Abstract：
:The basal lamina protein, laminin, has been shown to promote migration and proliferation of cultured skeletal myoblasts, resulting in increased myotube formation. However, skeletal myotubes adhere poorly to a laminin substrate, and long-term cultures of skeletal myotubes on laminin have not been achieved. We have found that cultured satellite cells from bupivacaine-damaged rat skeletal muscle actively proliferate and differentiate on a diluted Matrigel substrate composed of laminin, type IV collagen, heparan sulfate proteoglycan, and entactin. Myotubes cultured on diluted Matrigel are contractile and have never been observed to detach from the culture dish; rather, myotubes generally atrophy after 2-3 weeks in culture. Antibodies directed against the various protein components of Matrigel were used to determine the role of each component in enhancing muscle differentiation. Anti-laminin impaired satellite cell adhesion, whereas antibodies against either type IV collagen or heparan sulfate proteoglycan had no effect. Anti-entactin did not inhibit attachment, proliferation, or fusion of cultured satellite cells; however, myotubes exposed to anti-entactin failed to adhere to the culture dish after spontaneous myotube contractions began. We conclude that entactin is responsible for long-term maintenance and maturation of contractile skeletal myotubes on a diluted Matrigel substrate. This is the first study to assign a biological function for entactin in myogenesis.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Funanage VL,Smith SM,Minnich MAdoi
10.1002/jcp.1041500205subject
Has Abstractpub_date
1992-02-01 00:00:00pages
251-7issue
2eissn
0021-9541issn
1097-4652journal_volume
150pub_type
杂志文章abstract：:We report here that the androgen receptor (AR) and ABCB1 are upregulated in a model of acquired taxol resistance (txr) in ovarian carcinoma cells. AR silencing sensitizes txr cells to taxol threefold, whereas ectopic AR expression in AR-null HEK293 cells induces resistance to taxol by 1.7-fold. AR activation using the...
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abstract：:The extent to which autolytic microbial enzymes are involved in the fate of microorganisms ingested by phagocytes has not been determined. It is known, however, that activation of degradative enzymes occurs during certain microbicidal events. We examined the possible role of the pneumococcal autolytic enzyme (an N-ace...
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abstract：:Tumor cells secrete extracellular vesicles (EVs) for intercellular communication. EVs by transporting different proteins, nucleic acids, and lipids contribute to affect target cell function and fate. EVs which originate directly from multivesicular bodies so-called exosomes have dramatically fascinated the attention ...
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更新日期：1987-12-01 00:00:00
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更新日期：1978-12-01 00:00:00
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更新日期：2019-08-01 00:00:00
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更新日期：1984-04-01 00:00:00
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journal_title：Journal of cellular physiology
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更新日期：2006-03-01 00:00:00
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journal_title：Journal of cellular physiology
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更新日期：2020-12-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199702)170:2<209::AID-JCP1
更新日期：1997-02-01 00:00:00
abstract：:Despite the advantages of transplantation of umbilical cord blood's (UCB's) hematopoietic stem cells (uHSCs) for hematologic malignancy treatment, there are two major challenges in using them: (a) Insufficient amount of uHSCs in a UCB unit; (b) a defect in uHSCs homing to bone marrow (BM) due to loose binding of their...
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pub_type： 杂志文章
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更新日期：2019-12-01 00:00:00
abstract：:The single-stranded DNA and RNA binding protein, Puralpha, has recently received special attention as this protein, by associating with the specific nucleotide sequence (GGN repeats) and/or several important cellular and viral proteins regulates crucial biological events such as transcription, replication, and cell pr...
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更新日期：2001-03-01 00:00:00
abstract：:Grb10 is a member of a superfamily of adapter proteins that includes Grb10, 7, 14, and a protein of Caenorhabditis elegans called Mig10. Grb10 proteins are binding partners for several trans-membrane tyrosine-kinase receptors, including the insulin-like growth factor receptor (IGF-IR) and the insulin receptor (IR). Ma...
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更新日期：2003-12-01 00:00:00
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更新日期：2015-12-01 00:00:00
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更新日期：2018-11-01 00:00:00
abstract：:Established RAW264.7 cell lines for osteoclastic differentiation has been widely engaged in bone homeostasis research, however, the efficacy of RANKL independently stimulating has rarely been defined, because protocols were usually developed and modified by various laboratories. Otherwise, problematic issues are also ...
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更新日期：2019-02-01 00:00:00
abstract：:The antibody-secreting murine hybridoma, CC9C10, was grown in batch culture in a medium containing 20 mM glucose and 2 mM glutamine. After 2 days of exponential growth, the glutamine content of the medium was completely depleted, whereas the glucose content was reduced to 60% of the original concentration. The glucose...
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更新日期：1994-10-01 00:00:00
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更新日期：1991-10-01 00:00:00
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更新日期：2002-06-01 00:00:00
abstract：:Alkaptonuria (AKU) is a rare disease correlated with deficiency of the enzyme homogentisate 1,2 dioxygenase, which causes homogentisic acid (HGA) accumulation. HGA is subjected to oxidation/polymerization reactions, leading to the production of a peculiar melanin-like pigmentation (ochronosis) after chronic inflammati...
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