Abstract：
:Cellular growth has been found to be directly related to the amount of sodium pumping activity in mouse lymphoblasts (L5178-Y) cultured in varying concentrations of the cardiac glycoside, ouabain. No short-term adaptation (within one generation) occured; i.e., neither growth rate nor (Na+ + K+)-ATPase activity increased in cells cultured for 1-2 days in ouabain. Growth inhibition commenced after two hours, occurring concomitantly with decreased 3H-leucine incorporation into protein. The time course of this inhibition of protein synthesis, measured by leucine incorporation was similar to, but slightly slower than the time course or the dissipation of the sodium gradient. On the other hand, 3H-thymidine incorporation is unaffected by ouabain treatment over the same period. The uptake of 3H-alanine, a neutral amino acid thought to be transported via a Na+-dependent carri-r, was depressed concurrently with the sodium gradient dissipation. It is suggested, therefore, that ouabain inhibition of cellular growth results primarily from the dissipation of the sodium gradient leading to decreased Na+-dependent transport of amino acids (e.g., alanine) and, therefore, decreased protein synthesis, as observed by leucine incorporation. A sensitive and rapid method for determining ouabain inhibition of cell volume regulation is also described, which may prove potentially useful for assaying Na pump activity.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Shank BB,Smith NEdoi
10.1002/jcp.1040870313keywords:
subject
Has Abstractpub_date
1976-03-01 00:00:00pages
377-87issue
3eissn
0021-9541issn
1097-4652journal_volume
87pub_type
杂志文章abstract：:Chronic myeloid leukemia (CML) is a hematopoietic stem cell disorder caused by the oncogenic activity of the Bcr-Abl protein, a deregulated tyrosine kinase. Calcium may act directly on cellular enzymes and in conjunction with other cellular metabolites, such as cyclic nucleotides, to regulate cell functions. Alteratio...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22140
更新日期：2010-08-01 00:00:00
abstract：:The experiments reported were motivated by the observation that in vivo gangliosides promoted angiogenesis when the dose of the angiogenic factor was too low to be effective (Ziche et al.: Laboratory Investigation 61:629-634, 1989). As an approach to understanding the mechanism of this modulatory effect, we analysed t...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041440319
更新日期：1990-09-01 00:00:00
abstract：:K+ channels of the alveolar epithelium control the driving force acting on the ionic and solvent flow through the cell membrane contributing to the maintenance of cell volume and the constitution of epithelial lining fluid. In the present work, we analyze the effect of the Cl- channel inhibitors: (4-[(2-butyl-6,7-dich...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28396
更新日期：2019-08-01 00:00:00
abstract：:Phosphoribosylpyrophosphate synthetase activity was determined in Friend virus-inducted erythroleukemic cells in culture, stimulated to differentiate in the presence of dimethylsulfoxide. The activity of phosphoribosylpyrophosphate synthetase did not decrease in cells which had acquired the specialized function of hem...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040880208
更新日期：1976-06-01 00:00:00
abstract：:The ratio of mouse to rat albumin secreted by mouse hepatoma x rat hepatoma hybrid cells is constant (of the order of 5.0) irrespective of the total amounts produced. The present results establish for seven independent hybrid clones that the coordination in the ratio of mouse to rat product applies also at the level o...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041150211
更新日期：1983-05-01 00:00:00
abstract：:Selective spermatozoa movement from storage of the oviduct to fertilization site is suggested to be a result of chemotaxis. In the present study, Natriuretic peptide precursor A (NPPA) induced sperm chemotaxis in capillaries and enhanced intracellular Ca(2+) level, both of which could be blocked by the Natriuretic Pep...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22962
更新日期：2012-05-01 00:00:00
abstract：:Cancer incidences are growing and cause millions of deaths worldwide. Cancer therapy is one of the most important challenges in medicine. Improving therapeutic outcomes from cancer therapy is necessary for increasing patients' survival and quality of life. Adjuvant therapy using various types of antibodies or immunomo...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.28122
更新日期：2019-08-01 00:00:00
