Generation of insulin-producing hepatocyte-like cells from human Wharton's jelly mesenchymal stem cells as an alternative source of islet cells.

Abstract:

:Islet cell transplantation, as a treatment of type 1 diabetes, has a lot of complexity such as allograft rejections and an insufficient number of donors. The liver can be used as a replacement for endogenous insulin production. Hepatocytes can inherently respond to glucose levels and secrete proteins. Utilization of mesenchymal stem cells for curing diabetes represents a major focus of recent investigations. As a new choice for transplantation, we have proposed glucose-regulated insulin-producing hepatocyte-like cells, which produce insulin dependent on glucose levels. We have transfected human Wharton's jelly mesenchymal stem cells with the special construct, which included homology arms and glucose-responsive elements upstream of the minimum liver-type pyruvate kinase promoter-directed insulin gene. Then, we have differentiated these transfected cells to hepatocyte-like cells by using serial exposure of different inducing material and exogenous growth factors. Immunofluorescence analyses have demonstrated the expression of albumin, cytokeratin-18, Hep-Par1, α-fetoprotein, and insulin. The expression of hepatocyte marker genes in the differentiated cells was confirmed by reverse-transcription polymerase chain reaction. Interestingly, flow cytometry results showed that approximately 60% of the insulin-producing hepatocyte-like cells were simultaneously cytochrome P450 3A4 (CYP3A4) and insulin positive. CYP3A4 is a significant enzyme found in mature liver tissue. This confirmed that the differentiation and the transfection procedures were done correctly. They were functionally active by releasing insulin in response to elevated glucose concentrations in vitro. These applicable cells could be used in the liver for cell therapy of diabetes.

journal_name

J Cell Physiol

authors

Beikmohammadi L,Bandehpour M,Hashemi SM,Kazemi B

doi

10.1002/jcp.28352

subject

Has Abstract

pub_date

2019-08-01 00:00:00

pages

17326-17336

issue

10

eissn

0021-9541

issn

1097-4652

journal_volume

234

pub_type

杂志文章
  • Circulating and tissue resident endothelial progenitor cells.

    abstract::Progenitor cells for the endothelial lineage have been widely investigated for more than a decade, but continue to be controversial since no unique identifying marker has yet been identified. This review will begin with a discussion of the basic tenets originally proposed for proof that a cell displays properties of a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.24423

    authors: Basile DP,Yoder MC

    更新日期:2014-01-01 00:00:00

  • Electromagnetic fields at mobile phone frequency induce apoptosis and inactivation of the multi-chaperone complex in human epidermoid cancer cells.

    abstract::The exposure to non-thermal microwave electromagnetic field (MW-EMF) at 1.95 MHz, a frequency used in mobile communication, affects the refolding kinetics of eukaryotic proteins (Mancinelli et al., 2004). On these basis we have evaluated the in vivo effect of MW-EMF in human epidermoid cancer KB cells. We have found t...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20327

    authors: Caraglia M,Marra M,Mancinelli F,D'Ambrosio G,Massa R,Giordano A,Budillon A,Abbruzzese A,Bismuto E

    更新日期:2005-08-01 00:00:00

  • Production of a colony-stimulating factor following differentiation of leukemic myleoblasts to macrophages.

    abstract::Leukemic cells in the myeloblastic stage from a murine myeloid leukemia cell line (M1) were induced to differentiate to macrophages by lipopolysaccharide (LPS) from Gram-negative bacteria. A granulocyte-macrophage colony-stimulating factor (CSF) was produced only during differentiation. After induction of differentiat...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041020307

    authors: Maeda M,Ichikawa Y

    更新日期:1980-03-01 00:00:00

  • Radiosensitivity enhancement by Co-NMS-mediated mitochondrial impairment in glioblastoma.

