Direct evidence for nuclear and cytoplasmic colocalization of proteasomes (multiprotease complexes) in liver.


:Subcellular localization of the large multicatalytic protease complexes called proteasomes, which have been found in soluble fractions of various cells, was examined by biochemical, immunological, and immunohistological methods. Rat liver nuclei, purified by two different procedures, showed high activities for degrading [3H]methylcasein and various fluorogenic oligopeptides with neutral and weakly alkaline pH optima. On gel filtration, all of these peptidase activities were recovered in a single peak with the unusually large molecular weight of about 600,000. Properties of the proteolytic activity in crude extracts of the nucleus and the cytoplasm were very similar. Immunoelectrophoretic blot analysis showed the presence of appreciable concentrations of proteasomes with similar immunoreactivity in isolated nuclear and cytosolic fractions. Moreover, immunohistochemical staining of human liver showed that proteasomes were predominantly localized in the nuclear matrix but also were present diffusely in the cytoplasm of hepatocytes. These findings indicate the nuclear and cytoplasmic colocalization of proteasomes.


J Cell Physiol


Tanaka K,Kumatori A,Ii K,Ichihara A




Has Abstract


1989-04-01 00:00:00












  • Cucurbitacin B suppresses proliferation of pancreatic cancer cells by ceRNA: Effect of miR-146b-5p and lncRNA-AFAP1-AS1.

    abstract::Cucurbitacin B (CuB) is a natural tetracyclic triterpene product that displays antitumor activity against a wide variety of cancers. In this study, we explored the antipancreatic cancer activity of CuB via the inhibition of expression of the cancer-related long noncoding RNA, actin filament-associated protein 1-antise...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhou J,Liu M,Chen Y,Xu S,Guo Y,Zhao L

    更新日期:2019-04-01 00:00:00

  • Therapeutics for advanced hepatocellular carcinoma: Recent advances, current dilemma, and future directions.

    abstract::Hepatocellular carcinoma (HCC) is one of the most common malignancies and is a serious threat to people's health worldwide. The prognosis of advanced HCC is dim if left untreated. In the clinic, the treatment options for advanced HCC include surgery, radiotherapy, transcatheter arterial chemoembolization, and so forth...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Zhu J,Yin T,Xu Y,Lu XJ

    更新日期:2019-08-01 00:00:00

  • Coordinate secretion of mouse alphafetoprotein, mouse albumin and rat albumin by mouse hepatoma-rat hepatoma hybrid cells.

    abstract::Mouse heptoma cells that secrete large amounts of alpha-fetoprotein (AFP) and albumin have been crossed with rat hepatoma cells that secret only albumin, and in relatively small amounts, to investigate the influence of each parental genome upon the expression of serum proteins. All of the ten independent hybrid clones...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Cassio D,Hassoux R,Dupiers M,Uriel J,Weiss MC

    更新日期:1980-09-01 00:00:00

  • Zearalenone induces apoptosis and necrosis in porcine granulosa cells via a caspase-3- and caspase-9-dependent mitochondrial signaling pathway.

    abstract::Zearalenone is a mycotoxin produced mainly by Fusarium. There are numerous incidences of mycotoxicosis in laboratory and domestic animals, especially in pigs. However, little is known about molecular mechanisms of zearalenone toxicity. Granulosa cells are the maximal cell population in follicles, and they play an esse...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhu L,Yuan H,Guo C,Lu Y,Deng S,Yang Y,Wei Q,Wen L,He Z

    更新日期:2012-05-01 00:00:00

  • Molecular biology and immunoregulation of human neurotropic JC virus in CNS.

    abstract::The human polyomavirus, JC virus (JCV), provides an excellent model system to investigate the reciprocal interaction of the immune and nervous systems. Infection with JCV occurs during childhood and the virus remains in the latent state with no apparent clinical symptoms. However, under immunosuppressed conditions, th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Sweet TM,Del Valle L,Khalili K

    更新日期:2002-06-01 00:00:00

  • 15-Lipoxygenase promotes chronic hypoxia-induced phenotype changes of PASMCs via positive feedback-loop of BMP4.

    abstract::Our laboratory has previously demonstrated that 15-lipoxygenase (15-LO)/15-hydroxyeicosatetr-aenoic acid (15-HETE) is involved in hypoxic pulmonary arterial hypertension (PAH). Phenotypical alterations of vascular smooth muscle cells are considered to be an important stage in the development of PAH, whereas the underl...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yu X,Wei L,Lu P,Shen T,Liu X,Li T,Zhang B,Yu H,Zhu D

