Abstract：
:Radioiodinated granulocyte-macrophage colony-stimulating factor (125I-GM-CSF) binds to specific receptors (molecular weight approximately 50,000 daltons) on the murine myelomonocytic leukemia, WEHI-3BD+. At 4 degrees C 125I-GM-CSF remains on the surface of the cells and can be eluted by washing the cells with acidified isotonic buffer. When the cells are warmed to 37 degrees C, the 125I-GM-CSF is internalized rapidly (t 1/2: 7 min). The internalisation appears to be entirely receptor mediated and is independent of energy sources inhibited by sodium azide. This GM-CSF-mediated internalisation is not due to a general increase in the turnover of cell surface molecules as the specific binding of 125I-transferrin is not affected by incubation of WEHI-3BD+ cells with GM-CSF. The initial 125I released when the cells are warmed to 37 degrees C appears to be intact 125I-GM-CSF; however, after 2 h 80% of the 125I released was not precipitable with trichloroacetic acid and presumably represented degraded 125I-GM-CSF. Ammonium chloride or monensin reduced the release of 125I-GM-CSF from the cells, suggesting that the receptor-bound ligand was processed through the lysosomes. A considerable proportion of the internalised GM-CSF receptors were recycled to the surface and were available for ligand binding. Synthesis of new GM-CSF receptors contributed to the re-expression of GM-CSF receptors after down-regulation and it is possible that the GM-CSF enhances the synthesis of its own receptors.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Walker F,Burgess AWdoi
10.1002/jcp.1041300211subject
Has Abstractpub_date
1987-02-01 00:00:00pages
255-61issue
2eissn
0021-9541issn
1097-4652journal_volume
130pub_type
杂志文章abstract：:Islets of Langerhans control whole body glucose homeostasis, as they respond, releasing hormones, to changes in nutrient concentrations in the blood stream. The regulation of hormone secretion has been the focus of attention for a long time because it is related to many metabolic disorders, including diabetes mellitus...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.25176
更新日期：2016-04-01 00:00:00
abstract：:Acute myeloid leukemia (AML) is the most common type of leukemia in adults. AML cells secrete angiogenic factors to remodel vasculature and acquire chemoresistance; however, antiangiogenic drugs are often ineffective in AML treatment. Cancer cell-derived exosomes can induce angiogenesis, but their role in vascular rem...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27735
更新日期：2019-07-01 00:00:00
abstract：:The retinoblastoma protein-interacting zinc-finger (RIZ) gene, also known as PRDM2, encodes two protein products, RIZ1 and RIZ2, differing for the presence of a 202 aa domain, called PR domain, at the N-terminus of the RIZ1 molecule. While the histone H3 K9 methyltransferase activity of RIZ1 is associated with the neg...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22803
更新日期：2012-03-01 00:00:00
abstract：:Vole cells transformed by avian sarcoma virus carrying the src gene lose their fibroblastic morphology, the organized cytoskeletal system of the normal fibroblastic cell, the typical fibronectin deposit around the cell membrane, and the ability to shut off multiplication when suspended in liquid medium. All of these t...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041070312
更新日期：1981-06-01 00:00:00
abstract：:The mechanisms of agonist-induced calcium entry (ACE) following depletion of intracellular calcium stores have not been fully established. We report here that calcium-independent phospholipase A (iPLA(2)) is required for robust Ca(2+) entry in HaCaT keratinocytes following ATP or UTP stimulation. Lysophosphatidic acid...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20993
更新日期：2007-06-01 00:00:00
abstract：:miR-222 participates in many cardiovascular diseases, but its effect on cardiac remodeling induced by diabetes is unclear. This study evaluated the functional role of miR-222 in cardiac fibrosis in diabetic mice. Streptozotocin (STZ) was used to establish a type 1 diabetic mouse model. After 10 weeks of STZ injection,...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29119
更新日期：2020-03-01 00:00:00
abstract：:The proliferation of most cells is strictly dependent on cell-matrix interactions, a phenomenon called anchorage dependence. Because tumor cells are often independent of this regulation, it is important to characterize the molecular components that are involved in this control. We therefore investigated a possible rol...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(200001)182:1<88::AID-JCP10
更新日期：2000-01-01 00:00:00
