Abstract:
:Amyotrophic lateral sclerosis (ALS) is a fatal and disabling neurodegenerative disease characterized by upper and lower motor neurons depletion. In our previous work, comprehensive genomic profiling of 41 motor cortex samples enabled to discriminate controls from sporadic ALS patients, and segregated these latter into two distinct subgroups (SALS1 and SALS2), each associated with different deregulated genes. In the present study, we focused our attention on two of them, Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) and its type 1 receptor (PAC1R), and validated the results of the transcriptome experiments by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), immunohistochemistry and Western blot analysis. To assess the functional role of PACAP and PAC1R in ALS, we developed an in vitro model of human induced pluripotent stem cells (iPSC)-derived motor neurons and examined the trophic effects of exogenous PACAP following neurodegenerative stimuli. Treatment with 100 nm PACAP was able to effectively rescue iPSC-derived motor neurons from apoptosis, as shown by cell viability assay and protein dosage of the apoptotic marker (BAX). All together, these data suggest that perturbations in the PACAP-PAC1R pathway may be involved in ALS pathology and represent a potential drug target to enhance motor neuron viability.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Bonaventura G,Iemmolo R,D'Amico AG,La Cognata V,Costanzo E,Zappia M,D'Agata V,Conforti FL,Aronica E,Cavallaro Sdoi
10.1002/jcp.26182subject
Has Abstractpub_date
2018-04-01 00:00:00pages
3343-3351issue
4eissn
0021-9541issn
1097-4652journal_volume
233pub_type
杂志文章abstract::Multicolor immunostaining analysis is often a desirable tool in cell biology for most researchers. Nonetheless, this is not an easy task and often not affordable by many laboratories as it might require expensive instrumentation and sophisticated analysis software. Here, we describe a simple protocol for performing se...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24506
更新日期:2014-06-01 00:00:00
abstract:OBJECTIVE:Disc degeneration is the common life-threatening disease characterized by flank pain. The gene expression of insulin-like growth factor binding protein 3 (IGFBP3) is increased in patients with disc degeneration, however, its mechanism is still unknown. This study aimed to investigate the influence of IGFBP3 g...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27613
更新日期:2019-06-01 00:00:00
abstract::Radiotherapy remains a prime approach to adjuvant therapies in patients with early and advanced breast cancer. In spite of therapeutic success, metastatic progression in patients undergoing therapy, limits its application. However, effective therapeutic strategies to understand the cellular and molecular machinery in ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24780
更新日期:2015-03-01 00:00:00
abstract::The signaling pathways that stimulate glucose uptake in response to muscle contraction are not well defined. Recently, we showed that carbachol, an acetylcholine analog, stimulates contraction of C2C12 myotube cultures and the rapid arrival of myc-epitope tagged GLUT4 glucose transporters at the cell surface. Here, we...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22320
更新日期:2011-01-01 00:00:00
abstract::Vascular endothelial cells (EC), communicating with one another across gap junctions, are usually made dysfunctional by hypoxia and reoxygenation (H/R); however, very limited information exists regarding the effects of H/R on the endothelial gap junctions. We investigated whether H/R interferes with endothelial gap ju...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199909)180:3<305::AID-JCP1
更新日期:1999-09-01 00:00:00
abstract::Adiponectin, one of the adipose-derived hormone with metabolic activity, has been reported to conversely affect angiogenesis of endothelial cells in vitro. The previous study in animal models has demonstrated that adiponectin has a protective role in retinal vascular injury following pathological stimuli. However, cli...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28659
更新日期:2019-11-01 00:00:00
abstract::Wound healing is critically affected by age, ischemia, and growth factors such as TGFbeta1. The combined effect of these factors on fibroblast migration, an essential component of wound healing, is poorly understood. To address this deficiency, we examined expression of TGFbeta receptor type I and II (TGFbetaRI and RI...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10060
更新日期:2002-02-01 00:00:00
