Abstract：
:Heparin and related glycosaminoglycans are potent inhibitors of both in vivo and in vitro smooth muscle cell (SMC) proliferation. We have found that epidermal growth factor (EGF) reverses the antiproliferative effects of heparin. Other known SMC mitogens, including platelet-derived growth factor (PDGF), insulin-like growth factor-1 (IGF-1), and thrombin, were unable to prevent heparin action. The EGF specificity was further demonstrated by developing a biological growth assay in which EGF or PDGF, at concentrations as low as 1 ng/ml, stimulated SMC growth in the absence of other serum components. Under these conditions, EGF, but not PDGF, suppressed heparin inhibition as well. The ability of EGF to reverse heparin inhibition was only observed when mitogen and glycosaminoglycan were added to SMC at similar times. If SMC were pretreated with heparin for 48 hours prior to EGF addition, the protective effects of EGF were lost. Heparin did not directly prevent 125I-EGF or platelet-derived EGF-like peptides from binding to the EGF receptor on SMC. However, cultures that were pretreated with heparin for 48 hours bound 49% less 125I-EGF than cultures that had been pretreated with the mucopolysaccharide for only 2 hours or that had not been preexposed to heparin. In previous studies, we have established that heparin exerts its maximal inhibitory activity after a 48-hour treatment of SMC (Reilly et al. 1986). Taken together, these data suggest that heparin may exert its antiproliferative potential by slowly and specifically altering SMC response to EGF-like mitogens of platelet origin.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Reilly CF,Fritze LM,Rosenberg RDdoi
10.1002/jcp.1041310203subject
Has Abstractpub_date
1987-05-01 00:00:00pages
149-57issue
2eissn
0021-9541issn
1097-4652journal_volume
131pub_type
杂志文章abstract：:The smooth muscle cell population in major arteries of humans and experimental animals is heterogeneous with regard to cellular DNA content. A proportion of cells has polyploid DNA content and this proportion increases with normal aging and with hypertension. We have isolated pure populations of rat aortic smooth musc...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041250322
更新日期：1985-12-01 00:00:00
abstract：:Islets of Langerhans control whole body glucose homeostasis, as they respond, releasing hormones, to changes in nutrient concentrations in the blood stream. The regulation of hormone secretion has been the focus of attention for a long time because it is related to many metabolic disorders, including diabetes mellitus...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.25176
更新日期：2016-04-01 00:00:00
abstract：:Polyploidy has been linked to tumorigenicity mainly due to the chromosomal aberrations. Elevated reactive oxygen species (ROS) generation, on the other hand, has also been associated with oncogenic transformation in most cancer cells. However, a possible link between ploidy and ROS is largely unexplored. Here we have ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22793
更新日期：2012-02-01 00:00:00
abstract：:Glioblastoma, the most common brain tumor, is characterized by high proliferation rate, invasion, angiogenesis, and chemo- and radio-resistance. One of most remarkable feature of glioblastoma is the switch toward a glycolytic energetic metabolism that leads to high glucose uptake and consumption and a strong productio...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25421
更新日期：2017-02-01 00:00:00
abstract：:We recently reported that the growth of normal rat pleural mesothelial cells (RPMCs) is inhibited by conditioned media from either in vivo or in vitro transformed RPMCs. In this study we report that the growth of normal RPMCs is inhibited by epidermal growth factor (EGF). This was demonstrated by using three methods o...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041440118
更新日期：1990-07-01 00:00:00
abstract：:Heparan sulfate from the surface of a variety of mouse cells at different cell densities was examined by ion-exchange chromatography. The results of this analysis show that: (1) The heparan sulfate from new isolates of Swiss 3T3 cells transformed by SV40 virus (a DNA tumor virus) elutes from DEAE-cellulose at a lower ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040900108
更新日期：1977-01-01 00:00:00
abstract：:Guanine nucleotides and pertussis toxin were used to test for the involvement of a guanine nucleotide binding protein in the vasopressin V1 receptor-mediated stimulation of protein kinase C activity in Swiss 3T3 cells. Addition of vasopressin in the presence of [gamma-32P]ATP and digitonin caused a marked and rapid in...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041410204
更新日期：1989-11-01 00:00:00
