Oxygen-induced transcriptional dynamics in human osteoblasts are most prominent at the onset of mineralization.


:Oxygen tension plays an important role in the regulation of cellular processes. During hematopoietic stem cell (HSC) differentiation, HSCs migrate from one stem cell niche to the next, each with a different oxygen tension that determines which signaling pathways are on and off, determining the differentiation stage of the cell. Oxygen tension influences osteoblast differentiation and mineralization. Low oxygen levels inhibit matrix formation and mineralization. We were interested in the regulatory mechanisms that underlie this inhibition and wondered whether a switch in oxygen tension could have varying effects depending on the differentiation phase of the osteoblasts. We performed an oxygen tension switch phase study in which we switched osteoblasts from high to low oxygen tension during their 3 week differentiation and mineralization process. We performed microarray expression profiling on samples collected during this 3 week period and analyzed biochemical and histo-chemical endpoint parameters to determine the effect of a switch in oxygen levels on mineralization. We found that low oxygen tension has the most profound impact on mineralization when administered during the period of matrix maturation. Additionally, a large set of genes was regulated by oxygen, independent of the differentiation phase. These genes were involved in cell metabolisms and matrix formation. Our study demonstrates that variation in oxygen tension strongly affects gene expression in differentiating osteoblasts. The magnitude of this change for either expression levels or the number of regulated probes, depends on the osteoblast differentiation stage, with the phase prior to the onset of mineralization being most sensitive.


J Cell Physiol


Nicolaije C,van de Peppel J,van Leeuwen JP




Has Abstract


2013-09-01 00:00:00












  • Growth-dependent subcellular redistribution of protein kinase C in cultured porcine aortic endothelial cells.

    abstract::We have previously observed major differences in the phosphorylation of membrane proteins in sparse, proliferating versus confluent, quiescent pig aortic endothelial cells (EC) (Kazlauskas and DiCorleto, 1987). In the present study we examined whether EC growth state can influence the activity of a specific phosphoryl...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Uratsuji Y,DiCorleto PE

    更新日期:1988-09-01 00:00:00

  • Epidermal growth factor receptor-dependent PI3K-activation promotes restitution of wounded human gastric epithelial monolayers.

    abstract::Restitution is a crucial event during the healing of superficial injury of the gastric mucosa involving epithelial cell sheet movement into the damaged area. We demonstrated that growth factors promote the restitution of human gastric epithelial cells. However, the intracellular signaling pathways that transmit extrac...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tétreault MP,Chailler P,Beaulieu JF,Rivard N,Ménard D

    更新日期:2008-02-01 00:00:00

  • Transforming growth factor beta responsiveness is modulated by the extracellular collagen matrix during hepatic ito cell culture.

    abstract::Hepatic sinusoidal Ito cells have the capacity to produce interstitial collagen types I and III as well as other matrix proteins and may be involved in hepatic fibrogenesis. Transforming growth factor beta (TGF beta) responsiveness was evaluated during in vitro cell culture, since increasing evidence suggests that thi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Davis BH

    更新日期:1988-09-01 00:00:00

  • CRM197 in Combination With shRNA Interference of VCAM-1 Displays Enhanced Inhibitory Effects on Human Glioblastoma Cells.

    abstract::CRM197 is a naturally nontoxic diphtheria toxin mutant that binds and inhibits heparin-binding epidermal growth factor-like growth factor. CRM197 serves as carrier protein for vaccine and other therapeutic agents. CRM197 also inhibits the growth, migration, invasion, and induces apoptosis in various tumors. Vascular c...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hu Y,Lin X,Wang P,Xue YX,Li Z,Liu LB,Yu B,Feng TD,Liu YH

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    abstract::The p21 activated kinases (Paks), an evolutionarily conserved family of serine/threonine kinases, are important for a variety of cellular functions including cell morphogenesis, motility, survival, mitosis, and angiogenesis. Paks are widely expressed in numerous tissues and are activated by growth factors and extracel...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Kumar R,Vadlamudi RK

    更新日期:2002-11-01 00:00:00

  • Selenium deficiency in cultured adrenocortical cells: restoration of glutathione peroxidase and resistance to hydroperoxides on addition of selenium.

