Abstract:
:Evidence is emerging that long noncoding RNAs (lncRNAs) play vital roles in tumorigenesis. LncRNA gastric cancer-associated transcript 3 (GACAT3) is reported to participate in the development of breast cancer, colorectal cancer, nonsmall cell lung cancer, and gastric cancer. However, whether it is implicated in glioma has not been elucidated. Here, we found that GACAT3 level was aberrantly elevated in glioma tissues and cell lines. Higher GACAT3 expression predicted lower survival rate. Knockdown of GACAT3 suppressed the proliferation, colony formation, migration, and invasion but promoting apoptosis in glioma cells. Next, we determined that GACAT3 contributes to glioma progression through inhibiting microRNA (miR)-3127-5p. Subsequently, ELAVL1 was identified as a direct target of miR-3127-5p by bioinformatics analysis and luciferase reporter assay. Moreover, we confirmed that GACAT3 promoted ELAVL1 expression through sponging miR-3127-5p, leading to glioma progression. Taken together, our study elucidated that GACAT3/miR-3127-5p/ELAVL1 signaling regulates glioma development and might be a promising therapeutic target.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Pan B,Zhao M,Xu Ldoi
10.1002/jcp.27542subject
Has Abstractpub_date
2019-06-01 00:00:00pages
8825-8833issue
6eissn
0021-9541issn
1097-4652journal_volume
234pub_type
杂志文章abstract::Osteoporosis, arthritis, Peget's disease, bone tumor, periprosthetic joint infection, and periprosthetic loosening have a common characteristic of osteolysis, which is characterized by the enhanced osteoclastic bone resorptive function. At present, the treatment target of these diseases is to interfere with osteoclast...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27888
更新日期:2019-07-01 00:00:00
abstract::Glioblastoma (GBM) is the most common, malignant, and deadly primary glioma. Six-transmembrane epithelial antigen of prostate (STEAP) family is involved in tumorigenesis; here, we have explored the biological function and the prognostic value of the STEAP family in GBM. Differentially expressed STEAP genes in tumor an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30060
更新日期:2020-09-23 00:00:00
abstract::Using S1 nuclease protection assays, we have examined the representation of cell cycle-dependent H4 histone RNAs in the nuclear matrix and nonmatrix nuclear fractions of human cells. Cytoplasmic and nuclear fractions were prepared from exponentially growing HeLa S3 cells by double detergent (sodium deoxycholate and NP...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041280229
更新日期:1986-08-01 00:00:00
abstract::The present study was performed to clarify second messenger signaling in parathyroid hormone (PTH)-induced c-fos gene expression, to characterize the participation of the c-fos gene in the regulation of osteoblast proliferation and function as well as osteoclast-like cell formation by PTH and to compare these effects ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041610221
更新日期:1994-11-01 00:00:00
abstract::Osteoporosis is a class of metabolic bone disease caused by complexed ramifications. Overactivation of osteoclasts due to a sudden decreased estrogen level plays a pivotal role for postmenopausal women suffering from osteoporosis. Therefore, inhibiting osteoclast formation and function has become a major direction for...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28289
更新日期:2019-02-27 00:00:00
abstract::The current need for bone grafts in orthopedic and reconstructive surgery cannot be satisfied by autologous tissue transplant due to its limited availability and significant associated morbidity. Tissue engineering approaches could supply sufficient amounts of bone substitutes by exploiting the ability to harvest auto...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.22313
更新日期:2010-11-01 00:00:00
abstract::A decline in cell surface gamma-glutamyl transpeptidase specific activity was previously observed to be concomitant with C6 glial cell proliferation. To elucidate the underlying factor(s) mediating gamma-glutamyl transpeptidase down-regulation, the effects of C6 cell density and culture conditions on cell surface tran...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041500115
更新日期:1992-01-01 00:00:00
abstract::Insulin gene expression in rat insulinoma (RIN) cells is extinct in RIN x fibroblast hybrids and can reappear upon loss of DNA contributed by the fibroblast parent. (Besnard et al., Exp. Cell Res. 185:101-108, 1989). In the present study, we looked for the role of 5'-flanking sequences of the human insulin gene in the...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041460303
