Abstract:
:The stromal cell-derived factor-1 (SDF-1)/CXC receptor 4 (CXCR4) axis has been shown to play a role in colorectal cancer progression. In addition, the protease urokinase-type plasminogen activator (uPA) is an important factor in tumor cell invasion and metastasis. However, the mechanism by which SDF-1 mediates uPA expression in human colorectal cancer cells remains unknown. We investigated the molecular mechanism governing the interaction between SDF-1 stimulation and uPA expression in three human colon cancer cell lines (DLD-1, SW48, and COLO 205). We found that SDF-1 stimulation led to an increase in the expression and secretion of uPA in these cells. Experiments involving specific inhibitors and small interfering RNA demonstrated that the activation of p38 mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/Akt pathways are critical for SDF-1-induced uPA expression. Analysis of transcription factor binding using ELISA and chromatin immunoprecipitation assays revealed that SDF-1 increased Sp1- and AP-1-DNA-binding activities in DLD-1 cells. Inhibition of Sp1 and AP-1 activation blocked the SDF-1-induced expression and activity of the uPA promoter. The effect of SDF-1 on DLD-1 signaling and uPA expression was mediated by the CXCR4/β1 integrin axis. In summary, our findings elucidate the mechanisms of SDF-1/CXCR4 downstream signaling and provide insights into the function of SDF-1 in colon cancer cells.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Huang WS,Chin CC,Chen CN,Kuo YH,Chen TC,Yu HR,Tung SY,Shen CH,Hsieh YY,Guo SE,Shi CS,Liu TJ,Kuo HCdoi
10.1002/jcp.22831subject
Has Abstractpub_date
2012-03-01 00:00:00pages
1114-22issue
3eissn
0021-9541issn
1097-4652journal_volume
227pub_type
杂志文章abstract::Oxygen tension plays an important role in the regulation of cellular processes. During hematopoietic stem cell (HSC) differentiation, HSCs migrate from one stem cell niche to the next, each with a different oxygen tension that determines which signaling pathways are on and off, determining the differentiation stage of...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24348
更新日期:2013-09-01 00:00:00
abstract::The continuous discovery of human mesenchymal stem cells (hMSCs) in different tissues is stirring up a tremendous interest as a cell source for regenerative medicine therapies. Historically, hMSCs have been always considered a sub-population of mononuclear cells present in the bone marrow (BM). Although BM-hMSCs are s...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.24223
更新日期:2013-04-01 00:00:00
abstract::Cardiac hypertrophy is the main cause of heart failure and sudden death in patients. But the pathogenesis is unclear. Angiotensin II may contribute to cardiac hypertrophy in response to pressure overload. In angiotensin II-treated cardiomyocytes, there is a larger cross-sectional area, more apoptosis cells, and a redu...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28382
更新日期:2019-08-01 00:00:00
abstract::The components of unidirectional K influx and efflux have been investigated in the 3T3 cell and the SV40 transformed 3T3 cell in expontntial and stationary growth phase. Over the cell densities used for transport experiments the 3T3 cell goes from exponential growth to density dependent inhibition of growth (4 X 10(4)...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040890306
更新日期:1976-11-01 00:00:00
abstract::The nuclear lamina is a fibrous structure at the nucleoplasmic surface of the inner nuclear membrane. Its assembly state is regulated by phosphorylation of its protein components, the lamins A, B, and C. The isoprenylation of the lamins is essential for their proper membrane anchoring and functionality. The content an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199705)171:2<135::AID-JCP3
更新日期:1997-05-01 00:00:00
abstract::A quantitative assay for intercellular adhesion, which is a modification of the collecting aggregate assay (Roth et al., '71) is described. The use of mechanically dissociated single cells labeled with 3H-leucine, and of a gyratory shaker increased considerably the efficiency of cell collection. With chick embryo neur...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040900104
更新日期:1977-01-01 00:00:00
abstract::Glucose and tumor necrosis factor-alpha (TNFalpha) concentrations are elevated in diabetes. Both of these factors correlate with diabetic vasculopathy and endothelial cell apoptosis, yet their combined effects have not been measured. We have previously shown that the angiogenic growth factor fibroblast growth factor-2...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21476
更新日期:2008-10-01 00:00:00