    更新日期:2015-07-01 00:00:00

  • Selective induction of enhanced complement receptor 3 activity on murine macrophages.

    abstract::A high proportion of murine resident peritoneal macrophages bear complement receptors 1 and 3 (CR1, CR3) which bind C3b and iC3b components of complement, respectively. By contrast, macrophages derived from bone marrow, blood, and the elicited peritoneal exudate are predominantly CR1+3. To determine if the microenviro...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yen SE,Walker WS

    更新日期:1983-04-01 00:00:00

  • sp2 -Iminosugar α-glucosidase inhibitor 1-C-octyl-2-oxa-3-oxocastanospermine specifically affected breast cancer cell migration through Stim1, β1-integrin, and FAK signaling pathways.

    abstract::Aberrant glycosylation changes on many glycoproteins are often related to cancer progression and metastasis. sp2 -Iminosugar-type castanospermine analogues, inhibitors of α-glucosidases, have been reported to exhibit antitumor activity. However, their effects on cell migration and the underlying molecular mechanism ar...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Gueder N,Allan G,Telliez MS,Hague F,Fernandez JM,Sanchez-Fernandez EM,Ortiz-Mellet C,Ahidouch A,Ouadid-Ahidouch H

    更新日期:2017-12-01 00:00:00

  • Epidermal growth factor treatment of A431 cells alters the binding capacity and electrophoretic mobility of the cytoskeletally associated epidermal growth factor receptor.

    abstract::The epidermal growth factor (EGF) receptor interacts with structural elements of A431 cells and remains associated with the cytoskeleton following extraction with nonionic detergents. Extraction of cells with 0.15% Triton X-100 resulted in detection of only approximately 40% of the EGF binding sites on the cytoskeleto...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Roy LM,Gittinger CK,Landreth GE

    更新日期:1991-01-01 00:00:00

  • Inhibition of BALB/c-3T3 cells in late G1: commitment to DNA synthesis controlled by somatomedin C.

    abstract::Methylglyoxal bis-(guanylhydrazone) (mGBG) blocked the stimulation of DNA synthesis in quiescent, density-inhibited BALB/c-3T3 cells treated with platelet-derived growth factor (PDGF) and platelet-poor plasma (PPP). Competence formation produced by a transient exposure to PDGF was not effected by mGBG. In contrast, mG...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wharton W,Van Wyk JJ,Pledger WJ

    更新日期:1981-04-01 00:00:00

  • Increased epidermal growth factor receptor in an estrogen-responsive, adriamycin-resistant MCF-7 cell line.

    abstract::We examined the expression of the estrogen and epidermal growth factor (EGF) receptors in a drug-resistant subline of MCF-7 cells in order to study potential alterations in hormone dependence or in the growth factor pathway that could be related to the development of drug resistance in human breast cancer. The drug-re...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dickstein B,Valverius EM,Wosikowski K,Saceda M,Pearson JW,Martin MB,Bates SE

    更新日期:1993-10-01 00:00:00

  • Inhibition of PI 3-kinase and RAS blocks IGF-I and insulin-induced uncoupling protein 1 gene expression in brown adipocytes.

    abstract::Fetal brown adipocytes expressed uncoupling protein 1 (UCP1) mRNA, this expression being blunted throughout culture for 24 h in a serum-free medium. At physiological doses, either insulin-like growth factor I (IGF-I) or insulin turned out to be as potent as dibutyryl cAMP (dbcAMP) in increasing UCP1 gene transcription...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Teruel T,Valverde AM,Navarro P,Benito M,Lorenzo M

    更新日期:1998-07-01 00:00:00

  • Changes in luminal flow rate modulate basal and bradykinin-stimulated cell [Ca2+] in aortic endothelium.

    abstract::Hemodynamic forces influence many endothelial cell functions. The coupling between hemodynamic forces and cell function could be mediated by mechano-sensitive ion channels present in the plasma membrane of endothelial cells. Because one of these channels is permeable to Ca2+, we tested whether hemodynamic forces influ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Oliver JA,Chase HS Jr

    更新日期:1992-04-01 00:00:00

  • Persistent effects of phorbol 12-myristate 13-acetate: possible implication of vesicle traffic.

