Abstract:
:Very little is known about the effects of pressure within the vascular system on EC phenotype. To study this, bovine aortic EC were seeded on rat type I collagen plates (2,000/cm2) and allowed to attach for 24 hours. The cells were exposed to either atmospheric, 40, 80, or 120 mm Hg pressure by placing them in a plexiglass pressure chamber loaded with 5% CO2/air and maintained at 37 degrees C inside an incubator. Chamber pressure was continuously monitored with an amplified voltage transducer connected to a digital monitor. EC were maintained in DMEM supplemented with 10% calf serum and substrates for up to 9 days. The results indicate that EC proliferation is influenced by their ambient pressure. EC subjected in vitro to pressures comparable to mean systemic blood pressures had a significant increase in cell number compared to EC exposed to atmospheric pressures. EC were elongated and appeared to align randomly. We hypothesize that the systemic pressure which the endothelium is exposed to in vivo may have a significant regulatory influence on the ability of the endothelium to proliferate which may affect the endothelial cell response to injury.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Sumpio BE,Widmann MD,Ricotta J,Awolesi MA,Watase Mdoi
10.1002/jcp.1041580117subject
Has Abstractpub_date
1994-01-01 00:00:00pages
133-9issue
1eissn
0021-9541issn
1097-4652journal_volume
158pub_type
杂志文章abstract::The effect of epinephrine was tested on the proliferation of rat arterial smooth muscle cells (SMC) in secondary cultures. Epinephrine added daily to the culture medium caused a striking stimulation of growth. The effect increased with time and was dose-dependent. Maximal stimulation was observed at a concentration of...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041160207
更新日期:1983-08-01 00:00:00
abstract:OBJECTIVE:To investigate whether mature human articular chondrocytes (AC) exhibit an antiproliferative effect on activated peripheral blood mononuclear cells (PBMC) and to compare this effect with other cells of mesenchymal origin. METHODS:AC from healthy cadaveric cartilage were grown for different passages, in the a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20789
更新日期:2006-12-01 00:00:00
abstract::Thymosin β4 (Tβ4), a G-actin-sequestering secreted peptide, improves neurovascular remodeling and central nervous system plasticity, which leads to neurological recovery in many neurological diseases. Inflammatory response adjustment and tissue inflammation consequences from neurological injury are vital for neurologi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.29293
更新日期:2020-04-01 00:00:00
abstract::A proliferation inducing ligand (APRIL or TALL-2 and TRDL-1) was first discovered as a cytokine over-expressed in many transformed cells and with the capacity to stimulate proliferation. APRIL was shown to bind two different receptors of the TNF receptor superfamily: B cell maturation antigen (BCMA) and transmembrane ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.21561
更新日期:2009-01-01 00:00:00
abstract::The tumor microenvironment significantly affects tumor progression, and tumor cells can also remodel the tumor microenvironment through complex interaction. Inflammasomes are innate immune system receptors/sensors that regulate an inflammatory response mainly mediated by the nucleotide-binding oligomerization domain-l...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30169
更新日期:2020-11-23 00:00:00
abstract::The effects of three purified colony-stimulating factors (CSFs) with different specificities for the granulocyte (G) and macrophage (M) lineages (G-CSF, CSF-1 and GM-CSF) were studied in a serum-free clonal assay system. The results were compared with those obtained in similar cultures containing fetal calf serum (FCS...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041280214
更新日期:1986-08-01 00:00:00
abstract::Ascorbic acid induces apoptosis, autophagy, and necrotic cell death in cancer cells. We investigated the mechanisms by which ascorbic acid induces death in laryngeal squamous cell carcinoma Hep2 cells. Ascorbic acid markedly reduced cell viability and induced death without caspase activation and an increase in cytochr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25438
更新日期:2017-02-01 00:00:00
abstract::Baculoviral IAP repeat containing 5 (BIRC5) gene encodes the important protein as survivin, a multifunctional protein, which is involved in cellular and molecular networks, progression of cell cycle, homeostasis, developmental morphogenesis, and apoptosis. The proximal BIRC5 promoter possesses specific binding sites f...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28725