abstract：:Retinoic acid (RA) reduced growth, fibronectin, and retinoic acid receptor (RAR alpha) in NIH 3T3 cells but not in cells transformed by the Ha-ras oncogene. RA lowered RAR alpha transcript and protein, increased RAR beta transcripts, and had no effect on RAR gamma. H-ras transformation downregulated RAR expression and...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199711)173:2<297::AID-JCP3
更新日期：1997-11-01 00:00:00
abstract：:Splenic erythroblasts obtained from mice during the acute disease caused by either the polycythemia-inducing (FVP) or anemia-inducing (FVA) strain of Friend virus were examined for their degree of terminal differentiation. Morphology, benzidine staining, and heme synthesis kinetics showed that many erythroblasts from ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041210311
更新日期：1984-12-01 00:00:00
abstract：:Leukocyte accumulation has been shown to be increased in sepsis. Moreover, in inducible nitric oxide synthase (iNOS) knockout mice, a further increase in leukocyte accumulation has been observed during sepsis, suggesting that nitric oxide (NO) may affect leukocyte/endothelial interaction. Accelerated peroxynitrite for...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1128
更新日期：2001-09-01 00:00:00
abstract：:Gastric cancer has the fifth highest incidence of disease and is the third leading cause of cancer-associated mortality in the world. The etiology of gastric cancer is complex and needs to be fully elucidated. Thus, it is necessary to explore potential pathogenic genes and pathways that contribute to gastric cancer. G...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29177
更新日期：2020-03-01 00:00:00
abstract：:Runx2 and Runx3 are known to be expressed in the growth plate during endochondral bone formation. Here we addressed the functional role of Runx3 as distinct from Runx2 by using two models of postnatal bone repair: fracture healing that proceeds by an endochondral process and marrow ablation that proceeds by only an in...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24396
更新日期：2013-11-01 00:00:00
abstract：:Osteosarcoma (OS) is an ultra-rare highly malignant tumor of the skeletal system affecting mainly children and young adults and it is characterized by an extremely aggressive clinical course. OS patients are currently treated with chemotherapy and complete surgical resection of cancer tissue. However, resistance to ch...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26330
更新日期：2018-06-01 00:00:00
abstract：:Acute lung injury (ALI) is a severe acute inflammatory reaction of the lungs caused by a variety of factors, which can lead to a high mortality rate. MicroRNAs are a novel therapeutic molecule that play a vital role in many diseases. However, its mechanism of action in lipopolysaccharide (LPS)-induced mouse ALI is not...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28850
更新日期：2019-12-01 00:00:00
abstract：:Polycystin-1 regulates a number of cellular processes through the formation of complexes with the polycystin-2 ion channel or with other signal transduction proteins. Polycystin-1 is expressed in many tissues but other members of this gene family are distributed in a more restricted fashion. PKDREJ expression has been...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20755
更新日期：2006-11-01 00:00:00
abstract：:Nerve growth factor (NGF) is required for the growth and development of sensory and sympathetic neurons. Incubation of chick dorsal root ganglionic cells without NGF resulted in a decrease of active (Na+,K+-pump-mediated) K+ influx over a period of several hours. Addition of NGF to NGF-deprived cells caused 1) a retur...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041130107
更新日期：1982-10-01 00:00:00
abstract：:Syndecan-1 belongs to a family of transmembrane proteoglycans, acts as a coreceptor for growth factor binding, as well as cell-matrix and cell-cell interactions, and is induced in smooth muscle cells (SMCs) following balloon catheter injury. In this report, we investigated syndecan-1 expression in SMCs in response to ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20927
更新日期：2007-04-01 00:00:00
abstract：:Simian Virus 40 (SV40), a monkey polyomavirus, was administered to human populations by early anti-poliomylitis vaccines contaminated by this small DNA tumor virus. Data on SV40 infection in humans remain controversial. Elderly subjects represent an interesting cohort to investigate, because they were not immunized wi...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25405
更新日期：2017-01-01 00:00:00
abstract：:MCOLN1 encodes mucolipin-1 (TRPML1), a member of the transient receptor potential TRPML subfamily of channel proteins. Mutations in MCOLN1 cause mucolipidosis-type IV (MLIV), a lysosomal storage disorder characterized by severe neurologic, ophthalmologic, and gastrointestinal abnormalities. Along with TRPML1, there ar...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21956