    abstract::Relative to their normal counterparts, transformed epithelial cells have a distinctive and quantifiable three-dimensional shape. Biophysical and mathematical methods are used to distinguish these extremes in cells from two lines, cultured from rat liver and tracheal epithelium, respectively. Cells adopted a more trans...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Heckman CA,Plummer HK 3rd,Runyeon CS

    更新日期:1996-01-01 00:00:00

  • ATP-resistant variants of transformed mouse fibroblasts.

    abstract::Addition of ATP to cultures of transformed mouse fibroblasts, 3T6 cells, resulted in cell growth inhibition, whereas the growth of the non-transformed counterparts, 3T3 cells, was only slightly affected. The inhibition was found to be specific for adenine nucleotides, and concentration dependent. At relatively low con...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Belzer I,Friedberg I

    更新日期:1989-09-01 00:00:00

  • MFN2 silencing promotes neural differentiation of embryonic stem cells via the Akt signaling pathway.

    abstract::Mitofusin 2 (MFN2) is a regulatory protein participating in mitochondria dynamics, cell proliferation, death, differentiation, and so on. This study aims at revealing the functional role of MFN2 in the pluripotency maintenance and primitive differetiation of embryonic stem cell (ESCs). A dox inducible silencing and ro...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yi S,Cui C,Huang X,Yin X,Li Y,Wen J,Luan Q

    更新日期:2020-02-01 00:00:00

  • Production of transforming growth factor beta by human peripheral blood monocytes and neutrophils.

    abstract::Human peripheral blood monocytes and neutrophils secrete TGF-beta. Activation of monocytes with LPS stimulates the secretion of TGF-beta; however, the production of TGF-beta by neutrophils was not altered by treatment with LPS. The secreted TGF-beta appears to be in a fully active form since acid treatment of the cond...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Grotendorst GR,Smale G,Pencev D

    更新日期:1989-08-01 00:00:00

  • Long noncoding RNA MALAT1 mediates cardiac fibrosis in experimental postinfarct myocardium mice model.

    abstract::Cardiac fibrosis is a pathological remodeling response to myocardial infarction (MI) and impairs cardiac contractility. Long noncoding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is increased in patients with MI. However, the functions of MALAT1 in cardiac fibrosis have not been elucid...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Huang S,Zhang L,Song J,Wang Z,Huang X,Guo Z,Chen F,Zhao X

    更新日期:2019-03-01 00:00:00

  • Perlecan Heparan Sulfate Is Required for the Inhibition of Smooth Muscle Cell Proliferation by All-trans-Retinoic Acid.

    abstract::Smooth muscle cell (SMC) proliferation is a key process in stabilization of atherosclerotic plaques, and during restenosis after interventions. A clearer understanding of SMC growth regulation is therefore needed to design specific anti-proliferative therapies. Retinoic acid has been shown to inhibit proliferation of ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tran-Lundmark K,Tannenberg P,Rauch BH,Ekstrand J,Tran PK,Hedin U,Kinsella MG

    更新日期:2015-02-01 00:00:00

  • Model for the regulation of platelet volume and responsiveness by the trans-membrane Na+/K(+)-pump.

    abstract::The correspondence between K+ uptake in platelets to their responsiveness was studied using 86Rb+ as an analogue of K+. An average 86Rb+ uptake rate of 0.73 (+/- 0.140) x 10(-15) mole Rb+/min-plt (n = 20) was observed. By the use of K(+)-influx inhibitors, we were able to distinguish three distinct 86Rb+ uptake pathwa...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Marx G,Blankenfeld A,Panet R,Gorodetsky R

    更新日期:1992-05-01 00:00:00

  • TGFβ3 regulates periderm removal through ΔNp63 in the developing palate.

    abstract::The periderm is a flat layer of epithelium created during embryonic development. During palatogenesis, the periderm forms a protective layer against premature adhesion of the oral epithelia, including the palate. However, the periderm must be removed in order for the medial edge epithelia (MEE) to properly adhere and ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hu L,Liu J,Li Z,Ozturk F,Gurumurthy C,Romano RA,Sinha S,Nawshad A

    更新日期:2015-06-01 00:00:00

  • LINC00261 suppresses human colon cancer progression via sponging miR-324-3p and inactivating the Wnt/β-catenin pathway.

    abstract::Growing evidence indicates long noncoding RNAs (lncRNAs) are significant regulators in the progression of various malignant tumors including colon cancer. Dysregulation of lncRNA LINC00261 has been identified in many cancers. Investigations on LINC00261 function have revealed that LINC00261 could act as a crucial tumo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yan D,Liu W,Liu Y,Luo M

