Abstract:
:Adhesion of human monocytes and granulocytes to vascular endothelium plays an important role in migration of these cells to inflammatory sites in tissues. A family of three human leukocyte heterodimeric surface molecules named Mo1, LFA-1, and p150,95 (LeuM5) has been shown to mediate leukocyte adhesion to confluent monolayers of human umbilical vein endothelial cells (HUVE). The relative contribution of each of the three molecules in leukocyte endothelial adhesion was studied using a variety of stimuli. Purified human granulocytes and monocytes were radiolabelled and incubated with HUVE for 45 minutes in a 37 degrees C humidified 5% CO2 incubator in the presence or absence of subunit-specific monoclonal antibodies (MAbs). Adhesion was assessed by quantitation of endothelial cell-associated radioactivity and confirmed by microscopic evaluation. MAbs directed against the alpha subunit of LFA-1 as well as to the beta subunit common to all three antigens significantly inhibited unstimulated monocyte adhesion to HUVE. Small but significant inhibiton was also observed using MAbs directed against Mo1a and p150. Phorbol myristate acetate (PMA)-induced grranulocyte adhesion to HUVE was significantly inhibited by anti-Mo1a and anti-beta, but not by anti-LFA-1a or anti-p150. When HUVE were prestimulated by recombinant IL-1, a different pattern of antigen utilization by granulocytes was observed. MAbs directed against each of the three alpha subunits as well as the common beta subunit all inhibited granulocyte adhesion to HUVE. Furthermore the effect of the three anti-alpha subunit MAbs on granulocyte-HUVE adhesion was additive. These studies show that relative contribution of Mo1, LFA-1, and p150,95 to leukocyte endothelial adhesion varies depending on the cell type and the stimulus used. These studies also reveal a novel role for p150,95 in promoting monocyte and granulocyte adhesion to HUVE.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Arnaout MA,Lanier LL,Faller DVdoi
10.1002/jcp.1041370214subject
Has Abstractpub_date
1988-11-01 00:00:00pages
305-9issue
2eissn
0021-9541issn
1097-4652journal_volume
137pub_type
杂志文章abstract::Experiments were conducted to determine the extent and variability of collagen degradation in human fetal lung fibroblasts. Cells were incubated with [14C]proline, and degradation was measured by determining the hydroxy[14C]proline in a low molecular weight fraction relative to total hydroxy[14C]proline. Average (basa...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041210119
更新日期:1984-10-01 00:00:00
abstract::Relative to their normal counterparts, transformed epithelial cells have a distinctive and quantifiable three-dimensional shape. Biophysical and mathematical methods are used to distinguish these extremes in cells from two lines, cultured from rat liver and tracheal epithelium, respectively. Cells adopted a more trans...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199601)166:1<217::AID-JCP2
更新日期:1996-01-01 00:00:00
abstract::Versican is a large chondroitin sulfate proteoglycan and belongs to the family of lecticans. Versican possesses two globular domains, G1 and G3 domain, separated by a CS-attachment region. The CS-attachment region present in the middle region is divided into two spliced domains named CSalpha and beta. Alternative spli...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20921
更新日期:2007-04-01 00:00:00
abstract::We have investigated the role of the plasminogen activation cascade in skeletal muscle differentiation. Migrating, undifferentiated myoblasts express urokinase plasminogen activator (uPA) and its cell surface receptor (uPAR). Consequently, uPA is localized predominantly to the cell surface. Preventing uPA from associa...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199705)171:2<217::AID-JCP1
更新日期:1997-05-01 00:00:00
abstract::We have characterized the physiological and biochemical properties of the Na(+)/K(+) pump and its molecular expression in L8 rat muscle cells. Pump properties were measured by [(3)H]ouabain binding and (86)Rb uptake. Scatchard plot analysis of specific ouabain binding indicated the presence of a single family of bindi...