    abstract::We investigated the radiosensitizing effects of Co-NMS, a derivative of nimesulide based on a cobalt carbonyl complex, on malignant glioma cells. In the zebrafish exposed to Co-NMS ranging from 5 to 20 μM, cell death and heat shock protein 70 expression in the brain and neurobehavioral performance were evaluated. Our ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29774

    authors: Liu Y,Zhang T,Li G,Li S,Li J,Zhao Q,Wu Q,Xu D,Hu X,Zhang L,Li Q,Zhang H,Liu B

    更新日期:2020-12-01 00:00:00

  • Inflammatory molecules produced by meniscus and synovium in early and end-stage osteoarthritis: a coculture study.

    abstract::The aim of this study was to identify the molecules and pathways involved in the cross-talk between meniscus and synovium that may play a critical role in osteoarthritis (OA) pathophysiology. Samples of synovium and meniscus were collected from patients with early and end-stage OA and cultured alone or cocultured. Cyt...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27766

    authors: Favero M,Belluzzi E,Trisolino G,Goldring MB,Goldring SR,Cigolotti A,Pozzuoli A,Ruggieri P,Ramonda R,Grigolo B,Punzi L,Olivotto E

    更新日期:2019-07-01 00:00:00

  • Fucosylated umbilical cord blood hematopoietic stem cell expansion on selectin-coated scaffolds.

    abstract::Despite the advantages of transplantation of umbilical cord blood's (UCB's) hematopoietic stem cells (uHSCs) for hematologic malignancy treatment, there are two major challenges in using them: (a) Insufficient amount of uHSCs in a UCB unit; (b) a defect in uHSCs homing to bone marrow (BM) due to loose binding of their...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28825

    authors: Islami M,Payandeh Z,Dalir Abdolahinia E,Saburi E,Soleimanifar F,Kehtari M,Mortazavi Y,Nadri S,Darvish M

    更新日期:2019-12-01 00:00:00

  • Initiation of caspase-independent death in mouse mesangial cells by Cd2+: involvement of p38 kinase and CaMK-II.

    abstract::Cadmium (Cd) is a toxic metal with multiple effects on cell signaling and cell death. We studied the effects of Cd(2+) on quiescent mouse mesangial cells in serum-free conditions. Cadmium induces cell death over 6 h through annexin V+ states without or with causing uptake of propidium iodide, termed apoptotic and apop...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21499

    authors: Liu Y,Templeton DM

    更新日期:2008-11-01 00:00:00

  • A calcium requirement for movement of cultured cells.

    abstract::When calcium is removed from culture medium, motility of cultured cells is decreased. The effect is rapid, reversible and pronounced. Decreased motility is observed with normal mouse Balb/c 3T3 cells, mouse L929 cells, rat kidney fibroblasts and chick embryo fibroblasts. The calcium dependence of movement can be obser...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041010112

    authors: Moore L,Pastan I

    更新日期:1979-10-01 00:00:00

  • Inflammation Controls Sensitivity of Human and Mouse Intestinal Epithelial Cells to Galectin-1.

    abstract::Galectins play key roles in the inflammatory cascade. In this study, we aimed to analyze the effect of galectin-1 (Gal-1) in the function of intestinal epithelial cells (IECs) isolated from healthy and inflamed mucosa. IECs isolated from mice or patients with inflammatory bowel diseases (IBD) were incubated with diffe...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25249

    authors: Muglia CI,Gobbi RP,Smaldini P,Delgado ML,Candia M,Zanuzzi C,Sambuelli A,Rocca A,Toscano MA,Rabinovich GA,Docena GH

    更新日期:2016-07-01 00:00:00

  • L-ascorbic acid 2-phosphate stimulates collagen accumulation, cell proliferation, and formation of a three-dimensional tissuelike substance by skin fibroblasts.

