Abstract:
:Butyrate and acetate are bacterial metabolites present in the large intestine lumen. Although butyrate is well known to inhibit the in vitro proliferation of human colon carcinoma cells in a process involving the hyperacetylation of specific nuclear histones, little is known about the possible link between butyrate metabolism and its growth-inhibitory effect. In a previous study (Leschelle et al., 2000, Eur J Biochem 267: 6435-6442), we showed that butyrate accumulates and is metabolized in HT-29 Glc(-/+) cells without increasing oxygen consumption. In the present study, using the same cell line incubated with (14)C-labeled butyrate, we determined that a minor part of (14)C from butyrate was recovered in nuclear histones. Unlike butyrate, acetate exerted no effect on cell growth but was a precursor for overall net histone acetylation. Although butyrate was able to increase the cellular AMP/ADP ratio, it did not affect the ATP cell content or the adenylate charge or the oxidation of endogenous L-glutamine. Butyrate oxidation was found to be markedly sensitive to the presence of other substrates with D-glucose decreasing this oxidation and L-malate stimulating it. Furthermore, in the presence of L-malate, the growth-inhibitory effect of butyrate was significantly weaker than in its absence. From these data, we conclude that the metabolism of butyrate downstream acetyl-CoA synthesis is not involved in the butyrate antiproliferative effect. The suggestion that butyrate metabolism in mitochondria is not used in these cells as a fuel but acts as a regulator of butyrate free concentrations (thus limiting its action upon cellular targets), is discussed.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Andriamihaja M,Chaumontet C,Tome D,Blachier Fdoi
10.1002/jcp.21556subject
Has Abstractpub_date
2009-01-01 00:00:00pages
58-65issue
1eissn
0021-9541issn
1097-4652journal_volume
218pub_type
杂志文章abstract::Selenium has been associated with cancer prevention. Despite vast knowledge of selenium effect on various health conditions, functional characterization of selenium metabolic enzymes on cellular physiology has been limited. Therefore, to gain insight into the mechanisms underlying cancer prevention by selenium, we inv...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20714
更新日期:2006-10-01 00:00:00
abstract::Neuroblastoma (NBL) exists in a complex tumor-immune microenvironment. Immune cell infiltration and tumor-immune molecules play a critical role in tumor development and significantly impact the prognosis of patients. However, the molecular characteristics describing the NBL-immune interaction and their prognostic pote...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29842
更新日期:2021-01-01 00:00:00
abstract::The Belgrade rat has a hypochromic, microcytic anemia inherited as an autosomal recessive mutation. Although transferrin binds normally to reticulocytes and internalizes normally, iron accumulation into cells and heme is much slower than normal. We have investigated the role of the transferrin cycle in this mutant by ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041460317
更新日期:1991-03-01 00:00:00
abstract::We report here that heptanol (3.5 mM) induces in vitro a rapid formation of smooth endoplasmic reticulum aggregates (SERA) within isolated islets of Langerhans. SERA appeared after only 15 min of exposure to the alkanol and increased in number during the first 30 min of incubation. At that time, SERA represented 2% an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041330114
更新日期:1987-10-01 00:00:00
abstract::The basal lamina protein, laminin, has been shown to promote migration and proliferation of cultured skeletal myoblasts, resulting in increased myotube formation. However, skeletal myotubes adhere poorly to a laminin substrate, and long-term cultures of skeletal myotubes on laminin have not been achieved. We have foun...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041500205
更新日期:1992-02-01 00:00:00
abstract::Among 14 peripheral blood samples obtained from patients affected by B chronic lymphocytic leukemia (B-CLL) at initial stages (Rai 0-1) of the disease, 6 showed intermediate/high levels of Zap-70 while 8 displayed low/absent levels of Zap-70. Although Zap-70(high) and Zap-70(low) B-CLL samples displayed similar levels...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21116
更新日期:2007-10-01 00:00:00
abstract::The synthesis and degradation of pectoralis myosin have been investigated in chickens 15-23 days after hatching. An essentially nonreutilizable tracer amino acid was used to demonstrate differences in the rates of synthesis and degradation of myosin isolated from normal and hypertrophied, cystrophic breast tissue. The...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040860406