journal_title:Journal of cellular physiology
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更新日期:2001-06-01 00:00:00
abstract::Hepatic expression of the protooncogenes c-fos and c-myc occurs within 2 h after partial hepatectomy, and these immediate early genes are thought to prime the hepatocytes for subsequent proliferation. To examine whether such gene activation occurred in the setting of hepatocyte proliferation after toxic liver injury, ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041540212
更新日期:1993-02-01 00:00:00
abstract::Induction of apoptosis has been associated with a variety of exposures which result in inflammatory and fibrotic lung disorders. Macrophages are key regulatory cells in the lung; however, the role of apoptotic macrophages in those pulmonary disorders is not well characterized. In the present investigation, apoptotic m...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
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更新日期:2017-12-01 00:00:00
abstract::An increasing body of evidence shows that many of the key inositol lipids and enzymes responsible for their metabolism reside in nuclei. Moreover, the association of the nuclear phosphoinositide cycle with progression through the cell cycle and commitment toward differentiation has built a wider picture of the implica...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-4652(199911)181:2<203::AID-JCP3
更新日期:1999-11-01 00:00:00
abstract::Cation-Cl- cotransporters (CCCs) belong to a large family of proteins that includes 9 isoforms, two of which have still not been ascribed a transport function (CCC8 and CCC9) while the others are all known to promote Cl(-)-coupled Na+ and/or K+ movement at the cell surface. The CCCs are also included in a larger famil...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21814
更新日期:2009-09-01 00:00:00
abstract::Chronic myeloid leukemia (CML) is a hematopoietic stem cell disorder caused by the oncogenic activity of the Bcr-Abl protein, a deregulated tyrosine kinase. Calcium may act directly on cellular enzymes and in conjunction with other cellular metabolites, such as cyclic nucleotides, to regulate cell functions. Alteratio...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22140
更新日期:2010-08-01 00:00:00
abstract::Ischemic stroke (IS), which is characterized by high morbidity, disability, and mortality, is recognized as a major cerebrovascular disease. MicroRNA-31 (miR-31) was reported to participate in the progression of brain disease. The present study was conducted in order to investigate the effect of miR-31 on oxidative st...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,收录出版
doi:10.1002/jcp.29146
更新日期:2020-03-01 00:00:00
abstract::Wound healing is critically affected by age, ischemia, and growth factors such as TGFbeta1. The combined effect of these factors on fibroblast migration, an essential component of wound healing, is poorly understood. To address this deficiency, we examined expression of TGFbeta receptor type I and II (TGFbetaRI and RI...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10060
更新日期:2002-02-01 00:00:00
abstract::The exposure to non-thermal microwave electromagnetic field (MW-EMF) at 1.95 MHz, a frequency used in mobile communication, affects the refolding kinetics of eukaryotic proteins (Mancinelli et al., 2004). On these basis we have evaluated the in vivo effect of MW-EMF in human epidermoid cancer KB cells. We have found t...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20327
更新日期:2005-08-01 00:00:00
abstract::Reactivation of dormant chick erythrocyte (CE) nuclei was studied by fusing chick red cells with rat myoblasts, HeLa cells, and chick fibroblasts. Heterokaryons representing different stages of nuclear reactivation were fixed and examined for nuclear antigens using polyclonal patient autoantisera reacting with mammali...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041200221
更新日期:1984-08-01 00:00:00
abstract::High potassium concentrations altered the morphology and the ability to grow in soft agar in 6m2 cells, a clone of rat kidney cells infected with a temperature-sensitive mutant of Moloney sarcoma virus. Approximately 60% of cells exhibited normal morphology in the presence of 94.8 mM potassium in isotonic medium at th...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041250213
更新日期:1985-11-01 00:00:00
abstract::The activation/adaptive responses of human monocytes exposed to Bordetella pertussis parental or mutant strains were evaluated and correlated to the expression of two bacterial toxins: adenylate cyclase-hemolysin and pertussis toxin. The marked rise in intracellular cyclic adenosine monophosphate (cAMP) observed in mo...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(200004)183:1<91::AID-JCP11
更新日期:2000-04-01 00:00:00
abstract::The potential usage of curcumin in diverse human diseases has been widely studied, including arteriosclerosis (AS). This study focused on investigating the relationship between curcumin and AS-associated microRNA, which may provide a better understanding of curcumin in a different mechanism. Human microvascular endoth...
