Abstract:
:A method for killing dividing cells (Puck and Kao, '67) was adapted for the elimination of dividing heart muscle cells (myoblasts) in cultures. We have used this method to demonstrate their presence and to estimate their number as well as the number of nondividing heart muscle cells (myocytes) in the neo-natal rat heart. Cells were cultivated in BUdR (5-bromodeoxyuridine) 10(-4) M for 3 days and then irradiated with long UV light. The selective elimination of dividing cells led to a loss of myosin Ca2+-activated ATPase in the cultures. This indicates the presence of dividing cells which contain myosin. The percent of ATPase left after irradiation was 32% of the control in cultures derived from 1-day postnatal rats and 48% in cultures from 4-day postnatal rats. This reflects an in vivo shift of myoblasts to myocytes in the muscle cell population as the rat ages.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Masse MJ,Harary Idoi
10.1002/jcp.1041050203subject
Has Abstractpub_date
1980-11-01 00:00:00pages
197-207issue
2eissn
0021-9541issn
1097-4652journal_volume
105pub_type
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