    abstract::Proliferation of human skin fibroblasts was stimulated significantly by the presence of L-ascorbic acid 2-phosphate (Asc 2-P). The presence of Asc 2-P (0.1-1.0 mM) in the culture medium for 3 weeks enhanced the relative rate of collagen synthesis to total protein synthesis 2-fold as well as cell growth 4-fold. Coexist...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041380103

    authors: Hata R,Senoo H

    更新日期:1989-01-01 00:00:00

  • PBK promotes aggressive phenotypes of cervical cancer through ERK/c-Myc signaling pathway.

    abstract::Cervical cancer is the fourth most frequent cancer in women worldwide. PDZ-binding kinase (PBK) is proven to promote the malignant behaviors of various carcinomas. However, its functional roles and oncogenic mechanisms in cervical cancer are poorly understood. In this study, we reported that PBK was highly expressed i...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.30134

    authors: Ma H,Han F,Yan X,Qi G,Li Y,Li R,Yan S,Yuan C,Song K,Kong B

    更新日期:2020-11-13 00:00:00

  • Bafilomycin A1 inhibits lysosomal, phagosomal, and plasma membrane H(+)-ATPase and induces lysosomal enzyme secretion in macrophages.

    abstract::Bafilomycin A1, a specific inhibitor of H(+)-ATPases of the vacuolar type, was in the present study shown, at similar concentrations, to induce secretion of lysosomal enzyme and to elevate lysosomal pH in mouse macrophages. These results lend support to the previous suggestion of a triggering role for an increase in l...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041630116

    authors: Tapper H,Sundler R

    更新日期:1995-04-01 00:00:00

  • Contraction-induced interleukin-6 transcription in rat slow-type muscle is partly dependent on calcineurin activation.

    abstract::The present work aimed at determining whether interleukin-6 (IL-6) produced by skeletal muscle during exercise is related, at least partly, to calcineurin activity. Rats were treated with two specific calcineurin inhibitors, cyclosporin A (CsA) and FK506, or vehicle (Vhl); they were then subjected to exhaustive treadm...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20854

    authors: Banzet S,Koulmann N,Sanchez H,Serrurier B,Peinnequin A,Alonso A,Bigard X

    更新日期:2007-03-01 00:00:00

  • Zinc finger E-box binding homeobox 1 and atherosclerosis: New insights and therapeutic potential.

    abstract::Zinc finger E-box binding homeobox 1 (ZEB1), an important transcription factor belonging to the ZEB family, plays a crucial role in regulating gene expression required for both normal physiological and pathological processes. Accumulating evidence has shown that ZEB1 participates in the initiation and progression of a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.30177

    authors: Li H,Zou J,Yu XH,Ou X,Tang CK

    更新日期:2020-12-04 00:00:00

  • Elevation of angiotensin converting enzyme by 3-isobutyl-1-methylxanthine in cultured endothelial cells: a possible role for calmodulin.

    abstract::Incubation of cultured bovine pulmonary artery endothelial cells with 200 microM of 3-isobutyl-1-methylxanthine (IBMX) for 24 hr produced a five- to tenfold increase in cellular angiotensin converting enzyme activity (ACE) above that of untreated control cells. A lesser increase was observed in medium ACE. Other methy...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041370122

    authors: Dasarathy Y,Fanburg BL

    更新日期:1988-10-01 00:00:00

  • Nitric oxide and endothelin secretion by brain microvessel endothelial cells: regulation by cyclic nucleotides.

    abstract::Endothelin (ET)-1 was originally characterized as a potent vasoconstrictor peptide secreted by vascular endothelial cells. It possesses a wide range of biological activities within the cardiovascular system and in other organs, including the brain. Also secreted by endothelial cells, nitric oxide (NO), has recently be...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041550114

    authors: Durieu-Trautmann O,Fédérici C,Créminon C,Foignant-Chaverot N,Roux F,Claire M,Strosberg AD,Couraud PO

    更新日期:1993-04-01 00:00:00

  • Modulation of the development of human monocyte-derived dendritic cells by lithium chloride.

