A calcium requirement for movement of cultured cells.


:When calcium is removed from culture medium, motility of cultured cells is decreased. The effect is rapid, reversible and pronounced. Decreased motility is observed with normal mouse Balb/c 3T3 cells, mouse L929 cells, rat kidney fibroblasts and chick embryo fibroblasts. The calcium dependence of movement can be observed both with individual cells and with the movement of the margin of a monolayer into a wound. Magnesium will not substitute for calcium to maintain motility. Strontium will substitute, but is not as effective as calcium for maintaining cell movement. Low concentrations of the divalent cation ionophore A23187 (0.5-1 micron) partially reverse the reduced migration observed at low calcium concentrations. These results are consistent with the hypothesis that movement of non-muscle cells occurs through mechanisms similar to those important in the contraction of muscle.


J Cell Physiol


Moore L,Pastan I





Has Abstract


1979-10-01 00:00:00












  • Recombinant non-collagenous domain of alpha2(IV) collagen causes involution of choroidal neovascularization by inducing apoptosis.

    abstract::Vascular endothelial cells receive proangiogenic or antiangiogenic signals from components of extracellular matrix (ECM) depending upon the situation and many molecular signals can have opposite effects in different vascular beds. Tissue inhibitor of metalloproteinase 1 is antiangiogenic in several tissues, but promot...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lima E Silva R,Kachi S,Akiyama H,Shen J,Aslam S,Yuan Gong Y,Khu NH,Hatara MC,Boutaud A,Peterson R,Campochiaro PA

    更新日期:2006-07-01 00:00:00

  • Transfer of functional EGF receptors to an IL3-dependent cell line.

    abstract::Epidermal growth factor (EGF) is a small protein that acts as a mitogen for various epidermal, epithelial, and fibroblastic cells that bear specific EGF receptors. The molecule that binds EGF is a 175-kD transmembrane protein, with an extracellular ligand binding domain and an intracellular domain that possesses tyros...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Collins MK,Downward J,Miyajima A,Maruyama K,Arai K,Mulligan RC

    更新日期:1988-11-01 00:00:00

  • Relationship between c-Kit expression and proliferation in acute myeloblastic leukemia cell lines.

    abstract::We have proposed that the transmembrane receptor encoded by the c-Kit protooncogene and its ligand play an important role in regulating the proliferation of blasts cells in acute myeloblastic leukemia (AML). To test this hypothesis, immunobeads were used to separate blasts from three Kit-expression positive cell lines...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tohda S,Yang GS,Ashman LK,McCulloch EA,Minden MD

    更新日期:1993-02-01 00:00:00

  • Identification of a prognostic alternative splicing signature in oral squamous cell carcinoma.

    abstract::Alternative splicing (AS) is critically associated with tumorigenesis and patient's prognosis. Here, we systematically analyzed survival-associated AS signatures in oral squamous cell carcinoma (OSCC) and evaluated their prognostic predictive values. Survival-related AS events were identified by univariate and multiva...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhang S,Wu X,Diao P,Wang C,Wang D,Li S,Wang Y,Cheng J

    更新日期:2020-05-01 00:00:00

  • Uptake of [3H]ouabain from the cell surface into the lysosomal compartment of HeLa cells.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Cook JS,Tate EH,Shaffer C

    更新日期:1982-01-01 00:00:00

  • Down-modulation of EGF receptors in cells transformed by the src oncogene.

    abstract::The effects of src oncogene expression on epidermal growth factor (EGF) receptors have been investigated in mouse 3T3 and rat-1 fibroblasts. Transformation of both cell types with src resulted in marked reductions in cellular EGF receptor levels, as assayed by either 125I-EGF binding or immunoprecipitation of receptor...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wasilenko WJ,Shawver LK,Weber MJ

    更新日期:1987-06-01 00:00:00

  • Functional discontinuities in prothymosin alpha caused by caspase cleavage in apoptotic cells.

    abstract::Our study examines the effect of apoptosis on prothymosin alpha, an abundant, nuclear protein intimately involved with proliferation of all mammalian cells. When HeLa cells were treated with actinomycin D, with etoposide, or with staurosporine following synchronization with hydroxyurea, they underwent apoptosis based ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Enkemann SA,Wang RH,Trumbore MW,Berger SL

