Abstract:
:Cervical cancer is a deadly gynecological malignancy in need of innovative treatment strategies. Emerging preclinical data has suggested the benefits of nanocarriers over the traditional chemotherapy for cancer treatment. In particular, gold nanoparticles are gaining popularity due to gold's inert nature, limited side effects, good cytocompatibility, and flexibility in preparation/modification. We conjugated polyethylene glycol (PEG) with hollow gold nanospheres (HGNs) and loaded the pegylated HGNs with an anticancer drug, cisplatin to target cervical cancer. HGNs were irradiated with noninfrared laser to increase the penetration of drug into tumor tissue and improve the delivery of cisplatin. We investigated the comparative characterization studies of prepared cisplatin loaded pegylated HGNs (cis PEG-HGNs), free cisplatin, cisplatin loaded HGNs (cis-HGNs), cis PEG-HGNs without laser, and cis PEG-HGNs with laser and its effects over cervical cancer cells. Transmission electron microscopy photomicrographs confirmed the integrity of prepared HGNs. While no significant difference was observed between encapsulation efficiency and drug loading of cis-HGNs (84.6%) and cis PEG-HGNs (86.7%), the encapsulation efficiency increased almost twice in HGNs, compared with control gold nanoparticles (GNs) because of the hollow cavity in HGNs. In-vitro cytotoxicity was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay using HeLa cells. With irradiation, HGNs induced much elevated cytotoxicity. Not only HGNs were internalized by HeLa cells, they were retained in the cellular compartment. We also tested formulations in vivo and observed that the irradiated cis-HGNs and cis PEG-HGNs were most effective in regressing tumors in mice.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Guan YH,Tian M,Liu XY,Wang YNdoi
10.1002/jcp.28316subject
Has Abstractpub_date
2019-02-20 00:00:00eissn
0021-9541issn
1097-4652pub_type
杂志文章abstract::The p53 protein directly regulates the expression of the WAF1 (wild-type p53-activated fragment 1) protein which is a cyclin-dependent kinase inhibitor (CDK1). DNA damaging agents such as ionizing or UV radiation, and some chemical agents induce WAF1 in wild-type p53 containing cells, thereby halting cell cycle progre...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199603)166:3<568::AID-JCP1
更新日期:1996-03-01 00:00:00
abstract::Colon cancer remains one of the lethal malignancies in the world. Aberrant activation of canonical Wnt/β-catenin signaling pathway has been observed in colon cancer. In contrast, the non-canonical Wnt signaling functions remain obscure. Wnt5a is a representative non-canonical Wnt ligand which has gained extensive atte...
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journal_title:Journal of cellular physiology
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abstract::Sustained production of plasma proteins, notably albumin, is a reliable indicator of the differentiated state of hepatocytes. In this work, we have developed a fetal hepatocyte culture system where studying the regulation of albumin expression in proliferating liver cells. Our results show that under proliferative con...
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
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更新日期:1993-07-01 00:00:00
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更新日期:2006-03-01 00:00:00
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journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/jcp.28366
更新日期:2019-08-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041090109
更新日期:1981-10-01 00:00:00
abstract::Epidermal growth factor-like domain-containing protein 7 (EGFL7), a member of the epidermal growth factor (EGF)-like protein family, is a potent angiogenic factor expressed in many different cell types. EGFL7 plays a vital role in controlling vascular angiogenesis during embryogenesis, organogenesis, and maintaining s...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.26792
更新日期:2018-11-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199601)166:1<12::AID-JCP2>
更新日期:1996-01-01 00:00:00
abstract::Ion channels are pivotal to many aspects of sperm physiology and function. We have used the patch clamp technique to investigate the distribution of ion channels in the plasma membrane of the head of human spermatozoa. We report that three types of activity are common in the equatorial and acrosomal regions of the spe...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21153
更新日期:2007-12-01 00:00:00
abstract::Somatic cell hybrids between either normal human fibroblasts, phenotypically normal mouse fibroblasts or mouse peritoneal macrophages and HT1080 human diploid fibrosarcoma cells were studied for their ability to form tumors in nude mice. The results of this study indicate that tumorigenic behavior is expressed as a do...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040990302
更新日期:1979-06-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041530103
更新日期:1992-10-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
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更新日期:2020-03-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27158
更新日期:2019-04-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26891
更新日期:2018-01-01 00:00:00
abstract::Biosynthesis of selenium-containing proteins requires insertion of the unusual amino acid selenocysteine by alternative translation of a UGA codon, which ordinarily serves as a stop codon. In eukaryotes, selenoprotein translation depends upon one or more selenocysteine insertion sequence (SECIS) elements located in th...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20619
更新日期:2006-06-01 00:00:00
abstract::Cdk9, a member of the cyclin-dependent kinase family, is the catalytic subunit of P-TEFb, a protein kinase complex that stimulates transcriptional elongation. Cdk9, complexed with its regulatory partner cyclin T1, serves as the cellular mediator of the transactivation function of the HIV Tat protein. There are two kno...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20224
更新日期:2005-04-01 00:00:00
abstract::Proliferation of human skin fibroblasts was stimulated significantly by the presence of L-ascorbic acid 2-phosphate (Asc 2-P). The presence of Asc 2-P (0.1-1.0 mM) in the culture medium for 3 weeks enhanced the relative rate of collagen synthesis to total protein synthesis 2-fold as well as cell growth 4-fold. Coexist...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041380103
更新日期:1989-01-01 00:00:00
abstract::Pancreatic ductal adenocarcinoma (PDAC) is one of the most frequently diagnosed cancers and the fourth leading cause of cancer-related death in the United States, suggesting that there is an urgent need to design novel strategies for achieving better treatment outcome of patients diagnosed with PDAC. Our previous stud...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,收录出版
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更新日期:2013-03-01 00:00:00
abstract::The present study was to investigate the effects and action mechanisms of digoxin and ouabain on steroidogenesis in human adrenocortical NCI-H295 cells. Administration of digoxin or ouabain for 24 h decreased the basal and angiotensin II (Ang II)-stimulated release of aldosterone by NCI-H295 cells. The conversions of ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20415
更新日期:2005-12-01 00:00:00
abstract::Past work has shown that delta-opioid receptor (DOR) activation by [D-Ala(2),D-Leu(5)]-enkephalin (DADLE) attenuated the disruption of K(+) homeostasis induced by hypoxia or oxygen-glucose deprivation (OGD) in the cortex, while naltrindole, a DOR antagonist blocked this effect, suggesting that DOR activity stabilizes ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
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更新日期:2007-07-01 00:00:00
abstract::Acute lung injury, sepsis, lung inflammation, and ventilator-induced lung injury are life-threatening conditions associated with lung vascular barrier dysfunction, which may lead to pulmonary edema. Increased levels of atrial natriuretic peptide (ANP) in lung circulation reported in these pathologies suggest its poten...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21354
更新日期:2008-06-01 00:00:00
abstract::The interaction of basic FGF (bFGF) with heparin, heparan sulfate and related sugars can potentiate or antagonize bFGF activity, depending on the size of the saccharide used. Oligosaccharides based on heparin structures, as small as six sugar residues, have been demonstrated to bind to bFGF and block its activity, whi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199609)168:3<657::AID-JCP1
更新日期:1996-09-01 00:00:00