    abstract::Cultured bovine adrenocortical cells were previously shown to be functionally deficient in selenium and vitamin E when grown in medium supplemented with fetal bovine serum. In the present experiments, the lack of significant bioavailable amounts of selenium in the medium was demonstrated by the finding of only low lev...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hornsby PJ,Pearson DW,Autor AP,Aldern KA,Harris SE

    更新日期:1985-04-01 00:00:00

  • Cover Image, Volume 232, Number 12, December 2017.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Reagan MR,Lian JB,Rosen CJ,Stein GS

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  • ADP-Induced Ca2+ Signaling and Proliferation of Rat Ventricular Myofibroblasts Depend on Phospholipase C-Linked TRP Channels Activation Within Lipid Rafts.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Certal M,Vinhas A,Barros-Barbosa A,Ferreirinha F,Costa MA,Correia-de-Sá P

    更新日期:2017-06-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Horn PL,Turker MS,Ogburn CE,Disteche CM,Martin GM

    更新日期:1984-11-01 00:00:00

  • FUS-induced circular RNA ZNF609 promotes tumorigenesis and progression via sponging miR-142-3p in lung cancer.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Liu S,Yang N,Jiang X,Wang J,Dong J,Gao Y

    更新日期:2021-01-01 00:00:00

  • Ferritin light chain down-modulation generates depigmentation in human metastatic melanoma cells by influencing tyrosinase maturation.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Maresca V,Flori E,Cardinali G,Briganti S,Lombardi D,Mileo AM,Paggi MG,Picardo M

    更新日期:2006-03-01 00:00:00

  • EMAST status as a beneficial predictor of fluorouracil-based adjuvant chemotherapy for Stage II/III colorectal cancer.

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    journal_title:Journal of cellular physiology

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    authors: Mohammadpour S,Goodarzi HR,Jafarinia M,Porhoseingholi MA,Nazemalhosseini-Mojarad E

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mirzapoiazova T,Kolosova IA,Romer L,Garcia JG,Verin AD

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  • Transcription of the pain-related TRPV1 gene requires Runx1 and C/EBPβ factors.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ugarte GD,Diaz E,Biscaia M,Stehberg J,Montecino M,van Zundert B

    更新日期:2013-04-01 00:00:00

  • LIGHT regulates CD86 expression on dendritic cells through NF-kappaB, but not JNK/AP-1 signal transduction pathway.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zou GM,Hu WY

    更新日期:2005-12-01 00:00:00

  • Transglutaminase activity regulates differentiation, migration and fusion of osteoclasts via affecting actin dynamics.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sun H,Kaartinen MT

    更新日期:2018-09-01 00:00:00

  • Apoptosis of human erythroid colony-forming cells is decreased by stem cell factor and insulin-like growth factor I as well as erythropoietin.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Muta K,Krantz SB

    更新日期:1993-08-01 00:00:00

  • Follistatin-like protein 1 induction of matrix metalloproteinase 1, 3 and 13 gene expression in rheumatoid arthritis synoviocytes requires MAPK, JAK/STAT3 and NF-κB pathways.

    abstract::Elevated levels of follistatin-like protein 1 (FSTL1) have been found both in mouse models for human rheumatoid arthritis (RA) and collagen-induced arthritis (CIA). In this study, we elucidated the potential mechanisms by which FSTL1 contributes to the pathogenesis of RA. Fibroblast-like synoviocytes (FLSs) were estab...

    journal_title:Journal of cellular physiology

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    authors: Ni S,Li C,Xu N,Liu X,Wang W,Chen W,Wang Y,van Wijnen AJ

    更新日期:2018-01-01 00:00:00

  • Leukemia stem cell immunophenotyping tool for diagnostic, prognosis, and therapeutics.

    abstract::The existence of cancer stem cells is debatable in numerous solid tumors, yet in leukemia, there is compelling evidence of this cell population. Leukemic stem cells (LSCs) are altered cells in which accumulating genetic and/or epigenetic alterations occur, resulting in the transition between the normal, preleukemic, a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Mastelaro de Rezende M,Ferreira AT,Paredes-Gamero EJ

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Beland JL,Yuille MA,Hugunin M,Zhang XM,Silverstone AE

