Abstract：
:Addition of bombesin in the presence of either forskolin or cholera toxin caused a marked (4-6 fold) enhancement of cAMP accumulation in Swiss 3T3 cells. This effect was time and concentration dependent, induced by various bombesin-like peptides and blocked by a bombesin antagonist. Enhancement of cAMP accumulation by bombesin was diminished by chronic pretreatment with phorbol dibutyrate implicating the involvement of protein kinase C in the activation. Pretreatment with pertussis toxin, which uncouples protein kinase C activation from cAMP accumulation (Proc. Natl. Acad. Sci. U.S.A., 84:2282, 1987) also inhibited bombesin enhancement of cAMP. Bombesin was also shown to release E type prostaglandins into the medium, an effect which was abolished by the cyclooxygenase inhibitor indomethacin. Low concentrations (100 nM) of indomethacin partially inhibited the accumulation of cAMP by bombesin in the presence of forskolin indicating that the release of E type prostaglandins into the medium is also involved in the accumulation of cAMP by bombesin. The additive nature of PBt2-mediated down-regulation and treatment with indomethacin suggests that activation of protein kinase C and the release of E type prostaglandins provide two distinct pathways involved in the enhancement of cAMP accumulation by bombesin. Finally, bombesin in the presence of forskolin stimulated the phosphorylation of the intermediate filament component vimentin, identified in the accompanying paper as a substrate for a cAMP dependent protein kinase in intact Swiss 3T3 cells.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Millar JB,Rozengurt Edoi
10.1002/jcp.1041370203subject
Has Abstractpub_date
1988-11-01 00:00:00pages
214-22issue
2eissn
0021-9541issn
1097-4652journal_volume
137pub_type
杂志文章abstract：:The present work aimed at determining whether interleukin-6 (IL-6) produced by skeletal muscle during exercise is related, at least partly, to calcineurin activity. Rats were treated with two specific calcineurin inhibitors, cyclosporin A (CsA) and FK506, or vehicle (Vhl); they were then subjected to exhaustive treadm...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20854
更新日期：2007-03-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：2011-01-01 00:00:00
abstract：:Neuropathological disorders are increasingly associated with dysfunctions in neuronal membrane traffic and autophagy, with defects among members of the Rab family of small GTPases implicated. Mutations in the human Xq28 localized gene RAB39B have been associated with X-linked neurodevelopmental defects including macro...
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journal_title：Journal of cellular physiology
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doi：10.1002/jcp.21271
更新日期：2008-03-01 00:00:00
abstract：:The synthesis and degradation of pectoralis myosin have been investigated in chickens 15-23 days after hatching. An essentially nonreutilizable tracer amino acid was used to demonstrate differences in the rates of synthesis and degradation of myosin isolated from normal and hypertrophied, cystrophic breast tissue. The...
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abstract：:Circular RNA TCF25 (circTCF25) has been proven to be upregulated in human malignancy and correlated with tumor process. Our study aimed to unravel whether circTCF25 dictates cellular activities of osteosarcoma cells and address the possible mechanisms associated with microRNA (miR). circTCF25 and miR-206 in clinical s...
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更新日期：1993-05-01 00:00:00
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journal_title：Journal of cellular physiology
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：1999-06-01 00:00:00
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journal_title：Journal of cellular physiology
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更新日期：2020-03-01 00:00:00
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更新日期：2017-05-01 00:00:00
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journal_title：Journal of cellular physiology
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doi：10.1002/jcp.21354
更新日期：2008-06-01 00:00:00
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journal_title：Journal of cellular physiology
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更新日期：2016-04-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041250213
更新日期：1985-11-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041270219
更新日期：1986-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1002/jcp.1041530306
更新日期：1992-12-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2012-06-01 00:00:00
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更新日期：1995-10-01 00:00:00
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journal_title：Journal of cellular physiology
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更新日期：1983-08-01 00:00:00
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journal_title：Journal of cellular physiology
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更新日期：1988-04-01 00:00:00
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journal_title：Journal of cellular physiology
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更新日期：1980-09-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：1988-05-01 00:00:00
abstract：:The molecular mechanism by which thyroid hormones exert their effects on cell growth is still unknown. In this study, we used chick embryo hepatocytes at different stages of development as a model to investigate the effect of the two thyroid hormones, T3 and T4, and of their metabolite T2, on the control of cell proli...
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更新日期：2004-11-01 00:00:00
