Abstract:
:Growth-contingent alterations in potassium and sodium fluxes, ouabain binding, and potassium ion content were examined following serum stimulation of quiescent, density-inhibited chicken embryo fibroblasts. Serum stimulation resulted in very rapid 1.5- to 1.8-fold increases in ouabain-sensitive potassium influx and lesser 1.4- to 1.5-fold increases in potassium efflux and sodium influx. Potassium influx stimulation was maximal after addition of 5-20% calf serum and was unaffected by cycloheximide inhibition of protein synthesis. Reflecting the slightly greater stimulation of potassium influx versus potassium efflux, potassium ion levels were 10-15% higher in serum-stimulated compared to unstimulated cells. Specific ouabain binding levels in stimulated and unstimulated control cells were initially similar, however, by four hours after stimulation a 40-50% increase in specific ouabain binding was observed. Incubation with ouabain was found also to inhibit later serum-stimulated hexose uptake and thymidine incorporation; this blockage may be consequence of subnormal potassium levels rather than ouabain inhibition of the serum-stimulated potassium influx.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Johnson MA,Weber MJdoi
10.1002/jcp.1041030222subject
Has Abstractpub_date
1980-05-01 00:00:00pages
363-70issue
2eissn
0021-9541issn
1097-4652journal_volume
103pub_type
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