abstract：:Infection with Verotoxin producing Escherichia coli (VTEC) has been implicated in hemolytic uremic syndrome, the leading cause of pediatric renal failure. Verotoxin (VT) binds to globotriaosylceramide (Gal alpha 1-4Gal beta 1-4GlcCer Gb3) in susceptible cells. Gb3 is required for cytotoxicity and toxin-resistant cells...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041500324
更新日期：1992-03-01 00:00:00
abstract：:Previous studies suggested that the decreased collagen production observed in Kirsten sarcoma virus (Ki-MSV)-transformed BALB 3T3 cells could be reversed by treating cells with Bt2cAMP. We examined the relationship between intracellular cAMP, collagen production, and other properties in NRK and BALB 3T3 cells transfor...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041220117
更新日期：1985-01-01 00:00:00
abstract：:The mammalian olfactory system is a physiologically plastic region of the brain with the potential to support implanted stem cells. We performed direct injection of lineage-negative (lin-neg), green fluorescent protein-positive (GFP+) bone marrow cells into the olfactory bulb to assess cell survival and motility withi...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20123
更新日期：2005-01-01 00:00:00
abstract：:Tobacco smoking is associated with many diseases. Addiction is of the most notorious tobacco-related syndrome and is mainly attributed to nicotine. In this study, we employed Caenorhabditis elegans as a biological model to systemically investigate the effect of chronic nicotine exposure on microRNA (miRNA) expression ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24419
更新日期：2014-01-01 00:00:00
abstract：:The basis of the well-known decline in cell proliferation with increasing passage number of human diploid fibroblast-like cell cultures is not known. It has been found that DNA synthesis was deficient in the remaining but still proliferating cells, but when appropriate corrections reflecting the remaining dividing cel...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041430107
更新日期：1990-04-01 00:00:00
abstract：:The receptor activator of nuclear factor-kappaB ligand (RANKL) plays a critical role in the differentiation and bone resorptive activity of osteoclasts. Recently, the development of anti-resorptive agents from natural substances has become a subject of interest. Therefore, we evaluated the effects of 222 natural compo...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21892
更新日期：2009-12-01 00:00:00
abstract：:Cardiac fibroblast (CF) differentiation to myofibroblasts expressing α-smooth muscle actin (α-SMA) plays a key role in cardiac fibrosis. Therefore, a study of the mechanism regulating α-SMA expression is a means to understanding the mechanism of fibroblast differentiation and cardiac fibrosis. Previous studies have sh...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27471
更新日期：2019-05-01 00:00:00
abstract：:Cells isolated from fetal rat calvaria (RC) and maintained in vitro in medium containing ascorbic acid and B-glycerophosphate form three-dimensional, mineralized nodules having the histological, immunohistological, and ultrastructural characteristics of woven bone. We have studied the effects of forskolin (FSK), a dit...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041420109
更新日期：1990-01-01 00:00:00
abstract：:Intramedullary stabilization is frequently used to treat long bone fractures. Since implant removal can become technically very challenging with the potential to cause further tissue damage, biodegradable materials are emerging as alternative options. Magnesium (Mg)-based biodegradable implants have a controllable deg...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28736
更新日期：2019-11-01 00:00:00
abstract：:Biochemical events elicited by interleukin 1 (IL-1) were studied in Swiss 3T3 fibroblasts. One hour after its addition, IL-1 stimulated synthesis of prostaglandin E2 (PGE2), which continued to accumulate for 4 days. IL-1 also stimulated cAMP accumulation. Indomethacin blocked cAMP accumulation in response to IL-1, sug...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041390106
更新日期：1989-04-01 00:00:00
abstract：:Lung squamous cell carcinoma (LSCC) is a common cancer worldwide, and this study aimed to investigate the key regulatory networks and prognostic indicators of LSCC. MicroRNA (miRNA)/messenger RNA (mRNA) sequencing and DNA methylation data were obtained from the Cancer Genome Atlas. Differentially expressed miRNAs (DEm...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28852
更新日期：2019-12-01 00:00:00
abstract：:We investigated the role of mammalian target of rapamycin/nuclear factor-kappa B (mTOR/NF-κB) signaling pathway in high thoracic epidural anesthesia (HTEA) against myocardial ischemia-reperfusion (I/R) injury in rats. The rat model of myocardial I/R injury was established. Ninety rats were divided into the normal, sha...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26320
