Increased P-glycoprotein messenger RNA stability in rat liver tumors in vivo.


:P-glycoproteins (Pgp) are comprised of a small family of plasma membrane proteins whose abundance in cultured cells is often associated with the multidrug resistance phenotype. Overexpression of Pgp has been observed in many types of human cancers, but the molecular basis for this overexpression has not been established. We have used primary monolayer cultures of adult rat hepatocytes and a stepwise model of rat liver carcinogenesis to study the regulation of Pgp gene expression. We observed a marked overexpression of Pgp, specifically the class II Pgp, in both systems. In addition, we observed that a number of unrelated genes including alpha-tubulin, beta-actin, gamma-actin, cytokeratin 8, cytokeratin 18, and c-myc are overexpressed in cultured hepatocytes, and they are also overexpressed during liver carcinogenesis and in transplantable tumors. Nuclear run-on assays showed no increase in the transcriptional activity of Pgp genes in transplantable liver tumors compared to normal liver. Studies of in vivo mRNA stability, however, revealed that all three Pgp mRNAs were relatively stable in transplantable liver tumors (t(1/2) > 12 h), in contrast to what was found in normal liver (t(1/2) < 2 h). In addition, mRNA for several other genes, including alpha-tubulin, c-myc, and cyclin D1, all appear to be stabilized in the tumors. These findings suggest that the overexpression of Pgp genes in rat liver tumors may be the result of a mechanism involving stabilization of a diverse group of mRNAs.


J Cell Physiol


Lee CH,Bradley G,Ling V




Has Abstract


1998-10-01 00:00:00














  • Mutations in GAS5 affect the transformation from benign prostate proliferation to aggressive prostate cancer by affecting the transcription efficiency of GAS5.

    abstract:BACKGROUND:In this study, we aimed to explore the effects of GAS5 single-nucleotide polymorphisms (SNPs) on GAS5 expression. And the signaling pathways underlying the function of GAS5 during the pathogenesis of prostate cancer (PC) were clarified. MATERIALS AND METHODS:Patients with PC were recruited and grouped accor...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhu L,Zhu Q,Wen H,Huang X,Zheng G

    更新日期:2019-06-01 00:00:00

  • Ligand concentration is a driver of divergent signaling and pleiotropic cellular responses to FGF.

    abstract::Fibroblast growth factors (FGFs) are soluble ligands important for embryonic patterning, limb and brain development, and stem cell proliferation. They activate specific receptors (FGFR) to elicit changes in gene expression and cellular responses such as proliferation, differentiation, and survival, but the extent to w...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Garcia-Maya M,Anderson AA,Kendal CE,Kenny AV,Edwards-Ingram LC,Holladay A,Saffell JL

    更新日期:2006-02-01 00:00:00

  • CCL18-dependent translocation of AMAP1 is critical for epithelial to mesenchymal transition in breast cancer.

    abstract::AMAP1 was a GTPase-activating protein that regulates cytoskeletal structures in focal adhesions, circular dorsal ruffles, and promote cell differentiation in tumor cells. But the activation and function of AMAP1 in breast cancer remain largely unexplored. Here we show that AMAP1 was phosphorylated and translocated to ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Li H,Zhang D,Yu J,Liu H,Chen Z,Zhong H,Wan Y

    更新日期:2018-04-01 00:00:00

  • Evidences of a role for eukaryotic translation initiation factor 5A (eIF5A) in mouse embryogenesis and cell differentiation.

    abstract::Eukaryotic translation initiation factor 5A (eIF5A) has a unique character: the presence of an unusual amino acid, hypusine, which is formed by post-translational modifications. Even before the identification of hypusination in eIF5A, the correlation between hypusine formation and protein synthesis, shifting cell prol...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Parreiras-e-Silva LT,Luchessi AD,Reis RI,Oliver C,Jamur MC,Ramos RG,Oliveira EB,Curi R,Costa-Neto CM

    更新日期:2010-11-01 00:00:00

  • Angiotensin type 1 receptor autoantibody from preeclamptic patients induces human fetoplacental vasoconstriction.

    abstract::Increased vascular resistance in the fetoplacental circulation is a characteristic of preeclampsia. However, the potential molecular mechanisms of this condition remain obscure. The current study aimed to determine the direct effect of the peptide antigen corresponding to the second extracellular loop of the angiotens...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhang S,Zheng R,Yang L,Zhang X,Zuo L,Yang X,Bai K,Song L,Tian J,Yang J,Liu H

    更新日期:2013-01-01 00:00:00

  • Signal transducer and activator of transcription 3 mediates apoptosis inhibition through reducing mitochondrial ROS and activating Bcl-2 in gemcitabine-resistant lung cancer A549 cells.

