Abstract:
:Thioredoxin reductase (TrxR) is a flavoprotein that contains a C-terminal penultimate selenocysteine (Sec) and has an ability to reduce thioredoxin (Trx), which regulates the activity of NF-kappa B. To date, three TrxR isozymes, TrxR1, TrxR2, and TrxR3, have been identified. In the present study, we found that among these isozymes only TrxR1 was induced by tumor necrosis factor-alpha (TNF alpha) in vascular endothelial cells. Furthermore, the overexpression of TrxR1 enhanced TNF alpha-induced DNA-binding activity of NF-kappa B and NF-kappa B-dependent gene expression. The catalytic Sec residue of TrxR1, which is essential for reducing Trx, was required for this NF-kappa B activation, and aurothiomalate, an inhibitor of TrxR, suppressed TNF alpha-induced activation of NF-kappa B and the expression of NF-kappa B-targeted proinflammatory genes such as E-selectin and cyclooxygenase-2. These results suggest that TrxR1 may act as a positive regulator of NF-kappa B and may play an important role in the cellular inflammatory response.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Sakurai A,Yuasa K,Shoji Y,Himeno S,Tsujimoto M,Kunimoto M,Imura N,Hara Sdoi
10.1002/jcp.10377keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
22-30issue
1eissn
0021-9541issn
1097-4652journal_volume
198pub_type
杂志文章abstract::Erythropoietin (EPO) retards DNA breakdown characteristic of programmed cell death (apoptosis) and promotes survival in erythroid progenitor cells. The mechanism by which EPO inhibits programmed death is unknown. In the well-characterized model of glucocorticoid-treated thymocytes, activation of a Ca2+/Mg(2+)-sensitiv...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041510307
更新日期:1992-06-01 00:00:00
abstract::Glucose and tumor necrosis factor-alpha (TNFalpha) concentrations are elevated in diabetes. Both of these factors correlate with diabetic vasculopathy and endothelial cell apoptosis, yet their combined effects have not been measured. We have previously shown that the angiogenic growth factor fibroblast growth factor-2...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21476
更新日期:2008-10-01 00:00:00
abstract::Resistance to conventional chemotherapy remains a major cause of cancer relapse and cancer-related deaths. Therefore, there is an urgent need to overcome resistance barriers. To improve cancer treatment approaches, it is critical to elucidate the basic mechanisms underlying drug resistance. Increasingly, the mechanism...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.29640
更新日期:2020-10-01 00:00:00
abstract::A mutant (A204) of Chinese hamster ovary cells (CHO-K1), which is deficient in dihydroorotate (DHO) dehydrogenase (E.C. 1.3,3.1) activity, has been isolated by a replica plating procedure. The mutant does not show a requirement for exogenously added pyrimidines. Examination of intact cells shows that the mutant accumu...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040980304
更新日期:1979-03-01 00:00:00
abstract::Human peripheral blood monocytes and neutrophils secrete TGF-beta. Activation of monocytes with LPS stimulates the secretion of TGF-beta; however, the production of TGF-beta by neutrophils was not altered by treatment with LPS. The secreted TGF-beta appears to be in a fully active form since acid treatment of the cond...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041400226
更新日期:1989-08-01 00:00:00
abstract::Endothelin-1 production is increased after liver injury and the subsequent wounding response. Further, endothelin-1 has prominent effects on hepatic stellate cells (key effectors of the hepatic wounding response), including on collagen synthesis, proliferation, and expression of smooth muscle proteins. We tested the h...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10110
更新日期:2002-06-01 00:00:00
abstract::The molecular and cellular mechanisms underlying vascular remodeling are currently investigated by experimental strategies which aim to mimic the complex environmental conditions found in vivo. Some of them focus on the tubulogenic activity of dispersed endothelial cell populations, while others evaluate vascular spro...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25468
更新日期:2017-02-01 00:00:00
abstract::Members of the transforming growth factor-β (TGF-β) family of cytokines are involved in diverse physiological processes. Although TGF-β is known to play multiple roles in the mammalian central nervous system (CNS), its role in neuronal development has not been explored. We have studied the effects of TGF-β1 on the ele...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22735