更新日期：2010-02-01 00:00:00
abstract：:The smooth muscle cell population in major arteries of humans and experimental animals is heterogeneous with regard to cellular DNA content. A proportion of cells has polyploid DNA content and this proportion increases with normal aging and with hypertension. We have isolated pure populations of rat aortic smooth musc...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041250322
更新日期：1985-12-01 00:00:00
abstract：:In vitro incubation of hepatocytes acutely isolated from adult male rats leads to a rapid conversion of the adrenergic activation of glycogenolysis from an alpha 1-receptor (alpha 1AR) to a beta 2-receptor (beta 2AR) mediated response within 4 h. In order to understand the underlying mechanism, we examined time-depend...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041520111
更新日期：1992-07-01 00:00:00
abstract：:Wnt/β-catenin signaling pathway plays a crucial role in diabetic cardiomyopathy (DCM), thus we aimed at investigating the effect of one therapeutic approach with resveratrol (RSV) given systemically and combined treatment of RSV with mesenchymal stem cells (MSCs) that was either RSV-preconditioned or not on Wnt/β-cate...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27947
更新日期：2019-07-01 00:00:00
abstract：:We report here that the androgen receptor (AR) and ABCB1 are upregulated in a model of acquired taxol resistance (txr) in ovarian carcinoma cells. AR silencing sensitizes txr cells to taxol threefold, whereas ectopic AR expression in AR-null HEK293 cells induces resistance to taxol by 1.7-fold. AR activation using the...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27535
更新日期：2019-06-01 00:00:00
abstract：:Alkaptonuria (AKU) is an ultra-rare genetic disease, in which the accumulation of a toxic metabolite, homogentisic acid (HGA) leads to the systemic development of ochronotic aggregates. These aggregates cause severe complications mainly at the level of joints with extensive degradation of the articular cartilage. Prim...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25761
更新日期：2017-11-01 00:00:00
abstract：:Rous sarcoma virus (RSV)-infected chicken embryo cells were used to study the effect of viral transformation on the hormone-stimulated synthesis of cyclic AMP. Transformation by RSV greatly increased the cells' ability to synthesize and accumulate cyclic AMP in response to the beta-adrenergic agonist isoproterenol as ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041110311
更新日期：1982-06-01 00:00:00
abstract：:Asthma is the most common respiratory disease accompanied by lung inflammatory disorders. The main symptoms are airway obstruction, chronic inflammation due to mast cell and eosinophil activity, and the disturbance of immune responses mostly mediated by the Th2 response. Genetic background and environmental factors al...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.28781
更新日期：2019-12-01 00:00:00
abstract：:The actions of prostaglandins (PG) on cAMP in dispersed chief cells from guinea pig stomach were examined and compared to the actions of these agents on pepsinogen secretion. Maximal concentrations of A, B, or E prostaglandins caused a 2-5-fold increase in pepsinogen secretion and cellular cAMP. The relative order of ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041320119
更新日期：1987-07-01 00:00:00
abstract：:Basic fibroblast growth factor (bFGF, FGF-2) is progressively lost from mammary epithelial cells as they become malignant. To investigate the effects of restoring the expression of bFGF in breast cancer cells, we constructed MCF-7 cells that permanently overexpress 18-kD cytoplasm-localizing bFGF (MCF-7/deltaA(FGF)(18...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199812)177:3<411::AID-JCP5
更新日期：1998-12-01 00:00:00
abstract：:Clinicians have observed that keloids preferentially form in body areas subject to increased skin tension. We hypothesized a difference exists in the transcriptional response of keloid fibroblasts to mechanical strain compared with normal fibroblasts. Normal and keloid fibroblasts were seeded in a device calibrated to...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20486
更新日期：2006-02-01 00:00:00
abstract：:Respiratory tract epithelia are one type of tissue targeted by vitamin A. In this study the effects of vitamin A and its analogs (retinoids) on human bronchial epithelial (HBE) cells have been investigated in a serum-free hormone-supplemented medium. This serum-free medium, which was developed for the long-term cultiv...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041270110
更新日期：1986-04-01 00:00:00