    abstract::Lung cancer is a leading cause of cancer-related death worldwide. In this study, we used lung adenocarcinoma cells as a model, as lung adenocarcinoma has the highest mortality rate among all lung cancers. For the past few years, medical treatments or lung cancer have been limited because of chemotherapy resistance. Th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Liu JS,Yeh CA,Huang IC,Huang GY,Chiu CH,Mahalakshmi B,Wen SY,Huang CY,Kuo WW

    更新日期:2020-12-07 00:00:00

  • Prognostic relevance of miR-137 and its liver microenvironment regulatory target gene AFM in hepatocellular carcinoma.

    abstract::MiR-137 has been identified as potential hepatocellular carcinoma (HCC) prognostic biomarkers. Highly relevant HCC prognostic biomarkers may be derived from combinations of miR-137 with its target genes involved in the regulation of liver microenvironment. This study aimed at the discovery of such a combination with i...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wei Q,Zhao L,Jiang L,Bi J,Yu Z,Zhao L,Song X,Sun M,Chen Y,Wei M

    更新日期:2019-07-01 00:00:00

  • Effects of Sex Hormones on Ocular Surface Epithelia: Lessons Learned From Polycystic Ovary Syndrome.

    abstract::Polycystic ovary syndrome (PCOS) is the most common endocrine abnormality in women of reproductive age. Although its clinical consequences have been known for a long time to extend beyond the reproductive system, with type-2 diabetes and obesity being the most common, the involvement of the ocular surface in PCOS has ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Mantelli F,Moretti C,Macchi I,Massaro-Giordano G,Cozzupoli GM,Lambiase A,Bonini S

    更新日期:2016-05-01 00:00:00

  • Interaction of endothelial cells with a laminin A chain peptide (SIKVAV) in vitro and induction of angiogenic behavior in vivo.

    abstract::Endothelial cells are known to bind to laminin, and two peptides derived from the laminin A (CTFALRGDNP) and B1 (CDPGYIGSR) chains block the capillary-like tube formation on a laminin-rich basement membrane matrix, Matrigel. In the present study, we have used various in vitro and in vivo assays to investigate the angi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Grant DS,Kinsella JL,Fridman R,Auerbach R,Piasecki BA,Yamada Y,Zain M,Kleinman HK

    更新日期:1992-12-01 00:00:00

  • A new apoptotic pathway for the complement factor B-derived fragment Bb.

    abstract::Apoptosis is involved in both the cellular and humoral immune system destroying tumors. An apoptosis-inducing factor from HL-60 myeloid leukemia cells was obtained, purified, and sequenced. The protein found has been identified as a human complement factor B-derived fragment Bb, although it is known that factor B is a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Uwai M,Terui Y,Mishima Y,Tomizuka H,Ikeda M,Itoh T,Mori M,Ueda M,Inoue R,Yamada M,Hayasawa H,Horiuchi T,Niho Y,Matsumoto M,Ishizaka Y,Ikeda K,Ozawa K,Hatake K

    更新日期:2000-11-01 00:00:00

  • Melatonin inhibits Müller cell activation and pro-inflammatory cytokine production via upregulating the MEG3/miR-204/Sirt1 axis in experimental diabetic retinopathy.

    abstract::Diabetic retinopathy (DR) is the most common ocular complication caused by diabetes mellitus and is the main cause of visual impairment in working-age people. Reactive gliosis and pro-inflammatory cytokine production by Müller cells contribute to the progression of DR. Melatonin is a strong anti-inflammatory hormone, ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tu Y,Zhu M,Wang Z,Wang K,Chen L,Liu W,Shi Q,Zhao Q,Sun Y,Wang X,Song E,Liu X

    更新日期:2020-11-01 00:00:00

  • Synthesis, characterization, and in vitro evaluation of the starch-based α-amylase responsive hydrogels.

    abstract::Controlled-release drug delivery systems are promising platforms in medicine. Among various types of material in drug delivery, hydrogels are interesting ones. They are water-soluble and tissue compatible polymers with a high capacity to carry and release drugs in a controllable manner. In this study, we introduce the...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Davoodi-Monfared P,Akbari-Birgani S,Mohammadi S,Kazemi F,Nikfarjam N,Nikbakht M,Mousavi SA

    更新日期:2020-11-05 00:00:00

  • The differential palmitoylation states of N-Ras and H-Ras determine their distinct Golgi subcompartment localizations.

    abstract::Despite a high degree of structural homology and shared exchange factors, effectors and GTPase activating proteins, a large body of evidence suggests functional heterogeneity among Ras isoforms. One aspect of Ras biology that may explain this heterogeneity is the differential subcellular localizations driven by the C-...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lynch SJ,Snitkin H,Gumper I,Philips MR,Sabatini D,Pellicer A

