Abstract:
:The carrier of uridine transport in hamster cells in culture is highly susceptible to the inhibitory effect of probes like S-benzylated derivatives of mercaptopurine nucleosides. The interaction between the probes and the carrier is competitive and reversible and it takes place at a site different from the substrate binding site. The Ki for the most potent derivative p-nitrobenzyl-6-mercaptoinosine is 0.15 n Molar at 20 degrees C. The effect of the probes is interpreted in terms of conformational change induced on the carrier upon binding of the probe. The carrier assumes distinct conformations depending on whether it is probe-free (form A) or probe bound (form B). Kinetic as well as chemical evidence supports the predictions of the allosteric carrier model. A single component of kinetics is observed either in the absence of inhibitor (Km form A) or at high concentrations of inhibitor (Km form B). A two component kinetics is observed at intermediate concentrations of inhibitor (some carriers in form B and others in form A). The two forms have distinct Km values for uridine: form A50 muMolar and form B 250 muMolar. Two forms have also different susceptibilities to the action of organomercurials: form A is insensitive whereas form B is highly inhibited by the chemical modified of SH groups. The existence of putative allosteric sites in carriers is discussed in terms of modifier sites capable of modulating transport activities as a result of specific membrane-ligand interactions.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Eilam Y,Carbantchik Idoi
10.1002/jcp.1040890451keywords:
subject
Has Abstractpub_date
1976-12-01 00:00:00pages
831-8issue
4eissn
0021-9541issn
1097-4652journal_volume
89pub_type
杂志文章abstract::Two transport systems for neutral amino acids have been characterised in LLC-PK, cells. The first, which transports alanine in a sodium-dependent manner, also mediates alanine exchange and is perferentially inhibited by serine, cysteine, and alpha-amino-n-butyric acid. This system resembles the ASC system in Ehrlich a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041120205
更新日期:1982-08-01 00:00:00
abstract::The ratio of mouse to rat albumin secreted by mouse hepatoma x rat hepatoma hybrid cells is constant (of the order of 5.0) irrespective of the total amounts produced. The present results establish for seven independent hybrid clones that the coordination in the ratio of mouse to rat product applies also at the level o...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041150211
更新日期:1983-05-01 00:00:00
abstract::During interphase, the centrosome concentrates cell stress response molecules, including chaperones and proteasomes, into a proteolytic center. However, whether the centrosome functions as proteolytic center during mitosis is not known. In this study, cultured mammalian cells were treated with the proteasome inhibitor...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20335
更新日期:2005-09-01 00:00:00
abstract::We have shown in the present study and in studies reported previously that preneoplastic and neoplastic rat tracheal epithelial (RTE) cell lines express TGF alpha and do so regardless of the mechanism by which they were transformed. In order to determine whether TGF alpha is an autocrine growth regulator of immortaliz...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041470121
更新日期:1991-04-01 00:00:00
abstract::The polyphenolic compounds curcumin and quercetin increased sensitivity of ovarian cancer cells (CAOV3 and SKOV3) to cisplatin. The effect was obtained when the compounds were added simultaneously with cisplatin, as well as when they were added 24 h before. High serum levels of certain cytokines, for example interleuk...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10186
更新日期:2003-01-01 00:00:00
abstract::Lipocalin-2 (Lcn2; also termed neutrophil gelatinase-associated lipocalin (NGAL)) levels correlate positively with heart failure (HF) yet mechanisms via which Lcn2 contributes to the pathogenesis of HF remain unclear. In this study, we used coronary artery ligation surgery to induce ischemia in wild-type (wt) mice and...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25672
更新日期:2017-08-01 00:00:00
abstract::Exposure to D-allose has been demonstrated to lead to decreased 2-deoxy-D-glucose (2-DG) and 3-0-methyl-D-glucose transport in the V79 Chinese hamster lung fibroblast cell line. The effect of D-allose 1) was maximal after 4 hours exposure to the cells; 2) was optimal between 2.77 and 5.55 mM D-allose; and 3) led to a ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041450217
更新日期:1990-11-01 00:00:00
