Involvement of protein phosphatase 2A in the maintenance of E-cadherin-mediated cell-cell adhesion through recruitment of IQGAP1.


:Serine/threonine protein phosphatase (PP) 2A regulates many biological processes, however it remains unclear whether PP2A participates in cadherin-mediated cell-cell adhesion. We show here that the core enzyme of PP2A (PP2A-AC) is localized in the cell-cell adhesion sites between adjacent cells and associated with the E-cadherin-catenins complex in non-malignant human mammary epithelial (HME) cells at confluence. Treatment of the cells with either okadaic acid (OA), an inhibitor of PP2A, or siRNA for the regulatory subunit A of PP2A (PP2A-A) caused disruption of cell-cell adhesion and F-actin assembly, without affecting the complex formation of E-cadherin with beta- and alpha-catenins. While a small GTPase Rac and its effector IQGAP1 were associated with the E-cadherin-catenins complex, either OA or PP2A-A siRNA concomitantly induced the dissociation of IQGAP1, but not Rac, from the complex and the internalization of E-cadherin from the cell surface. We therefore propose that PP2A plays a crucial role in the maintenance of cell-cell adhesion through recruitment of IQGAP1 to the Rac-bound E-cadherin-catenins complex.


J Cell Physiol


Takahashi K,Nakajima E,Suzuki K





Has Abstract


2006-03-01 00:00:00












  • γ-d-Glutamyl-meso-diaminopimelic acid induces autophagy in bovine hepatocytes during nucleotide-binding oligomerization domain 1-mediated inflammation.

    abstract::Autophagy is a crucial cellular homeostatic process and an important part of the host defense system. Dysfunction in autophagy enhances tissue susceptibility to infection and multiple diseases. However, the role of nucleotide oligomerization domain 1 (NOD1) in autophagy in bovine hepatocytes is not well known. Therefo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Roy AC,Chang G,Roy S,Ma N,Gao Q,Shen X

    更新日期:2020-12-24 00:00:00

  • Purification and characterization of a motility initiating protein from caprine epididymal plasma.

    abstract::Numerous reports have appeared on the occurrence of undefined protein factors in male reproductive fluids that promote motility of mature sperm and initiate forward motility in the immature (immotile) caput-epididymal sperm. This study reports for the first time purification to apparent homogeneity of a motility initi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Jaiswal BS,Das K,Saha S,Dungdung SR,Majumder GC

    更新日期:2010-01-01 00:00:00

  • Growth kinetics as a function of ploidy in diploid, tetraploid, and octaploid smooth muscle cells derived from the normal rat aorta.

    abstract::The smooth muscle cell population in major arteries of humans and experimental animals is heterogeneous with regard to cellular DNA content. A proportion of cells has polyploid DNA content and this proportion increases with normal aging and with hypertension. We have isolated pure populations of rat aortic smooth musc...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Rosen EM,Goldberg ID,Shapiro HM,Zoller LC,Myrick KV,Levenson SE,Halpin PA

    更新日期:1985-12-01 00:00:00

  • MicroRNA implications in the etiopathogenesis of ankylosing spondylitis.

    abstract::Ankylosing spondylitis (AS) is a chronic immune-mediated inflammatory disease that affects both axial and peripheral skeletons as well as soft tissues. Recent investigations offer that disease pathogenesis is ascribed to a complex interplay of genetic, environmental, and immunological factors. Until now, there is no a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Mohammadi H,Hemmatzadeh M,Babaie F,Gowhari Shabgah A,Azizi G,Hosseini F,Majidi J,Baradaran B

    更新日期:2018-08-01 00:00:00

  • Epidermal growth factor stimulation of prostacyclin production by cultured aortic smooth muscle cells: requirement for increased cellular calcium levels.

    abstract::We have examined the ability of epidermal growth factor (EGF) to regulate prostacyclin production by cultured A10 smooth muscle cells. EGF by itself had no effect on prostacyclin production, but it augmented the response to arg8-vasopressin. An AGF stimulation of prostacyclin production was also observed in the presen...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Blay J,Hollenberg MD

    更新日期:1989-06-01 00:00:00

  • Potassium transport in normal and transformed mouse 3T3 cells.

    abstract::The components of unidirectional K influx and efflux have been investigated in the 3T3 cell and the SV40 transformed 3T3 cell in expontntial and stationary growth phase. Over the cell densities used for transport experiments the 3T3 cell goes from exponential growth to density dependent inhibition of growth (4 X 10(4)...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Spaggiare S,Wallach MJ,Tupper JT

