Direct evidence for nuclear and cytoplasmic colocalization of proteasomes (multiprotease complexes) in liver.


:Subcellular localization of the large multicatalytic protease complexes called proteasomes, which have been found in soluble fractions of various cells, was examined by biochemical, immunological, and immunohistological methods. Rat liver nuclei, purified by two different procedures, showed high activities for degrading [3H]methylcasein and various fluorogenic oligopeptides with neutral and weakly alkaline pH optima. On gel filtration, all of these peptidase activities were recovered in a single peak with the unusually large molecular weight of about 600,000. Properties of the proteolytic activity in crude extracts of the nucleus and the cytoplasm were very similar. Immunoelectrophoretic blot analysis showed the presence of appreciable concentrations of proteasomes with similar immunoreactivity in isolated nuclear and cytosolic fractions. Moreover, immunohistochemical staining of human liver showed that proteasomes were predominantly localized in the nuclear matrix but also were present diffusely in the cytoplasm of hepatocytes. These findings indicate the nuclear and cytoplasmic colocalization of proteasomes.


J Cell Physiol


Tanaka K,Kumatori A,Ii K,Ichihara A




Has Abstract


1989-04-01 00:00:00












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    abstract::The caspase recruitment domain (CARD)-containing proteins CARMA1-3 share high degree of sequence, structure and functional homology. Whereas CARMA1 and CARMA3 have been identified as crucial components of signal transduction pathways that lead to activation of NF-κB transcription factor, little is known about the func...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Scudiero I,Zotti T,Ferravante A,Vessichelli M,Vito P,Stilo R

    更新日期:2011-12-01 00:00:00

  • Modulating calcium-mediated NFATc1 and mitogen-activated protein kinase deactivation underlies the inhibitory effects of kavain on osteoclastogenesis and bone resorption.

    abstract::Osteoclasts are responsible for bone resorption during the process of bone remodeling. Increased osteoclast numbers and bone resorption activity are the main factors contributing to bone loss-related diseases such as osteoporosis. Therefore, modulating the formation and function of osteoclasts is critical for the effe...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Guo Q,Cao Z,Wu B,Chen F,Tickner J,Wang Z,Qiu H,Wang C,Chen K,Tan R,Gao Q,Xu J

    更新日期:2018-01-01 00:00:00

  • Conditional knockout of the PDK-1 gene in osteoblasts affects osteoblast differentiation and bone formation.

    abstract::Osteoblasts are the main functional cells of bone formation, and they are responsible for the synthesis, secretion, and mineralization of the bone matrix. Phosphatidylinositol-3-kinase/Akt is an important signaling pathway involved in the regulation of cell proliferation, death, and survival. Some studies have shown t...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bai Y,Zhang Q,Chen Q,Zhou Q,Zhang Y,Shi Z,Nong H,Liu M,Zeng G,Zong S

    更新日期:2020-12-30 00:00:00

  • Syndecans in chronic inflammatory and autoimmune diseases: Pathological insights and therapeutic opportunities.

    abstract::Syndecans (SDCs) are a family of heparan sulfate proteoglycans (HSPGs) glycoproteins ubiquitously expressed on the cell surfaces and extracellular matrix of all mammalian tissues. There are four mammalian syndecans, SDC-1 thorough 4, which play a critical role in cell adhesion, migration, proliferation, differentiatio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Agere SA,Kim EY,Akhtar N,Ahmed S

    更新日期:2018-09-01 00:00:00

  • Abortive transformation of temperature-sensitive mutants of rat 3Y1 cells by simian virus 40: effect of cellular arrest states on entry into S phase and cellular proliferation.

    abstract::Four temperature-sensitive (ts) mutants of rat 3Y1 fibroblasts, representing independent complementation groups, cease to proliferate predominantly with a 2n DNA content, at the restrictive temperature (39.8 degrees C) (temperature arrest) or at the permissive temperature (33.8 degrees C) at a confluent cell density (...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mitsudomi T,Kimura G

    更新日期:1985-06-01 00:00:00

  • Novel biphasic traffic of endocytosed EGF to recycling and degradative compartments in lacrimal gland acinar cells.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Xie J,Qian L,Wang Y,Rose CM,Yang T,Nakamura T,Hamm-Alvarez SF,Mircheff AK

    更新日期:2004-04-01 00:00:00

  • Bombesin enhancement of cAMP accumulation in Swiss 3T3 cells: evidence of a dual mechanism of action.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Millar JB,Rozengurt E

    更新日期:1988-11-01 00:00:00

  • Shepherding AKT and androgen receptor by Ack1 tyrosine kinase.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Mahajan K,Mahajan NP

    更新日期:2010-08-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Gao W,Qi CQ,Feng MG,Yang P,Liu L,Sun SH

    更新日期:2020-10-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Asou H

    更新日期:1992-11-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Jin W,Zhang Y,Liu Z,Che Z,Gao M,Peng H

    更新日期:2021-01-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Babich V,Vadnagara K,Di Sole F

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  • Desferoxamine protects against glucocorticoid-induced osteonecrosis of the femoral head via activating HIF-1α expression.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Jing X,Du T,Yang X,Zhang W,Wang G,Liu X,Li T,Jiang Z

    更新日期:2020-12-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Vandewalle A,Rafestin-Oblin ME,Hagege J,Géniteau-Legendre M,Cassingéna R,Ronco PM

    更新日期:1993-08-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yan D,Liu W,Liu Y,Luo M

    更新日期:2019-12-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lee MY,Lu A,Gudas LJ

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    authors: De Cristan G,Morbidelli L,Alessandri G,Ziche M,Cappa AP,Gullino PM

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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    更新日期:1983-02-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Tang BL

    更新日期:2021-03-01 00:00:00

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    journal_title:Journal of cellular physiology

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    authors: Mammoto T,Higashiyama S,Mukai M,Mammoto A,Ayaki M,Mashimo T,Hayashi Y,Kishi Y,Nakamura H,Akedo H

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    journal_title:Journal of cellular physiology

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    authors: Dornadula S,Thiruppathi S,Palanisamy R,Umapathy D,Suzuki T,K Mohanram R

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Engström W,Zetterberg A

    更新日期:1984-08-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chevessier F,Hantaï D,Verdière-Sahuqué M

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Xu W,Hou D,Jiang X,Lu Z,Guo T,Liu Y,Wang D,Zen K,Yu B,Zhang CY

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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    更新日期:1990-01-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lallier R,Trudel M,Brailovsky CA,Nigam VN

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Xi G,Maile LA,Yoo SE,Clemmons DR

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Prado GN,Taylor L,Zhou X,Ricupero D,Mierke DF,Polgar P

    更新日期:2002-12-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Hassanshahi A,Hassanshahi M,Khabbazi S,Hosseini-Khah Z,Peymanfar Y,Ghalamkari S,Su YW,Xian CJ

    更新日期:2019-06-01 00:00:00