    更新日期:2015-03-01 00:00:00

  • Down-modulation of EGF receptors in cells transformed by the src oncogene.

    abstract::The effects of src oncogene expression on epidermal growth factor (EGF) receptors have been investigated in mouse 3T3 and rat-1 fibroblasts. Transformation of both cell types with src resulted in marked reductions in cellular EGF receptor levels, as assayed by either 125I-EGF binding or immunoprecipitation of receptor...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wasilenko WJ,Shawver LK,Weber MJ

    更新日期:1987-06-01 00:00:00

  • Evidence that density-dependent growth arrest is a two-stage process in WI-38 cells.

    abstract::It was the goal of this study to determine whether during long-term quiescence WI-38 cells gradually lose labile components which then need to be resynthesized before a stimulated cell can progress through G-1 and enter S. The metabolic and molecular status of WI-38 cells was systematically analyzed as they entered an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Owen TA,Carter R,Whitman MM,Soprano DR,Soprano KJ

    更新日期:1990-01-01 00:00:00

  • Pectolinarigenin prevents bone loss in ovariectomized mice and inhibits RANKL-induced osteoclastogenesis via blocking activation of MAPK and NFATc1 signaling.

    abstract::Osteoporosis (OP) is a metabolic disease caused by multiple factors, which is characterized by a reduction of bone mass per unit volume and destruction of bone microstructure. Aberrant osteoclast function is the main cause of OP, therefore, regulating the differentiation and function of osteoclast is one of the treatm...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Xiao Y,Li K,Wang Z,Fu F,Shao S,Song F,Zhao J,Chen W,Liu Q,Xu J

    更新日期:2019-08-01 00:00:00

  • The non-genomic effects on Na+/H+-exchange 1 by progesterone and 20alpha-hydroxyprogesterone in human T cells.

    abstract::Progesterone is an endogenous immunomodulator and can suppress T-cell activation during pregnancy. We have previously shown that the non-genomic effects of progesterone, especially acidification, are exerted via plasma membrane sites and suppress cellular genomic responses to mitogens. This study aimed to show that ac...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chien EJ,Liao CF,Chang CP,Pu HF,Lu LM,Shie MC,Hsieh DJ,Hsu MT

    更新日期:2007-05-01 00:00:00

  • miR-335-5p induces insulin resistance and pancreatic islet β-cell secretion in gestational diabetes mellitus mice through VASH1-mediated TGF-β signaling pathway.

    abstract::Multiple studies have reported different methods in treating gestational diabetes mellitus (GDM); however, the relationship between miR-335-5p and GDM still remains unclear. Here, this study explores the effect of miR-335-5p on insulin resistance and pancreatic islet β-cell secretion via activation of the TGFβ signali...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tang XW,Qin QX

    更新日期:2019-05-01 00:00:00

  • SIRT1 interacts with p73 and suppresses p73-dependent transcriptional activity.

    abstract::The tumor suppressor p53-related p73 shares significant amino-acid sequence identity with p53. Like p53, p73 recognizes canonical p53 DNA-binding sites and activates p53-responsive target genes and induces apoptosis. Moreover, SIRT1 binds to p53 while repressing the expression of their target genes. Here, we report th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dai JM,Wang ZY,Sun DC,Lin RX,Wang SQ

    更新日期:2007-01-01 00:00:00

  • Flt-1, but not Flk-1 mediates hyperpermeability through activation of the PI3-K/Akt pathway.

    abstract::Vascular endothelial growth factor (VEGF), a potent mediator of endothelial proliferation and migration, has an important role also in brain edema formation during hypoxia and ischemia. VEGF binds to the tyrosine kinase receptors Flt-1 and Flk-1. Yet, their relative importance for hypoxia-induced hyperpermeability is ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Vogel C,Bauer A,Wiesnet M,Preissner KT,Schaper W,Marti HH,Fischer S

    更新日期:2007-07-01 00:00:00

  • Growth inhibitory activity in extracts from human fibroblasts.

    abstract::Indirect evidence for the presence of a growth inhibitor in normal human fibroblasts has been obtained previously; the inhibitory activity has been found associated with crude cell extracts, but the molecule responsible for the growth inhibition has never been isolated. We have isolated a glycopeptide fraction from hu...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Macieira-Coelho A,Söderberg A

    更新日期:1993-01-01 00:00:00

  • Regulation of albumin expression in fetal rat hepatocytes cultured under proliferative conditions: role of epidermal growth factor and hormones.

