Abstract:
:Vitamin D3 metabolites regulate the differentiation of chondrocytes isolated from the growth zone or resting zone of rat costochondral cartilage. Since some of the direct membrane effects of vitamin D metabolites are nongenomic, we hypothesized that protein kinase C (PKC) plays a role in signal transduction for these chondrocyte differentiation factors and that the regulation of PKC by the vitamin D metabolites is cell maturation dependent. Confluent, fourth passage cultures of growth zone and resting zone chondrocytes were treated with vitamin D3 metabolites for up to 24 h, lysed, and cell extracts assayed for kinase activity using a specific PKC substrate peptide. The addition of 1,25-(OH)2D3 to growth zone cell cultures resulted in a rapid dose-dependent stimulation of PKC, significant at 10(-9)-10(-7) M, beginning at 3 min and sustained until 90 min; 1,25-(OH)2D3 had no effect on PKC activity in resting zone chondrocyte cultures. The addition of 24,25-(OH)2D3 to resting zone cultures showed a slower PKC activation, with significant stimulation seen at 90-360 min for 10(-8)-10(-7) M 24,25-(OH)2D3. However, 24,25-(OH)2D3 had no effect on PKC activity in growth zone cell cultures at all times and concentrations examined. The specificity of PKC stimulation by the vitamin D3 metabolites was verified using a specific pseudosubstrate region peptide inhibitor, which reduced PKC activity when included in the reaction mixture. Pretreatment of the cultures with U73, 122, a phospholipase C inhibitor, decreased 1,25-(OH)2D3-stimulated PKC activity but had no effect upon 24,25-(OH)2D3-induced activity. The tyrosine kinase inhibitor, genistein, did not inhibit the PKC response in either vitamin D3 metabolites-treated culture. Neither actinomycin D nor cycloheximide affected 1,25-(OH)2D3-induced PKC activity in growth zone chondrocyte cultures, while both compounds inhibited 24,25-(OH)2D3-induced activity in resting zone chondrocyte cultures. The results of this study indicate that vitamin D metabolites stimulate PKC activity in a metabolite- and cell-maturation-specific manner. Effects of 1,25-(OH)2D3 appear to be nongenomic, whereas the effects of 24,25-(OH)2D3 probably involve a genomic mechanism.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Sylvia VL,Schwartz Z,Schuman L,Morgan RT,Mackey S,Gomez R,Boyan BDdoi
10.1002/jcp.1041570209subject
Has Abstractpub_date
1993-11-01 00:00:00pages
271-8issue
2eissn
0021-9541issn
1097-4652journal_volume
157pub_type
杂志文章abstract::While retinoids have been demonstrated to inhibit growth of many tumor cells, including SCC cells, the molecular mechanism by which retinoids suppress growth has not been elucidated. We previously found that the growth of SCC cells was significantly inhibited by all-trans-retinoic acid (all-trans-RA) treatment, and th...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10051
更新日期:2002-03-01 00:00:00
abstract::This study examined the role of agonist-induced Rho kinase (ROCK) involvement in the morphological outcome of pulmonary-derived fibroblasts. Normal human lung fibroblasts (NHLF) spontaneously differentiate into network-like structures in a two-dimensional growth factor reduced Matrigel matrix-based assay. Sphingosine ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20939
更新日期:2007-05-01 00:00:00
abstract::Hydatid cyst, the larval stage of the tapeworm Echinococcus granulosus and a causative agent of cystic echinococcosis, possesses a vast number of antigenic peptides that are constantly presented in the host immune system during infection. Here, we sought to provide more information about the cellular/humoral component...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27092
更新日期:2019-03-01 00:00:00
abstract::Lung diseases are major cause of morbidity and mortality worldwide. The progress in regenerative medicine and stem cell research in the lung are currently a fast-growing research topic that can provide solutions to these major health problems. Under normal conditions, the rate of cellular proliferation is relatively l...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.26414
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND:Elevated microsatellite alteration at selected tetranucleotide repeats (EMAST) is a type of microsatellite instability that occurs in ∼60% of colorectal cancers (CRCs) and associated with MSH3 dysfunction. A 5-fluorouracil (5-FU)-related cytotoxicity is attenuated in MSH3-deficient colon cancer cells. Report...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29249
更新日期:2020-04-01 00:00:00
abstract::The zafirlukast has been reported to be anti-inflammatory and widely used to alleviate the symptoms of asthma. However, its influence on insulin secretion in pancreatic β-cells has not been investigated. Herein, we examined the effects of zafirlukast on insulin secretion and the potential underlying mechanisms. Among ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26750
更新日期:2018-11-01 00:00:00
