Abstract:
:A factor, termed neutrophil alkaline phosphatase-inducing factor (NAP-IF), that has the capacity to increase the NAP activity of granulocytes was characterized by using two samples: cystic fluid (CF) and conditioned medium of a tumor cell line (T3M5). The molecular weight of NAP-IF was shown to be between 13,000 and 45,000, and its isoelectric point was between 5.5 and 6.2. It was sensitive to heat and proteolytic enzymes, but was resistant to DNase and RNase, suggesting that NAP-IF is an acidic protein or glycoprotein. These characteristics of NAP-IF seem to be similar to those of granulocyte-macrophage colony-stimulating factor (GM-CSF) that is also present in the CF. NAP-IF rich fractions obtained by isoelectric focusing from CF were also found to be rich in a subclass of GM-CSF: granulocyte-CSF (G-CSF). Furthermore, a high correlation was noted between the activities of G-CSF and NAP-IF (gamma = 0.798, P less than 0.005). These results suggest that the two activities, i.e., G-CSF and NAP-IF, may be attributable to an identical macromolecule.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Sato N,Asano S,Mori M,Ueyama Y,Sugano K,Ohsawa N,Takaku Fdoi
10.1002/jcp.1041240213subject
Has Abstractpub_date
1985-08-01 00:00:00pages
255-60issue
2eissn
0021-9541issn
1097-4652journal_volume
124pub_type
杂志文章abstract::Increased vascular resistance in the fetoplacental circulation is a characteristic of preeclampsia. However, the potential molecular mechanisms of this condition remain obscure. The current study aimed to determine the direct effect of the peptide antigen corresponding to the second extracellular loop of the angiotens...
journal_title:Journal of cellular physiology
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abstract:OBJECTIVE:This study aims to investigate the effect of polo-like kinase 1 (PLK1) inhibition on cisplatin (DDP)-resistant gastric cancer (GC) cells. METHODS:The transcriptional level of PLK1 was measured by quantitative reverse-transcription polymerase chain reaction. Expressions of PLK1 and its downstream mediators as...
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更新日期:1981-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1987-02-01 00:00:00
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更新日期:1988-04-01 00:00:00