abstract::Embryo implantation is essential for normal pregnancy, and the process of decidualization is critical for embryo implantation. However, the mechanism of decidualization during early pregnancy is still unknown. Forkhead box O3a (FOXO3a) is the most important functional transcription factor of the forkhead box family an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27167
更新日期:2019-04-01 00:00:00
abstract::Alternative splicing (AS) is critically associated with tumorigenesis and patient's prognosis. Here, we systematically analyzed survival-associated AS signatures in oral squamous cell carcinoma (OSCC) and evaluated their prognostic predictive values. Survival-related AS events were identified by univariate and multiva...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29357
更新日期:2020-05-01 00:00:00
abstract::Rous sarcoma virus (RSV)-infected chicken embryo cells were used to study the effect of viral transformation on the hormone-stimulated synthesis of cyclic AMP. Transformation by RSV greatly increased the cells' ability to synthesize and accumulate cyclic AMP in response to the beta-adrenergic agonist isoproterenol as ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041110311
更新日期:1982-06-01 00:00:00
abstract::Lung cancer is a leading cause of cancer-related death worldwide. In this study, we used lung adenocarcinoma cells as a model, as lung adenocarcinoma has the highest mortality rate among all lung cancers. For the past few years, medical treatments or lung cancer have been limited because of chemotherapy resistance. Th...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30133
更新日期:2020-12-07 00:00:00
abstract::Beta-arrestins are scaffolding proteins implicated as negative regulators of TLR4 signaling in macrophages and fibroblasts. Unexpectedly, we found that beta-arrestin-1 (beta-arr-1) and -2 knockout (KO) mice are protected from TLR4-mediated endotoxic shock and lethality. To identify the potential mechanisms involved, w...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22289
更新日期:2010-11-01 00:00:00
abstract::We have utilized the in situ perfused rat liver under nonrecirculating conditions to examine the effect of temperature on the metabolism and biliary secretion of [125I]-asialoorosomucid (ASOR). In this manner we were able to follow the fate of a single round of internalized ligand. In control livers perfused at 37 deg...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041380316
更新日期:1989-03-01 00:00:00
abstract::The extent to which autolytic microbial enzymes are involved in the fate of microorganisms ingested by phagocytes has not been determined. It is known, however, that activation of degradative enzymes occurs during certain microbicidal events. We examined the possible role of the pneumococcal autolytic enzyme (an N-ace...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040920203
更新日期:1977-08-01 00:00:00
abstract::The present work aimed at determining whether interleukin-6 (IL-6) produced by skeletal muscle during exercise is related, at least partly, to calcineurin activity. Rats were treated with two specific calcineurin inhibitors, cyclosporin A (CsA) and FK506, or vehicle (Vhl); they were then subjected to exhaustive treadm...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20854
更新日期:2007-03-01 00:00:00
abstract::Nucleotide receptors signaling affects cell proliferation, with possible implications on tumorigenic processes. However, molecular targets and action mechanisms of the extracellular nucleotides are still poorly elucidated. We have previously shown in ARO cells that APE1/Ref-1, a transcriptional coactivator responsible...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20704
更新日期:2006-10-01 00:00:00
abstract::The effects of pulsatile and steady fluid flow on the mRNA levels of proto-oncogenes c-fos, c-jun, and c-myc in cultured human umbilical vein endothelial cells (HUVEC) were investigated. c-fos mRNA levels in stationary cultures were very low. A 1 Hz pulsatile flow with an average shear stress of 16 dynes/cm2 induced a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041540118
更新日期:1993-01-01 00:00:00
abstract::When human diploid fibroblasts IMR-90 are cultured in routinely used medium (Eagle's basal medium supplemented with 10% fetal calf serum), sulfhydryl compounds appear in the medium. The major component of these sulfhydryl compounds is cysteine, and it is shown that a part of medium cystine is converted into cysteine b...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041040211
更新日期:1980-08-01 00:00:00
abstract::The SV40 transformed murine macrophage cell line, BAC1, proliferates in response to the colony stimulating factor, CSF-1 (Schwarzbaum et al., J. Immunol., 132:1158, 1984). In order to obtain a cell line suitable for biochemical and genetic studies of CSF-1 signal transduction, clones of BAC1 were established. Clones r...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041300316