    abstract::Lithium has been used or explored to treat psychiatric and neurodegenerative diseases that are frequently associated with an abnormal immune status. It is likely that lithium may work through modulation of immune responses in these patients. Because dendritic cells (DC) play a central role in regulating immune respons...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22348

    authors: Liu KJ,Lee YL,Yang YY,Shih NY,Ho CC,Wu YC,Huang TS,Huang MC,Liu HC,Shen WW,Leu SJ

    更新日期:2011-02-01 00:00:00

  • Modulation of transforming growth factor-beta 1 stimulated elastin and collagen production and proliferation in porcine vascular smooth muscle cells and skin fibroblasts by basic fibroblast growth factor, transforming growth factor-alpha, and insulin-like

    abstract::During tissue repair and development, matrix accumulation is modulated as multiple signals impinge on target cells. We have investigated the effects of combinations of the mitogenic cytokines, basic fibroblast growth factor (bFGF), transforming growth factor alpha (TGF-alpha), and insulin-like growth factor-1 (IGF-1) ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041550119

    authors: Davidson JM,Zoia O,Liu JM

    更新日期:1993-04-01 00:00:00

  • Prognostic role of human equilibrative transporter 1 (hENT1) in patients with resected gastric cancer.

    abstract::Nucleoside transporter proteins are specialized proteins that mediate the transport of nucleosides and nucleoside analog drugs across the plasma membrane. The human equilibrative nucleoside transporter 1 (hENT1) is a member of these proteins and mediates cellular entry of gemcitabine, cytarabine, and fludarabine. The ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22045

    authors: Santini D,Vincenzi B,Fratto ME,Perrone G,Lai R,Catalano V,Cass C,Ruffini PA,Spoto C,Muretto P,Rizzo S,Muda AO,Mackey JR,Russo A,Tonini G,Graziano F

    更新日期:2010-05-01 00:00:00

  • Continuous maintenance of transformed fibroblasts under reduced serum conditions: utility as a model system for investigating growth factor-specific effects in nonquiescent cells.

    abstract::We report the continuous growth maintenance of untransformed and chemically transformed fibroblasts (AKR-2B, AKR-MCA cells) in low concentrations of serum (0.1% FBS). The cell lines established (AKR-0.1F, MCA-0.1F) proliferated at rates comparable to cells maintained under high serum conditions (10% FBS). Complete rem...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041380303

    authors: Mulder KM,Brattain MG

    更新日期:1989-03-01 00:00:00

  • Methylation of CLEC14A is associated with its expression and lung adenocarcinoma progression.

    abstract::Our main objective is probing the effect of methylation of CLEC14A on its expression and lung adenocarcinoma (LUAD) progression. Microarray analysis was utilized to screen out differentially downregulated genes with hypermethylation in LUAD tissues. The CLEC14A expression level was measured by western blot analysis an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27112

    authors: Su C,Shi K,Cheng X,Han Y,Li Y,Yu D,Liu Z

    更新日期:2019-03-01 00:00:00

  • TWEAK/Fn14 axis is an important player in fibrosis.

    abstract::Fibrosis is a common pathological condition associated with abnormal repair after tissue injury. However, the etiology and molecular mechanisms of fibrosis are still not well-understood. Tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) belongs to the TNF superfamily and acts by binding to its recepto...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.30089

    authors: Zhang Y,Zeng W,Xia Y

    更新日期:2020-09-30 00:00:00

  • Role of serum in the developmental expression of alkaline phosphatase in MC3T3-E1 osteoblasts.

    abstract::MC3T3-E1 cells in culture exhibit a temporal sequence of development similar to in vivo bone formation. To examine whether the developmental expression of the osteoblast phenotype depends on serum derived factors, we compared the time-dependent expression of alkaline phosphatase (ALP)-a marker of osteoblastic maturati...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041580311

    authors: Yohay DA,Zhang J,Thrailkill KM,Arthur JM,Quarles LD

    更新日期:1994-03-01 00:00:00

  • Metabolism and regulation of gene expression by 4-oxoretinol versus all-trans retinoic acid in normal human mammary epithelial cells.