abstract：:The effects of kinin B1 receptor (B1 R) deletion were examined on femur bone regeneration in streptozotocin (STZ)-type 1 diabetes. Diabetes induction in wild-type C57/BL6 (WTC57BL6) mice led to decrease in body weight and hyperglycemia, compared to the non-diabetic group of the same strain. The lack of B1 R did not af...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25034
更新日期：2015-12-01 00:00:00
abstract：:Nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) is a subtype of the NOX family, which is mainly expressed in the pulmonary vasculature and pulmonary endothelial cells in the respiratory system. NOX4 has unique characteristics, and is a constitutively active enzyme that primarily produces hydrogen peroxide...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.30005
更新日期：2021-03-01 00:00:00
abstract：:Bafilomycin A1, a specific inhibitor of H(+)-ATPases of the vacuolar type, was in the present study shown, at similar concentrations, to induce secretion of lysosomal enzyme and to elevate lysosomal pH in mouse macrophages. These results lend support to the previous suggestion of a triggering role for an increase in l...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041630116
更新日期：1995-04-01 00:00:00
abstract：:Acidic fibroblast growth factor (aFGF) mRNA was detected in a rat mammary fibroblastic cell line, but not in rat mammary epithelial cell lines or myoepithelial-like cell lines. Basic FGF (bFGF) mRNA was detected in both the fibroblasts and the myoepithelial-like cells, but was absent from the epithelial cells. A serie...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041440220
更新日期：1990-08-01 00:00:00
abstract：:The C-terminal region of parathyroid hormone-related protein (PTHrP) containing the sequence (107-111) appears to be a potent inhibitor of osteoclastic bone resorption. In the present study, we have investigated the effect of human (h)PTHrP (107-139) and hPTHrP (107-111)NH2 on the proliferation of osteoblastic rat ost...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199702)170:2<209::AID-JCP1
更新日期：1997-02-01 00:00:00
abstract：:Cell cycle-related changes in the ability to regulate cell volume following hyposmotic swelling were studied in mouse fibroblasts using videomicroscopy and the whole-cell patch clamp technique. Regulatory volume decrease (RVD) and volume-sensitive Cl- conductance (G(Cl,vol)) were measured: (1) in proliferating cells o...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/1097-4652(200104)187:1<65::AID-JCP1052>3.0
更新日期：2001-04-01 00:00:00
abstract：:The molecular mechanism by which thyroid hormones exert their effects on cell growth is still unknown. In this study, we used chick embryo hepatocytes at different stages of development as a model to investigate the effect of the two thyroid hormones, T3 and T4, and of their metabolite T2, on the control of cell proli...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20060
更新日期：2004-11-01 00:00:00
abstract：:Cell division in fertilized sea urchin eggs was reversibly inhibited when the ketoaldehyde phenyl glyoxal (PG) at a concentration of 0.1 mM was added to eggs for ten minutes prior to the formation of the mitotic spindle. We investigated whether inhibition of mitosis was due to PG binding to the cell surface (as previo...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041000119
更新日期：1979-07-01 00:00:00
abstract：:Bcl-2 associated athanogene 5 (Bag5) is a novel endoplasmic reticulum (ER) regulator. However, its role in catecholamine-induced endothelial cells damage has not been fully understood. In our study, catecholamine was used to mimic hypertension-related endothelial cell damage. Then, western blots, enzyme-linked immunos...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29904
更新日期：2021-02-01 00:00:00
abstract：:Differentiated embryo chondrocyte 1 (DEC1), a member of basic-helix-loop-helix transcription factor Bhlhe40, also called stimulated by retinoic acid 13, STRA13, plays an important role in the regulation of adipogenesis, tumorigenesis, peripheral circadian output, response to hypoxia, and development of metabolic syndr...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26471
更新日期：2018-12-01 00:00:00
abstract：BACKGROUND:Acute kidney injury (AKI) shows several kinds of disorders, which acutely harm the kidney. However, the current medical methods have limited therapeutic effects. The present study aimed to find out the molecular mechanism of AKI pathogenesis, which may provide new insights for future therapy. METHODS:Bioinf...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28118
更新日期：2019-08-01 00:00:00
abstract：:Data on the biological effects of some overexpressed oncogenes and their cooperation with cellular factors are, at least partially, contradictory. There are reports on the strong pro-apoptotic action of temperature-sensitive (ts) p53(135val) in transformed cells at permissive temperature. However, in our experience ve...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21705
