Abstract:
:Cells isolated from fetal rat calvaria (RC) and maintained in vitro in medium containing ascorbic acid and B-glycerophosphate form three-dimensional, mineralized nodules having the histological, immunohistological, and ultrastructural characteristics of woven bone. We have studied the effects of forskolin (FSK), a diterpene that activates adenylate cyclase, in this system. While 10(-7)-10(-5) M FSK significantly stimulated cAMP levels in RC cells, lower concentrations did not. cAMP levels with 10(-5) M FSK reached a maximum by 30 min at 37 degrees C and returned to basal level in 2-3 hr. Changes in cAMP levels correlated with changes in cellular shape: cells treated with 10(-5) M FSK assumed a stellate morphology, lost microfilament bundles, and reduced their substrate adhesiveness, while cells treated with 10(-9) M were not affected. Exponential growth and saturation densities of FSK-treated cultures were similar to untreated cultures, indicating that FSK was neither toxic nor stimulatory to the population. The effect on bone nodule formation of FSK present continuously depended on concentration: 10(-5) M FSK significantly inhibited the number of nodules formed, while 10(-9) M FSK significantly stimulated bone nodule formation. Single short treatments with either 10(-5) M or 10(-9) M FSK had no effect on nodule formation, but repeated short duration treatments (1 hr every 2 days for 21 days) gave results similar to continuous exposure. These results indicate that intermittent elevations in intracellular cAMP have an inhibitory effect on bone formation. In addition, our work indicates that low concentrations of FSK stimulate differentiation of osteoprogenitor cells possibly through a non-cAMP-dependent process.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Turksen K,Grigoriadis AE,Heersche JN,Aubin JEdoi
10.1002/jcp.1041420109subject
Has Abstractpub_date
1990-01-01 00:00:00pages
61-9issue
1eissn
0021-9541issn
1097-4652journal_volume
142pub_type
杂志文章abstract::Syndecan-1 belongs to a family of transmembrane proteoglycans, acts as a coreceptor for growth factor binding, as well as cell-matrix and cell-cell interactions, and is induced in smooth muscle cells (SMCs) following balloon catheter injury. In this report, we investigated syndecan-1 expression in SMCs in response to ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20927
更新日期:2007-04-01 00:00:00
abstract::Osteopontin (OPN) is a major non-collagenous bone matrix protein implicated in the regulation of cell function. Although OPN is rich in the cementum of the tooth, the significance of OPN in this tissue is not understood. Tooth root resorption is the most frequent complication of orthodontic tooth movement (TM). The ob...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21250
更新日期:2008-03-01 00:00:00
abstract::Cervical cancer is a deadly gynecological malignancy in need of innovative treatment strategies. Emerging preclinical data has suggested the benefits of nanocarriers over the traditional chemotherapy for cancer treatment. In particular, gold nanoparticles are gaining popularity due to gold's inert nature, limited side...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28316
更新日期:2019-02-20 00:00:00
abstract::The myelin basic protein (MBP) gene contains sequences located upstream of its transcription start site which play a key role in glial-specific transcription of the MBP promoter. Earlier analysis of the 320 bp upstream regulatory sequence of MBP has revealed multiple cis-acting regulatory motifs which differentially r...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041630213
更新日期:1995-05-01 00:00:00
abstract::The role of calmodulin (CaM) in modulating calcium (Ca) uptake by sarcoplasmic reticulum (SR) of vascular smooth muscle was studied in saponin skinned strips of rat caudal artery. Exogenous CaM concentrations ranging from 0.3-1.8 microM did not statistically change the steady state MgATP-dependent Ca content, the MgAT...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041530121
更新日期:1992-10-01 00:00:00
abstract::Endoplasmic reticulum (ER) stress, a common cellular stress response, is closely related to the activation of autophagy that is an important and evolutionarily conserved mechanism for maintaining cellular homeostasis. Autophagy induced by ER stress mainly includes the ER stress-mediated autophagy and ER-phagy. The ER ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.26137
