Abstract:
:Alpha-2-glycoprotein 1, zinc-binding (AZGP1), known as zinc-alpha-2-glycoprotein (ZAG), is a multifunctional secretory glycoprotein and relevant to cancer metastasis. Little is known regarding the underlying mechanisms of AZGP1 in prostate cancer (PCa). In the present study, we report that AZGP1 is an androgen-responsive gene, which is involved in AR-induced PCa cell proliferation and metastasis. In clinical specimens, the expression of AZGP1 in PCa tissues is markedly higher than that in adjacent normal tissues. In cultures, expression of AZGP1 is upregulated by the androgen-AR axis at both messenger RNA and protein levels. Furthermore, Chip-Seq assay identifies canonical androgen-responsive elements (AREs) at AZGP1 enhancer; and dual-luciferase reporter assays reveal that the AREs is highly responsive to androgen whereas mutations of the AREs abolish the reporter activity. In addition, AZGP1 promotes G1/S phase transition and cell cycle progress by increasing cyclin D1 levels in PCa cells. Functional studies demonstrate that knocking down endogenous AZGP1 expression in LNCaP and CWR22Rv1 cells largely weaken androgen/AR axis-induced cell migration and invasion. In vivo xenotransplantation tumor experiments also show that AZGP1 involves in androgen/AR axis-mediated PCa cell proliferation. Taken together, our study implicates for the first time that AZGP1 is an AR target gene and is involved in androgen/AR axis-mediated cell proliferation and metastasis in primary PCa.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Cao R,Ke M,Wu Q,Tian Q,Liu L,Dai Z,Lu S,Liu Pdoi
10.1002/jcp.28366subject
Has Abstractpub_date
2019-08-01 00:00:00pages
17444-17458issue
10eissn
0021-9541issn
1097-4652journal_volume
234pub_type
杂志文章abstract::Liver fibrosis is a reversible wound-healing response that occurs after liver injury. NADPH oxidases (NOXs) and reactive oxygen species (ROS) which are expressed in hepatocytes (HCs), hepatic stellate cells (HSCs), and Kupffer cells (KCs) play an important role in the development of hepatic fibrosis. In in vitro studi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26541
更新日期:2018-10-01 00:00:00
abstract::The aim of the yearly National Congress of the Italian Society of Virology (SIV) is to promote the discussion between senior and younger researchers to improve the knowledge and scientific collaboration among the various areas of Virology. The invited and selected lecturers of the fourth National Congress of SIV cover...
journal_title:Journal of cellular physiology
pub_type:
doi:10.1002/jcp.20430
更新日期:2005-09-01 00:00:00
abstract::Human dental pulp stem cells (hDPSCs) show significant potential for exploitation in novel regeneration strategies, although lack of understanding of their responses to bacterial challenge constrains their application. The present study aimed to investigate whether lipopolysaccharide (LPS), the major pathogenic factor...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24732
更新日期:2015-03-01 00:00:00
abstract::The transient nuclear localization of metallothionein during cell growth and differentiation may be related to the increased requirement of zinc for DNA synthesis, activation of metalloenzymes, and transcription factors. Treatment of 3T3-L1 fibroblasts with aphidicolin, an inhibitor of nuclear DNA synthesis, caused a ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(200005)183:2<247::AID-JCP1
更新日期:2000-05-01 00:00:00
abstract::The polyphenolic compounds curcumin and quercetin increased sensitivity of ovarian cancer cells (CAOV3 and SKOV3) to cisplatin. The effect was obtained when the compounds were added simultaneously with cisplatin, as well as when they were added 24 h before. High serum levels of certain cytokines, for example interleuk...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10186
更新日期:2003-01-01 00:00:00
abstract::Besides the liver, it has been difficult to identify which organ(s) and/or cellular component(s) contribute significantly to the production of human FVIII:c (FVIII). Thus far, only endothelial cells have been shown to constitute a robust extrahepatic source of FVIII, possibly explaining both the diverse presence of FV...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24247
更新日期:2013-05-01 00:00:00
abstract::In novel coronavirus disease 2019 (COVID-19), the increased frequency and overactivation of T helper (Th) 17 cells and subsequent production of large amounts of proinflammatory cytokines result in hyperinflammation and disease progression. The current study aimed to investigate the therapeutic effects of nanocurcumin ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30233
更新日期:2020-12-28 00:00:00
