Expression profiles of micro RNA in proliferating and differentiating 32D murine myeloid cells.

Abstract:

:32D cells are murine myeloid cells that grow indefinitely in Interleukin-3 (IL-3). In these cells, the type 1 insulin-like growth factor (IGF-I) and granulocytic-colony stimulating factor (G-CSF) induce differentiation to granulocytes. 32D cells do not express insulin receptor substrate-1 (IRS-1) or IRS-2, docking proteins of the IGF-I receptor. Ectopic expression of IRS-1 in these cells inhibits differentiation, the cells become IL-3 independent and IGF-1 dependent and can form tumors in mice. 32D and 32D-derived cells offer a good model in which to study the expression profiles of Micro Rna (miR) related to sustained proliferation or differentiation. We present here the data obtained with miR micro-arrays and identify the miR that are regulated by IGF-1 or G-CSF and are associated with either differentiation or indefinite cell proliferation of 32D murine myeloid cells.

journal_name

J Cell Physiol

authors

Shi B,Prisco M,Calin G,Liu CG,Russo G,Giordano A,Baserga R

doi

10.1002/jcp.20613

keywords:

subject

Has Abstract

pub_date

2006-06-01 00:00:00

pages

706-10

issue

3

eissn

0021-9541

issn

1097-4652

journal_volume

207

pub_type

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