    更新日期:2000-02-01 00:00:00

  • Proliferation of rat small hepatocytes requires follistatin expression.

    abstract::Small hepatocytes (SHs) are a subpopulation of hepatocytes that have high growth potential in culture and can differentiate into mature hepatocytes (MHs). The activin (Act)/follistatin (Fst) system critically contributes to homeostasis of cell growth in the normal liver. ActA and ActB consist of two disulfide-linked I...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ooe H,Chen Q,Kon J,Sasaki K,Miyoshi H,Ichinohe N,Tanimizu N,Mitaka T

    更新日期:2012-06-01 00:00:00

  • p38SJ, a novel DINGG protein protects neuronal cells from alcohol induced injury and death.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Amini S,Merabova N,Khalili K,Darbinian N

    更新日期:2009-12-01 00:00:00

  • The adhesive glycoprotein laminin is an agglutinin.

    abstract::The glycoprotein laminin appears to function in the attachment of various epithelial cells to basement membranes. We examined whether its putative cell-adhesive activity could be analyzed in a simple, one-component model system--the agglutination of erythrocytes. Laminin is a potent agglutinin of aldehyde-fixed sheep ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kennedy DW,Rohrbach DH,Martin GR,Momoi T,Yamada KM

    更新日期:1983-03-01 00:00:00

  • Cell cycle changes in transformed cells growing under serum-free conditions.

    abstract::Polyoma transformed hamster cells (PyBHK) and SV40 transformed mouse cells (SV3T3) were transferred in culture using crystalline trypsin followed by neutralisation with soybean trypsin inhibitor. Such cells were able to proliferate freely in defined medium without any serum supplement and without any intervening perio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bush H,Shodell M

    更新日期:1977-03-01 00:00:00

  • Circulating and tissue resident endothelial progenitor cells.

    abstract::Progenitor cells for the endothelial lineage have been widely investigated for more than a decade, but continue to be controversial since no unique identifying marker has yet been identified. This review will begin with a discussion of the basic tenets originally proposed for proof that a cell displays properties of a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Basile DP,Yoder MC

    更新日期:2014-01-01 00:00:00

  • Requirement for PLCγ2 in IL-3 and GM-CSF-stimulated MEK/ERK phosphorylation in murine and human hematopoietic stem/progenitor cells.

    abstract::Even though the involvement of intracellular Ca(2+) Ca(i)(2+) in hematopoiesis has been previously demonstrated, the relationship between Ca(i)(2+) signaling and cytokine-induced intracellular pathways remains poorly understood. Herein, the molecular mechanisms integrating Ca(2+) signaling with the extracellular signa...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Leon CM,Barbosa CM,Justo GZ,Borelli P,Resende JD Jr,de Oliveira JS,Ferreira AT,Paredes-Gamero EJ

    更新日期:2011-07-01 00:00:00

  • TGF-beta-induced G2/M delay in proliferating rabbit articular chondrocytes is associated with an enhancement of replication rate and a cAMP decrease: possible involvement of pertussis toxin-sensitive pathway.

    abstract::This study was undertaken to gain more insight into the mechanism whereby TGF-beta influences the cell cycle progression of cultured rabbit articular chondrocytes. Using proliferating chondrocytes in fetal calf serum-containing medium, we have previously shown that TGF-beta induced a recruitment of cells at the end of...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Vivien D,Galéra P,Lebrun E,Daireaux M,Loyau G,Pujol JP

    更新日期:1992-02-01 00:00:00

  • Generation of insulin-producing hepatocyte-like cells from human Wharton's jelly mesenchymal stem cells as an alternative source of islet cells.

    abstract::Islet cell transplantation, as a treatment of type 1 diabetes, has a lot of complexity such as allograft rejections and an insufficient number of donors. The liver can be used as a replacement for endogenous insulin production. Hepatocytes can inherently respond to glucose levels and secrete proteins. Utilization of m...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Beikmohammadi L,Bandehpour M,Hashemi SM,Kazemi B

