultures demonstrated changes in cellular mo...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041210315
更新日期：1984-12-01 00:00:00
abstract：:The endocannabinoid system (ECS) is activated at the onset of obesity and diverse metabolic diseases. Endocannabinoids mediate their physiological and behavioral effects by activating specific cannabinoid receptors, mainly cannabinoid receptor 1 (CB(1)R). Diabetic nephropathy (DN) is induced by hyperlipidemia, and ren...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22255
更新日期：2010-11-01 00:00:00
abstract：:Tyrosine hydroxylase (TH), catalyzing the conversion of tyrosine into l-DOPA, is the rate-limiting enzyme in dopamine synthesis. Defects in insulin action contribute to alterations of TH expression and/or activity in the brain and insulin increases TH levels in 1-methyl-4-phenylpyridinium (MPP+)-treated neuronal cells...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27898
更新日期：2019-07-01 00:00:00
abstract：:The retinal pigment epithelium is a monolayer of highly specialized pigmented cells located between the neural retina and the Bruch's membrane of the choroid. RPE cells play a crucial role in the maintenance and function of the underlying photoreceptors. This study introduces a spontaneously arising human retinal pigm...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25729
更新日期：2017-10-01 00:00:00
abstract：:Addition of bombesin in the presence of either forskolin or cholera toxin caused a marked (4-6 fold) enhancement of cAMP accumulation in Swiss 3T3 cells. This effect was time and concentration dependent, induced by various bombesin-like peptides and blocked by a bombesin antagonist. Enhancement of cAMP accumulation by...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041370203
更新日期：1988-11-01 00:00:00
abstract：:COVID-19, a new disease caused by the 2019-novel coronavirus (SARS-CoV-2), has swept the world and challenged its culture, economy, and health infrastructure. Forced emergence to find an effective vaccine to immunize people has led scientists to design and examine vaccine candidates all over the world. Until a vaccine...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.30041
更新日期：2020-09-09 00:00:00
abstract：:Cover: The cover image, by Michaela R. Reagan et al., is based on the Perspective A Perspective on Malignancy in the Marrow, DOI 10.1002/jcp.25860. ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25563
更新日期：2017-12-01 00:00:00
abstract：:Factors inhibiting cell growth have been isolated from different cell types. However, little information is available concerning their mode of action. A novel growth inhibitory factor of 45 kDa (IDF45) was recently purified to homogeneity from medium conditioned by 3T3 cells. This molecule was able to inhibit DNA synt...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041420219
更新日期：1990-02-01 00:00:00
abstract：:Rat thyroid follicles in both the normal and the inverted configuration have been cultured in suspension. It has been found that normal follicles (i.e., those having a structural organization similar to the follicles in vivo) express most functional properties of the thyroid gland. They trap iodide, synthesize, secret...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041260113
更新日期：1986-01-01 00:00:00
abstract：:In fertilized echinoderm eggs, the male and female pronuclei fuse to form the diploid nucleus even in the presence of aphidicolin, a specific inhibitor of eukaryotic DNA polymerase-alpha. The subsequent first cleavage is independent of chromosomes but dependent on spindle and amphiaster. The fate of DNA originally exi...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041240103
更新日期：1985-07-01 00:00:00
abstract：:Alpha-2-glycoprotein 1, zinc-binding (AZGP1), known as zinc-alpha-2-glycoprotein (ZAG), is a multifunctional secretory glycoprotein and relevant to cancer metastasis. Little is known regarding the underlying mechanisms of AZGP1 in prostate cancer (PCa). In the present study, we report that AZGP1 is an androgen-respons...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28366
更新日期：2019-08-01 00:00:00
abstract：:Interleukin (IL)-8 is released both in visceral adipose tissue and in contracting skeletal muscles. In this study, we examined cellular pathways associated with muscle hypertrophy, chosen on the basis of microRNA profiling, in differentiating rat primary skeletal muscle cells (RSkMC) treated with IL-8 (1 ng/ml) for 11...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28568
更新日期：2019-11-01 00:00:00
abstract：:We have previously reported that interferon-alpha (IFNalpha) induces apoptosis and EGF can antagonize this effect in human epidermoid cancer KB cells. Since apoptosis occurs together with cytoskeleton reorganization we have evaluated if IFNalpha and EGF could modulate cell remodeling in our experimental conditions. We...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20058
更新日期：2004-10-01 00:00:00
abstract：:Cervical cancer is a deadly gynecological malignancy in need of innovative treatment strategies. Emerging preclinical data has suggested the benefits of nanocarriers over the traditional chemotherapy for cancer treatment. In particular, gold nanoparticles are gaining popularity due to gold's inert nature, limited side...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28316
更新日期：2019-02-20 00:00:00
abstract：:GAS1 is a pleiotropic protein that has been investigated because of its ability to induce cell proliferation, cell arrest, and apoptosis, depending on the cellular or the physiological context in which it is expressed. At this point, we have information about the molecular mechanisms by which GAS1 induces proliferatio...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26226
