Abstract:
:When human diploid fibroblasts IMR-90 are cultured in routinely used medium (Eagle's basal medium supplemented with 10% fetal calf serum), sulfhydryl compounds appear in the medium. The major component of these sulfhydryl compounds is cysteine, and it is shown that a part of medium cystine is converted into cysteine by the cells. It is also shown that the sulfhydryl groups of serum albumin, which are masked and barely detectable before the culture, are restored. Probably cysteine formed by the cells reacts with serum albumin to give rise to the protein sulfhydryl groups via sulfhydryl-disulfide exchange reactions. Total sulfhydryl concentrations in the medium are maintained in a considerable level throughout the culture, and a possible physiological function of these sulfhydryl groups is discussed.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Bannai S,Ishii Tdoi
10.1002/jcp.1041040211subject
Has Abstractpub_date
1980-08-01 00:00:00pages
215-23issue
2eissn
0021-9541issn
1097-4652journal_volume
104pub_type
杂志文章abstract::The amino acid pools in Chinese hamster lung V79 cells were measured as a function of time during hyperthermic exposure at 40.5 degrees and 45.0 degrees C. Sixteen of the 20 protein amino acids were present in sufficient quantity to measure accurately. The total amino acid pool and all individual amino acids, except g...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041350120
更新日期:1988-04-01 00:00:00
abstract::The methylation of DNA is an epigenetic modification that can play an important role in the control of gene expression in mammalian cells. The enzyme involved in this process is DNA methyltransferase, which catalyzes the transfer of a methyl group from S-adenosyl-methionine to cytosine residues to form 5-methylcytosin...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-4652(200005)183:2<145::AID-JCP1
更新日期:2000-05-01 00:00:00
abstract::Fibroblastic and epithelioid clones have been isolated from the normal rat kidney line, NRK. These clones were studied for their ability to bind epidermal growth factor (EGF), susceptibility to transformation by mouse sarcoma virus (MSV), and alteration in EGF binding upon sarcoma virus transformation. The epithelioid...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040940311
更新日期:1978-03-01 00:00:00
abstract::Transforming growth factor beta type 1 (TGF-beta 1) was reacted with NHS-biotin to yield a derivative of TGF-beta 1 which was biotinylated on lysine residues. The biotinylated form of TGF-beta 1 was separated from the unreacted material by reverse phase chromatography. In three separate bioassays, the derivatized pept...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041410125
更新日期:1989-10-01 00:00:00
abstract::Myocardium infarction is one of the most deathly cardiovascular diseases. It is characterized by myocardium ischemia as a result of nutrients depletion and hypoxia. The cell can respond to this injury by autophagy or apoptosis, which determines the evolution and possible recovery of the myocardium infarction. Lipids p...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25344
更新日期:2016-10-01 00:00:00
abstract::Mast cells are central regulators of allergic inflammation through production of various chemical mediators and cytokines. Bacterial infection occasionally worsens allergic inflammation. Although the exact mechanism of this phenomenon remains unclear, we have previously reported that LPS stimulates mast cells to produ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21101
更新日期:2007-10-01 00:00:00
abstract::The prevalence of chronic hyperglycemia and its complications, imposing a critical burden on the worldwide economy and the global healthcare system, is a pressing issue. Mounting evidence indicates that oxidative stress and hypoxia, two noticeable features of hyperglycemia, play a joint crucial role in mediating cellu...
journal_title:Journal of cellular physiology
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更新日期:2019-08-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199905)179:2<115::AID-JCP1
更新日期:1999-05-01 00:00:00
abstract::The effects of src oncogene expression on epidermal growth factor (EGF) receptors have been investigated in mouse 3T3 and rat-1 fibroblasts. Transformation of both cell types with src resulted in marked reductions in cellular EGF receptor levels, as assayed by either 125I-EGF binding or immunoprecipitation of receptor...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041310318
更新日期:1987-06-01 00:00:00
abstract::Abnormal glucose handling in the proximal tubule may play an important role in the development of diabetic nephropathy. Thus, the present study was designed to examine the effect of high glucose on alpha-methyl-D-glucopyranoside (alpha-MG) uptake and its signaling pathways in the primary cultured rabbit renal proximal...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(200006)183:3<355::AID-JCP8
更新日期:2000-06-01 00:00:00
abstract::Tyrosine hydroxylase (TH), catalyzing the conversion of tyrosine into l-DOPA, is the rate-limiting enzyme in dopamine synthesis. Defects in insulin action contribute to alterations of TH expression and/or activity in the brain and insulin increases TH levels in 1-methyl-4-phenylpyridinium (MPP+)-treated neuronal cells...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27898
更新日期:2019-07-01 00:00:00
abstract::Osteosarcoma (OS) is a primary highly malignant tumor of bone, affecting predominately adolescents and young adults between 10 and 20 years of age. OS is characterized by an extremely aggressive clinical course, with a rapid development of metastasis to the lung and distant bones. ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24996
更新日期:2015-11-01 00:00:00
abstract::The growth of human epithelial cells is stimulated by cholera toxin and analogues of cyclic AMP, while the growth of breast derived fibroblasts is inhibited. These compounds have little effect on DNA synthesis in the absence of other mitogens but show a synergistic effect with serum and/or EGF. The results suggest tha...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041020306
