Abstract:
:Theranostic nanoparticles (NPs) are promising for opening new windows toward personalized disease management. Using a single particle capable of both diagnosis and drug delivery, is the major benefit of such particles. In the present study, chitosan NPs were used as a dual action carrier for doxorubicin (DOX; chemotherapeutic agent) and superparamagnetic iron oxide nanoparticles (SPIONs; imaging agent). SPIONs and DOX were loaded at different concentrations within poly-l-arginine-chitosan-triphosphate matrix (ACSD) using the ionic gelation method. NPs' size were in the range of 184.33 ± 4.4 nm. Drug release analysis of DOX loaded NPs (NP-DOX) showed burst release at pH 5.5 (as in tumor environment) and slow release at pH 7.4 (physiological condition), demonstrating pH-sensitive drug release profile. NP-DOX internalization was confirmed by flowcytometry and fluorescent microscopy. Uptake process results were corroborated by accumulation of drug in the intracellular space. Iron content was evaluated by inductively coupled plasma and prussian blue staining. In vitro magnetic resonance imaging (MRI) showed a decline in T 2 relaxation times by increasing iron concentration. MRI analysis also confirmed uptake of NPs at the optimum concentration in C6 glioma cells. In conclusion, ACSD NPs could be utilized as a promising theranostic formulation for both diagnosis and treatment of glioblastoma.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Gholami L,Tafaghodi M,Abbasi B,Daroudi M,Kazemi Oskuee Rdoi
10.1002/jcp.27019subject
Has Abstractpub_date
2019-02-01 00:00:00pages
1547-1559issue
2eissn
0021-9541issn
1097-4652journal_volume
234pub_type
杂志文章abstract::Lung cancer (LC) is the leading cause of cancer-related deaths all over the world, among both men and women, with an incidence of over 200,000 new cases per year coupled with a very high mortality rate. LC comprises of two major clinicopathological categories: small-cell (SCLC) and nonsmall-cell lung carcinoma (NSCLC)...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.26607
更新日期:2018-04-20 00:00:00
abstract::The next-generation sequencing (NGS) based RNA sequencing (RNA-Seq) and transcriptome profiling offers an opportunity to unveil complex biological processes. Successful RNA-Seq and transcriptome profiling requires a large amount of high-quality RNA. However, NGS-quality RNA isolation is extremely difficult from recalc...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25951
更新日期:2018-03-01 00:00:00
abstract::Much research has been conducted to discover novel techniques to reverse the process of tumorigenesis and, cure already stablished malignancies. One well-stablished approach has been the use of organic compounds and naturally found agents such as melatonin whose anticancer effects have been shown in multiple studies, ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28435
更新日期:2019-08-01 00:00:00
abstract::Toll-like receptors (TLRs) are related to foam cell formation (FCF), key event in the establishment/progression of atherosclerosis. The activation of TLR2 and TLR4 can increase FCF. The aim of this study was to evaluate the role of TLR9 in FCF. Murine macrophages were treated with CpG-ODN, TLR9 agonist, and oxidized p...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22022
更新日期:2010-04-01 00:00:00
abstract::Scatchard analysis of binding of 125I-basic fibroblast growth factor (FGF) to baby hamster kidney (BHK) cells revealed the presence of two binding sites: a high affinity site with KD of 20 pM and 80,000 sites per cell and a low affinity site with KD of about 2 nM and 600,000 sites per cell. The binding to the two site...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041310118
更新日期:1987-04-01 00:00:00
abstract::Arg8-vasopressin (AVP) is a potent inducer of myogenic differentiation stimulating the expression of myogenic regulatory factors. To understand the mechanism of its effect on myogenesis, we investigated the early signals induced by AVP in myogenic target cells. In the rat skeletal muscle cell line L6, AVP selectively ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199704)171:1<34::AID-JCP5>
更新日期:1997-04-01 00:00:00
abstract::Salivary dysfunction commonly occurs in many older adults and is considered a physiological phenomenon. However, the genetic changes in salivary glands during aging have not been characterized. The present study analyzed the gene expression profile in salivary glands from accelerated aging klotho deficient mice (kloth...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26172
更新日期:2018-04-01 00:00:00