更新日期:1975-10-01 00:00:00
abstract::Although the role of several protooncogenes, including sis, myc, and myb in the regulation of growth and differentiation of vascular cells has been examined in some detail, limited information is available on the contribution of ras genes to these processes. In the present studies the influence of oncogenic ras transf...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041610312
更新日期:1994-12-01 00:00:00
abstract::Bone morphogenetic proteins (BMPs) play pivotal roles in bone and cartilage growth and repair. Through phenotypes of short-ear (se) mice, which have BMP-5 mutations, a role for BMP-5 in some specific aspects of skeletogenesis and cartilage growth is known. This report examines BMP-5 expression in the growth plate and ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21164
更新日期:2008-01-01 00:00:00
abstract::In spite of the apparent depletion of mast cells in tissues of mutant mice of W/Wv genotype, cells with many features of mast cells do develop when bone marrow cells of W/Wv mice are cultured in the presence of pokeweed mitogen-stimulated spleen cell-conditioned medium (PWM-SCM). In order to resolve this discrepancy a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041340109
更新日期:1988-01-01 00:00:00
abstract::We have previously demonstrated that insulin-like growth factor binding protein-5 (IGFBP-5) is upregulated following treatment of the mouse mammary epithelial cell line HC11 with lactogenic hormones (dexamethasone, insulin, and prolactin-DIP). In addition, we have also shown that IGFBP-5 is upregulated in mammary epit...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20587
更新日期:2006-05-01 00:00:00
abstract::The presence of inflammatory cells and their products in the tumor microenvironment plays a crucial role in the pathogenesis of a tumor. Releasing the cytokines from a host in response to infection and inflammation can inhibit tumor growth and progression. However, tumor cells can also respond to the host cytokines wi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28252
更新日期:2019-02-18 00:00:00
abstract::MicroRNAs (miRNAs) are noncoding RNA molecules that regulate gene expression at the post-transcriptional level to cause translational repression or degradation of targets. The profiles of miRNAs across stages of lactation in small ruminant species such as dairy goats is unknown. A small RNA library was constructed usi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25499
更新日期:2017-05-01 00:00:00
abstract::Cancer is a multifaceted disease that involves acquisition of genetic mutations, deletions, and amplifications as well as deregulation of epigenetic mechanisms that fine-tune gene regulation. Key epigenetic mechanisms that include histone modifications, DNA methylation, and non-coding RNA-mediated gene silencing are o...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.24387
更新日期:2013-11-01 00:00:00
abstract::Focal adhesion kinase (FAK), MAP kinases and the nuclear transcription factor Elk-1 have been reported to be implicated in the same cellular processes, however, their direct or indirect interaction and potential function(s) has not been documented. Here, we explored the association of FAK with Elk-1, the implication o...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21390
更新日期:2008-07-01 00:00:00
abstract::Islet cell transplantation, as a treatment of type 1 diabetes, has a lot of complexity such as allograft rejections and an insufficient number of donors. The liver can be used as a replacement for endogenous insulin production. Hepatocytes can inherently respond to glucose levels and secrete proteins. Utilization of m...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28352
更新日期:2019-08-01 00:00:00
abstract::Four temperature-sensitive (ts) mutants of rat 3Y1 fibroblasts, representing independent complementation groups, cease to proliferate predominantly with a 2n DNA content, at the restrictive temperature (39.8 degrees C) (temperature arrest) or at the permissive temperature (33.8 degrees C) at a confluent cell density (...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041230310
更新日期:1985-06-01 00:00:00
abstract::We investigated the actions of human placental lactogen (HPL) and human growth hormone (HGH) on [3H]thymidine incorporation and the release of immunoassayable somatomedin-C (SM-C) by isolated myoblasts, dermal fibroblasts, and costal cartilage explants taken from human fetuses at 11-21 weeks of gestation. The incorpor...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041250224
更新日期:1985-11-01 00:00:00
abstract::MicroRNA 520c and 373 (miR-520c and miR-373) have been characterized as oncogenes and play critical roles in cancer cell metastasis. However, the relationship between these two microRNAs and matrix metalloproteinases (MMPs), which are important in cancer cell metastasis, remains unknown. Here, we report new evidence i...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22993