更新日期:2012-01-01 00:00:00
abstract::Methylglyoxal bis-(guanylhydrazone) (mGBG) blocked the stimulation of DNA synthesis in quiescent, density-inhibited BALB/c-3T3 cells treated with platelet-derived growth factor (PDGF) and platelet-poor plasma (PPP). Competence formation produced by a transient exposure to PDGF was not effected by mGBG. In contrast, mG...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041070105
更新日期:1981-04-01 00:00:00
abstract::Numerous studies suggest that epidermal growth factor (EGF) signaling is impaired in nonproliferating senescent human diploid fibroblasts downstream of receptor binding. One possible explanation for these results is that senescent cells possess unique enzymatic activities capable of regulating functional levels of the...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041600305
更新日期:1994-09-01 00:00:00
abstract::An analysis of which component of lipopolysaccharides (LPS), the lipid or the polysaccharide (PS), is active in stimulating the murine granulopoietic system has been performed. LPS with different structures, isolated from different mutant strains of Salmonella and chemical degradation products of lipopolysaccharides h...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040870110
更新日期:1976-01-01 00:00:00
abstract::Previous experimental and clinical studies have suggested that the behavioral and pathological outcomes of spinal cord injury (SCI) are affected by the individual's age at the time of injury. However, the underlying mechanism responsible for these differences remains elusive because it is difficult to match injuries o...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22845
更新日期:2012-04-01 00:00:00
abstract::Previous studies indicate that glutamine is a critical requirement for cell proliferation in vitro. We recently showed that depletion of glutamine from the culture medium supporting growing cells significantly reduced the proportion of cells undergoing DNA synthesis. Similarly glutamine depletion significantly reduced...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041200218
更新日期:1984-08-01 00:00:00
abstract::Bone morphogenetic proteins (BMPs) play pivotal roles in bone and cartilage growth and repair. Through phenotypes of short-ear (se) mice, which have BMP-5 mutations, a role for BMP-5 in some specific aspects of skeletogenesis and cartilage growth is known. This report examines BMP-5 expression in the growth plate and ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21164
更新日期:2008-01-01 00:00:00
abstract::Obesity is a risk factor for endometrial cancer in pre- and post-menopausal women. Leptin, an adipocyte-derived hormone, in addition to the control weight homeostasis, is implicated in multiple biological actions. A recent study demonstrated that leptin promotes endometrial cancer growth and invasiveness through STAT/...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21622
更新日期:2009-03-01 00:00:00
abstract::Radiocontrast media (RCM)-induced acute kidney injury (CI-AKI) is a major clinical problem whose pathophysiology is not well understood. Direct toxic effects on renal cells, possibly mediated by reactive oxygen species, have been postulated as contributing to CI-AKI. We investigated the effect of quercetin on human re...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26213
更新日期:2018-05-01 00:00:00
abstract::The binding of [3H]tuftsin to normal and in vivo stimulated mouse peritoneal macrophage populations was studied at 22 degrees C. The [3H]tuftsin binding to thioglycollate-stimulated macrophages was shown to be rapid and saturable, with an equilibrium dissociation constant (K(D)) (calculated from a Scatchard plot) of 5...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041000106
更新日期:1979-07-01 00:00:00
abstract::CSF-1 is a hemopoietic growth factor that specifically regulates the survival, proliferation, and differentiation of mononuclear phagocytic cells. Populations of adherent bone marrow-derived macrophages (BMM) devoid of CSF-1 producing cells were used to study regulation by CSF-1 of macrophage entry into S phase. More ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041220210
更新日期:1985-02-01 00:00:00
abstract::Incubation of isolated rat soleus and EDL muscles in the presence of 10 mM leucine resulted in a decreased proteolytic rate as measured by the release of tyrosine into the incubation medium. The effect of this branched-chain amino acid (BCAA) is associated with a decreased activity of the lysosomal proteases and a dec...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/1097-4652(200009)184:3<380::AID-JCP13>3.0.