更新日期:2018-05-01 00:00:00
abstract::Characteristic features like self-renewal, multilineage differentiation potential, and immune-modulatory/anti-inflammatory properties, besides the ability to mobilize and home distant tissues make stem cells (SCs) a lifeline for an individual. Stem cells (SCs) if could be harvested and expanded without any abnormal ch...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.27846
更新日期:2019-06-01 00:00:00
abstract::Exosomes extracted from mesenchymal stem cells (MSCs) was reported to reduce myocardial ischemia/reperfusion damage. Besides, stromal-derived factor 1 (SDF1a) functions as cardiac repair after myocardial infarction (MI). Therefore, the present study aims to identify whether exosomes (Exo) released from SDF1-overexpres...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28070
更新日期:2019-08-01 00:00:00
abstract::The expression profile and functions of the brain-enriched Rab22B/Rab31 small GTPase had remained uncharacterized. Using specific antibodies against Rab22B, we found the protein to be exceptionally enriched in nestin and RC2-positive radial glia of the embryonic mouse brain. In the adult brain, Rab22B is rather specif...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21911
更新日期:2009-12-01 00:00:00
abstract::Recently, ATP has gained much interest as an extracellular messenger involved in the communication of calcium signals between cells. The mechanism of ATP release is, however, still a matter of debate. In the present study we investigated the possible contribution of connexin hemichannels or ion channels in the release...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10365
更新日期:2003-11-01 00:00:00
abstract::High temperature requirement A1 (HTRA1) is a serine protease playing a modulatory role in various cell processes, particularly in the regulation of transforming growth factor-β (TGF-β) signaling. A deleterious role in late-onset cerebral small vessel diseases (CSVDs) of heterozygous HTRA1 mutations, otherwise causativ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29609
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND:Acute kidney injury (AKI) shows several kinds of disorders, which acutely harm the kidney. However, the current medical methods have limited therapeutic effects. The present study aimed to find out the molecular mechanism of AKI pathogenesis, which may provide new insights for future therapy. METHODS:Bioinf...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28118
更新日期:2019-08-01 00:00:00
abstract::Protease nexin-I (PN-I, Mr approximately 43,000) is representative of a newly described class of cell-secreted protease inhibitors. PN-I has been purified to apparent homogeneity, partially sequenced, and monospecific antibodies have been raised against it. PN-I is a potent inhibitor of urokinase, thrombin, plasmin, a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041320217
更新日期:1987-08-01 00:00:00
abstract::Phenotypically different osteoclasts may be generated from different subsets of precursors. To what extent the formation of these osteoclasts is influenced or mediated by the inflammatory cytokine TNF-α, is unknown and was investigated in this study. The osteoclast precursors early blasts (CD31hi Ly-6C- ), myeloid bla...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26024
更新日期:2017-12-01 00:00:00
abstract::The zafirlukast has been reported to be anti-inflammatory and widely used to alleviate the symptoms of asthma. However, its influence on insulin secretion in pancreatic β-cells has not been investigated. Herein, we examined the effects of zafirlukast on insulin secretion and the potential underlying mechanisms. Among ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26750
更新日期:2018-11-01 00:00:00
abstract::Expression of plasminogen activator inhibitor type-1 (PAI-1), a member of the SERPIN gene family that functions to regulate the plasmin-based pericellular proteolytic cascade, is growth state-regulated in normal rat kidney (NRK) cells (Ryan and Higgins, 1990, J. Cell. Physiol., 155:376-384; Ryan et al., 1996, Biochem....