abstract::The biosynthesis and turnover of nicotinamide adenine dinucleotide (NAD) have been examined in mitotic cells of the human culture line, D98/AH2. No significant difference in the incorporation of nicotinic acid or nicotinamide could be detected between mitotic and interphase cells. The distribution of newly-incorporate...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040860509
更新日期:1975-12-01 00:00:00
abstract::Four temperature-sensitive (ts) mutants of rat 3Y1 fibroblasts, representing independent complementation groups, cease to proliferate predominantly with a 2n DNA content, at the restrictive temperature (39.8 degrees C) (temperature arrest) or at the permissive temperature (33.8 degrees C) at a confluent cell density (...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041230310
更新日期:1985-06-01 00:00:00
abstract::Mammalian sperm require to spend a limited period of time in the female reproductive tract to become competent to fertilize in a process called capacitation. It is well established that HCO3- is essential for capacitation because it activates the atypical soluble adenylate cyclase ADCY10 leading to cAMP production, an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25634
更新日期:2017-06-01 00:00:00
abstract::Butyrate and acetate are bacterial metabolites present in the large intestine lumen. Although butyrate is well known to inhibit the in vitro proliferation of human colon carcinoma cells in a process involving the hyperacetylation of specific nuclear histones, little is known about the possible link between butyrate me...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21556
更新日期:2009-01-01 00:00:00
abstract::Integrins are heterodimeric transmembrane proteins that play important roles in various biological processes. Most integrins serve as adhesion molecules and transmit bidirectional signaling across the cell membrane through global conformational changes from the bent closed to the extended open conformation. However, i...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.30242
更新日期:2020-12-27 00:00:00
abstract::The protease nexins (PN-I, Mr approximately 38,000; PN-II, Mr approximately 95,000; and PN-III, Mr approximately 31,000) are recently described cell-secreted proteins that selectively link to regulatory serine proteases in the extracellular environment and mediate their cellular binding, internalization, and degradati...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041170314
更新日期:1983-12-01 00:00:00
abstract::In this present study, Oreochromis mossambicus tilapia were transferred to cold water at 12°C for various time intervals (1, 4, 8, 24, and 48 hr) and its innate immune response was analyzed by studying cellular and humoral parameters. In vivo, alternative complement pathway activity in blood plasma was rapidly increas...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28270
更新日期:2019-02-11 00:00:00
abstract::The mitogenic effect of recombinant human erythropoietin (rHuEpo) on primary cultures of neonatal rat cardiac myocytes was observed. rHuEpo triggered a dose-dependent increase in myocyte proliferation. The hormone effect over optimally grown control culture 1 day after addition was maximum with 0.5 U/ml and was inhibi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199606)167:3<461::AID-JCP1
更新日期:1996-06-01 00:00:00
abstract::Cells are quite sensitive to a change of the extracellular pH and respond to it through detection of the H+/HCO3- level in extracellular fluid. However, little is known about molecular details induced by acidosis, such as intracellular pathways and gene expression. Here we describe properties of gene expression, prote...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20369
更新日期:2005-10-01 00:00:00
abstract::Human lymphocytes were equilibrated for 48 hours at 5-6 mM K+ ex over a range of temperatures between 0 and 37 degrees C, and at 5 degrees C over a range of external K+ levels between 0 and 32 mM. Cell K+ and Na+ contents are normal between 37 and 10 decrees. Below 10 degrees there is a critical thermal transition in ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041030113
更新日期:1980-04-01 00:00:00
abstract::The level of 1,25(OH)2D3 receptors in cultured mouse osteoblast-like (OB) cells is modulated by the rate of cell proliferation. We have studied two 1,25(OH)2D3-induced bioresponses to ascertain whether the changes in receptor levels during growth in culture alter cell responsiveness. Nuclear receptor levels were high ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041260104
更新日期:1986-01-01 00:00:00
abstract::Neuroblastoma (NB) is the leading pediatric cancer known for its heterogeneity and clinical aggressiveness leading to chemoresistance. Recent evidence in small RNA research has led to the discovery of PIWI-interacting RNAs (piRNAs) which work in an orchestrated fashion to modulate gene expression both in homeostatic c...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29136
