Abstract:
:The purpose of this study was to characterize the direct effects of uncarboxylated osteocalcin (ucOCN) on vascular cell biology in vitro, to assess its potential function in pathophysiological conditions such as atherosclerosis. Human aortic endothelial cells (HAECs) and smooth muscle cells (HASMCs) were treated with ucOCN (0.1-50 ng/ml) and changes in phosphorylation of intracellular signaling proteins, angiogenesis, proliferation, migration, monolayer permeability, and protein secretion were measured. In HAECs, phosphorylated JNK and CREB were decreased with ucOCN (p < 0.05). In HASMCs, phosphorylated p70S6K and NF-ΚB were increased by ucOCN (p < 0.05). Cell proliferation increased in both cell types dose dependently which was blocked by AKT and ERK pathway inhibitors. ucOCN did not affect cell permeability, angiogenesis, or migration. The direct activity of ucOCN on vascular cells is recognized, particularly its proliferative effects. However, at least in physiological settings, it does not appear that osteocalcin may directly promote atherogenesis based on the outcomes measured.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Millar SA,Anderson SI,O'sullivan SEdoi
10.1002/jcp.28707subject
Has Abstractpub_date
2019-11-01 00:00:00pages
21039-21048issue
11eissn
0021-9541issn
1097-4652journal_volume
234pub_type
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journal_title:Journal of cellular physiology
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doi:10.1002/jcp.1041620205
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