A new apoptotic pathway for the complement factor B-derived fragment Bb.


:Apoptosis is involved in both the cellular and humoral immune system destroying tumors. An apoptosis-inducing factor from HL-60 myeloid leukemia cells was obtained, purified, and sequenced. The protein found has been identified as a human complement factor B-derived fragment Bb, although it is known that factor B is able to induce apoptosis in several leukemia cell lines. Monoclonal antibodies against fragment Ba and Bb inhibited the apoptotic activity of factor B. When the purified fragment Bb was used for apoptosis induction, only the anti-Bb antibody inhibited Bb-induced apoptosis, and not the anti-Ba antibody. The apoptosis-inducing activity was found to be enhanced under conditions facilitating the formation of Bb. Blocking TNF/TNFR or FasL/Fas interactions did not interfere with the factor B-induced apoptosis. CD11c (iC3bR) acts as the main subunit of a heterodimer binding to fragment Bb in the apoptosis pathway, and the factor B-derived fragment Bb was found to possess the previously unknown function of inducing apoptosis in leukemic cells through a suicide mechanism of myeloid lineage cells during the differentiation stage.


J Cell Physiol


Uwai M,Terui Y,Mishima Y,Tomizuka H,Ikeda M,Itoh T,Mori M,Ueda M,Inoue R,Yamada M,Hayasawa H,Horiuchi T,Niho Y,Matsumoto M,Ishizaka Y,Ikeda K,Ozawa K,Hatake K





Has Abstract


2000-11-01 00:00:00














  • Lens epithelial cell adhesion to lens capsule: a model system for cell-basement membrane interaction.

    abstract::Adhesion of calf lens epithelial cells to lens capsule, their natural basement membrane was found to be considerably more rapid than either to plastic or to type I or type IV collagen coated surfaces. No polarity of the basement membrane was observed as the cells were able to attach to either side of the anterior or p...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Cammarata PR,Spiro RG

    更新日期:1982-11-01 00:00:00

  • Caveolin enhances hepatocellular carcinoma cell metabolism, migration, and invasion in vitro via a hexokinase 2-dependent mechanism.

    abstract::The development and progression of hepatocellular carcinoma (HCC) have been associated with abnormal cellular metabolism. Gene Expression Profiling Interactive Analysis RNA sequencing data revealed caveolin-1 (CAV-1) and hexokinase 2 (HK2) messenger RNA (mRNA) were significantly upregulated in human HCC compared with ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chai F,Li Y,Liu K,Li Q,Sun H

    更新日期:2019-02-01 00:00:00

  • IGF-1 enhances a store-operated Ca2+ channel in skeletal muscle myoblasts: involvement of a CD20-like protein.

    abstract::Overexpression of IGF-1 in C2C12 myoblasts causes hypertrophy when myoblasts fuse to form myotubes, a response that requires elevated intracellular calcium. We show that myoblasts contain a store-operated Ca2+ channel (SOCC) whose activity is enhanced with IGF-1 overexpression. A membrane protein, CD20, can cause Ca2+...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ju YK,Wu MJ,Chaulet H,Marciniec T,Graham RM,Allen DG

    更新日期:2003-10-01 00:00:00

  • Hypoxia impairs cell fusion and differentiation process in human cytotrophoblast, in vitro.

    abstract::During human pregnancy, the trophoblast develops from differentiation of cytotrophoblast cells into an endocrine active syncytiotrophoblast. In culture, isolated mononuclear cytotrophoblasts aggregate and then fuse to form a syncytium, reproducing the in vivo process. In this study, we examined the effect of low oxyge...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Alsat E,Wyplosz P,Malassiné A,Guibourdenche J,Porquet D,Nessmann C,Evain-Brion D

    更新日期:1996-08-01 00:00:00

  • Effect of strain on human keratinocytes in vitro.

    abstract::Tissue expansion, a technique to enlarge the skin surface area with an expandable balloon, has been widely used in reconstructive surgery. Although the effect of tissue expansion on in vivo skin physiology and histology has been well documented, it remains unclear whether keratinocytes or other cell types are responsi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Takei T,Rivas-Gotz C,Delling CA,Koo JT,Mills I,McCarthy TL,Centrella M,Sumpio BE

    更新日期:1997-10-01 00:00:00

  • Non-Metabolic Role of PKM2 in Regulation of the HIV-1 LTR.

    abstract::Identification of cellular proteins, in addition to already known transcription factors such as NF-κB, Sp1, C-EBPβ, NFAT, ATF/CREB, and LEF-1, which interact with the HIV-1 LTR, is critical in understanding the mechanism of HIV-1 replication in monocytes/macrophages. Our studies demonstrate upregulation of pyruvate ki...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sen S,Deshmane SL,Kaminski R,Amini S,Datta PK