更新日期:2020-03-01 00:00:00
abstract::Glial fibrillary acidic protein (GFAP) is an intermediate filament that is expressed in specifically expressed auricular chondrocytes, which are good cell sources of cartilage regenerative medicine. Although our group uses GFAP as a biomarker of matrix production in the cultured auricular chondrocytes, the biological ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25771
更新日期:2017-11-01 00:00:00
abstract::We describe the isolation and characterization of a Chinese hamster ovary cell line selected for resistance to N-carbamoyloxyurea. Using the mammalian cell permeabilization assay developed in our laboratory, a detailed analysis of the target enzyme, ribonucleotide reductase (EC 1.17.4.1), was carried out. Both drug-re...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041060218
更新日期:1981-02-01 00:00:00
abstract::We have previously demonstrated that kidney embryonic structures are present in rats, and are still developing until postnatal Day 20. Consequently, at postnatal Day 10, the rat renal papilla contains newly formed collecting duct (CD) cells and others in a more mature stage. Performing primary cultures, combined with ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28016
更新日期:2019-08-01 00:00:00
abstract::We determined whether the multi-kinase inhibitor sorafenib or its derivative regorafenib interacted with phosphodiesterase 5 (PDE5) inhibitors such as Viagra (sildenafil) to kill tumor cells. PDE5 and PDGFRα/β were over-expressed in liver tumors compared to normal liver tissue. In multiple cell types in vitro sorafeni...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24961
更新日期:2015-09-01 00:00:00
abstract::A monoclonal antibody (mAb), 2E12, against the neural cell adhesion molecule L1 recognized the 200 kDa component of L1. The epitope of L1 reacting with mAb 2E12 was localized in its carbohydrate chain, judging from the results of experiments on glycopeptidase F treatment. The physiological effect of adding mAbL1 (2E12...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041530211
更新日期:1992-11-01 00:00:00
abstract::Keratin intermediate filaments play an important role in maintaining the integrity of the skin structure. Understanding the importance of this subject is possible with the investigation of keratin defects in epidermolysis bullosa simplex (EBS). Nowadays, in addition to clinical criteria, new molecular diagnostic metho...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.26898
更新日期:2018-01-01 00:00:00
abstract::Data on the biological effects of some overexpressed oncogenes and their cooperation with cellular factors are, at least partially, contradictory. There are reports on the strong pro-apoptotic action of temperature-sensitive (ts) p53(135val) in transformed cells at permissive temperature. However, in our experience ve...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21705
更新日期:2009-05-01 00:00:00
abstract::Balb/C3T3 cells entered the quiescent Go state following serum deprivation. On addition of fresh serum, more than 95% of the culture resumed growth, but with asynchronous kinetics. If hydroxyurea were added just before the first cells reached S phase, at at least 90% of the cells accumulated at the Gl/S border over th...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040900219
更新日期:1977-02-01 00:00:00
abstract::Immunotherapy has caused a paradigm shift in the treatment of several malignancies, particularly the blockade of programmed death-1 (PD-1) and its specific receptor/ligand PD-L1 that have revolutionized the treatment of a variety of malignancies, but significant durable responses only occur in a small percentage of pa...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.29491
更新日期:2020-07-01 00:00:00
abstract::Platelet-derived growth factor (PDGF) is mitogenic and chemotactic for vascular smooth muscle cells cultured in vitro, and, thus, may play a role in the smooth muscle cell proliferation and migration that occurs during atherosclerotic lesion development. Two related PDGF polypeptides, designated as the A and B chains,...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041470116
更新日期:1991-04-01 00:00:00
abstract::Mammalian erythrocytes are highly specialized cells that have adapted to lose their nuclei and cellular components during maturation to ensure oxygen delivery. Nuclear extrusion, the most critical event during erythropoiesis, represents an extreme case of asymmetric partitioning that requires a dramatic reorganization...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28582
更新日期:2019-11-01 00:00:00