    更新日期:2019-08-01 00:00:00

  • Kidney proximal tubular cells isolated by collagenase perfusion grow in defined media in the absence of growth factors.

    abstract::Cells from kidney proximal tubules have been successfully isolated, characterized, and cultured from male Fischer 344 rats between 150-400 g using a two-step collagenase perfusion. The cells undergo high levels of DNA synthesis and mitosis in both serum free media (with an without hormone supplementation) and media co...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Rosenberg MR,Michalopoulos G

    更新日期:1987-04-01 00:00:00

  • DNA methylation and cancer.

    abstract::The methylation of DNA is an epigenetic modification that can play an important role in the control of gene expression in mammalian cells. The enzyme involved in this process is DNA methyltransferase, which catalyzes the transfer of a methyl group from S-adenosyl-methionine to cytosine residues to form 5-methylcytosin...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Momparler RL,Bovenzi V

    更新日期:2000-05-01 00:00:00

  • Downregulation of uPAR promotes urokinase translocation into the nucleus and epithelial to mesenchymal transition in neuroblastoma.

    abstract::The urokinase system is involved in a variety of physiological processes, such as fibrinolysis, matrix remodeling, wound healing, and regeneration. Upon binding to its cognate receptor urokinase-type plasminogen activator receptor (uPAR), urokinase-type plasminogen activator (uPA) catalyzes the conversion of plasminog...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Semina EV,Rubina KA,Shmakova AA,Rysenkova KD,Klimovich PS,Aleksanrushkina NA,Sysoeva VY,Karagyaur MN,Tkachuk VA

    更新日期:2020-09-01 00:00:00

  • Establishment and characterization of a telomerase-immortalized porcine bronchial epithelial cell line.

    abstract::Primary porcine bronchial epithelial cells (PBECs) are an ideal model to study the molecular and pathogenic mechanisms of various porcine respiratory pathogens. However, the short lifespan of primary PBECs greatly limit their application. Here, we isolated and cultured primary PBECs and established immortalized PBECs ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Xie X,Gan Y,Pang M,Shao G,Zhang L,Liu B,Xu Q,Wang H,Feng Y,Yu Y,Chen R,Wu M,Zhang Z,Hua L,Xiong Q,Liu M,Feng Z

    更新日期:2018-12-01 00:00:00

  • Peptidoglycan induces interleukin-6 expression through the TLR2 receptor, JNK, c-Jun, and AP-1 pathways in microglia.

    abstract::We recently reported that peptidoglycan (PGN), a cell wall component of the Gram-positive bacterium, induces NF-κB activation and microglia activation. However, PGN-regulated AP-1 activation and cytokine expression in microglia remains unclear. This study investigated how PGN influences the signaling pathway involved ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lin HY,Tang CH,Chen JH,Chuang JY,Huang SM,Tan TW,Lai CH,Lu DY

    更新日期:2011-06-01 00:00:00

  • Annexin A1: novel roles in skeletal muscle biology.

    abstract::Annexin A1 (ANXA1, lipocortin-1) is the first characterized member of the annexin superfamily of proteins, so called since their main property is to bind (i.e., to annex) to cellular membranes in a Ca(2+) -dependent manner. ANXA1 has been involved in a broad range of molecular and cellular processes, including anti-in...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Bizzarro V,Petrella A,Parente L

    更新日期:2012-08-01 00:00:00

  • Timing of protooncogene expression varies in toxin-induced liver regeneration.

    abstract::Hepatic expression of the protooncogenes c-fos and c-myc occurs within 2 h after partial hepatectomy, and these immediate early genes are thought to prime the hepatocytes for subsequent proliferation. To examine whether such gene activation occurred in the setting of hepatocyte proliferation after toxic liver injury, ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Schmiedeberg P,Biempica L,Czaja MJ

    更新日期:1993-02-01 00:00:00

  • Regulation of cellular growth by sodium pump activity.

    abstract::Cellular growth has been found to be directly related to the amount of sodium pumping activity in mouse lymphoblasts (L5178-Y) cultured in varying concentrations of the cardiac glycoside, ouabain. No short-term adaptation (within one generation) occured; i.e., neither growth rate nor (Na+ + K+)-ATPase activity increas...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Shank BB,Smith NE