更新日期：2018-09-01 00:00:00
abstract：:The simultaneous exposure of WI-38 human fibroblasts (HF) to a heat shock (45 degrees C, 30 min) and an alkaline shift (> or = pH 8.0) in the incubation medium increased and extended the expression of heat shock proteins (hsps). Hsp70 was the most prominent inducible hsp synthesized during the recovery phase after the...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041620304
更新日期：1995-03-01 00:00:00
abstract：:C1q/tumor necrosis factor-related protein-6 (CTRP6) is a newly identified adipokine involved in diverse biological processes. However, its role in salivary glands remains unknown. Here, we demonstrated that CTRP6 was mainly distributed in the nuclei, apicolateral membranes, and cytoplasm of human submandibular glands ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.30262
更新日期：2021-01-05 00:00:00
abstract：:Cytoskeleton not only controls cell morphology but also regulates cell growth, migration, differentiation, and gene expression, events which are fundamental to embryogenesis, carcinogenesis, and wound healing. We have recently reported that reorganization of cytoskeleton induces expression of mRNA for transforming gro...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199708)172:2<192::AID-JCP6
更新日期：1997-08-01 00:00:00
abstract：:The actin- and myosin-binding protein, caldesmon (CaD) is an essential component of the cytoskeleton in smooth muscle and non-muscle cells and is involved in the regulation of cell contractility, division, and assembly of actin filaments. CaD is abundantly present in endothelial cells (EC); however, the contribution o...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20244
更新日期：2005-06-01 00:00:00
abstract：:We examined the behavior of the transcription factor Max during retrograde neuronal degeneration of retinal ganglion cells. Using immunohistochemistry, we found a progressive redistribution of full-length Max from the nucleus to the cytoplasm and dendrites of the ganglion cells following axon damage. Then, the axotomi...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10404
更新日期：2004-02-01 00:00:00
abstract：:Vero (African green monkey kidney) cells grown in tissue culture monolayer were sensitive to Clostridium perfringens enterotoxin. Within 30 minutes of exposure to the enterotoxin gross morphological damage was observed and within 40 minutes approximately 75% of the cells had detached. Nearly half of the cells were non...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040990205
更新日期：1979-05-01 00:00:00
abstract：:Derivation of insulin producing cells (IPCs) from embryonic stem (ES) cells provides a potentially innovative form of treatment for type 1 diabetes. Here, we discuss the current state of the art, unique challenges, and future directions on generating IPCs. ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.21615
更新日期：2009-02-01 00:00:00
abstract：:Cystamine together with colchicine markedly enhanced the uptake of [3H]-thymidine into DNA of quiescent cultures of insulin-stimulated Swiss 3T3 mouse fibroblasts. Flow cytofluorometric analyses showed an increased rate of transition of cells from G0/G1----S + G2 in response to combinations of insulin, colchicine, and...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041200307
更新日期：1984-09-01 00:00:00
abstract：:Sirtuin 3 (SIRT3) a mitochondrial enzyme that plays an important role in energy homeostasis, cardiac remodeling, and heart failure (HF). The expression of SIRT3 declines with advanced age, cardiovascular, and metabolic diseases. Accumulating evidence suggests that SIRT3 plays a critical role in protecting the heart fr...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.27200
更新日期：2019-03-01 00:00:00
abstract：:The herbicide 2,4,5 trichlorophenoxy acetic acid (245T) at concentrations from 0.5 to 0.9 mM, was found to inhibit respiration and then growth in exponentially growing cultures of Tetrahymena pyriformis. Cell division was stopped for periods up to 60 minutes after which the cells recovered and division resumed. Recove...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040850219
更新日期：1975-04-01 00:00:00
abstract：:The development of spike potential mechanisms during cell differentiation was studied in chick myotubes formed in vitro from trypsin-dissociated myoblasts. The spike potential and its rate of rise were measured in myotubes from 4-14 day old cultures. A depolarizing current pulse was delivered to evoke the spike potent...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040900307
更新日期：1977-03-01 00:00:00
