Abstract：
:We have investigated the characteristics of a transport system in HeLa cells, which turned out to be very similar to a previously described Na+, K+, 2Cl- -cotransport system. For further understanding about the physiological role of the cotransporter, we have mutagenized HeLa cells and selected progeny cells for growth in low potassium (0.2 mM) medium. The selected HeLa cells (LK1) exhibited alterations in the Na+,K+,2Cl- -cotransport system. LK1 cells showed a remarkable reduction of 86Rb+ efflux via the cotransporter when compared to the parental HeLa cells. In contrast, bumetanide-sensitive potassium influx, measured by 86Rb+ uptake, was increased in the LK1 cells (increase in Vmax). Km values of the cotransporter in HeLa cells and LK1 mutants revealed similar properties for 86Rb+ and 22Na+ uptake. In addition, (3H)-bumetanide binding studies were carried out on intact HeLa cells; 1.7 pmol/mg protein (3H)-bumetanide was specifically bound to HeLa parental cells, which could be calculated to a number of 103,000 binding sites/cell. LK1 cells present, 1.44 pmol/mg protein, specifically bound (3H)-bumetanide and, respectively, 137,000 binding sites/cell. The LK1 cells also exhibited an increase in the number of (3H)-ouabain binding sites as well as an increase in the activity of the Na+,K+-ATPase, expressed as a function of ouabain-sensitive 86Rb+ uptake. Furthermore, LK1 cells were different in the concentrations of intracellular Na+ (increases) and K+ (decreases) when compared to the HeLa parental cells. When grown in low K+ medium (0.2 mM K+), protein content and cell volume were increased in the LK1 cells, while the DNA content was not significantly different between both cell lines.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Kort JJ,Koch Gdoi
10.1002/jcp.1041410126subject
Has Abstractpub_date
1989-10-01 00:00:00pages
181-90issue
1eissn
0021-9541issn
1097-4652journal_volume
141pub_type
杂志文章abstract：:The peroxisome proliferation-activated receptor gamma (PPARgamma) is mainly expressed in the adipose tissue and integrates the control of energy, lipid, and glucose homeostasis. The present study, by means of RT-PCR, Western blot, and immunofluorescence techniques, demonstrates that human sperm express the PPARgamma. ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20807
更新日期：2006-12-01 00:00:00
abstract：:Ebp1, an ErbB-3 binding protein, inhibits the proliferation and induces the differentiation of human breast cancer cells. The mechanisms of these effects are unknown. Rb, the product of the retinoblastoma gene, is an important modulator of cell cycle progression and cellular differentiation. We report that Rb is a bin...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1075
更新日期：2001-05-01 00:00:00
abstract：:MicroRNAs (miRNAs or miRs) are small noncoding RNAs that play critical roles in muscle cell proliferation and differentiation via post-transcriptional regulation of gene expression. Here, based on our previous high-throughput sequencing results, we evaluated miRNA-499 (miR-499) functions during myoblast proliferation ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：2019-03-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041200215
更新日期：1984-08-01 00:00:00
abstract：:Circular RNAs (circRNAs) play critical roles in signal transduction during cell proliferation, differentiation, and apoptosis in a posttranscriptional manner. Recently, circRNAs have been proved to be a large class of animal RNAs with regulatory potency. However, whether circRNAs can respond to mechanical force (MF) a...