更新日期:1980-03-01 00:00:00
abstract::Embryo implantation is essential for normal pregnancy, and the process of decidualization is critical for embryo implantation. However, the mechanism of decidualization during early pregnancy is still unknown. Forkhead box O3a (FOXO3a) is the most important functional transcription factor of the forkhead box family an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27167
更新日期:2019-04-01 00:00:00
abstract::CRSBP-1 (mammalian LYVE-1) is a membrane glycoprotein highly expressed in lymphatic endothelial cells (LECs). It has multiple ligands, including hyaluronic acid (HA) and growth factors/cytokines (e.g., PDGF-BB and VEGF-A) containing CRS motifs (clusters of basic amino-acid residues). The ligand binding activities are ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26159
更新日期:2018-04-01 00:00:00
abstract::Osteoclasts are multinucleated cells formed by fusion of preosteoclasts (POCs) derived from cells of the monocyte/macrophage lineage. We have reported a culture system that supports the formation of POCs from stroma-depleted rat bone marrow cells. Global gene expression analysis of this culture system identified genes...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26147
更新日期:2018-04-01 00:00:00
abstract::There are two known phosphorylation-mediated inactivation mechanisms for TRPC3 channels. Protein kinase G (PKG) inactivates TRPC3 by direct phosphorylation on Thr-11 and Ser-263 of the TRPC3 proteins, and protein kinase C (PKC) inactivates TRPC3 by phosphorylation on Ser-712. In the present study, we explored the rela...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20567
更新日期:2006-05-01 00:00:00
abstract::To clarify the manner by which erythropoietin (EP), stem cell factor (SCF), or insulin-like growth factor I (IGF-I) regulate erythropoiesis, apoptosis of human erythroid progenitor cells was investigated. Human burst-forming units-erythroid (BFU-E) partially purified from peripheral blood were cultured for 6 days to g...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041560207
更新日期:1993-08-01 00:00:00
abstract::Numerous studies have reported that long noncoding RNA (lncRNA) dysregulation is involved in the progression of many malignant tumors, including glioma. The lncRNA ZNFX1 antisense RNA 1 (ZFAS1) plays an oncogenic role in various malignant tumors, such as gastric cancer and hepatocellular carcinoma. However, the underl...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040970302
更新日期:1978-12-01 00:00:00
abstract::C1q/tumor necrosis factor-related protein-6 (CTRP6) is a newly identified adipokine involved in diverse biological processes. However, its role in salivary glands remains unknown. Here, we demonstrated that CTRP6 was mainly distributed in the nuclei, apicolateral membranes, and cytoplasm of human submandibular glands ...
journal_title:Journal of cellular physiology
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更新日期:2021-01-05 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040860307
更新日期:1975-12-01 00:00:00
abstract::Leukotriene C4 is an arachidonic acid metabolite and an important mediator of inflammation and anaphylaxis that is known to induce production of prostacyclin in endothelial cells. The goal of this study was to examine the signal transduction mechanisms activated by leukotriene C4 stimulation. Formation of inositol pho...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199810)177:1<103::AID-JCP1
更新日期:1998-10-01 00:00:00
abstract::Nonalcoholic fatty liver disease (NAFLD) is one of the main liver diseases today, and may progress to steatohepatitis, cirrhosis, and hepatocellular carcinoma. Some studies have shown the beneficial effects of aerobic exercise on reversing NAFLD. To verify whether chronic aerobic exercise improves the insulin resistan...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25440
更新日期:2017-05-01 00:00:00
abstract::We previously demonstrated that 4-oxoretinol (4-oxo-ROL) activated retinoic acid receptors (RARs) in F9 stem cells. We showed that 4-oxo-ROL inhibited the proliferation of normal human mammary epithelial cells (HMECs). To understand the mechanisms by which 4-oxo-ROL regulates HMEC growth we examined gene expression pr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21824
更新日期:2009-09-01 00:00:00
abstract::Experiments were carried out to test the hypothesis that cell spreading and phagocytosis are similar cell responses to different-sized substrata. The following morphological and biochemical studies provided evidence for this supposition. Cells phagocytosed 1.09-micron and 5.7-micron latex beads, but were unable to com...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041190110
更新日期:1984-04-01 00:00:00
abstract::As a strategy to identify gene expression changes affected by human polynucleotide phosphorylase (hPNPase(old-35)), we performed gene expression analysis of HeLa cells in which hPNPase(old-35) was overexpressed. The observed changes were then compared to those of HO-1 melanoma cells in which hPNPase(old-35) was stably...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24645
更新日期:2014-12-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199806)175:3<348::AID-JCP1
更新日期:1998-06-01 00:00:00
abstract::COVID-19, a new disease caused by the 2019-novel coronavirus (SARS-CoV-2), has swept the world and challenged its culture, economy, and health infrastructure. Forced emergence to find an effective vaccine to immunize people has led scientists to design and examine vaccine candidates all over the world. Until a vaccine...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.30041
更新日期:2020-09-09 00:00:00
abstract::The tuftelin protein isoforms undergo post-translation modifications, and are ubiquitously expressed in various tissues in embryos, adults, and tumors. Developmental and pathological studies suggested an apparent correlation between oxygen deprivation and tuftelin expression. The aim of the study was therefore to inve...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22318
更新日期:2011-01-01 00:00:00