更新日期：2018-05-01 00:00:00
abstract：:Considerable evidence has accumulated for a role of a nonsteroidal mevalonate product in the regulation of DNA replication and cell division. We report here a similar requirement for mevalonate in a nonreplicative function, that of natural killer (NK) cell cytotoxicity. Treatment of NK cells with 10 microM compactin f...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041390314
更新日期：1989-06-01 00:00:00
abstract：:Stimulation of amiloride-sensitive sodium (Na+) influx and the subsequent activation of NA+, K+-ATPase by serum or growth factors have been implicated as early events leading to initiation of cell proliferation. We recently demonstrated that amiloride inhibits thrombin-initiated DNA synthesis not by inhibiting an earl...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041200305
更新日期：1984-09-01 00:00:00
abstract：:The role of diacylglycerol (DAG) in hormonal induction of S phase was investigated in primary cultures of rat hepatocytes. In this model, several agonists that bind to G protein-coupled receptors act as comitogens when added to the cells soon after plating (i.e., in Go/early Gl phase), while the cells are most respons...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199908)180:2<203::AID-JCP8
更新日期：1999-08-01 00:00:00
abstract：:Diabetic nephropathy (DN) is a kind of microvascular complications of diabetes. Long noncoding RNAs (lnRNAs) can participate in the development of various diseases, including DN. However, the function of lncRNA NEAT1 is unclear. In our present study, we reported that NEAT1 was significantly increased in streptozotocin...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27959
更新日期：2019-08-01 00:00:00
abstract：:A mutant (A204) of Chinese hamster ovary cells (CHO-K1), which is deficient in dihydroorotate (DHO) dehydrogenase (E.C. 1.3,3.1) activity, has been isolated by a replica plating procedure. The mutant does not show a requirement for exogenously added pyrimidines. Examination of intact cells shows that the mutant accumu...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040980304
更新日期：1979-03-01 00:00:00
abstract：:Inhibition of the gap-filling, polymerizing step of excision repair by 1-beta-D-arabinofuranosylcytosine (ara-C) after irradiation with ultraviolet light in human diploid fibroblasts resulted in the formation of persistent DNA strand breaks in G1, G2, and plateau phase cells, but not in S phase cells. Addition of hydr...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041080207
更新日期：1981-08-01 00:00:00
abstract：:Parathyroid hormone-related protein (PTHrP) (107-139), in contrast to the N-terminal fragment PTHrP (1-36), has been shown to interact with the vascular endothelial growth factor (VEGF) system to modulate human osteoblast differentiation. In this study, we evaluated whether this interaction might affect human osteobla...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21547
更新日期：2008-12-01 00:00:00
abstract：:We recently derived a series of transformed cell lines by transfecting mouse bone marrow cells highly enriched for macrophage progenitors with a newly described human gene, R-myc, which has homology to the c-myc oncogene. In this report, we show that these lines share some features characteristic of cells of the monon...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041250307
更新日期：1985-12-01 00:00:00
abstract：:Development and improvement of in vitro culture system supporting self-renewal and unlimited proliferation of porcine pluripotent stem cells (pPSCs) is an indispensable process for the naïve pPSCs establishment. In this study, we modified the previous culture system and attempted to develop a novel chemically defined ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28185
更新日期：2019-01-30 00:00:00
abstract：:Osteoarthritis (OA) is a common chronic degenerative disease that affects all joints. At present, the pathological processes and mechanisms of OA are still unclear. Innate immunity, a key player in damage to the structure of the joint and the mechanism by which the host attempts to repair OA, affects all pathological ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26011
更新日期：2018-02-01 00:00:00
abstract：:The transport of L-alanine in human diploid fibroblasts was investigated. Transport measurements were performed on subcultures between the third and eighth passages with subconfluent cells growing on glass coverslips. Kinetic analysis of approximate initial rates of transport at substrate concentrations from 0.05 to 1...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041090105
更新日期：1981-10-01 00:00:00
abstract：:Polyoma transformed hamster cells (PyBHK) and SV40 transformed mouse cells (SV3T3) were transferred in culture using crystalline trypsin followed by neutralisation with soybean trypsin inhibitor. Such cells were able to proliferate freely in defined medium without any serum supplement and without any intervening perio...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040900319
更新日期：1977-03-01 00:00:00
abstract：:Histone deacetylases (HDACs) play a crucial role in the regulation of gene expression through remodeling of chromatin structures. However, the molecular mechanisms involved in this event remain unknown. In this study, we sought to examine whether HDAC inhibition-mediated protective effects involved HDAC4 sumoylation, ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24871
更新日期：2015-06-01 00:00:00