journal_title:Journal of cellular physiology
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doi:10.1002/jcp.28708
更新日期:2019-11-01 00:00:00
abstract::Tfcp2l1 is a transcription factor critical for mouse embryonic stem cell (mESC) maintenance. However, its role in human ESCs (hESCs) remains unclear. Here, we investigated the role of Tfcp2l1 in controlling hESC activity and showed that Tfcp2l1 is functionally important in the maintenance of hESC identity. Tfcp2l1 exp...
journal_title:Journal of cellular physiology
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更新日期:2018-09-01 00:00:00
abstract::Hyperammonaemia and hyperethanolaemia are thought to be driving factors behind skeletal muscle myopathy in liver disease, that is, cirrhosis. Despite this, the singular and combined impacts of ethanol- and ammonia-induced protein catabolism are poorly defined. As such, we aimed to dissect out the effects of ammonia an...
journal_title:Journal of cellular physiology
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abstract::Adult stem cell therapy is being used extensively to rejuvenate damaged tissue. One important tissue source to obtain these cells is adipose, which contains cells called adipose-derived stem cells (ADSCs). These cells have a great therapeutic potential not only for their multipotent properties as well as for immunomod...
journal_title:Journal of cellular physiology
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abstract::We present evidence that extends our earlier preliminary report on the stimulatory effect of saline washes on confluent cells in culture (Lacey et al., '77). That work suggested that an inhibitory substance was being removed by the washes. The present work suggests that the inhibitor is in the 10,000-30,000 MW range, ...
journal_title:Journal of cellular physiology
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更新日期:1981-02-01 00:00:00
abstract::The aim of this study was to identify the molecules and pathways involved in the cross-talk between meniscus and synovium that may play a critical role in osteoarthritis (OA) pathophysiology. Samples of synovium and meniscus were collected from patients with early and end-stage OA and cultured alone or cocultured. Cyt...
journal_title:Journal of cellular physiology
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abstract::Tenascin-C, a six-armed extracellular matrix glycoprotein, is expressed in a temporally and spatially restricted pattern during carcinogenesis and invasion or metastasis of carcinoma cells in association with stromal-epithelial interactions. The human epidermoid carcinoma-derived cell lines, A431 and HEp-2, which do n...
journal_title:Journal of cellular physiology
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更新日期:1995-10-01 00:00:00
abstract::Oxidized-low density lipoprotein (Ox-LDL) has been shown to play an important role in impaired surfactant metabolism and transforming growth factor-β1 (TGF-β1) is a critical mediator in the pathogenesis of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). In this study, we investigated whether Ox-LDL...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24005
更新日期:2012-09-01 00:00:00
abstract::The multiplication rate of sparse cultures of chick embryo cells is only slightly lower at pH 6.9 than at pH 7.4. There is, however, a marked reduction in the multiplication rate of the pH 6.9 cultures before they reach confluency. Cultures at pH 7.4 continue to multiply beyond confluency with only a slight decrease i...
journal_title:Journal of cellular physiology
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更新日期:1975-06-01 00:00:00
abstract::The cellular basis of the membrane-limited state of glucose utilization and the mechanism of the endogenous regulation of hexose uptake in dense monolayers of C6 glioma cells were investigated. In an earlier study, it was shown that at high rates of glucose transport and phosphorylation combined with the inhibition of...
journal_title:Journal of cellular physiology
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abstract::Impaired cardiac control of intracellular diastolic Ca(2+) gives rise to arrhythmias. Whereas exercise training corrects abnormal cyclic Ca(2+) handling in heart failure, the effect on diastolic Ca(2+) remains unstudied. Here, we studied the effect of exercise training on the generation and propagation of spontaneous ...
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journal_title:Journal of cellular physiology
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doi:10.1002/jcp.21339
更新日期:2008-05-01 00:00:00
abstract::Liver regeneration involves not only hepatocyte replication but progenitor aggregation and scarring. Partial hepatectomy (PH), an established model for liver regeneration, reactivates transforming growth factor-β (TGF-β) signaling. Hepatic stellate cells (HSCs) are primarily responding cells for TGF-β and resident in ...
journal_title:Journal of cellular physiology
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