更新日期：2009-05-01 00:00:00
abstract：:Non-small cell lung cancer (NSCLC) remains the leading cause of cancer death worldwide. As a platinum-based chemotherapeutic drug, cisplatin has been used in the NSCLC treatment for over 30 years, and its effects are impaired by drug resistance. This study aimed to investigate the potential role of lncRNA-AC078883.3 i...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28832
更新日期：2019-12-01 00:00:00
abstract：:These studies describe a liquid suspension culture system for normal myeloid cells derived from human foetal liver. A simple one-step fractionation procedure was employed to obtain a cell population capable of expanding into all stages of myeloid differentiation, including committed myeloid progenitor cells (GM-CFC). ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041190213
更新日期：1984-05-01 00:00:00
abstract：:During the early period after poliovirus infection of HeLa cells, cellular Na+/K+ ATPase activity is transiently activated. We investigated the possibility that Na+/K+ ATPase activation is a consequence of Na+/H+ antiporter activation. Increased uptake of the weak organic acid 5,5-dimethyloxazolidine-2,4-dione by infe...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041420319
更新日期：1990-03-01 00:00:00
abstract：:The protein tyrosine kinase pp125FAK (focal adhesion kinase, or FAK) is expressed by a variety of cell types and has been implicated in integrin-mediated signaling events. We explored the potential functions of FAK by expressing it de novo in a cell type lacking FAK. We showed previously that cultured human macrophage...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199902)178:2<164::AID-JCP5
更新日期：1999-02-01 00:00:00
abstract：:Excessive generation of mitochondrial reactive oxygen species (ROS) is considered to be initiating event in the development of diabetic nephropathy (DN). Mitochondrial biosynthesis mediated by coactivator PGC-1α and its downstream transcription factors NRF1 and TFAM may be a key target in maintaining mitochondrial fun...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27306
更新日期：2019-04-01 00:00:00
abstract：:This study was undertaken to gain more insight into the mechanism whereby TGF-beta influences the cell cycle progression of cultured rabbit articular chondrocytes. Using proliferating chondrocytes in fetal calf serum-containing medium, we have previously shown that TGF-beta induced a recruitment of cells at the end of...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041500211
更新日期：1992-02-01 00:00:00
abstract：:The next-generation sequencing (NGS) based RNA sequencing (RNA-Seq) and transcriptome profiling offers an opportunity to unveil complex biological processes. Successful RNA-Seq and transcriptome profiling requires a large amount of high-quality RNA. However, NGS-quality RNA isolation is extremely difficult from recalc...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25951
更新日期：2018-03-01 00:00:00
abstract：:Although glucocorticoids strongly affect numerous biological processes including cell growth, development, and homeostasis, their effects on migration of human mesenchymal stem cells (hMSCs) are unclear. Therefore, we investigated the role of dexamethasone (DEX) and its related signaling pathways on migration of hMSCs...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22383
更新日期：2011-03-01 00:00:00
abstract：:The activation of the Wnt/β-catenin signaling pathway is critical for skeletal development but surprisingly little is known about the requirements for the specific frizzled (Fzd) receptors that recognize Wnt ligands. To define the contributions of individual Fzd proteins to osteoblast function, we profiled the express...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29563
更新日期：2020-10-01 00:00:00
abstract：:Adiponectin, one of the adipose-derived hormone with metabolic activity, has been reported to conversely affect angiogenesis of endothelial cells in vitro. The previous study in animal models has demonstrated that adiponectin has a protective role in retinal vascular injury following pathological stimuli. However, cli...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28659
更新日期：2019-11-01 00:00:00
abstract：:A variant of the hypoxanthine-guanine phosphoribosyltransferase deficient, and adenine phosphoribosyltransferase deficient mouse resistant to 6-azauridine. These cells are not only resistant to 6-azauridine (5 X 10(-4) M), but also to adenosine (10(-3) M). Resistance persists indefinitely even in the absence of both c...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040860202
更新日期：1975-10-01 00:00:00