    更新日期:1976-11-01 00:00:00

  • Epithelial cell adhesion molecule fragments and signaling in primary human liver cells.

    abstract::Epithelial Cell Adhesion Molecule (EpCAM), or CD326, is a trans-membrane glycoprotein expressed by multiple normal epithelia as well as carcinoma. Human hepatic stem cells and bile duct epithelium of the liver are EpCAM positive. In tumor cell lines, its intracellular domain can be released after cleavage of the extra...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Gerlach JC,Foka HG,Thompson RL,Gridelli B,Schmelzer E

    更新日期:2018-06-01 00:00:00

  • Synergism between gangliosides and basic fibroblastic growth factor in favouring survival, growth, and motility of capillary endothelium.

    abstract::The experiments reported were motivated by the observation that in vivo gangliosides promoted angiogenesis when the dose of the angiogenic factor was too low to be effective (Ziche et al.: Laboratory Investigation 61:629-634, 1989). As an approach to understanding the mechanism of this modulatory effect, we analysed t...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: De Cristan G,Morbidelli L,Alessandri G,Ziche M,Cappa AP,Gullino PM

    更新日期:1990-09-01 00:00:00

  • Endogenous thiol levels in heterogeneous murine tumor cells as a function of the physiological state and the response to X-irradiation.

    abstract::The endogenous thiols (PSH, protein sulfhydryls; NPSH, nonprotein sulfhydryls; and GSH, glutathione) were measured in the 66 and 67 murine carcinoma cells growing under different physiological conditions in vitro (e.g., proliferation, P; nutrient-deprived quiescence QI; and QI cells stimulated by refeeding the monolay...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dethlefsen LA,Biaglow JE,Peck VM,Ridinger DN

    更新日期:1987-07-01 00:00:00

  • Protein kinases and adherens junction dynamics in the seminiferous epithelium of the rat testis.

    abstract::Earlier studies in multiple epithelia have shown that cell-cell actin-based adherens junction (AJ) dynamics are regulated, at least in part, by the interplay of kinases and phosphatases that determines the intracellular phosphoprotein content. Yet it is virtually unknown regarding the role of protein kinases in Sertol...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lee NP,Cheng CY

    更新日期:2005-02-01 00:00:00

  • Activation of β-catenin in Col2-expressing chondrocytes leads to osteoarthritis-like defects in hip joint.

    abstract::Although osteoarthritis (OA) in the hip joint is a common and debilitating degenerative disease, the precise molecular mechanisms underlying its pathological process remains unclear. This study sets out to investigate whether β-catenin plays a critical role in hip OA pathogenesis. Here, we showed overexpressed β-caten...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Xia C,Wang P,Fang L,Ge Q,Zou Z,Dong R,Zhang P,Shi Z,Xu R,Zhang L,Luo C,Ying J,Xiao L,Shen J,Chen D,Tong P,Jin H

    更新日期:2019-08-01 00:00:00

  • Circulating and tissue resident endothelial progenitor cells.

    abstract::Progenitor cells for the endothelial lineage have been widely investigated for more than a decade, but continue to be controversial since no unique identifying marker has yet been identified. This review will begin with a discussion of the basic tenets originally proposed for proof that a cell displays properties of a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Basile DP,Yoder MC

    更新日期:2014-01-01 00:00:00

  • Stem cell factor can overcome inhibition of highly purified human burst-forming units-erythroid by interferon gamma.

    abstract::Highly purified human blood burst-forming units-erythroid (BFU-E) were used to study the effects of interferon gamma (IFN gamma). IFN gamma inhibited erythroid colony formation, cell proliferation, and differentiation of day 3 to day 6 mature BFU-E in a dose-dependent manner. The primitive BFU-E (day 1 and day 2 cells...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dai CH,Krantz SB,Kollar K,Price JO

    更新日期:1995-11-01 00:00:00

  • Isoprenoids and astroglial cell cycling: diminished mevalonate availability and inhibition of dolichol-linked glycoprotein synthesis arrest cycling through distinct mechanisms.

    abstract::Primary astroglial cultures were used to compare the relationships to cell cycling of dolichol-linked glycoprotein synthesis, and of availability of mevalonate, the precursor of dolichol and other isoprenoid lipids. With shift-up to 10% serum (time 0) after 48 h of serum depletion, the proportion of cells in S phase (...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Langan TJ,Slater MC