journal_title：Journal of cellular physiology
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更新日期：2019-07-01 00:00:00
abstract：:Receptors for urokinase (uPA) and plasminogen provide a mechanism to direct the cellular activation of plasminogen. The regulation of these receptors is important for several macrophage functions. In these studies, the effect of transforming growth factor-beta 1 (TGF-beta 1) on uPA, uPA receptor, and plasminogen recep...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041640214
更新日期：1995-08-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041000107
更新日期：1979-07-01 00:00:00
abstract：:The mechanism of zinc (Zn) uptake by microvillous membrane vesicles prepared from human term placenta has been studied. The uptake was complex, with two processes being identified. In the first process, uptake was rapid, reaching equilibrium within 2 min, and was temperature dependent, with a Q10 of 1.5. Equilibrium Z...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041510312
更新日期：1992-06-01 00:00:00
abstract：:Glucose and tumor necrosis factor-alpha (TNFalpha) concentrations are elevated in diabetes. Both of these factors correlate with diabetic vasculopathy and endothelial cell apoptosis, yet their combined effects have not been measured. We have previously shown that the angiogenic growth factor fibroblast growth factor-2...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21476
更新日期：2008-10-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199602)166:2<396::AID-JCP1
更新日期：1996-02-01 00:00:00
abstract：:Thioredoxin reductase (TrxR) is a flavoprotein that contains a C-terminal penultimate selenocysteine (Sec) and has an ability to reduce thioredoxin (Trx), which regulates the activity of NF-kappa B. To date, three TrxR isozymes, TrxR1, TrxR2, and TrxR3, have been identified. In the present study, we found that among t...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10377
更新日期：2004-01-01 00:00:00
abstract：:Heparan sulfate from the surface of a variety of mouse cells at different cell densities was examined by ion-exchange chromatography. The results of this analysis show that: (1) The heparan sulfate from new isolates of Swiss 3T3 cells transformed by SV40 virus (a DNA tumor virus) elutes from DEAE-cellulose at a lower ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040900108
更新日期：1977-01-01 00:00:00
abstract：:Circular RNAs (circRNAs) are stable and abundantly expressed in vivo but are abnormally expressed in several diseases. This study aimed to identify circRNAs acting as potential biomarkers for cardiovascular disease (CVD). Research were retrieved from the articles published by September 2018 in eight databases to compa...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,meta分析,评审
doi：10.1002/jcp.28817
更新日期：2019-12-01 00:00:00
abstract：:Calcium-activated nucleotidase 1 (CANT1, belongs to the apyrase family, is widely expressed in various organs. However, the biological function of CANT1 remains poorly explored. In this study, we aimed to investigate the expression profile and functions of CANT1 in clear cell renal cell carcinoma (ccRCC). Our data sho...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28829
更新日期：2019-12-01 00:00:00
abstract：:To clarify the manner by which erythropoietin (EP), stem cell factor (SCF), or insulin-like growth factor I (IGF-I) regulate erythropoiesis, apoptosis of human erythroid progenitor cells was investigated. Human burst-forming units-erythroid (BFU-E) partially purified from peripheral blood were cultured for 6 days to g...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041560207
更新日期：1993-08-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041390214
更新日期：1989-05-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199811)177:2<334::AID-JCP1
更新日期：1998-11-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199806)175:3<348::AID-JCP1
更新日期：1998-06-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041240213
更新日期：1985-08-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20385
更新日期：2005-10-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：1977-06-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041500114
更新日期：1992-01-01 00:00:00
abstract：:Wnt5a, a member of the noncanonical Wnt proteins, is known to play important roles in the development of various organs and in postnatal cell functions. However, little is known about the effects of Wnt5a on human periodontal ligament (PDL) cells. In this study, we examined the localization and potential function of W...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24950
更新日期：2015-11-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28270
更新日期：2019-02-11 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25198
更新日期：2016-05-01 00:00:00
abstract：:Scientists of numerous medical and life science disciplines met in Siena, Italy to discuss the latest proceedings in basic and clinical research. General models of interconnected linear and back-feeding cell-cycle control pathways provide a basis for applied molecular research. Cell-cycle determining factors essential...
journal_title：Journal of cellular physiology
pub_type：
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更新日期：2000-12-01 00:00:00
abstract：:Circular RNAs (circRNAs) have been associated with lung cancer (LC), one of the most common cancers, but the underlying molecular mechanisms of the specific correlation with LC carcinogenesis remain unveiled. Quantitative real-time polymerase chain reaction was applied to examine the level of circZNF609. LC cells were...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29481
更新日期：2021-01-01 00:00:00
abstract：:The transmural heterogeneity of the contractility in ventricular muscle has not been well-studied. Here, we investigated the calcium transient and sarcomere contraction/relaxation in the endocardial (Endo) and epicardial (Epi) myocytes. Endo and Epi myocytes were isolated from C57/BL6 mice by Langendorff perfusion. Ca...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26512
更新日期：2018-08-01 00:00:00
abstract：:Subjective cognitive decline (SCD) is frequently reported in diabetic patients. Diabetes mellitus (DM) is associated with changes in the microstructure of the brain arise in diabetic patients, including changes in gray matter volume (GMV). However, the underlying mechanisms of changes in GMV in DM patients with cognit...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.28359
更新日期：2019-08-01 00:00:00
abstract：:Tenascin-C, a six-armed extracellular matrix glycoprotein, is expressed in a temporally and spatially restricted pattern during carcinogenesis and invasion or metastasis of carcinoma cells in association with stromal-epithelial interactions. The human epidermoid carcinoma-derived cell lines, A431 and HEp-2, which do n...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041650104
更新日期：1995-10-01 00:00:00