abstract::The biosynthesis and assembly of the peripheral sector (V1) of the vacuolar proton-translocating adenosine triphosphatase (V-ATPase) was studied in a bovine kidney epithelial cell line. Monolayer cultures of cells were metabolically radiolabeled with Tran35S-label and the V-ATPase subsequently immunoprecipitated using...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041560106
更新日期:1993-07-01 00:00:00
abstract::MCF-10A human mammary epithelial cells cultured inside reconstituted basement membrane form acini that resemble the acinar structures of mammary lobules. This three-dimensional culture system has been used for identifying and characterizing the signal transduction pathways controlling cell proliferation and death, and...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20639
更新日期:2006-07-01 00:00:00
abstract::Exposure of cultured endothelial cells to endotoxin causes an increase in the amount of cellular heparan sulfate proteoglycan and a depletion of this molecule in the extracellular matrix. Concomitant with the decrease in the extracellular matrix is the appearance of a fraction of proteoglycan bearing altered carbohydr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041590116
更新日期:1994-04-01 00:00:00
abstract::The manipulation of autophagy provides a new opportunity for highly effective anticancer therapies. Recently, we showed that photodynamic therapy (PDT) with nitrogen-doped titanium dioxide (N-TiO2 ) nanoparticles (NPs) could promote the reactive oxygen species (ROS)-dependent autophagy in leukemia cells. However, the ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29479
更新日期:2020-11-01 00:00:00
abstract::The S100 protein family comprises more than 20 members of small calcium binding proteins operating as Ca2 +-activated switches that interact and modulate the activity of a large number of targets. S100A1 and S100B, two members of this family, have been recently associated with the differentiation status of human artic...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.25720
更新日期:2017-08-01 00:00:00
abstract::The expression of laminin, a major constituent of endometrial cell basement membranes, is increased during differentiation of human endometrial stromal cells (decidualization). To determine whether laminin plays a role in decidualization, we studied the effects of laminin substrate on the synthesis and release of prol...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041630105
更新日期:1995-04-01 00:00:00
abstract::Repetitive mechanical stimulation of cultured avian skeletal muscle increases the synthesis of prostaglandins (PG) E2 and F2 alpha which regulate protein turnover rates and muscle cell growth. These stretch-induced PG increases are reduced in low extracellular calcium medium and by specific phospholipase inhibitors. M...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041550109
更新日期:1993-04-01 00:00:00
abstract::The involvement of Ras-GTPases in the development of renal fibrosis has been addressed in the last decade. We have previously shown that H- and N-Ras isoforms participate in the regulation of fibrosis. Herein, we assessed the role of K-Ras in cellular processes involved in the development of fibrosis: proliferation, m...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25340
更新日期:2016-10-01 00:00:00
abstract::Past work has shown that delta-opioid receptor (DOR) activation by [D-Ala(2),D-Leu(5)]-enkephalin (DADLE) attenuated the disruption of K(+) homeostasis induced by hypoxia or oxygen-glucose deprivation (OGD) in the cortex, while naltrindole, a DOR antagonist blocked this effect, suggesting that DOR activity stabilizes ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21000
更新日期:2007-07-01 00:00:00
abstract::The addition of anti-laminin IgG to the basal surfaces of rat Sertoli cells in culture in a two-chambered assembly results in a perturbation of F-actin arrangements, including disruption of the pericellular circumferal rings, impairments of the Sertoli cell permeability barrier, and subsequently focal defoliation, fol...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041560102
更新日期:1993-07-01 00:00:00
abstract::Recent evidence has verified the cardioprotective actions of irisin in different diseases models. However, the beneficial action of irisin on hypoxia-reoxygenation (HR) injury under high glucose stress has not been described. Herein our research investigated the influence of irisin on HR-triggered cardiomyocyte death ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29030
更新日期:2020-02-01 00:00:00
abstract::We have analyzed the expression of Na,K-ATPase alpha subunit isoforms in the transporting ciliary processes of the human eye and in cultured cells derived from non-pigmented (NPE) and pigmented (PE) ciliary epithelium. Northern hybridization analysis shows that the mRNAs encoding all the three distinct forms of Na,K-A...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041410203