    更新日期:2017-03-01 00:00:00

  • Extracellular acidification enhances DNA binding activity of MafG-FosB heterodimer.

    abstract::Cells are quite sensitive to a change of the extracellular pH and respond to it through detection of the H+/HCO3- level in extracellular fluid. However, little is known about molecular details induced by acidosis, such as intracellular pathways and gene expression. Here we describe properties of gene expression, prote...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Shimokawa N,Kumaki I,Qiu CH,Ohmiya Y,Takayama K,Koibuchi N

    更新日期:2005-10-01 00:00:00

  • Antibodies Against Mimotopes of Simian Virus 40 Large T Antigen, the Oncoprotein, in Serum Samples From Elderly Healthy Subjects.

    abstract::Simian Virus 40 (SV40), a monkey polyomavirus, was administered to human populations by early anti-poliomylitis vaccines contaminated by this small DNA tumor virus. Data on SV40 infection in humans remain controversial. Elderly subjects represent an interesting cohort to investigate, because they were not immunized wi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mazzoni E,Guerra G,Casali MV,Pietrobon S,Bononi I,Puozzo A,Tagliapietra A,Nocini PF,Tognon M,Martini F

    更新日期:2017-01-01 00:00:00

  • High salt-induced excess reactive oxygen species production resulted in heart tube malformation during gastrulation.

    abstract::An association has been proved between high salt consumption and cardiovascular mortality. In vertebrates, the heart is the first functional organ to be formed. However, it is not clear whether high-salt exposure has an adverse impact on cardiogenesis. Here we report high-salt exposure inhibited basement membrane brea...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Gao LR,Wang G,Zhang J,Li S,Chuai M,Bao Y,Hocher B,Yang X

    更新日期:2018-09-01 00:00:00

  • Evidence that density-dependent growth arrest is a two-stage process in WI-38 cells.

    abstract::It was the goal of this study to determine whether during long-term quiescence WI-38 cells gradually lose labile components which then need to be resynthesized before a stimulated cell can progress through G-1 and enter S. The metabolic and molecular status of WI-38 cells was systematically analyzed as they entered an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Owen TA,Carter R,Whitman MM,Soprano DR,Soprano KJ

    更新日期:1990-01-01 00:00:00

  • Human bronchial epithelial cells synthesize cholesterol sulfate during squamous differentiation in vitro.

    abstract::Epithelial cells of the airways can, under pathological conditions, undergo squamous metaplasia. The accumulation of cholesterol sulfate has recently been described as a new marker for squamous cell differentiation in rabbit tracheal epithelial cells. We now report that normal human bronchial epithelial cells in cultu...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Rearick JI,Hesterberg TW,Jetten AM

    更新日期:1987-12-01 00:00:00

  • Biphasic effects of 17-beta-estradiol on expression of occludin and transendothelial resistance and paracellular permeability in human vascular endothelial cells.

    abstract::Tight junctions govern the paracellular permeability of endothelial and epithelial cells. Aberrations of tight junction function are an early and key event during the vascular spread of cancer and inflammation. This study sought to determine the role of estrogen in the regulation of tight junctions and expression of m...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ye L,Martin TA,Parr C,Harrison GM,Mansel RE,Jiang WG

    更新日期:2003-08-01 00:00:00

  • THP-1 macrophage membrane-bound plasmin activity is up-regulated by transforming growth factor-beta 1 via increased expression of urokinase and the urokinase receptor.

    abstract::Receptors for urokinase (uPA) and plasminogen provide a mechanism to direct the cellular activation of plasminogen. The regulation of these receptors is important for several macrophage functions. In these studies, the effect of transforming growth factor-beta 1 (TGF-beta 1) on uPA, uPA receptor, and plasminogen recep...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Falcone DJ,McCaffrey TA,Mathew J,McAdam K,Borth W

    更新日期:1995-08-01 00:00:00

  • Growth-stimulating effect of catecholamines on rat aortic smooth muscle cells in culture.

    abstract::The effect of epinephrine was tested on the proliferation of rat arterial smooth muscle cells (SMC) in secondary cultures. Epinephrine added daily to the culture medium caused a striking stimulation of growth. The effect increased with time and was dose-dependent. Maximal stimulation was observed at a concentration of...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Blaes N,Boissel JP

    更新日期:1983-08-01 00:00:00

  • Immunomodulatory effects of nanocurcumin on Th17 cell responses in mild and severe COVID-19 patients.