abstract::Pyroptosis is a form of necrotic and inflammatory programmed cell death, which could be characterized by cell swelling, pore formation on plasma membranes, and release of proinflammatory cytokines (IL-1β and IL-18). The process of pyroptosis presents as dual effects: protecting multicellular organisms from microbial i...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.29268
更新日期:2020-04-01 00:00:00
abstract::The effect of changes in muscle length on post-tetanic isometric twitch tension potentiation and myosin P-light chain phosphorylation was studied at 23 degrees C in the mouse extensor digitorum longus muscle. The length-tension relationship was determined for the same muscles after a 30 min period of quiescence and be...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041430209
更新日期:1990-05-01 00:00:00
abstract::The human neuroblastoma cell line SK-N-BE, after incubation with 10 microM retinoic acid (RA) or 20 nM phorbol 12-myristate 13-acetate (PMA), underwent biochemical and morphological signs of differentiation within 10-14 days. In parallel, SK-N-BE cells produced significantly higher amounts of nitric oxide (NO) in comp...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199801)174:1<99::AID-JCP11
更新日期:1998-01-01 00:00:00
abstract::In this study, we used a bioinformatics approach to analyze the nucleotide composition and pattern of synonymous codon usage in mitochondrial ND genes in three amphibian groups, that is, orders Anura, Caudata, and Gymnophiona to identify the commonality and the differences of codon usage as no research work was report...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30050
更新日期:2020-09-22 00:00:00
abstract::Overexpression of IGF-1 in C2C12 myoblasts causes hypertrophy when myoblasts fuse to form myotubes, a response that requires elevated intracellular calcium. We show that myoblasts contain a store-operated Ca2+ channel (SOCC) whose activity is enhanced with IGF-1 overexpression. A membrane protein, CD20, can cause Ca2+...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10347
更新日期:2003-10-01 00:00:00
abstract::It was the goal of this study to determine whether during long-term quiescence WI-38 cells gradually lose labile components which then need to be resynthesized before a stimulated cell can progress through G-1 and enter S. The metabolic and molecular status of WI-38 cells was systematically analyzed as they entered an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041420117
更新日期:1990-01-01 00:00:00
abstract::Studies were conducted to explore hormonal and nutritional factors that might be involved in the regulation of retinol-binding protein (RBP) synthesis and secretion by the liver. The studies employed primary cultures of hepatocytes from normal rats. When cells were cultured in Dulbecco's modified Eagle's medium alone,...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041300103
更新日期:1987-01-01 00:00:00
abstract::The present study was to investigate the effects and action mechanisms of digoxin and ouabain on steroidogenesis in human adrenocortical NCI-H295 cells. Administration of digoxin or ouabain for 24 h decreased the basal and angiotensin II (Ang II)-stimulated release of aldosterone by NCI-H295 cells. The conversions of ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20415
更新日期:2005-12-01 00:00:00
abstract::The purpose of this study was to evaluate the anti-head and neck squamous cell carcinoma (anti-HNSCC) cell activity by C6 ceramide and multikinase inhibitor PKC412. Experiments were performed on HNSCC cell lines (SQ20B and SCC-9) and primary human oral carcinoma cells. Results showed that PKC412 inhibited HNSCC cell p...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26831
更新日期:2018-12-01 00:00:00
abstract:BACKGROUND:In this study, we aimed to explore the effects of GAS5 single-nucleotide polymorphisms (SNPs) on GAS5 expression. And the signaling pathways underlying the function of GAS5 during the pathogenesis of prostate cancer (PC) were clarified. MATERIALS AND METHODS:Patients with PC were recruited and grouped accor...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27561
更新日期:2019-06-01 00:00:00
abstract::With the long-range goals of elucidating the biochemical genetics and the phenotypic expression, both in vitro and in vivo, of mutator strains of mammalian cells, we have isolated and partially characterized a series of drug-resistant mutants from a subline a feeder-dependent parental mouse teratocarcinoma line (PSA-1...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041150103
更新日期:1983-04-01 00:00:00
abstract::S100B, a Ca(2+)-modulated protein with both intracellular and extracellular regulatory roles, is most abundant in astrocytes, is expressed in various amounts in several non-nervous cells and is also found in normal serum. Astrocytes secrete S100B, and extracellular S100B exerts trophic and toxic effects on neurons dep...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10462
更新日期:2004-05-01 00:00:00