abstract::We compared the effects of 95% O2 (hyperoxia) alone, endotoxin (20 ng/ml) alone, and 95% O2 plus endotoxin on the release of lactate dehydrogenase (LDH), uptake of 5-hydroxytryptamine (5-HT), and antioxidant enzyme activities in porcine pulmonary arterial and aortic endothelial cells in monolayer culture. Hyperoxia in...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041220212
更新日期:1985-02-01 00:00:00
abstract::We describe the isolation and characterization of a Chinese hamster ovary cell line selected for resistance to N-carbamoyloxyurea. Using the mammalian cell permeabilization assay developed in our laboratory, a detailed analysis of the target enzyme, ribonucleotide reductase (EC 1.17.4.1), was carried out. Both drug-re...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041060218
更新日期:1981-02-01 00:00:00
abstract::Serine racemase (SR) is responsible for the biosynthesis of D-serine (D-Ser), an endogenous co-agonist for the glycine (Gly)-binding site on N-methyl-D-aspartate (NMDA) receptors, from L-Ser in the brain. We have previously demonstrated high expression of SR by chondrocytes in cartilage. In this study, we attempted to...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21821
更新日期:2009-09-01 00:00:00
abstract::Pancreatic ductal adenocarcinoma (PDAC) is one of the most frequently diagnosed cancers and the fourth leading cause of cancer-related death in the United States, suggesting that there is an urgent need to design novel strategies for achieving better treatment outcome of patients diagnosed with PDAC. Our previous stud...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,收录出版
doi:10.1002/jcp.24162
更新日期:2013-03-01 00:00:00
abstract::Colorectal cancer (CRC) is the third most common cancer and a leading cause of cancer-related death worldwide. About 1.8 million new cases were reported in 2018. Accordingly, there is an urgent need for development of effective strategies to lower the mortality and morbidity originated from this malignancy. MicroRNAs ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28872
更新日期:2019-12-01 00:00:00
abstract::BAG3 is a member of human BAG (Bcl-2-associated athanogene) proteins and plays a role in apoptosis, cell adhesion, cytoskeleton remodeling, and autophagy. The aim of this study was to evaluate BAG3 levels in healthy subjects, hypertensive patients, and hypertensive diabetic patients. We enrolled 209 Caucasian adults, ...
journal_title:Journal of cellular physiology
pub_type: 临床试验,杂志文章
doi:10.1002/jcp.26093
更新日期:2018-03-01 00:00:00
abstract::A factor, termed neutrophil alkaline phosphatase-inducing factor (NAP-IF), that has the capacity to increase the NAP activity of granulocytes was characterized by using two samples: cystic fluid (CF) and conditioned medium of a tumor cell line (T3M5). The molecular weight of NAP-IF was shown to be between 13,000 and 4...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041240213
更新日期:1985-08-01 00:00:00
abstract::A serum factor which enhances the growth/viability of SV40 transformed 3T3 mouse fibroblasts, but not untransformed 3T3 cells in tissue culture has been partially purified from calf serum. The purification, which involves acidification to pH 2, chromatography on Sephadex G-100 at pH 2 followed by either Sephadex G-25 ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040960203
更新日期:1978-08-01 00:00:00
abstract::Optical tweezers were used to scan individual Chronic Myelogenous Leukemia cells to determine if the cell death depends on the scanning conditions. Although increasing the scanning frequency or amplitude means greater force applied to the cells, their effects on cell death are not a simple increasing trend, as observe...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24369
更新日期:2013-10-01 00:00:00
abstract::The statins (including mevastatin and lovastatin) are a widely prescribed class of serum-cholesterol lowering drugs that function by inhibiting 3-hydroxymethylglutaryl coenzyme A (HMG CoA) reductase activity and cellular sterol synthesis. Statins are also widely being appreciated for their inhibitory effects upon infl...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20271
更新日期:2005-07-01 00:00:00
abstract::AMP-activated protein kinase (AMPK) has recently emerged as a potential target for cancer therapy due to the observation that activation of AMPK inhibits tumor cell growth. It is well-known that androgen receptor (AR) signaling is a major driver for the development and progression of prostate cancer and that downregul...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24494
更新日期:2014-06-01 00:00:00
abstract::Gene therapy using biocompatible cationic liposomes is amongst promising approaches that decreases death from cancers. Here an invasive multidrug resistant cell model has been developed by lentiviral transfection. In parallel phospholipids have been covalently conjugated to TAT, MMP2, and Herceptin. The functional lip...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28683