    abstract::We previously demonstrated that 4-oxoretinol (4-oxo-ROL) activated retinoic acid receptors (RARs) in F9 stem cells. We showed that 4-oxo-ROL inhibited the proliferation of normal human mammary epithelial cells (HMECs). To understand the mechanisms by which 4-oxo-ROL regulates HMEC growth we examined gene expression pr...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21824

    authors: Liu L,Derguini F,Gudas LJ

    更新日期:2009-09-01 00:00:00

  • Interleukin-6 and transforming growth factor-beta synergistically stimulate chondrosarcoma cell proliferation.

    abstract::This study examines the regulation of Swarm rat chondrosarcoma (SRC) cell proliferation in vitro. In serum-free cultures, SRC cells showed only transient DNA synthesis and this was increased by serum. Transforming growth factor-beta (TGF-beta) was identified as an essential serum component, since the mitogenic effect ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041490115

    authors: Guerne PA,Lotz M

    更新日期:1991-10-01 00:00:00

  • Antagonistic effects of insulin and dexamethasone on glucose-regulated and heat shock protein synthesis.

    abstract::The present study extends our previous observation (Kasambalides and Lanks, J. Cell. Physiol., 114:93-98, 1983), that dexamethasone inhibits the alterations in heat shock protein (HSP) and glucose-regulated protein (GRP) synthesis caused by glucose deprivation. We now show that insulin, even in the presence of high ex...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041230220

    authors: Kasambalides EJ,Lanks KW

    更新日期:1985-05-01 00:00:00

  • Frequency-dependent cell death by optical tweezers manipulation.

    abstract::Optical tweezers were used to scan individual Chronic Myelogenous Leukemia cells to determine if the cell death depends on the scanning conditions. Although increasing the scanning frequency or amplitude means greater force applied to the cells, their effects on cell death are not a simple increasing trend, as observe...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24369

    authors: Ng KS,Zhou ZL,Ngan AH

    更新日期:2013-10-01 00:00:00

  • Effects of interaction between estradiol-17 beta and progesterone on the proliferation of cloned breast tumor cells (MCF-7 and T47D).

    abstract::We have previously reported that the proliferation of cloned MCF-7 and T47D human mammary tumor cells can be inhibited by increasing concentrations of charcoal-dextran stripped female human serum (CDFHS). The maximal proliferation rate was restored by the addition of 3 X 10(-11) M estradiol-17 beta to the culture medi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041240305

    authors: Schatz RW,Soto AM,Sonnenschein C

    更新日期:1985-09-01 00:00:00

  • Upregulation of amphiregulin by retinoic acid and Wnt signalling promotes liver cancer cell proliferation.

    abstract::Activated hepatic stellate cells promote hepatocellular carcinoma (HCC) progression. Hepatic stellate cells play a key role in retinoid metabolism, and activation of stellate cells increases retinoic acid (RA) in the liver. However, the role of RA in HCC proliferation remains unclear. We aimed to analyse the mechanism...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29088

    authors: Lin HH,Peng YJ,Tsai MJ,Wu YY,Tsai TN,Huang HH,Shih YL,Chang WK,Hsieh TY

    更新日期:2020-02-01 00:00:00

  • Induction of HIF-1alpha expression by intermittent hypoxia: involvement of NADPH oxidase, Ca2+ signaling, prolyl hydroxylases, and mTOR.

    abstract::Sleep-disordered breathing with recurrent apnea (periodic cessation of breathing) results in chronic intermittent hypoxia (IH), which leads to cardiovascular and respiratory pathology. Molecular mechanisms underlying IH-evoked cardio-respiratory co-morbidities have not been delineated. Mice with heterozygous deficienc...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21537

    authors: Yuan G,Nanduri J,Khan S,Semenza GL,Prabhakar NR

    更新日期:2008-12-01 00:00:00