更新日期:2019-11-01 00:00:00
abstract::Hyperthermia is a clinical sign of inflammation and constitutes in itself an adaptive defense mechanism. The fibrinolytic system, a highly regulated proteolytic system, is involved in inflammatory processes. Plasminogen activator inhibitor 1 (PAI-1) is the principal inhibitor of the two activators of the fibrinolytic ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041480217
更新日期:1991-08-01 00:00:00
abstract::The experiments reported were motivated by the observation that in vivo gangliosides promoted angiogenesis when the dose of the angiogenic factor was too low to be effective (Ziche et al.: Laboratory Investigation 61:629-634, 1989). As an approach to understanding the mechanism of this modulatory effect, we analysed t...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041440319
更新日期:1990-09-01 00:00:00
abstract::We have partially purified a mitogenic factor, termed colony stimulating factor, which is secreted from a human T lymphocytic cell line, and which stimulates in vitro formation of colonies of macrophages and granulocytes by human bone marrow cells. Colony stimulating factor (CSF) produced by cells grown in serum suppl...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041090309
更新日期:1981-12-01 00:00:00
abstract::Studies on a normal human diploid cell strain revealed that the specific activity of the cell protein, for each of the three enzymes of the Leloir pathway, changed significantly as the cells grew. The kinetics of change in specific activity varied according to the enzyme being studied, and the kinetics for each enzyme...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040860112
更新日期:1975-08-01 00:00:00
abstract::The development of resistance accounts for therapy failure in the majority of advanced cases of neuroblastoma in children. A new transplantable murine C-1300 neuroblastoma cell line was developed in vitro, by repeated exposure of a sensitive cell line to increasing, but sublethal, doses of Homoharringtonine (HHT). The...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041480319
更新日期:1991-09-01 00:00:00
abstract::We can distinguish two classes of membrane transport changes in cultured cells: (a) growth-rate contingent changes are those which occur in coordination with the onset of density-dependent inhibition of growth; (b) transformation-specific changes are those which occur when cells become transformed, and which can be de...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040890431
更新日期:1976-12-01 00:00:00
abstract::Cytoskeleton not only controls cell morphology but also regulates cell growth, migration, differentiation, and gene expression, events which are fundamental to embryogenesis, carcinogenesis, and wound healing. We have recently reported that reorganization of cytoskeleton induces expression of mRNA for transforming gro...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199708)172:2<192::AID-JCP6
更新日期:1997-08-01 00:00:00
abstract::Nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) is a subtype of the NOX family, which is mainly expressed in the pulmonary vasculature and pulmonary endothelial cells in the respiratory system. NOX4 has unique characteristics, and is a constitutively active enzyme that primarily produces hydrogen peroxide...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.30005
更新日期:2021-03-01 00:00:00
abstract::The vascular isoform of ATP-sensitive K(+) (KATP ) channels regulates blood flow to all organs. The KATP channel is strongly inhibited by reactive oxygen and carbonyl species produced in diabetic tissue inflammation. To address how such channel inhibition impacts vascular regulation as well as tissue viability, we per...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25003
更新日期:2015-12-01 00:00:00
abstract::Myeloid cells, including granulocytes and monocyte/macrophages, are important in disease-associated inflammatory reactions. These cells come from a common progenitor, the promyelocyte. The human promyelocytic cell line, HL-60, can be induced to terminally differentiate into granulocytes or monocyte/ macrophages in a c...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199607)168:1<155::AID-JCP1
更新日期:1996-07-01 00:00:00
abstract::Fibrosis is a common pathological condition associated with abnormal repair after tissue injury. However, the etiology and molecular mechanisms of fibrosis are still not well-understood. Tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) belongs to the TNF superfamily and acts by binding to its recepto...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.30089