更新日期:1991-03-01 00:00:00
abstract::Aberrant glycosylation changes on many glycoproteins are often related to cancer progression and metastasis. sp2 -Iminosugar-type castanospermine analogues, inhibitors of α-glucosidases, have been reported to exhibit antitumor activity. However, their effects on cell migration and the underlying molecular mechanism ar...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25832
更新日期:2017-12-01 00:00:00
abstract::Elevated oxidative stress plays a key role in diabetes-associated vascular disease. In this study, we tested the hypothesis that high glucose-induced oxidative stress was associated with changes in the expression of NADPH oxidase, superoxide dismutase (SOD) and endothelial nitric oxide synthase (eNOS). Oxidative stres...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21063
更新日期:2007-09-01 00:00:00
abstract::The purpose of this study was to delineate the traffic patterns of EGF and EGF receptors (EGFR) in primary cultured acinar epithelial cells from rabbit lacrimal glands. Uptake of [(125)I]-EGF exhibited saturable and non-saturable, temperature-dependent components, suggesting both receptor-mediated and fluid phase endo...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10458
更新日期:2004-04-01 00:00:00
abstract::The role of cyclic adenosine monophosphate (cAMP) is poorly understood in the regulation of normal and abnormal hepatic cell growth. In this study, we examined the regulation of intracellular cAMP levels and its effect on nuclear cAMP responsive elements (CREs) in a rat model of hepatocellular carcinoma (HCC). Tumorig...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20474
更新日期:2006-02-01 00:00:00
abstract::Cell fusion occurs during fertilization and in the formation of organs such as muscles, placenta, and bones. We have developed an experimental model for epithelial cell fusion which permits analysis of the processes during junction disintegration and formation of polykaryons (Palovuori and Eskelinen [2000] Eur. J. Cel...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20024
更新日期:2004-09-01 00:00:00
abstract::It has been established that syndecan-1 is an important modulator of events relevant to acute tissue repair and chronic injury responses. The current studies were designed to examine syndecan-1 expression during atherosclerotic lesion formation and whether angiotensin II influences syndecan-1 expression in macrophages...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21271
更新日期:2008-03-01 00:00:00
abstract::Heat shock factors (HSFs) are the main transcriptional regulators of the stress-induced expression of heat shock protein genes. HSF2, which is one of the HSFs, is activated during differentiation and development but it is unclear how they regulate during cellular processes. Here, we examined the role of HSF4a on the r...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22948
更新日期:2012-01-01 00:00:00
abstract::Mutant forms of the p53 gene have been shown to cooperate with an activated ras gene in transforming primary cells in culture. The aberrant proteins encoded by p53 mutants are thought to act in a dominant negative manner in these assays. In vivo data, however, reveal that where p53 has undergone genetic change in tumo...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041480309
更新日期:1991-09-01 00:00:00
abstract::The extracellular matrix (ECM) is present in a range of molecular conformations and intermolecular arrangements. Fibronectin (Fn) molecules that constitute fibers within the ECM can exist in a variety of conformations that result from both mechanical stress and chemical factors such as allosteric binding partners. The...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25271
更新日期:2016-08-01 00:00:00
abstract::Tenascin-C, a six-armed extracellular matrix glycoprotein, is expressed in a temporally and spatially restricted pattern during carcinogenesis and invasion or metastasis of carcinoma cells in association with stromal-epithelial interactions. The human epidermoid carcinoma-derived cell lines, A431 and HEp-2, which do n...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041650104
更新日期:1995-10-01 00:00:00
abstract::Incubation of isolated rat soleus and EDL muscles in the presence of 10 mM leucine resulted in a decreased proteolytic rate as measured by the release of tyrosine into the incubation medium. The effect of this branched-chain amino acid (BCAA) is associated with a decreased activity of the lysosomal proteases and a dec...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/1097-4652(200009)184:3<380::AID-JCP13>3.0.