更新日期:2000-09-01 00:00:00
abstract::Endothelial cell-derived growth factor (ECDGF) is a soluble mitogen secreted in vitro by bovine aortic endothelium. ECDGF is a mixture of at least two distinct heat-stable and trypsin-sensitive mitogens. Large amounts of mitogenic activity were found in lysates prepared from cultured endothelial cells. Other nonmitoge...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041210208
更新日期:1984-11-01 00:00:00
abstract::Ménière's disease (MD) is a disorder of the inner ear characterized by an insidious onset and aspecific symptoms, such as dizziness, vertigo, tinnitus, and hearing loss, that may become very debilitating. The presence of endolymphatic hydrops is a common feature in MD patients, but the pathophysiology is still largely...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22737
更新日期:2012-01-01 00:00:00
abstract::Regulation of P-glycoprotein (Pgp) expression occurs not only at the DNA and mRNA level but also at the protein level. We showed previously that Pgp was stabilized when multidrug-resistant CH(R)C5 and SKVCR 2.0 ovarian cell lines were subjected to serum-starved or high-cell-density growth conditions, whereas Pgp turno...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(200007)184:1<17::AID-JCP2>
更新日期:2000-07-01 00:00:00
abstract::Two transport systems for neutral amino acids have been characterised in LLC-PK, cells. The first, which transports alanine in a sodium-dependent manner, also mediates alanine exchange and is perferentially inhibited by serine, cysteine, and alpha-amino-n-butyric acid. This system resembles the ASC system in Ehrlich a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041120205
更新日期:1982-08-01 00:00:00
abstract::Acute lung injury, sepsis, lung inflammation, and ventilator-induced lung injury are life-threatening conditions associated with lung vascular barrier dysfunction, which may lead to pulmonary edema. Increased levels of atrial natriuretic peptide (ANP) in lung circulation reported in these pathologies suggest its poten...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21354
更新日期:2008-06-01 00:00:00
abstract::Up-regulation of atrial natriuretic peptide (ANP) mRNA in the kidneys in several disorders has been demonstrated; however, evidence that ANP synthesized by the kidney exerts a local function has never been produced. Therefore, we investigated whether endogenous ANP could modulate high glucose-stimulated TGF-beta1, col...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21728
更新日期:2009-06-01 00:00:00
abstract::Protein sorting through vesicular compartments is highly regulated to maintain the integrity and signaling of intracellular organelles in eukaryotic cells. Sorting Nexin-2 (SNX2) is involved in protein sorting in the trans-Golgi network, endosome, and/or lysosome compartments, with loss of function leading to defect i...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20285
更新日期:2005-07-01 00:00:00
abstract::The ubiquitous α-catulin acts as a scaffold for distinct signalosomes including RhoA/ROCK; however, its function is not well understood. While α-catulin has homology to the cytoskeletal linkers α-catenin and vinculin, it appears to be functionally divergent. Here we further investigated α-catulin function in pulmonary...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25185
更新日期:2016-04-01 00:00:00
abstract::Diabetic nephropathy (DN) is one of the most serious and dangerous chronic complications caused by diabetes mellitus, and the identification and development of novel biomarkers could be beneficial for the diagnosis and prognosis of DN patients. This study focused on researching the differently expressed pattern of the...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26958
更新日期:2019-02-01 00:00:00
abstract::Cellular growth has been found to be directly related to the amount of sodium pumping activity in mouse lymphoblasts (L5178-Y) cultured in varying concentrations of the cardiac glycoside, ouabain. No short-term adaptation (within one generation) occured; i.e., neither growth rate nor (Na+ + K+)-ATPase activity increas...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040870313
更新日期:1976-03-01 00:00:00
abstract::Parathyroid hormone-related protein (PTHrP) (107-139), in contrast to the N-terminal fragment PTHrP (1-36), has been shown to interact with the vascular endothelial growth factor (VEGF) system to modulate human osteoblast differentiation. In this study, we evaluated whether this interaction might affect human osteobla...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21547
更新日期:2008-12-01 00:00:00