更新日期:2020-09-30 00:00:00
abstract::MicroRNAs (miRNAs or miRs) are small noncoding RNAs that play critical roles in muscle cell proliferation and differentiation via post-transcriptional regulation of gene expression. Here, based on our previous high-throughput sequencing results, we evaluated miRNA-499 (miR-499) functions during myoblast proliferation ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26903
更新日期:2019-03-01 00:00:00
abstract::Serine/threonine protein phosphatase (PP) 2A regulates many biological processes, however it remains unclear whether PP2A participates in cadherin-mediated cell-cell adhesion. We show here that the core enzyme of PP2A (PP2A-AC) is localized in the cell-cell adhesion sites between adjacent cells and associated with the...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20524
更新日期:2006-03-01 00:00:00
abstract::Hydatid cyst, the larval stage of the tapeworm Echinococcus granulosus and a causative agent of cystic echinococcosis, possesses a vast number of antigenic peptides that are constantly presented in the host immune system during infection. Here, we sought to provide more information about the cellular/humoral component...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27092
更新日期:2019-03-01 00:00:00
abstract::The concentration of ATP in cysts of the brine shrimp, Artemia salina, has been studied as a function of cyst hydration. Cysts dried over CaSO4 contain 0.02 gH2O/g cysts and 0.54 +/- 0.05 (S.D.) mumoles of ATP/g dried cysts. Addition of water up to 0.05 g/g cysts produced no net change in the level of ATP during incub...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040880205
更新日期:1976-06-01 00:00:00
abstract::Cells of confluent cultures of the established pig renal epithelial line, LLC-PK1, accumulate alpha-methyl-D-glucoside against a concentration gradient. This transport system is strongly inhibited by phlorizin and 6-deoxy-D-glucose, moderately inhibited by phloretin, and only weakly inhibited by 3-0-methyl-D-glucose, ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041040311
更新日期:1980-09-01 00:00:00
abstract::Active oxygen species are generated in cells during pathophysiologic conditions such as inflammation and postischemic reperfusion. If oxygen radical scavengers are added before reperfusion, then the magnitude of injury is reduced. We investigated whether free radicals generated following exposure to hypoxia and reoxyg...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041560217
更新日期:1993-08-01 00:00:00
abstract::The present study was performed to clarify second messenger signaling in parathyroid hormone (PTH)-induced c-fos gene expression, to characterize the participation of the c-fos gene in the regulation of osteoblast proliferation and function as well as osteoclast-like cell formation by PTH and to compare these effects ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041610221
更新日期:1994-11-01 00:00:00
abstract::Inhibition of the gap-filling, polymerizing step of excision repair by 1-beta-D-arabinofuranosylcytosine (ara-C) after irradiation with ultraviolet light in human diploid fibroblasts resulted in the formation of persistent DNA strand breaks in G1, G2, and plateau phase cells, but not in S phase cells. Addition of hydr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041080207
更新日期:1981-08-01 00:00:00
abstract::The effects of kinin B1 receptor (B1 R) deletion were examined on femur bone regeneration in streptozotocin (STZ)-type 1 diabetes. Diabetes induction in wild-type C57/BL6 (WTC57BL6) mice led to decrease in body weight and hyperglycemia, compared to the non-diabetic group of the same strain. The lack of B1 R did not af...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25034
更新日期:2015-12-01 00:00:00
abstract::Norepinephrine (NE) produced a dose-dependent inhibition of 125I-epidermal growth factor (EGF) binding to adult rat hepatocytes in primary culture. This effect was maximal after 1 hr of incubation with NE and could be blocked by the presence of an alpha 1-specific adrenergic receptor antagonist. The inhibition of bind...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041270106
更新日期:1986-04-01 00:00:00
abstract::We have examined the transport of amino acids by the sodium-dependent "A" and "ASC" system in thymic- and splenic-derived lymphocytes from the Long-Evans rat. Lymphocytes derived from the thymus transport amino acids by both the "A" and "ASC" systems, whereas lymphocytes from the spleen transport amino acids by the "A...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040970205
更新日期:1978-11-01 00:00:00