Abstract:
:Incubation of isolated rat soleus and EDL muscles in the presence of 10 mM leucine resulted in a decreased proteolytic rate as measured by the release of tyrosine into the incubation medium. The effect of this branched-chain amino acid (BCAA) is associated with a decreased activity of the lysosomal proteases and a decreased expression of the genes of the ATP-ubiquitin-dependent proteolysis (ubiquitin and C8). Incubation of muscles in the presence of actinomycin D revealed that the effects of the amino acid can be accounted for by an inhibition of the transcription rate. The presence of leucine did not influence the gene expression of other nonlysosomal (m-calpain) and lysosomal (cathepsin B) proteolytic systems. It is concluded that the well-known effect of BCAA on muscle proteolysis is mediated, in the short term, by the inhibition of lysosomal proteolysis. In a longer period, based on the inhibition of gene transcription observed, an involvement of the ATP-dependent proteolytic system is also likely to occur.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Busquets S,Alvarez B,Llovera M,Agell N,López-Soriano FJ,Argilés JMdoi
10.1002/1097-4652(200009)184:3<380::AID-JCP13>3.0.keywords:
subject
Has Abstractpub_date
2000-09-01 00:00:00pages
380-4issue
3eissn
0021-9541issn
1097-4652pii
10.1002/1097-4652(200009)184:3<380::AID-JCP13>3.0.journal_volume
184pub_type
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