更新日期:2012-02-01 00:00:00
abstract::EL 4-6.1 cells, variants of the murine EL4 thymoma cell line, can be activated by interleukin 1 (IL-1) or phorbol 12-myristate-13-acetate (PMA), or PMA+IL-1 to secrete interleukin 2 (IL-2) and interleukin 4 (IL-4) and to express the IL-2 receptor (IL-2R). To compare the different activation pathways, we examined the e...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041510112
更新日期:1992-04-01 00:00:00
abstract::The role of hsp27 as an inhibitor of actin polymerization was considered in the context of the actin cytoskeleton and its relationship with focal adhesion formation. The aim of this study was to evaluate the potential effects of hsp27 on focal adhesion formation as a relevant biological consequence of actin stress fib...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199812)177:4<575::AID-JCP8
更新日期:1998-12-01 00:00:00
abstract::The metabolome is a data-rich source of information concerning all the low-molecular-weight metabolites in a biofluid, which can indicate early biological changes to the host due to perturbations in metabolic pathways. Major changes can be seen after minor stimuli, which make it a valuable target for analysis. Due to ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.24002
更新日期:2012-08-01 00:00:00
abstract::The single-copy gene of human basic fibroblast growth factor (bFGF) encodes four co-expressed isoforms, with an apparent molecular weight (M(r)) of 24 kD, 22.5 kD, 22 kD, and 18 kD, co-translated from a single mRNA. As a tool for the study of the role exerted by the different bFGF isoforms in the biology of endothelia...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041610118
更新日期:1994-10-01 00:00:00
abstract::Several organic cations are actively transported by proximal renal tubules by mediated processes across both the apical and basolateral cell membranes. In order to evaluate this transport system in a cultured renal epithelium, uptake of 3H-tetraethylammonium (TEA) across the apical membrane was measured in LLCPK1 cell...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041370317
更新日期:1988-12-01 00:00:00
abstract::The next-generation sequencing (NGS) based RNA sequencing (RNA-Seq) and transcriptome profiling offers an opportunity to unveil complex biological processes. Successful RNA-Seq and transcriptome profiling requires a large amount of high-quality RNA. However, NGS-quality RNA isolation is extremely difficult from recalc...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25951
更新日期:2018-03-01 00:00:00
abstract::Ornithine decarboxylase (ODC) activity usually rises to a peak a few hours after a trophic stimulus. The stimulation of ODC has been shown to depend on extracellular calcium in several in vitro eukaryotic systems. We have investigated the effect of calcium concentration on ODC activity and have found that ODC is stimu...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041180109
更新日期:1984-01-01 00:00:00
abstract::Both mammary gland development and mammary carcinogenesis involve extensive remodeling of the mammary gland extracellular matrix. The expression of four membrane-type matrix metalloproteinases (MT-MMPs) with matrix remodeling potential in development and tumorigenesis was evaluated by in-situ hybridization on mouse ma...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20385
更新日期:2005-10-01 00:00:00
abstract::To study the role of noncollagenous proteins in bone formation, the synthesis and tissue distribution of BSP (bone sialoprotein), OPN (osteopontin) and SPARC (secreted protein acidic and rich in cysteine) were analyzed using pulse-chase and continuous labeling protocols during bone formation by cultures of rat calvari...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041520305
更新日期:1992-09-01 00:00:00
abstract::Lipocalin-2 (Lcn2; also termed neutrophil gelatinase-associated lipocalin (NGAL)) levels correlate positively with heart failure (HF) yet mechanisms via which Lcn2 contributes to the pathogenesis of HF remain unclear. In this study, we used coronary artery ligation surgery to induce ischemia in wild-type (wt) mice and...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25672
更新日期:2017-08-01 00:00:00
abstract::P2Y(2) receptor expression is increased in intestinal epithelial cells (IECs) during inflammatory bowel diseases (IBDs). In this context, P2Y(2) stimulates PGE(2) release by IECs, suggesting a role in wound healing. For this study, we have used the non-cancerous IEC-6 cell line. IEC-6 cell migration was determined usi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24109
更新日期:2013-01-01 00:00:00