    更新日期:1976-03-01 00:00:00

  • Increased transcriptional response to mechanical strain in keloid fibroblasts due to increased focal adhesion complex formation.

    abstract::Clinicians have observed that keloids preferentially form in body areas subject to increased skin tension. We hypothesized a difference exists in the transcriptional response of keloid fibroblasts to mechanical strain compared with normal fibroblasts. Normal and keloid fibroblasts were seeded in a device calibrated to...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wang Z,Fong KD,Phan TT,Lim IJ,Longaker MT,Yang GP

    更新日期:2006-02-01 00:00:00

  • Aluminum fluoride stimulates inositol phosphate metabolism and inhibits expression of differentiation markers in mouse keratinocytes.

    abstract::Mouse keratinocytes are induced to differentiate in vitro by elevating the level of extracellular calcium from 0.05 mM, where keratinocytes express a basal cell phenotype, to greater than 0.10 mM, where they express the differentiated phenotype. This process has been associated with a rapid, sustained increase in inos...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lee E,Yuspa SH

    更新日期:1991-07-01 00:00:00

  • Syndecans in chronic inflammatory and autoimmune diseases: Pathological insights and therapeutic opportunities.

    abstract::Syndecans (SDCs) are a family of heparan sulfate proteoglycans (HSPGs) glycoproteins ubiquitously expressed on the cell surfaces and extracellular matrix of all mammalian tissues. There are four mammalian syndecans, SDC-1 thorough 4, which play a critical role in cell adhesion, migration, proliferation, differentiatio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Agere SA,Kim EY,Akhtar N,Ahmed S

    更新日期:2018-09-01 00:00:00

  • Calcium transport by sarcoplasmic reticulum of vascular smooth muscle: II. Effects of calmodulin and calmodulin inhibitors.

    abstract::The role of calmodulin (CaM) in modulating calcium (Ca) uptake by sarcoplasmic reticulum (SR) of vascular smooth muscle was studied in saponin skinned strips of rat caudal artery. Exogenous CaM concentrations ranging from 0.3-1.8 microM did not statistically change the steady state MgATP-dependent Ca content, the MgAT...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Stout MA,Silver PJ

    更新日期:1992-10-01 00:00:00

  • Role of insulin-like growth factor binding protein-3 (IGFBP-3) in the differentiation of primary human adult skeletal myoblasts.

    abstract::Although muscle satellite cells were identified almost 40 years ago, little is known about the induction of their proliferation and differentiation in response to physiological/pathological stimuli or to growth factors/cytokines. In order to investigate the role of the insulin-like growth factor (IGF)/IGF binding prot...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Foulstone EJ,Savage PB,Crown AL,Holly JM,Stewart CE

    更新日期:2003-04-01 00:00:00

  • The non-genomic effects on Na+/H+-exchange 1 by progesterone and 20alpha-hydroxyprogesterone in human T cells.

    abstract::Progesterone is an endogenous immunomodulator and can suppress T-cell activation during pregnancy. We have previously shown that the non-genomic effects of progesterone, especially acidification, are exerted via plasma membrane sites and suppress cellular genomic responses to mitogens. This study aimed to show that ac...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chien EJ,Liao CF,Chang CP,Pu HF,Lu LM,Shie MC,Hsieh DJ,Hsu MT

    更新日期:2007-05-01 00:00:00

  • Cyanidin Chloride inhibits ovariectomy-induced osteoporosis by suppressing RANKL-mediated osteoclastogenesis and associated signaling pathways.

    abstract::Over-production and activation of osteoclasts is a common feature of osteolytic conditions such as osteoporosis, tumor-associated osteolysis, and inflammatory bone erosion. Cyanidin Chloride, a subclass of anthocyanin, displays antioxidant and anti-carcinogenesis properties, but its role in osteoclastic bone resorptio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Cheng J,Zhou L,Liu Q,Tickner J,Tan Z,Li X,Liu M,Lin X,Wang T,Pavlos NJ,Zhao J,Xu J

    更新日期:2018-03-01 00:00:00