    abstract::In novel coronavirus disease 2019 (COVID-19), the increased frequency and overactivation of T helper (Th) 17 cells and subsequent production of large amounts of proinflammatory cytokines result in hyperinflammation and disease progression. The current study aimed to investigate the therapeutic effects of nanocurcumin ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tahmasebi S,El-Esawi MA,Mahmoud ZH,Timoshin A,Valizadeh H,Roshangar L,Varshoch M,Vaez A,Aslani S,Navashenaq JG,Aghebati-Maleki L,Ahmadi M

    更新日期:2020-12-28 00:00:00

  • Interaction of high-molecular-weight basic fibroblast growth factor with endothelium: biological activity and intracellular fate of human recombinant M(r) 24,000 bFGF.

    abstract::The single-copy gene of human basic fibroblast growth factor (bFGF) encodes four co-expressed isoforms, with an apparent molecular weight (M(r)) of 24 kD, 22.5 kD, 22 kD, and 18 kD, co-translated from a single mRNA. As a tool for the study of the role exerted by the different bFGF isoforms in the biology of endothelia...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Gualandris A,Urbinati C,Rusnati M,Ziche M,Presta M

    更新日期:1994-10-01 00:00:00

  • Homocysteine-induced myofibroblast differentiation in mouse aortic endothelial cells.

    abstract::Differentiation of myofibroblast, as evidenced by alpha-smooth muscle actin (alpha-SMA) expression, is largely mediated by transforming growth factor-beta1 (TGF-beta1). This mechanism often follows inflammatory events such as endothelial damage due to oxidative stress, which can further leads to vascular thickening, s...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sen U,Moshal KS,Tyagi N,Kartha GK,Tyagi SC

    更新日期:2006-12-01 00:00:00

  • The role of miR-34 in cancer drug resistance.

    abstract::Resistance to conventional chemotherapy remains a major cause of cancer relapse and cancer-related deaths. Therefore, there is an urgent need to overcome resistance barriers. To improve cancer treatment approaches, it is critical to elucidate the basic mechanisms underlying drug resistance. Increasingly, the mechanism...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Naghizadeh S,Mohammadi A,Duijf PHG,Baradaran B,Safarzadeh E,Cho WC,Mansoori B

    更新日期:2020-10-01 00:00:00

  • Differential role of Rho GTPases in intestinal epithelial barrier regulation in vitro.

    abstract::Maintenance of intestinal epithelial barrier functions is crucial to prevent systemic contamination by microbes that penetrate from the gut lumen. GTPases of the Rho-family such as RhoA, Rac1, and Cdc42 are known to be critically involved in the regulation of intestinal epithelial barrier functions. However, it is sti...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Schlegel N,Meir M,Spindler V,Germer CT,Waschke J

    更新日期:2011-05-01 00:00:00

  • Glucosamine promotes osteogenic differentiation of dental pulp stem cells through modulating the level of the transforming growth factor-beta type I receptor.

    abstract::Dental pulp stem cells (DPSCs) are clonogenic, self-renewing, and multi-potential DPSCs capable of differentiating into osteoblasts. In this study, primary cell cultures were obtained from human dental pulp tissue of developing third molars, and flow cytometry was used to sort the subpopulation of DPSCs with STRO-1 an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Huang CH,Tseng WY,Yao CC,Jeng JH,Young TH,Chen YJ

    更新日期:2010-10-01 00:00:00

  • Corneal endothelium tissue engineering: An evolution of signaling molecules, cells, and scaffolds toward 3D bioprinting and cell sheets.

    abstract::Cornea is an avascular and transparent tissue that focuses light on retina. Cornea is supported by the corneal-endothelial layer through regulation of hydration homeostasis. Restoring vision in patients afflicted with corneal endothelium dysfunction-mediated blindness most often requires corneal transplantation (CT), ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Khalili M,Asadi M,Kahroba H,Soleyman MR,Andre H,Alizadeh E

    更新日期:2020-10-08 00:00:00

  • Elk-1 associates with FAK, regulates the expression of FAK and MAP kinases as well as apoptosis in HK-2 cells.

    abstract::Focal adhesion kinase (FAK), MAP kinases and the nuclear transcription factor Elk-1 have been reported to be implicated in the same cellular processes, however, their direct or indirect interaction and potential function(s) has not been documented. Here, we explored the association of FAK with Elk-1, the implication o...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mamali I,Kotsantis P,Lampropoulou M,Marmaras VJ

    更新日期:2008-07-01 00:00:00

  • Prostaglandin F2α: a bone remodeling mediator.