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199701)170:1<8::AID-JCP2>3
更新日期:1997-01-01 00:00:00
abstract::Glucose and tumor necrosis factor-alpha (TNFalpha) concentrations are elevated in diabetes. Both of these factors correlate with diabetic vasculopathy and endothelial cell apoptosis, yet their combined effects have not been measured. We have previously shown that the angiogenic growth factor fibroblast growth factor-2...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21476
更新日期:2008-10-01 00:00:00
abstract::The binding of [3H]tuftsin to normal and in vivo stimulated mouse peritoneal macrophage populations was studied at 22 degrees C. The [3H]tuftsin binding to thioglycollate-stimulated macrophages was shown to be rapid and saturable, with an equilibrium dissociation constant (K(D)) (calculated from a Scatchard plot) of 5...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041000106
更新日期:1979-07-01 00:00:00
abstract::Previous studies from our laboratory have shown that Chinese hamster V79 cells mutated to high level resistance to amphotericin B have a lower cellular level of cholesterol, the target molecule for the polyene antibiotic. Two amphotericin B-resistant (AMBR) mutants were each hybridized to their parental amphotericin B...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041060106
更新日期:1981-01-01 00:00:00
abstract::Selenium (Se) deficiency induces typical clinical and pathological changes and causes various pathological responses at the molecular level in several different chicken organs; the kidney is one of the target organs of Se deficiency. To explore the mechanisms that underlie the effects of microRNA-33-3p (miR-33-3p) on ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28050
更新日期:2019-08-01 00:00:00
abstract::Mesenchymal stromal cells (MSCs) have been widely exploited for the treatment of several conditions due to their intrinsic regenerative and immunomodulatory properties. MSC have demonstrated to be particularly relevant for the treatment of ischemic diseases, where MSC-based therapies can stimulate angiogenesis and ind...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29622
更新日期:2020-10-01 00:00:00
abstract::Autophagy is a crucial cellular homeostatic process and an important part of the host defense system. Dysfunction in autophagy enhances tissue susceptibility to infection and multiple diseases. However, the role of nucleotide oligomerization domain 1 (NOD1) in autophagy in bovine hepatocytes is not well known. Therefo...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30227
更新日期:2020-12-24 00:00:00
abstract::When calcium is removed from culture medium, motility of cultured cells is decreased. The effect is rapid, reversible and pronounced. Decreased motility is observed with normal mouse Balb/c 3T3 cells, mouse L929 cells, rat kidney fibroblasts and chick embryo fibroblasts. The calcium dependence of movement can be obser...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041010112
更新日期:1979-10-01 00:00:00
abstract::Isolated hamster intestinal epithelial cells can be separated by velocity sedimentationion on 2-10% Ficoll gradients into three subpopulations of cells which differ in morphology, biochemistry, physiology, and membrane components. These subpopulations are not pure but are enriched in a single cell type to the extent t...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041110111
更新日期:1982-04-01 00:00:00
abstract::Heparin and related glycosaminoglycans are potent inhibitors of both in vivo and in vitro smooth muscle cell (SMC) proliferation. We have found that epidermal growth factor (EGF) reverses the antiproliferative effects of heparin. Other known SMC mitogens, including platelet-derived growth factor (PDGF), insulin-like g...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041310203
更新日期:1987-05-01 00:00:00
abstract::Serum-free mouse embryo cells, cultured in basal nutrient medium supplemented with insulin, transferrin, epidermal growth factor, fibronectin, and high-density lipoprotein, do not exhibit growth crisis, lack detectable chromosomal aberrations, are nontumorigenic in vivo, are dependent on epidermal growth factor for su...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041440110
更新日期:1990-07-01 00:00:00
abstract::The permeability of human platelets to glycerol was determined at 37 degrees C, 25 degrees C, and 0 degrees C from the rate of change of cell volume after abrupt addition of 0.5 mol/liter glycerol in phosphate-buffered saline. Intracellular water volume was measured employing both tritiated water and a photometric met...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041280118
更新日期:1986-07-01 00:00:00
abstract::Maintenance of intestinal epithelial barrier functions is crucial to prevent systemic contamination by microbes that penetrate from the gut lumen. GTPases of the Rho-family such as RhoA, Rac1, and Cdc42 are known to be critically involved in the regulation of intestinal epithelial barrier functions. However, it is sti...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22446
更新日期:2011-05-01 00:00:00
abstract::Excess reactive oxygen species (ROS) generated in embryos during in vitro culture damage cellular macromolecules and embryo development. Glutathione (GSH) scavenges ROS and optimizes the culture system. However, how exogenous GSH influences intracellular GSH and improves the embryo developmental rate is poorly underst...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27497
更新日期:2019-05-01 00:00:00
abstract::CD8+ T cells play a vital role in cancer immunotherapy and can be shaped by metabolism. Avasimibe is an acyl coenzyme A-cholesterol acyltransferase (ACAT) inhibitor, which has been clinically verified safe in other phase Ⅲ clinical trials. It can potentiate the killing function of CD8+ T cells by modulating cholestero...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29358
更新日期:2020-05-01 00:00:00