更新日期:2019-11-01 00:00:00
abstract::The actin- and myosin-binding protein, caldesmon (CaD) is an essential component of the cytoskeleton in smooth muscle and non-muscle cells and is involved in the regulation of cell contractility, division, and assembly of actin filaments. CaD is abundantly present in endothelial cells (EC); however, the contribution o...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20244
更新日期:2005-06-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a fatal and disabling neurodegenerative disease characterized by upper and lower motor neurons depletion. In our previous work, comprehensive genomic profiling of 41 motor cortex samples enabled to discriminate controls from sporadic ALS patients, and segregated these latter into...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26182
更新日期:2018-04-01 00:00:00
abstract::Adenosine is an ubiquitous nucleoside present in all body cells. It is released from metabolically active or stressed cells and subsequently acts as a regulatory molecule through binding to specific A1, A2A, A2B and A3 cell surface receptors. The synthesis of agonists and antagonists to the adenosine receptors and the...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/1097-4652(200101)186:1<19::AID-JCP1011>3.0
更新日期:2001-01-01 00:00:00
abstract::Peripheral nerve physiology and regeneration has been observed and investigated in literature but surgical applications to reconstruct and restore motor or sensory functions are still in a developmental phase. The peripheral nerve progresses slowly and incompletely compared with other tissues, it may provoke separatio...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.27299
更新日期:2019-04-01 00:00:00
abstract::Diabetes mellitus (DM) induces bone deterioration, while mechanical stimulation promotes osteocyte-driven bone formation. We aimed to evaluate the interaction of acute exposure (24 h) to high glucose (HG) with both the pro-survival effect conferred to osteocytic MLO-Y4 cells and osteoblastic MC3T3-E1 cells by mechanic...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25829
更新日期:2017-12-01 00:00:00
abstract::Topical intranasal application of the antifungal Amphotericin B (AmphoB) has been shown as an effective medical treatment of chronic rhinosinusitis. Because this antibiotic forms channels in lipid membranes, we considered the possibility that it affects the properties and/or cell surface expression of ion channels/pum...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20347
更新日期:2005-09-01 00:00:00
abstract::The effects of pulsatile and steady fluid flow on the mRNA levels of proto-oncogenes c-fos, c-jun, and c-myc in cultured human umbilical vein endothelial cells (HUVEC) were investigated. c-fos mRNA levels in stationary cultures were very low. A 1 Hz pulsatile flow with an average shear stress of 16 dynes/cm2 induced a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041540118
更新日期:1993-01-01 00:00:00
abstract::Several proteases and their specific inhibitors modulate the interdependent processes of cell migration and matrix proteolysis as part of the global program of trauma repair. Expression of plasminogen activator inhibitor type-1 (PAI-1), a serine protease inhibitor (SERPIN) important in the control of barrier proteolys...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20016
更新日期:2004-08-01 00:00:00
abstract::The significance of low pH-induced stimulation of osteoclastic bone resorption has recently been questioned following the finding that embryonic chick osteoclasts were only weakly stimulated by extremely low pH (6.5) and that the effect was transient, apparently due to cytotoxicity. Although low pH in the range 6.8-7....
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041540309
更新日期:1993-03-01 00:00:00
abstract::Anti-angiogenic agents regulate tumor growth by inhibiting endothelial cell proliferation and invasion. Carboxyamido-triazole (CAI), an inhibitor of non-voltage-operated calcium entry and calcium influx-mediated pathways, has angiogenesis and invasion inhibitory activity. We hypothesized that CAI may express its anti-...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10350
更新日期:2003-10-01 00:00:00
abstract::Syndecan-1 belongs to a family of transmembrane proteoglycans, acts as a coreceptor for growth factor binding, as well as cell-matrix and cell-cell interactions, and is induced in smooth muscle cells (SMCs) following balloon catheter injury. In this report, we investigated syndecan-1 expression in SMCs in response to ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20927
更新日期:2007-04-01 00:00:00