    更新日期:2019-12-01 00:00:00

  • Functional discontinuities in prothymosin alpha caused by caspase cleavage in apoptotic cells.

    abstract::Our study examines the effect of apoptosis on prothymosin alpha, an abundant, nuclear protein intimately involved with proliferation of all mammalian cells. When HeLa cells were treated with actinomycin D, with etoposide, or with staurosporine following synchronization with hydroxyurea, they underwent apoptosis based ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Enkemann SA,Wang RH,Trumbore MW,Berger SL

    更新日期:2000-02-01 00:00:00

  • Pericyte growth and contractile phenotype: modulation by endothelial-synthesized matrix and comparison with aortic smooth muscle.

    abstract::We compared the effects of endothelial-synthesized matrix and purified matrix molecules on pericyte (PC) and aortic smooth muscle cell (SMC) growth, heparin sensitivity, and contractile phenotype in vitro. When PC are plated on endothelial-synthesized (EC) matrix, cell number is, on average, 3.1-fold higher than ident...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Newcomb PM,Herman IM

    更新日期:1993-05-01 00:00:00

  • Unsaturated fatty acids induce mesenchymal stem cells to increase secretion of angiogenic mediators.

    abstract::Mesenchymal stem cells (MSC) represent emerging cell-based therapies for diabetes and associated complications. Ongoing clinical trials are using exogenous MSC to treat type 1 and 2 diabetes, cardiovascular disease and non-healing wounds due to diabetes. The majority of these trials are aimed at exploiting the ability...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Smith AN,Muffley LA,Bell AN,Numhom S,Hocking AM

    更新日期:2012-09-01 00:00:00

  • Regulation of Indian hedgehog mRNA levels in chondrocytic cells by ERK1/2 and p38 mitogen-activated protein kinases.

    abstract::Indian hedgehog (Ihh) is produced by growth plate pre-hypertrophic chondrocytes, and is an important regulator of endochondral ossification. However, little is known about the regulation of Ihh in chondrocytes. We have examined the role of integrins and mitogen-activated protein (MAP) kinases in Ihh mRNA regulation in...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lai LP,DaSilva KA,Mitchell J

    更新日期:2005-04-01 00:00:00

  • miR-142a-5p promoted osteoblast differentiation via targeting nuclear factor IA.

    abstract::miR-142a-5p plays critical roles in multiple biological processes and diseases, such as inflammation and tumorigenesis. However, it remains to be explored if and how miR-142a-5p contributes to osteoblast differentiation. In this study, our results showed that miR-142a-5p was highly expressed in bone tissue of mice and...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yuan H,Li M,Feng X,Zhu E,Wang B

    更新日期:2021-03-01 00:00:00

  • Proteoglycan biosynthesis is required in BC3H1 myogenic cells for modulation of vascular smooth muscle alpha-actin gene expression in response to microenvironmental signals.

    abstract::Induction of vascular smooth muscle (VSM) alpha-actin mRNA expression during cytodifferentiation of mouse BC3H1 myogenic cells coincides with the accumulation of cell surface- and extracellular matrix-associated sulfated proteoglycans. Inhibition of proteoglycan biosynthesis in myogenic cells using an artificial beta-...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lee SH,Yan H,Reeser JC,Dillman JM,Strauch AR

    更新日期:1995-07-01 00:00:00

  • Gap junctions in human synovial cells and tissue.

    abstract::Our objective was to establish the existence of intercellular communication through gap junctions in synovial lining cells and in primary and passaged cultures of human synovial cells. Communication between cells was assessed using the nystatin perforated-patch method, fluorescent dye transfer, immunochemistry, transm...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kolomytkin OV,Marino AA,Sadasivan KK,Meek WD,Wolf RE,Hall V,McCarthy KJ,Albright JA

    更新日期:2000-07-01 00:00:00

  • Differential proteomic profiling identifies novel molecular targets of pterostilbene against experimental diabetes.

    abstract::Pterostilbene (PTS), a naturally occurring stilbene, confers protection against oxidative and cytokine stress induced pancreatic β-cell apoptosis in vitro and in vivo. To provide insights into the molecular mechanism, we performed a proteomic study on the pancreas of PTS-treated diabetic mice using electrospray ioniza...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dornadula S,Thiruppathi S,Palanisamy R,Umapathy D,Suzuki T,K Mohanram R

    更新日期:2019-03-01 00:00:00