Abstract:
:Serum-free mouse embryo cells, cultured in basal nutrient medium supplemented with insulin, transferrin, epidermal growth factor, fibronectin, and high-density lipoprotein, do not exhibit growth crisis, lack detectable chromosomal aberrations, are nontumorigenic in vivo, are dependent on epidermal growth factor for survival, and are growth inhibited by serum or platelet-free plasma. These cells after transfection with the human Ha-ras or rat neu oncogenes no longer required epidermal growth factor for survival, were tumorigenic in vivo, and also proliferated in serum-containing medium. Autocrine activity capable of replacing epidermal growth factor was detected in conditioned medium from ras-transformed cultures, but little such activity was detected in medium from neu-transformed cultures. In addition, the capability of ras or neu-transformed cells to grow in serum-containing medium could not be mimicked in untransformed cells by the addition of growth factors or conditioned medium from transformed cells. These results suggest that the known structural similarity of the neu gene product to the EGF receptor is also reflected in a functional similarity by which the mutationally activated neu protein can replace the ligand-activated EGF receptor. These results also suggest that the ability of ras- and neu-transformed cells to escape the effect of the inhibitory serum activity is a nonautocrine property distinct from the acquisition of EGF autonomy.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Shirahata S,Rawson C,Loo D,Chang YJ,Barnes Ddoi
10.1002/jcp.1041440110subject
Has Abstractpub_date
1990-07-01 00:00:00pages
69-76issue
1eissn
0021-9541issn
1097-4652journal_volume
144pub_type
杂志文章abstract::Glucose and tumor necrosis factor-alpha (TNFalpha) concentrations are elevated in diabetes. Both of these factors correlate with diabetic vasculopathy and endothelial cell apoptosis, yet their combined effects have not been measured. We have previously shown that the angiogenic growth factor fibroblast growth factor-2...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21476
更新日期:2008-10-01 00:00:00
abstract::The myelin basic protein (MBP) gene contains sequences located upstream of its transcription start site which play a key role in glial-specific transcription of the MBP promoter. Earlier analysis of the 320 bp upstream regulatory sequence of MBP has revealed multiple cis-acting regulatory motifs which differentially r...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041630213
更新日期:1995-05-01 00:00:00
abstract::Estrogens mediate gene transcription and signaling of numerous cellular processes in a variety of tissues, including the bone, breast, and endometrium, through binding and activation of estrogen receptors (ERs). Estrogen-mediated ER agonist activity has shown benefit in conditions related to estrogen deficiency in wom...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.24324
更新日期:2013-07-01 00:00:00
abstract::The level of poly A+ RNA in growing cultures of human diploid fibroblasts is 1.8-fold times greater than in resting cultures. The level of functional ribosomes in growing cultures is 2.8 times that in resting cultures. Since transit times are similar in both types of cells, it can be concluded that the rate of protein...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040940212
更新日期:1978-02-01 00:00:00
abstract::Ascorbic acid induces apoptosis, autophagy, and necrotic cell death in cancer cells. We investigated the mechanisms by which ascorbic acid induces death in laryngeal squamous cell carcinoma Hep2 cells. Ascorbic acid markedly reduced cell viability and induced death without caspase activation and an increase in cytochr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25438
更新日期:2017-02-01 00:00:00
abstract::Anoctamin1 (ANO1) encodes a Ca(2+)-activated chloride (Cl(-)) channel (CaCC) in variety tissues of many species. Whether ANO1 expresses and functions as a CaCC in cardiomyocytes remain unknown. The objective of this study is to characterize the molecular and functional expression of ANO1 in cardiac myocytes and the ro...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
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更新日期:2015-02-01 00:00:00
abstract::In this study, we developed an injectable in situ forming hydrogel/microparticle system consisting of two drugs, melatonin and methylprednisolone, to investigate the capability of the system for chondrogenesis in vitro and in vivo. The chemical, mechanical, and rheological properties of the hydrogel/microparticle were...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30006
更新日期:2021-03-01 00:00:00
abstract::NGF appears to be involved in spermatogenesis. However, mice lacking NGF or TrkA genes do not survive more than a few days whereas p75(NTR) knockout mice are viable and fertile. Therefore, we addressed the effect of betaNGF on spermatogenesis by using the systems of rat germ cell culture we established previously. bet...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20805
更新日期:2007-01-01 00:00:00
abstract::Electrical stimulation (ES) has been shown to improve some of impairments after spinal cord injury (SCI), but the underlying mechanisms remain unclear. The Wnt signaling pathways and the endocannabinoid system appear to be modulated in response to SCI. This study aimed to investigate the effect of ES therapy on the ac...
journal_title:Journal of cellular physiology
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更新日期:2020-12-01 00:00:00
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journal_title:Journal of cellular physiology
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doi:10.1002/jcp.24433
更新日期:2014-02-01 00:00:00
abstract::In spite of the apparent depletion of mast cells in tissues of mutant mice of W/Wv genotype, cells with many features of mast cells do develop when bone marrow cells of W/Wv mice are cultured in the presence of pokeweed mitogen-stimulated spleen cell-conditioned medium (PWM-SCM). In order to resolve this discrepancy a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041340109
更新日期:1988-01-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a fatal and disabling neurodegenerative disease characterized by upper and lower motor neurons depletion. In our previous work, comprehensive genomic profiling of 41 motor cortex samples enabled to discriminate controls from sporadic ALS patients, and segregated these latter into...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26182
更新日期:2018-04-01 00:00:00
abstract::Over the past decade, a wide range of scientific investigations have been performed to reveal neuropathological aspects of cognitive disorders; however, only limited therapeutic approaches currently exist. The failures of conventional therapeutic options as well as the predicted dramatic rise in the prevalence of cogn...