更新日期:1989-11-01 00:00:00
abstract::The growth of human epithelial cells is stimulated by cholera toxin and analogues of cyclic AMP, while the growth of breast derived fibroblasts is inhibited. These compounds have little effect on DNA synthesis in the absence of other mitogens but show a synergistic effect with serum and/or EGF. The results suggest tha...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041020306
更新日期:1980-03-01 00:00:00
abstract::Zinc finger E-box binding homeobox 1 (ZEB1), an important transcription factor belonging to the ZEB family, plays a crucial role in regulating gene expression required for both normal physiological and pathological processes. Accumulating evidence has shown that ZEB1 participates in the initiation and progression of a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.30177
更新日期:2020-12-04 00:00:00
abstract::Conditions have been established under which the antizyme of ornithine decarboxylase (E.C. 4.1.1.17, L-ornithine carboxy-lyase, ODC) a non-competitive protein inhibitor of ODC, can be detected in cells in response to as little as 10(-7) M putrescine. The maintenance of intracellular antizyme activity depends upon the ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041070206
更新日期:1981-05-01 00:00:00
abstract:BACKGROUND:Pulmonary arterial hypertension (PAH) is characterized by the apoptosis resistance and hyperproliferation of pulmonary artery smooth muscle cells (PASMCs). Its pathogenesis has not been revealed. Here, we carried out experiments to investigate the functions of miR-140-5p and tumor necrosis factor-α (TNF-α). ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27642
更新日期:2019-06-01 00:00:00
abstract::Cholangiocarcinoma (CCA) is a mortal cancer with gradually increasing incidences all over the world, whereas effective diagnosis and treatment for this disease are still lacking. As a classical long noncoding RNA (lncRNA), maternally expressed gene 3 (MEG3) has been reported to exhibit pivotal regulatory roles in the ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28856
更新日期:2019-12-01 00:00:00
abstract::Phospholipase A2 (PLA2) is pivotal in the rapid membrane-mediated actions of 1,25-dihydroxyvitamin D3 [1alpha,25(OH)2D3]. Microarray analysis indicated that PLA2 activating protein (PLAA) mRNA is upregulated 6-fold before rat growth plate cells exhibit 1alpha,25(OH)2D3-dependent protein kinase C (PKC) increases, sugge...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20212
更新日期:2005-04-01 00:00:00
abstract::The level of poly A+ RNA in growing cultures of human diploid fibroblasts is 1.8-fold times greater than in resting cultures. The level of functional ribosomes in growing cultures is 2.8 times that in resting cultures. Since transit times are similar in both types of cells, it can be concluded that the rate of protein...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040940212
更新日期:1978-02-01 00:00:00
abstract::Despite remarkable progress in polychemotherapy protocols, pediatric B-cell acute lymphoblastic leukemia (B-ALL) remains fatal in around 20% of cases. Hence, novel targeted therapies are needed for patients with poor prognosis. Glucocorticoids (GCs) are drugs commonly administrated for B-ALL treatment. Activation of t...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26135
更新日期:2018-03-01 00:00:00
abstract::Deep vein thrombosis associated with advanced cancer is known as Trousseau's syndrome. We hypothesized that thrombin, an activator of protease-activated receptor (PAR)-1 and PAR-4 contributes to tumor metastasis. In this study, we demonstrated that thrombin and the PAR-1 activating peptide (AP) SFLLRN, but not the PAR...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22428
更新日期:2011-04-01 00:00:00
abstract::Peripheral nerve physiology and regeneration has been observed and investigated in literature but surgical applications to reconstruct and restore motor or sensory functions are still in a developmental phase. The peripheral nerve progresses slowly and incompletely compared with other tissues, it may provoke separatio...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.27299
更新日期:2019-04-01 00:00:00
abstract::Highly purified human blood burst-forming units-erythroid (BFU-E) were used to study the effects of interferon gamma (IFN gamma). IFN gamma inhibited erythroid colony formation, cell proliferation, and differentiation of day 3 to day 6 mature BFU-E in a dose-dependent manner. The primitive BFU-E (day 1 and day 2 cells...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041650213
更新日期:1995-11-01 00:00:00