Direct evidence for nuclear and cytoplasmic colocalization of proteasomes (multiprotease complexes) in liver.


:Subcellular localization of the large multicatalytic protease complexes called proteasomes, which have been found in soluble fractions of various cells, was examined by biochemical, immunological, and immunohistological methods. Rat liver nuclei, purified by two different procedures, showed high activities for degrading [3H]methylcasein and various fluorogenic oligopeptides with neutral and weakly alkaline pH optima. On gel filtration, all of these peptidase activities were recovered in a single peak with the unusually large molecular weight of about 600,000. Properties of the proteolytic activity in crude extracts of the nucleus and the cytoplasm were very similar. Immunoelectrophoretic blot analysis showed the presence of appreciable concentrations of proteasomes with similar immunoreactivity in isolated nuclear and cytosolic fractions. Moreover, immunohistochemical staining of human liver showed that proteasomes were predominantly localized in the nuclear matrix but also were present diffusely in the cytoplasm of hepatocytes. These findings indicate the nuclear and cytoplasmic colocalization of proteasomes.


J Cell Physiol


Tanaka K,Kumatori A,Ii K,Ichihara A




Has Abstract


1989-04-01 00:00:00












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    journal_title:Journal of cellular physiology

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    authors: Liu Y,Templeton DM

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    abstract::The poor prognosis of pancreatic cancer patients using currently available therapies mandates novel therapeutics that combine anti-neoplastic potency with toxicity-minimizing cancer specificity. Employing an overlapping pathway screen to identify genes exhibiting coordinated expression as a consequence of terminal cel...

    journal_title:Journal of cellular physiology

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    authors: Chan I,Lebedeva IV,Su ZZ,Sarkar D,Valerie K,Fisher PB

    更新日期:2008-05-01 00:00:00

  • Cell cycle dependent alterations of chromatin structure in situ as revealed by the accessibility of the nuclear protein AF-2 to monoclonal antibodies.

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    journal_title:Journal of cellular physiology

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    authors: Pfeffer U,Di Vinci A,Geido E,Vidali G,Giaretti W

    更新日期:1991-12-01 00:00:00

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    abstract::Hypoxia promotes keratinocyte migration on wound bed connective tissues and is a profound biological signal that transforms a basal keratinocyte, destined to differentiate, into a motile cell that is essential for re-epithelialization. In this study, we examined the effect of hypoxia on keratinocyte-derived collagenas...

    journal_title:Journal of cellular physiology

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    authors: O'Toole EA,van Koningsveld R,Chen M,Woodley DT

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    authors: De Falco G,Leucci E,Onnis A,Bellan C,Tigli C,Wirths S,Cerino G,Cocco M,Crupi D,De Luca A,Lanzavecchia A,Tosi P,Leoncini L,Giordano A

    更新日期:2008-04-01 00:00:00

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    journal_title:Journal of cellular physiology

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    更新日期:2016-01-01 00:00:00

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    journal_title:Journal of cellular physiology

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    authors: Falcone DJ,McCaffrey TA,Mathew J,McAdam K,Borth W

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    pub_type: 杂志文章,评审


    authors: Shapouri-Moghaddam A,Mohammadian S,Vazini H,Taghadosi M,Esmaeili SA,Mardani F,Seifi B,Mohammadi A,Afshari JT,Sahebkar A

    更新日期:2018-09-01 00:00:00

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    journal_title:Journal of cellular physiology

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    pub_type: 杂志文章


    authors: Smith AN,Muffley LA,Bell AN,Numhom S,Hocking AM

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Barash I

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    authors: Takahashi S,Nakagawa S,Zeydel M,Bhargava G

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    authors: Kutner S,Ginsburg H,Cabantchik ZI

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    authors: Vacanti V,Kong E,Suzuki G,Sato K,Canty JM,Lee T

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    journal_title:Journal of cellular physiology

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    authors: Gao W,Cui H,Li Q,Zhong H,Yu J,Li P,He X

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yeh YC,Wang CZ,Tang MJ

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bashford CL,Alder GM,Gray MA,Micklem KJ,Taylor CC,Turek PJ,Pasternak CA

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Ashrafizadeh M,Ahmadi Z,Farkhondeh T,Samarghandian S

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  • Expression of malignancy in hybrids between normal and malignant cells.

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    pub_type: 杂志文章


    authors: Croce CM,Barrick J,Linnenbach A,Koprowski H

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Vecchié A,Bonaventura A,Toldo S,Dagna L,Dinarello CA,Abbate A

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    pub_type: 杂志文章


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