abstract::These studies describe a liquid suspension culture system for normal myeloid cells derived from human foetal liver. A simple one-step fractionation procedure was employed to obtain a cell population capable of expanding into all stages of myeloid differentiation, including committed myeloid progenitor cells (GM-CFC). ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041190213
更新日期:1984-05-01 00:00:00
abstract::MCOLN1 encodes mucolipin-1 (TRPML1), a member of the transient receptor potential TRPML subfamily of channel proteins. Mutations in MCOLN1 cause mucolipidosis-type IV (MLIV), a lysosomal storage disorder characterized by severe neurologic, ophthalmologic, and gastrointestinal abnormalities. Along with TRPML1, there ar...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21956
更新日期:2010-02-01 00:00:00
abstract::The molecular mechanism by which thyroid hormones exert their effects on cell growth is still unknown. In this study, we used chick embryo hepatocytes at different stages of development as a model to investigate the effect of the two thyroid hormones, T3 and T4, and of their metabolite T2, on the control of cell proli...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20060
更新日期:2004-11-01 00:00:00
abstract::Osteoclasts are responsible for bone resorption during the process of bone remodeling. Increased osteoclast numbers and bone resorption activity are the main factors contributing to bone loss-related diseases such as osteoporosis. Therefore, modulating the formation and function of osteoclasts is critical for the effe...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26893
更新日期:2018-01-01 00:00:00
abstract::The aim of this study was to determine the effects and molecular mechanism of blue light emitting diode (LED) in tumor cells. A migration and invasion assay for the metastatic behavior of mouse colon cancer CT-26 and human fibrosarcoma HT-1080 cells was performed. Cancer cell migration-related proteins were identified...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25805
更新日期:2017-12-01 00:00:00
abstract::We report the isolation of a bovine pituitary growth factor (MGF) for a rat mammary carcinoma cell line, 64-24, which was isolated from a highly hormone-dependent mammary tumor. The MGF has been partially purified by a series of Diaflo ultrafiltration membrane sievings, isoelectric focusing and Sephadex columns. The M...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040930313
更新日期:1977-12-01 00:00:00
abstract::Rat thyroid follicles in both the normal and the inverted configuration have been cultured in suspension. It has been found that normal follicles (i.e., those having a structural organization similar to the follicles in vivo) express most functional properties of the thyroid gland. They trap iodide, synthesize, secret...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041260113
更新日期:1986-01-01 00:00:00
abstract::Hypoxia promotes keratinocyte migration on wound bed connective tissues and is a profound biological signal that transforms a basal keratinocyte, destined to differentiate, into a motile cell that is essential for re-epithelialization. In this study, we examined the effect of hypoxia on keratinocyte-derived collagenas...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21160
更新日期:2008-01-01 00:00:00
abstract::B cell activating factor from the TNF family (BAFF) stimulates B-cell proliferation and survival, but excessive BAFF promotes the development of aggressive B cells leading to malignant and autoimmune diseases. Recently, we have reported that rapamycin, a macrocyclic lactone, attenuates human soluble BAFF (hsBAFF)-stim...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25913
更新日期:2018-01-01 00:00:00
abstract::The endogenous thiols (PSH, protein sulfhydryls; NPSH, nonprotein sulfhydryls; and GSH, glutathione) were measured in the 66 and 67 murine carcinoma cells growing under different physiological conditions in vitro (e.g., proliferation, P; nutrient-deprived quiescence QI; and QI cells stimulated by refeeding the monolay...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041320121
更新日期:1987-07-01 00:00:00
abstract::This study is carried out to elucidate the role of long noncoding RNAs (lncRNAs) MT1JP in proliferation, invasion, migration, and apoptosis of glioma cells through the regulation of PTEN/Akt signaling pathway. The expression of MT1JP in 80 normal brain tissues and 138 glioma tissues, as well as glioma cell lines, was ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28553
更新日期:2019-11-01 00:00:00
abstract::Osteoclasts and macrophages share progenitors that must receive decisive lineage signals driving them into their respective differentiation routes. Macrophage colony stimulation factor M-CSF is a common factor; bone is likely the stimulus for osteoclast differentiation. To elucidate the effect of both, shared mouse bo...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24702
更新日期:2015-01-01 00:00:00