    更新日期:1991-11-01 00:00:00

  • Critical role for intracellular calcium in tight junction biogenesis.

    abstract::Using the Madin Darby canine kidney (MDCK) cell "calcium switch," we have previously demonstrated that, as MDCK cells establish contact and ultimately form tight junctions, there are marked global and localized changes in intracellular calcium at the sites of cell-cell contact (Nigam et al., 1992, Proc. Natl. Acad. Sc...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Stuart RO,Sun A,Panichas M,Hebert SC,Brenner BM,Nigam SK

    更新日期:1994-06-01 00:00:00

  • Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPase(old-35)).

    abstract::As a strategy to identify gene expression changes affected by human polynucleotide phosphorylase (hPNPase(old-35)), we performed gene expression analysis of HeLa cells in which hPNPase(old-35) was overexpressed. The observed changes were then compared to those of HO-1 melanoma cells in which hPNPase(old-35) was stably...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sokhi UK,Bacolod MD,Emdad L,Das SK,Dumur CI,Miles MF,Sarkar D,Fisher PB

    更新日期:2014-12-01 00:00:00

  • Selenium deficiency in cultured adrenocortical cells: restoration of glutathione peroxidase and resistance to hydroperoxides on addition of selenium.

    abstract::Cultured bovine adrenocortical cells were previously shown to be functionally deficient in selenium and vitamin E when grown in medium supplemented with fetal bovine serum. In the present experiments, the lack of significant bioavailable amounts of selenium in the medium was demonstrated by the finding of only low lev...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hornsby PJ,Pearson DW,Autor AP,Aldern KA,Harris SE

    更新日期:1985-04-01 00:00:00

  • Platelet-derived growth factor-induced disruption of gap junctional communication and phosphorylation of connexin43 involves protein kinase C and mitogen-activated protein kinase.

    abstract::Previously we showed a rapid and transient inhibition of gap junctional communication (GJC) by platelet-derived growth factor (PDGF) in T51B rat liver epithelial cells expressing wild-type platelet-derived growth factor beta receptors (PDGFrbeta). This action of PDGF correlated with the hyperphosphorylation of the gap...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hossain MZ,Ao P,Boynton AL

    更新日期:1998-08-01 00:00:00

  • Identification of microRNAs controlling human ovarian cell steroidogenesis via a genome-scale screen.

    abstract::The aim of our studies was to identify miRNAs affecting the release of the major ovarian steroid hormones progestagen, androgen and estrogen by human ovarian cells. The effect of transfection of cultured primary ovarian granulosa cells with 80 different gene constructs encoding human pre-miRNAs on release of progester...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sirotkin AV,Ovcharenko D,Grossmann R,Lauková M,Mlyncek M

    更新日期:2009-05-01 00:00:00

  • Combination of low O(2) concentration and mesenchymal stromal cells during culture of cord blood CD34(+) cells improves the maintenance and proliferative capacity of hematopoietic stem cells.

    abstract::The physiological approach suggests that an environment associating the mesenchymal stromal cells (MSC) and low O(2) concentration would be most favorable for the maintenance of hematopoietic stem cells (HSCs) in course of ex vivo expansion of hematopoietic grafts. To test this hypothesis, we performed a co-culture of...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hammoud M,Vlaski M,Duchez P,Chevaleyre J,Lafarge X,Boiron JM,Praloran V,Brunet De La Grange P,Ivanovic Z

    更新日期:2012-06-01 00:00:00

  • Cell cycle reentry of mammalian fibroblasts is accompanied by the sustained activation of p44mapk and p42mapk isoforms in the G1 phase and their inactivation at the G1/S transition.

    abstract::Mitogen-activated protein (MAP) kinases are serine/threonine kinases that are rapidly activated in response to mitogenic stimuli. Here we examined the enzymatic activity and phosphorylation state of the individual p44mapk and p42mapk isoforms during early G1 and late G1 phase of the mammalian cell cycle. Release of fi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Meloche S

    更新日期:1995-06-01 00:00:00

  • Mechanisms of apoptosis modulation by curcumin: Implications for cancer therapy.

    abstract::Cancer incidences are growing and cause millions of deaths worldwide. Cancer therapy is one of the most important challenges in medicine. Improving therapeutic outcomes from cancer therapy is necessary for increasing patients' survival and quality of life. Adjuvant therapy using various types of antibodies or immunomo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Mortezaee K,Salehi E,Mirtavoos-Mahyari H,Motevaseli E,Najafi M,Farhood B,Rosengren RJ,Sahebkar A