    更新日期:1990-03-01 00:00:00

  • Long noncoding RNA HOXC13-AS positively affects cell proliferation and invasion in nasopharyngeal carcinoma via modulating miR-383-3p/HMGA2 axis.

    abstract::Long noncoding RNAs have been reported to be important regulators in numerous cancers. In this study, we found that HOXC13 antisense RNA (HOXC13-AS) was highly expressed in head and neck squamous carcinoma (HNSC) tissues in The Cancer Genome Atlas database. Nasopharyngeal carcinoma (NPC) belongs to HNSC. Therefore, we...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Gao C,Lu W,Lou W,Wang L,Xu Q

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  • Growth inhibitory activity in extracts from human fibroblasts.

    abstract::Indirect evidence for the presence of a growth inhibitor in normal human fibroblasts has been obtained previously; the inhibitory activity has been found associated with crude cell extracts, but the molecule responsible for the growth inhibition has never been isolated. We have isolated a glycopeptide fraction from hu...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Macieira-Coelho A,Söderberg A

    更新日期:1993-01-01 00:00:00

  • Epidermal growth factor treatment of A431 cells alters the binding capacity and electrophoretic mobility of the cytoskeletally associated epidermal growth factor receptor.

    abstract::The epidermal growth factor (EGF) receptor interacts with structural elements of A431 cells and remains associated with the cytoskeleton following extraction with nonionic detergents. Extraction of cells with 0.15% Triton X-100 resulted in detection of only approximately 40% of the EGF binding sites on the cytoskeleto...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Roy LM,Gittinger CK,Landreth GE

    更新日期:1991-01-01 00:00:00

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    abstract::During interphase, the centrosome concentrates cell stress response molecules, including chaperones and proteasomes, into a proteolytic center. However, whether the centrosome functions as proteolytic center during mitosis is not known. In this study, cultured mammalian cells were treated with the proteasome inhibitor...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ehrhardt AG,Sluder G

    更新日期:2005-09-01 00:00:00

  • Antagonistic effects of insulin and dexamethasone on glucose-regulated and heat shock protein synthesis.

    abstract::The present study extends our previous observation (Kasambalides and Lanks, J. Cell. Physiol., 114:93-98, 1983), that dexamethasone inhibits the alterations in heat shock protein (HSP) and glucose-regulated protein (GRP) synthesis caused by glucose deprivation. We now show that insulin, even in the presence of high ex...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kasambalides EJ,Lanks KW

    更新日期:1985-05-01 00:00:00

  • 24,25-(OH)2D3 regulates protein kinase C through two distinct phospholipid-dependent mechanisms.

    abstract::We have previously shown that 24,25-(OH)2D3 plays a major role in resting zone (RC) chondrocyte differentiation and that this vitamin D metabolite regulates protein kinase C (PKC). The aim of the present study was to identify the signal transduction pathway used by 24,25-(OH)2D3 to stimulate PKC activation. Confluent,...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Helm S,Sylvia VL,Harmon T,Dean DD,Boyan BD,Schwartz Z

    更新日期:1996-12-01 00:00:00

  • The effects of Clostridium perfringens enterotoxin on morphology, viability, and macromolecular synthesis in Vero cells.

    abstract::Vero (African green monkey kidney) cells grown in tissue culture monolayer were sensitive to Clostridium perfringens enterotoxin. Within 30 minutes of exposure to the enterotoxin gross morphological damage was observed and within 40 minutes approximately 75% of the cells had detached. Nearly half of the cells were non...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: McClane BA,McDonel JL

    更新日期:1979-05-01 00:00:00

  • Role of insulin-like growth factors and their binding proteins in growth control and carcinogenesis.

    abstract::Interest in the role of the insulin-like growth factor (IGF) axis in growth control and carcinogenesis has recently been increased by the finding of elevated serum insulin-like growth factor I (IGF-I) levels in association with three of the most prevalent cancers in the United States: prostate cancer, colorectal cance...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Grimberg A,Cohen P

    更新日期:2000-04-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Feneant M,Moatti N,Lemonnier F,Lemonnier A

    更新日期:1981-10-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dai JM,Wang ZY,Sun DC,Lin RX,Wang SQ

    更新日期:2007-01-01 00:00:00