journal_title:Journal of cellular physiology
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doi:10.1002/jcp.27289
更新日期:2019-03-01 00:00:00
abstract::Integrins are heterodimeric transmembrane proteins that play important roles in various biological processes. Most integrins serve as adhesion molecules and transmit bidirectional signaling across the cell membrane through global conformational changes from the bent closed to the extended open conformation. However, i...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.30242
更新日期:2020-12-27 00:00:00
abstract::To evaluate the growth-inhibitory properties of the potent multi-kinase antagonist Regorafenib (Fluoro-Sorafenib), which was synthesized as a more potent Sorafenib, a Raf inhibitor and to determine whether similar mechanisms were involved, human hepatoma cell lines were grown in the presence or absence of Regorafanib ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24148
更新日期:2013-02-01 00:00:00
abstract::A sialoglycopeptide (SGP), isolated and purified from bovine cerebral cortex cells, was studied in regard to early signal transduction events associated with the cell cycle. Previously shown to be a potent antagonist to a variety of mitogens, the SGP abrogated the ability of 12-O-tetradecanoylphorbol-13 acetate (TPA) ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041470214
更新日期:1991-05-01 00:00:00
abstract::There are two known phosphorylation-mediated inactivation mechanisms for TRPC3 channels. Protein kinase G (PKG) inactivates TRPC3 by direct phosphorylation on Thr-11 and Ser-263 of the TRPC3 proteins, and protein kinase C (PKC) inactivates TRPC3 by phosphorylation on Ser-712. In the present study, we explored the rela...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20567
更新日期:2006-05-01 00:00:00
abstract::Adhesion of calf lens epithelial cells to lens capsule, their natural basement membrane was found to be considerably more rapid than either to plastic or to type I or type IV collagen coated surfaces. No polarity of the basement membrane was observed as the cells were able to attach to either side of the anterior or p...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041130215
更新日期:1982-11-01 00:00:00
abstract::Transforming growth factor beta type 1 (TGF-beta 1) was reacted with NHS-biotin to yield a derivative of TGF-beta 1 which was biotinylated on lysine residues. The biotinylated form of TGF-beta 1 was separated from the unreacted material by reverse phase chromatography. In three separate bioassays, the derivatized pept...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041410125
更新日期:1989-10-01 00:00:00
abstract::We previously revealed that epithelial-to-mesenchymal transition (EMT) was mediated by ΔNp63β, a splicing variant of ΔNp63, in oral squamous cell carcinoma (OSCC). Recent studies have highlighted the involvement of microRNA (miRNA) in EMT of cancer cells, though the mechanism remains unclear. To identify miRNAs respon...
journal_title:Journal of cellular physiology
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更新日期:2018-10-01 00:00:00
abstract::The F-40 cell line, a stable variant of LM fibroblasts selected for its resistance to polyethylene glycol (PEG)-induced fusion (Roos and Davidson: Somatic Cell and Molecular Genetics 6:381-391, 1980), has a decreased capacity to internalize fluid-phase markers and nonspecifically surface-bound macromolecules. It is no...
journal_title:Journal of cellular physiology
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更新日期:1988-12-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:1996-05-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00
abstract::Diabetic nephropathy (DN) is a kind of microvascular complications of diabetes. Long noncoding RNAs (lnRNAs) can participate in the development of various diseases, including DN. However, the function of lncRNA NEAT1 is unclear. In our present study, we reported that NEAT1 was significantly increased in streptozotocin...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
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更新日期:2019-08-01 00:00:00
abstract::Interleukin (IL)-18 is a pro-inflammatory cytokine belonging to the IL-1 family, first identified for its interferon-γ-inducing properties. IL-18 regulates both T helper (Th) 1 and Th2 responses. It acts synergistically with IL-12 in the Th1 paradigm, whereas with IL-2 and without IL-12 it can induce Th2 cytokine prod...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.30008
更新日期:2021-03-01 00:00:00
abstract::Osteoporosis, arthritis, Peget's disease, bone tumor, periprosthetic joint infection, and periprosthetic loosening have a common characteristic of osteolysis, which is characterized by the enhanced osteoclastic bone resorptive function. At present, the treatment target of these diseases is to interfere with osteoclast...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27888
更新日期:2019-07-01 00:00:00
abstract::The stromal cell-derived factor-1 (SDF-1)/CXC receptor 4 (CXCR4) axis has been shown to play a role in colorectal cancer progression. In addition, the protease urokinase-type plasminogen activator (uPA) is an important factor in tumor cell invasion and metastasis. However, the mechanism by which SDF-1 mediates uPA exp...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22831
更新日期:2012-03-01 00:00:00
abstract::Human fibroblasts derived from newborn foreskin and designated CF-3 were assayed for heat labile glucose-6-phosphate dehydrogenase (G6PD) when they had grown to confluency, as well as when they were arrested in an essentially nonmitotic state. Under both culture conditions there was an increase in heat labile G6PD (up...
journal_title:Journal of cellular physiology
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doi:10.1002/jcp.1040930108
更新日期:1977-10-01 00:00:00
abstract::Mouse L-929 cells were subjected to increasing concentrations of sorbitol, which remove cell water and reduce volume osmotically. The rate of lactate production from glucose was significantly higher in osmotically perturbed cells than in controls, both in monolayers and in suspensions. L cells can apparently use sorbi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041420223
更新日期:1990-02-01 00:00:00
abstract::The actions of prostaglandins (PG) on cAMP in dispersed chief cells from guinea pig stomach were examined and compared to the actions of these agents on pepsinogen secretion. Maximal concentrations of A, B, or E prostaglandins caused a 2-5-fold increase in pepsinogen secretion and cellular cAMP. The relative order of ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041320119
更新日期:1987-07-01 00:00:00