    更新日期:2019-08-01 00:00:00

  • In vitro paracrine regulation of human keratinocyte growth by fibroblast-derived insulin-like growth factors.

    abstract::Human keratinocytes isolated from a skin biopsy and cultured in vitro on a feeder-layer of irradiated fibroblasts reconstitute a stratified squamous epithelium suitable for grafting onto patients suffering from large burn wounds. Since conditioned medium from 3T3-J2 cells can partially substitute for the intact feeder...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Barreca A,De Luca M,Del Monte P,Bondanza S,Damonte G,Cariola G,Di Marco E,Giordano G,Cancedda R,Minuto F

    更新日期:1992-05-01 00:00:00

  • Ameliorative potential of resveratrol on proinflammatory cytokines, hyperglycemia mediated oxidative stress, and pancreatic beta-cell dysfunction in streptozotocin-nicotinamide-induced diabetic rats.

    abstract::Chronic exposure of pancreatic beta-cells to supraphysiologic glucose causes adverse beta-cell dysfunction. Thus, the present study was aimed to investigate the hypothesis that oral administration of resveratrol attenuates hyperglycemia, proinflammatory cytokines and antioxidant competence and protects beta-cell ultra...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Palsamy P,Subramanian S

    更新日期:2010-08-01 00:00:00

  • Culture-induced increase in acidic and basic fibroblast growth factor activities and their association with the nuclei of vascular endothelial and smooth muscle cells.

    abstract::The activity of acidic and basic fibroblast growth factor-like mitogens (aFGF, bFGF) extracted from cultured bovine aortic endothelial (BAEC) and rat aortic smooth muscle cells (SMC) was compared with that of freshly isolated cells from the same tissues. Extracts of subendothelial extracellular matrix (ECM) and cell l...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Speir E,Sasse J,Shrivastav S,Casscells W

    更新日期:1991-05-01 00:00:00

  • Effect of antioxidants on lipopolysaccharide-stimulated induction of mangano superoxide dismutase mRNA in bovine pulmonary artery endothelial cells.

    abstract::Generation of reactive oxygen species (ROS) is a common event in the pathogenesis of acute lung injury. Endothelial cells may be both a target and a source of the ROS. Exposure of bovine pulmonary endothelial cells (BPAEC) to lipopolysaccharide (LPS) has been shown to result in intracellular generation of both ROS and...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mitchell J,Jiang H,Berry L,Meyrick B

    更新日期:1996-11-01 00:00:00

  • Curcumin ameliorates atherosclerosis through upregulation of miR-126.

    abstract::The potential usage of curcumin in diverse human diseases has been widely studied, including arteriosclerosis (AS). This study focused on investigating the relationship between curcumin and AS-associated microRNA, which may provide a better understanding of curcumin in a different mechanism. Human microvascular endoth...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Li Y,Tian L,Sun D,Yin D

    更新日期:2019-11-01 00:00:00

  • The use of 5-bromodeoxyuridine and irradiation for the estimation of the myoblast and myocyte content of primary rat heart cell cultures.

    abstract::A method for killing dividing cells (Puck and Kao, '67) was adapted for the elimination of dividing heart muscle cells (myoblasts) in cultures. We have used this method to demonstrate their presence and to estimate their number as well as the number of nondividing heart muscle cells (myocytes) in the neo-natal rat hea...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Masse MJ,Harary I

    更新日期:1980-11-01 00:00:00

  • Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation.

    abstract::Fibroblastic and epithelioid clones have been isolated from the normal rat kidney line, NRK. These clones were studied for their ability to bind epidermal growth factor (EGF), susceptibility to transformation by mouse sarcoma virus (MSV), and alteration in EGF binding upon sarcoma virus transformation. The epithelioid...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: de Larco JE,Todaro GJ

    更新日期:1978-03-01 00:00:00

  • Inhibition of late endosome-lysosome fusion: studies on the mechanism by which isotonic-K+ buffers alter intracellular ligand movement.

    abstract::Incubation of alveolar macrophages or hepatocytes in media in which Na+ is replaced by K+ ("isotonic-K buffer") inhibited the movement of internalized ligand from late endosomes to lysosomes (Ward et al.: Journal of Cell Biology 110:1013-1022, 1990). In this study we investigate the mechanism responsible for the isoto...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ward DM,Hackenyos DP,Davis-Kaplan S,Kaplan J

    更新日期:1990-12-01 00:00:00