    abstract::Prostaglandin F2α (PGF2α) plays multiple roles on bone metabolism by regulating a wide range of signaling pathways. PGF2α, via activation of PKC, stimulates Na-dependent inorganic phosphate (Pi) transport system in osteoblasts; up-regulates interleukin (IL)-6 synthesis; increases vascular endothelial growth factor (VE...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Agas D,Marchetti L,Hurley MM,Sabbieti MG

    更新日期:2013-01-01 00:00:00

  • Assembly of the peripheral domain of the bovine vacuolar H(+)-adenosine triphosphatase.

    abstract::The biosynthesis and assembly of the peripheral sector (V1) of the vacuolar proton-translocating adenosine triphosphatase (V-ATPase) was studied in a bovine kidney epithelial cell line. Monolayer cultures of cells were metabolically radiolabeled with Tran35S-label and the V-ATPase subsequently immunoprecipitated using...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Myers M,Forgac M

    更新日期:1993-07-01 00:00:00

  • Loss of shear stress induces leukocyte-mediated cytokine release and blood-brain barrier failure in dynamic in vitro blood-brain barrier model.

    abstract::Brain ischemia is associated with an acute release of pro-inflammatory cytokines, notably TNF-alpha and IL-6 and failure of the blood-brain barrier. Shear stress, hypoxia-hypoglycemia, and blood leukocytes play a significant role in blood-brain barrier failure during transient or permanent ischemia. However, these mec...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Krizanac-Bengez L,Mayberg MR,Cunningham E,Hossain M,Ponnampalam S,Parkinson FE,Janigro D

    更新日期:2006-01-01 00:00:00

  • Loss of growth responsiveness to epidermal growth factor and enhanced production of alpha-transforming growth factors in ras-transformed mouse mammary epithelial cells.

    abstract::A mouse mammary epithelial cell line, NMuMG, exhibits a low capacity to grow in semisolid medium as colonies and it is not tumorigenic in nude mice. In contrast, NMuMG cells which have been transformed by an activated c-Harvey ras proto-oncogene, NMuMG/rasH, or by the polyoma middle T-transforming gene, NMuMG/pyt, are...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Salomon DS,Perroteau I,Kidwell WR,Tam J,Derynck R

    更新日期:1987-03-01 00:00:00

  • Emerging functions of p21-activated kinases in human cancer cells.

    abstract::The p21 activated kinases (Paks), an evolutionarily conserved family of serine/threonine kinases, are important for a variety of cellular functions including cell morphogenesis, motility, survival, mitosis, and angiogenesis. Paks are widely expressed in numerous tissues and are activated by growth factors and extracel...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Kumar R,Vadlamudi RK

    更新日期:2002-11-01 00:00:00

  • Galectin-3 deficiency protects pancreatic islet cells from cytokine-triggered apoptosis in vitro.

    abstract::Beta cell apoptosis is a hallmark of diabetes. Since we have previously shown that galectin-3 deficient (LGALS3(-/-) ) mice are relatively resistant to diabetes induction, the aim of this study was to examine whether beta cell apoptosis depends on the presence of galectin-3 and to delineate the underlying mechanism. D...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Saksida T,Nikolic I,Vujicic M,Nilsson UJ,Leffler H,Lukic ML,Stojanovic I,Stosic-Grujicic S

    更新日期:2013-07-01 00:00:00

  • Insulin and growth factors stimulate rapid posttranslational changes in glucose transport in ovarian granulosa cells.

    abstract::The glucose analogues, 3-O-methyl-D-glucose and 2-deoxy-D-glucose, have been used to characterize glucose transport and its regulation by serum and growth factors in monolayer cultures of granulosa cells obtained from bovine ovaries. Uptake of 3-O-methylglucose was shown to be independent of the Na+-gradient, independ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Allen WR,Nilsen-Hamilton M,Hamilton RT

    更新日期:1981-07-01 00:00:00

  • Generation of tumor-specific cytotoxic T-lymphocytes from the peripheral blood of colorectal cancer patients for adoptive T-cell transfer.

    abstract::This study designs a strategy for an adoptive cellular therapy (ACT) protocol based on the ex-vivo selection of autologous peripheral blood-derived CD8-enriched T-cells, stimulated with dendritic cells (DCs) that had been pulsed with apoptotic tumor cells to generate cytotoxic T lymphocytes (CTLs) with anti-tumor acti...

    journal_title:Journal of cellular physiology

    pub_type: 临床试验,杂志文章


    authors: Carluccio S,Delbue S,Signorini L,Setola E,Bagliani A,Della Valle A,Galli A,Ferrante P,Bregni M

    更新日期:2015-07-01 00:00:00