更新日期:1987-03-01 00:00:00
abstract::Gastric cancer prognoses are persistently poor due to cancer's penchant to metastasize. As a crucial regulator of signal transducer and activator of transcription (STAT3) signaling, sirtuin 1's (SIRT1) function in gastric cancer has not been well understood. Here, we report upregulated expression of SIRT1 in tissues i...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28186
更新日期:2019-09-01 00:00:00
abstract::Hyperinsulinemia is a likely cause of the increased cancer incidence and mortality in diabetic patients, but its role is difficult to define in vivo. Previous in vitro studies testing the mitogenic potential of insulin and its analogs provided incomplete and sometimes contradictory results. To better evaluate cancer c...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24635
更新日期:2014-11-01 00:00:00
abstract::Dental follicle cells (DFCs) activate and recruit osteoclasts for tooth development and tooth eruption, whereas DFCs themselves differentiate into osteoblasts to form alveolar bone surrounding tooth roots through the interaction with Hertwig's epithelial root sheath (HERS). Also during tooth development, parathyroid h...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27857
更新日期:2019-07-01 00:00:00
abstract::Oxygen tension plays an important role in the regulation of cellular processes. During hematopoietic stem cell (HSC) differentiation, HSCs migrate from one stem cell niche to the next, each with a different oxygen tension that determines which signaling pathways are on and off, determining the differentiation stage of...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24348
更新日期:2013-09-01 00:00:00
abstract::Characteristic features like self-renewal, multilineage differentiation potential, and immune-modulatory/anti-inflammatory properties, besides the ability to mobilize and home distant tissues make stem cells (SCs) a lifeline for an individual. Stem cells (SCs) if could be harvested and expanded without any abnormal ch...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.27846
更新日期:2019-06-01 00:00:00
abstract::We evaluated the role of CCL20 (MIP-3alpha) chemokine in cells directly involved in the remodeling of bone tissue (osteoblasts and osteoclasts) and we confirmed its expression in the subchondral bone tissue of rheumatoid arthritis (RA) patients. The expression of CCL20 and of its receptor CCR6 was evaluated in osteobl...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20905
更新日期:2007-03-01 00:00:00
abstract::We tested the expression of genes coding receptors of a cutaneous serotoninergic/melatoninergic system in whole human skin and in normal and pathologic cultured skin cells. Evaluation of serotonin (5HT), melatonin (MT), and melatonin-related receptors (MRR) showed expression of the isoforms 5HT2B, 5HT7, and MT1 genes ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10287
更新日期:2003-07-01 00:00:00
abstract::Addition of bombesin in the presence of either forskolin or cholera toxin caused a marked (4-6 fold) enhancement of cAMP accumulation in Swiss 3T3 cells. This effect was time and concentration dependent, induced by various bombesin-like peptides and blocked by a bombesin antagonist. Enhancement of cAMP accumulation by...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041370203
更新日期:1988-11-01 00:00:00
abstract::The clonal hematopoiesis when occurring without hematologic abnormalities is defined as clonal hematopoiesis of indeterminate potential (CHIP). Aging causes accumulation of somatic mutations, and hematopoietic stem cells (HSCs) can develop clonal expansion of different lineages by these mutations. CHIP has a correlati...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.27752
更新日期:2019-06-01 00:00:00
abstract::We reported that Disabled-2 (Dab2) is located at the apical membrane in suckling rat intestine. Here, we discovered that, in colon of suckling and adult mouse and of adult human, Dab2 is only at lateral crypt cell membrane and colocalized with E-cadherin. Dab2 depletion in Caco-2 cells led to E-cadherin internalizatio...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29917
更新日期:2021-02-01 00:00:00
abstract::We have previously shown that extracellular ATP acts as a mitogen via protein kinase C (PKC)-dependent and independent pathways (Wang, D., Huang, N., Gonzalez, F.A., and Heppel, L.A. Multiple signal transduction pathways lead to extracellular ATP-stimulated mitogenesis in mammalian cells. I. Involvement of protein kin...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041460320
更新日期:1991-03-01 00:00:00