abstract::Epithelial-mesenchymal transition (EMT), via activation of Wnt signaling, is prevailing in embryogenesis, but postnatally it only occurs in pathological processes, such as in tissue fibrosis and tumor metastasis. Our prior studies led us to speculate that EMT might be involved in the loss of limbal epithelial stem cel...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24126
更新日期:2013-01-01 00:00:00
abstract::Eukaryotic translation initiation factor 5A (eIF5A) has a unique character: the presence of an unusual amino acid, hypusine, which is formed by post-translational modifications. Even before the identification of hypusination in eIF5A, the correlation between hypusine formation and protein synthesis, shifting cell prol...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22229
更新日期:2010-11-01 00:00:00
abstract::Previous studies indicate that glutamine is a critical requirement for cell proliferation in vitro. We recently showed that depletion of glutamine from the culture medium supporting growing cells significantly reduced the proportion of cells undergoing DNA synthesis. Similarly glutamine depletion significantly reduced...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041200218
更新日期:1984-08-01 00:00:00
abstract::Our main objective is probing the effect of methylation of CLEC14A on its expression and lung adenocarcinoma (LUAD) progression. Microarray analysis was utilized to screen out differentially downregulated genes with hypermethylation in LUAD tissues. The CLEC14A expression level was measured by western blot analysis an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27112
更新日期:2019-03-01 00:00:00
abstract::Adhesion of calf lens epithelial cells to lens capsule, their natural basement membrane was found to be considerably more rapid than either to plastic or to type I or type IV collagen coated surfaces. No polarity of the basement membrane was observed as the cells were able to attach to either side of the anterior or p...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041130215
更新日期:1982-11-01 00:00:00
abstract::Resistin, firstly reported as an adipocyte-specific hormone, is suggested to be an important link between obesity and diabetes. Recent studies have suggested an association between resistin and atherogenic processes. The adhesion of circulating monocytes to endothelial cells is a critical step in the early stages of a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22555
更新日期:2011-08-01 00:00:00
abstract::NAD(P) is an indispensable cofactor for all organisms and its biosynthetic pathways are proposed as promising novel antibiotics targets against pathogens such as Mycobacterium tuberculosis. Six NAD(P) biosynthetic pathways were reconstructed by comparative genomics: de novo pathway (Asp), de novo pathway (Try), NmR pa...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.22419
更新日期:2011-02-01 00:00:00
abstract::A variant of the hypoxanthine-guanine phosphoribosyltransferase deficient, and adenine phosphoribosyltransferase deficient mouse resistant to 6-azauridine. These cells are not only resistant to 6-azauridine (5 X 10(-4) M), but also to adenosine (10(-3) M). Resistance persists indefinitely even in the absence of both c...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040860202
更新日期:1975-10-01 00:00:00
abstract::Experiments were carried out to test the hypothesis that cell spreading and phagocytosis are similar cell responses to different-sized substrata. The following morphological and biochemical studies provided evidence for this supposition. Cells phagocytosed 1.09-micron and 5.7-micron latex beads, but were unable to com...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041190110
更新日期:1984-04-01 00:00:00
abstract::The unique vascular supply of the retina, the ability to visualize the vasculature in vivo, and the ability to selectively express genes in the retina make the retina an ideal model system to study molecular mechanisms of angiogenesis. In addition, this area of investigation has great clinical significance, because re...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/1097-4652(200009)184:3<301::AID-JCP3>3.0.C
更新日期:2000-09-01 00:00:00
abstract::The development of spike potential mechanisms during cell differentiation was studied in chick myotubes formed in vitro from trypsin-dissociated myoblasts. The spike potential and its rate of rise were measured in myotubes from 4-14 day old cultures. A depolarizing current pulse was delivered to evoke the spike potent...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040900307
更新日期:1977-03-01 00:00:00
abstract::The carboxyl-terminal domain (CTD) of the largest subunit of mammalian RNA polymerase II (RNAP II) consists of 52 repeats of a consensus heptapeptide and is subject to phosphorylation and dephosphorylation events during each round of transcription. RNAP II activity is regulated during the cell cycle and cell cycle-dep...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.10058