    abstract::Sustained production of plasma proteins, notably albumin, is a reliable indicator of the differentiated state of hepatocytes. In this work, we have developed a fetal hepatocyte culture system where studying the regulation of albumin expression in proliferating liver cells. Our results show that under proliferative con...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: de Juan C,Benito M,Fabregat I

    更新日期:1992-07-01 00:00:00

  • The Multifaceted Roles of Primary Cilia in the Regulation of Stem Cell Properties and Functions.

    abstract::Stem cells are a unique class of cells that are capable of self-renewal and differentiation into multiple lineages. An increasing number of studies have suggested that both embryonic and adult stem cells possess primary cilia, antenna-like structures protruding from cell surfaces that are critical for sensing and tran...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Lyu R,Zhou J

    更新日期:2017-05-01 00:00:00

  • Profiling of retinoid mediated gene expression in synchronized human SCC cells using Atlas human cDNA expression arrays.

    abstract::While retinoids have been demonstrated to inhibit growth of many tumor cells, including SCC cells, the molecular mechanism by which retinoids suppress growth has not been elucidated. We previously found that the growth of SCC cells was significantly inhibited by all-trans-retinoic acid (all-trans-RA) treatment, and th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Le Q,Soprano DR,Soprano KJ

    更新日期:2002-03-01 00:00:00

  • A new insight into thymosin β4, a promising therapeutic approach for neurodegenerative disorders.

    abstract::Thymosin β4 (Tβ4), a G-actin-sequestering secreted peptide, improves neurovascular remodeling and central nervous system plasticity, which leads to neurological recovery in many neurological diseases. Inflammatory response adjustment and tissue inflammation consequences from neurological injury are vital for neurologi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Shomali N,Baradaran B,Deljavanghodrati M,Akbari M,Hemmatzadeh M,Mohammadi H,Jang Y,Xu H,Sandoghchian Shotorbani S

    更新日期:2020-04-01 00:00:00

  • Entactin promotes adhesion and long-term maintenance of cultured regenerated skeletal myotubes.

    abstract::The basal lamina protein, laminin, has been shown to promote migration and proliferation of cultured skeletal myoblasts, resulting in increased myotube formation. However, skeletal myotubes adhere poorly to a laminin substrate, and long-term cultures of skeletal myotubes on laminin have not been achieved. We have foun...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Funanage VL,Smith SM,Minnich MA

    更新日期:1992-02-01 00:00:00

  • Interplay between microRNAs and Wnt, transforming growth factor-β, and bone morphogenic protein signaling pathways promote osteoblastic differentiation of mesenchymal stem cells.

    abstract::Osteoblasts are terminally differentiated cells with mesenchymal origins, known to possess pivotal roles in sustaining bone microstructure and homeostasis. These cells are implicated in the pathophysiology of various bone disorders, especially osteoporosis. Over the last few decades, strategies to impede bone resorpti...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Aslani S,Abhari A,Sakhinia E,Sanajou D,Rajabi H,Rahimzadeh S

    更新日期:2019-06-01 00:00:00

  • Regulation of mesangial cell Na+/Ca2+ exchanger isoforms.

    abstract::An isoform of the Na(+)/Ca(2+) exchanger (SDNCX1.10) was cloned from mesangial cells of Sprague-Dawley rat. Regulation of this isoform was compared to two other clones that were derived from the Dahl/Rapp salt sensitive (SNCX) and salt resistant rat (RNCX). All isoforms differ at the alternative splice site and at ami...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Williams I,Williams C,Siroky B,Bates E,Kovacs G,Peti-Peterdi J,Unlap MT,Bell PD

    更新日期:2004-05-01 00:00:00

  • Direct effects of wheat germ agglutinin on inositol phosphate formation and cytosolic-free calcium level in intestine 407 cells.

    abstract::The interaction between dietary lectins, especially wheat germ agglutinin (WGA), and intestinal cells has been implicated in the pathogenesis of celiac disease. The present study was undertaken to investigate the immediate effects following such an interaction. Direct WGA-stimulation of Intestine 407 cells leads to an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sjölander A

    更新日期:1988-03-01 00:00:00

  • Interleukin-6 (IL-6) and low O(2) concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (pre-CFC).

    abstract::Low O(2) concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both stem cells and committed progenitors at 20% O(2), we studied its effects in cultures at 1% O(2). The pre-CFC activity in Lin- population of mouse bone marrow was analyzed...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kovacević-Filipović M,Petakov M,Hermitte F,Debeissat C,Krstić A,Jovcić G,Bugarski D,Lafarge X,Milenković P,Praloran V,Ivanović Z

    更新日期:2007-07-01 00:00:00