更新日期:2000-09-01 00:00:00
abstract::Intermediate subviral particles (ISVP) derived from reovirus represent a simple model system for the switch-on of transcriptase function. In such particles the endogenous transcriptase is present in a switched-off form, one step removed from the switched-on state. Switch-on of transcriptase function is an active proce...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041120104
更新日期:1982-07-01 00:00:00
abstract::Expression of plasminogen activator inhibitor type-1 (PAI-1), a member of the SERPIN gene family that functions to regulate the plasmin-based pericellular proteolytic cascade, is growth state-regulated in normal rat kidney (NRK) cells (Ryan and Higgins, 1990, J. Cell. Physiol., 155:376-384; Ryan et al., 1996, Biochem....
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199701)170:1<8::AID-JCP2>3
更新日期:1997-01-01 00:00:00
abstract::Responses of rat submandibular acini to intracellular alkalinization were investigated. Intracellular alkalinization was induced by addition of NH4Cl or methyl amines, or by prepulse with Na butyrate. Only partial recovery occurred following Na butyrate prepulse or methylated amine addition, but full recovery was obse...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041590310
更新日期:1994-06-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one of the most common malignancies and is a serious threat to people's health worldwide. The prognosis of advanced HCC is dim if left untreated. In the clinic, the treatment options for advanced HCC include surgery, radiotherapy, transcatheter arterial chemoembolization, and so forth...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28048
更新日期:2019-08-01 00:00:00
abstract::Nucleoside transporter proteins are specialized proteins that mediate the transport of nucleosides and nucleoside analog drugs across the plasma membrane. The human equilibrative nucleoside transporter 1 (hENT1) is a member of these proteins and mediates cellular entry of gemcitabine, cytarabine, and fludarabine. The ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22045
更新日期:2010-05-01 00:00:00
abstract::rac-1-O-Myristoyl-2-O-acetylglycerol, rac-1-O-palmitoyl-2-O-acetylglycerol, and rac-1-O-oleoyl-2-O-acetylglycerol acted like phorbol myristate acetate and mezerein in stimulating human neutrophil aggregation. Responses to these agents were equally influenced by cytochalasin B, extracellular calcium and magnesium, arac...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041250204
更新日期:1985-11-01 00:00:00
abstract::Embryo implantation is essential for normal pregnancy, and the process of decidualization is critical for embryo implantation. However, the mechanism of decidualization during early pregnancy is still unknown. Forkhead box O3a (FOXO3a) is the most important functional transcription factor of the forkhead box family an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27167
更新日期:2019-04-01 00:00:00
abstract::The plasma membrane of erythrocytes, as of other cells, is thought to act as the barrier responsible for maintaining intracellular gradients of most ions and small molecular species between the cell and its environment. Controlled application of the nonionic detergent Brij 58 effectively opened the erythrocyte plasma ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041590202
更新日期:1994-05-01 00:00:00
abstract::The present study uses the osteoclast precursor clonal line, HD-11EM, to study the potential of hydrogen peroxide (H2O2) in mediating the differentiation of HD-11EM into osteoclast-like cells. HD-11EM cells are a newly established clonal cell line that, in response to 1alpha,25-(OH)2D3, differentiate into osteoclast-l...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199809)176:3<574::AID-JCP1
更新日期:1998-09-01 00:00:00
abstract::Runx2 and Runx3 are known to be expressed in the growth plate during endochondral bone formation. Here we addressed the functional role of Runx3 as distinct from Runx2 by using two models of postnatal bone repair: fracture healing that proceeds by an endochondral process and marrow ablation that proceeds by only an in...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24396
更新日期:2013-11-01 00:00:00
abstract::Conditions have been established under which the antizyme of ornithine decarboxylase (E.C. 4.1.1.17, L-ornithine carboxy-lyase, ODC) a non-competitive protein inhibitor of ODC, can be detected in cells in response to as little as 10(-7) M putrescine. The maintenance of intracellular antizyme activity depends upon the ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041070206
更新日期:1981-05-01 00:00:00