更新日期:2002-02-01 00:00:00
abstract::Glioblastoma, the most common brain tumor, is characterized by high proliferation rate, invasion, angiogenesis, and chemo- and radio-resistance. One of most remarkable feature of glioblastoma is the switch toward a glycolytic energetic metabolism that leads to high glucose uptake and consumption and a strong productio...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25421
更新日期:2017-02-01 00:00:00
abstract::We have investigated the kinetics of fluid-phase endocytosis and diacytosis in confluent monolayers of human fibroblasts by comparing the behavior of three markers that have been previously used to study this process: [14C]sucrose, 125I-labeled polyvinylpyrrolidone ([125I]PVP), and Lucifer Yellow. Three distinct kinet...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041360302
更新日期:1988-09-01 00:00:00
abstract::The presence of an appropriate number of viable cells is prerequisite for successive differentiation during chondrogenesis. Chondrogenic differentiation has been reported to be influenced by mechanical stimuli. This research aimed to study the effects of cyclic compressive stress on cell viability of rat bone marrow-d...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24359
更新日期:2013-09-01 00:00:00
abstract::Human immunodeficiency virus (HIV) is one of the critical infectious agents with thousands of newly infected people worldwide. High mutational capability and rapid diversification, inhibition of humoral and cellular immune responses, and thus inability for recognition of an immunogenic region in the viral envelope by ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28280
更新日期:2019-02-18 00:00:00
abstract::The insulin-like growth factors (IGFs) are potent mitogens for malignant cell proliferation. The majority of secreted IGFs are bound to specific IGF-binding proteins (IGFBPs) that are secreted by a large number of cells. These proteins may either inhibit or enhance IGF actions. Breast carcinoma cells secrete a variety...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041580110
更新日期:1994-01-01 00:00:00
abstract::A linear pathway of morphologically intermediate cells has been identified between the cuboidal epithelial stem cells and the doming alveolar-like cultures of the cell line Rat Mammary (Rama) 25 in the order: cuboidal----grey----dark----dark droplet cell----doming cultures. The overall process can be accelerated by di...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041240324
更新日期:1985-09-01 00:00:00
abstract::We examined the importance of tyrosine kinase(s) on the ATP-evoked Ca2+ entry and DNA synthesis of thyroid FRTL-5 cells. ATP rapidly and transiently tyrosine phosphorylated a 72-kDa protein(s). This phosphorylation was abolished by pertussis toxin and by the tyrosine kinase inhibitor genistein, and was dependent on Ca...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199805)175:2<211::AID-JCP1
更新日期:1998-05-01 00:00:00
abstract::Sirtuin 3 (SIRT3) a mitochondrial enzyme that plays an important role in energy homeostasis, cardiac remodeling, and heart failure (HF). The expression of SIRT3 declines with advanced age, cardiovascular, and metabolic diseases. Accumulating evidence suggests that SIRT3 plays a critical role in protecting the heart fr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.27200
更新日期:2019-03-01 00:00:00
abstract::Stimulation of postconfluent Swiss 3T3 cells in serum-free medium with 4.3 mM Ca2+ results in marked increases in both released and cell-associated plasminogen activator (PA). Increased release of PA commenced approximately 10 to 12 hours post-stimulation and continued to increase steadily until 48 hours at which time...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041000309
更新日期:1979-09-01 00:00:00
abstract::Long noncoding RNAs have been reported to be important regulators in numerous cancers. In this study, we found that HOXC13 antisense RNA (HOXC13-AS) was highly expressed in head and neck squamous carcinoma (HNSC) tissues in The Cancer Genome Atlas database. Nasopharyngeal carcinoma (NPC) belongs to HNSC. Therefore, we...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27915
更新日期:2019-08-01 00:00:00
abstract::Although immortalized cultured cells are useful for various functional assays or transcriptome analysis, highly efficient and reproducible immortalization methods have not been developed in avian-derived cells. We introduced the simian virus 40 T antigen (SV40T) and human papillomavirus (HPV)-E6E7 to chick and Okinawa...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27417
更新日期:2019-05-01 00:00:00