Abstract:
:32D cells are murine myeloid cells that grow indefinitely in Interleukin-3 (IL-3). In these cells, the type 1 insulin-like growth factor (IGF-I) and granulocytic-colony stimulating factor (G-CSF) induce differentiation to granulocytes. 32D cells do not express insulin receptor substrate-1 (IRS-1) or IRS-2, docking proteins of the IGF-I receptor. Ectopic expression of IRS-1 in these cells inhibits differentiation, the cells become IL-3 independent and IGF-1 dependent and can form tumors in mice. 32D and 32D-derived cells offer a good model in which to study the expression profiles of Micro Rna (miR) related to sustained proliferation or differentiation. We present here the data obtained with miR micro-arrays and identify the miR that are regulated by IGF-1 or G-CSF and are associated with either differentiation or indefinite cell proliferation of 32D murine myeloid cells.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Shi B,Prisco M,Calin G,Liu CG,Russo G,Giordano A,Baserga Rdoi
10.1002/jcp.20613keywords:
subject
Has Abstractpub_date
2006-06-01 00:00:00pages
706-10issue
3eissn
0021-9541issn
1097-4652journal_volume
207pub_type
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