Modulation of the in vitro angiogenic potential of human mesenchymal stromal cells from different tissue sources.

Abstract:

:Mesenchymal stromal cells (MSCs) have been widely exploited for the treatment of several conditions due to their intrinsic regenerative and immunomodulatory properties. MSC have demonstrated to be particularly relevant for the treatment of ischemic diseases, where MSC-based therapies can stimulate angiogenesis and induce tissue regeneration. Regardless of the condition targeted, recent analyses of MSC-based clinical trials have demonstrated limited benefits indicating a need to improve the efficacy of this cell product. Preconditioning MSC ex vivo through microenvironment modulation was found to improve MSC survival rate and thus prolong their therapeutic effect. This workstudy aims at enhancing the in vitro angiogenic capacity of a potential MSC-based medicinal product by comparing different sources of MSC and culture conditions. MSC from three different sources (bone marrow [BM], adipose tissue [AT], and umbilical cord matrix [UCM]) were cultured with xenogeneic-/serum-free culture medium under static conditions and their angiogenic potential was studied. Results indicated a higher in vitro angiogenic capacity of UCM MSC, compared with cells derived from BM and AT. Physicochemical preconditioning of UCM MSC through a microcarrier-based culture platform and low oxygen concentration (2% O2 , compared with atmospheric air) increased the in vitro angiogenic potential of the cultured cells. Envisaging the clinical manufacturing of an allogeneic, off-the-shelf MSC-based product, preconditioned UCM MSC maintain the angiogenic gene expression profile upon cryopreservation and delivery processes in the conditions of our study. These results are expected to contribute to the development of MSC-based therapies in the context of angiogenesis.

journal_name

J Cell Physiol

authors

Pinto DS,Ahsan T,Serra J,Fernandes-Platzgummer A,Cabral JMS,da Silva CL

doi

10.1002/jcp.29622

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

7224-7238

issue

10

eissn

0021-9541

issn

1097-4652

journal_volume

235

pub_type

杂志文章
  • Effects of endothelins on hepatic stellate cell synthesis of endothelin-1 during hepatic wound healing.

    abstract::Endothelin-1 production is increased after liver injury and the subsequent wounding response. Further, endothelin-1 has prominent effects on hepatic stellate cells (key effectors of the hepatic wounding response), including on collagen synthesis, proliferation, and expression of smooth muscle proteins. We tested the h...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.10110

    authors: Shao R,Rockey DC

    更新日期:2002-06-01 00:00:00

  • Combination of low O(2) concentration and mesenchymal stromal cells during culture of cord blood CD34(+) cells improves the maintenance and proliferative capacity of hematopoietic stem cells.

    abstract::The physiological approach suggests that an environment associating the mesenchymal stromal cells (MSC) and low O(2) concentration would be most favorable for the maintenance of hematopoietic stem cells (HSCs) in course of ex vivo expansion of hematopoietic grafts. To test this hypothesis, we performed a co-culture of...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.23019

    authors: Hammoud M,Vlaski M,Duchez P,Chevaleyre J,Lafarge X,Boiron JM,Praloran V,Brunet De La Grange P,Ivanovic Z

    更新日期:2012-06-01 00:00:00

  • Binding and phagocytosis of fibronectin-coated beads by BHK cells: receptor specificity and dynamics.

    abstract::Studies on the receptor specificity and dynamics involved in fibroblast phagocytosis of latex beads revealed the following: 1) Ligands other than fibronectin such as concanavalin A (ConA) and serum spreading factor, when coated on latex beads, were found to promote phagocytosis of the beads. This indicates that fibrob...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041240211

    authors: McAbee DD,Grinnell F

    更新日期:1985-08-01 00:00:00

  • IL-37-induced activation of glycogen synthase kinase 3β promotes IL-1R8/Sigirr phosphorylation, internalization, and degradation in lung epithelial cells.

    abstract::Interleukin (IL)-37 diminishes a variety of inflammatory responses through ligation to its receptor IL-1R8/Sigirr. Sigirr is a Toll like receptor/IL-1R family member. We have shown that Sigirr is not stable in response to IL-37 treatment. IL-37-induced Sigirr degradation is mediated by the ubiquitin-proteasome system,...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.30253

    authors: Li L,Wei J,Suber TL,Ye Q,Miao J,Li S,Taleb SJ,Tran KC,Tamaskar AS,Zhao J,Zhao Y

    更新日期:2021-01-05 00:00:00

  • Bone production by human maxillary sinus mucosa cells.

    abstract::The Schneider membrane is the mucosa that covers the inner part of the maxillary sinus cavities. The free surface is a ciliated pseudostratified epithelium, while the deeper portion is a highly vascularized connective tissue. The stromal fraction, bordering the bony wall of the sinus, after tooth loss can exhibit incr...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24022

    authors: Graziano A,Benedetti L,Massei G,Cusella de Angelis MG,Ferrarotti F,Aimetti M

    更新日期:2012-09-01 00:00:00

  • Forskolin has biphasic effects on osteoprogenitor cell differentiation in vitro.

    abstract::Cells isolated from fetal rat calvaria (RC) and maintained in vitro in medium containing ascorbic acid and B-glycerophosphate form three-dimensional, mineralized nodules having the histological, immunohistological, and ultrastructural characteristics of woven bone. We have studied the effects of forskolin (FSK), a dit...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041420109

    authors: Turksen K,Grigoriadis AE,Heersche JN,Aubin JE

    更新日期:1990-01-01 00:00:00

  • Carbonic anhydrase cytochemistry in mitochondria-rich cells of salamander larvae gill epithelium as related to age and H+ and Na+ concentrations.

    abstract::Carbonic anhydrase (CAH) was localized in the mitochondria-rich cells (MRC) of 1-week-old salamander larvae gill epithelium, in both MRC and pavement cells of 6-week-old larvae, and in regenerated stems of previously amputated gills. CAH activity of the MRC was measured quantitatively using a microscope densitometric ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041300118

    authors: Lewinson D,Rosenberg M,Goldenberg S,Warburg MR

    更新日期:1987-01-01 00:00:00

  • Melatonin-mediated regulation of autophagy: Making sense of double-edged sword in cancer.

    abstract::Much research has been conducted to discover novel techniques to reverse the process of tumorigenesis and, cure already stablished malignancies. One well-stablished approach has been the use of organic compounds and naturally found agents such as melatonin whose anticancer effects have been shown in multiple studies, ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.28435

    authors: Mirza-Aghazadeh-Attari M,Mohammadzadeh A,Adib A,Darband SG,Sadighparvar S,Mihanfar A,Majidinia M,Yousefi B

    更新日期:2019-08-01 00:00:00

  • In Vitro and In Vivo Differentiation of Progenitor Stem Cells Obtained After Mechanical Digestion of Human Dental Pulp.

    abstract::Human population is facing a revolutionary change in the demographic structure with an increasing number of elderly people requiring an unmet need to ensure a smooth aging process and dental care is certainly an important aspect that has to be considered. To date, dentistry has been conservative and the need of transf...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25452

    authors: Monti M,Graziano A,Rizzo S,Perotti C,Del Fante C,d'Aquino R,Redi CA,Rodriguez Y Baena R

    更新日期:2017-03-01 00:00:00

  • Cleavage of the epidermal growth factor receptor by a membrane-bound leupeptin-sensitive protease active in nonionic detergent lysates of senescent but not young human diploid fibroblasts.

    abstract::Numerous studies suggest that epidermal growth factor (EGF) signaling is impaired in nonproliferating senescent human diploid fibroblasts downstream of receptor binding. One possible explanation for these results is that senescent cells possess unique enzymatic activities capable of regulating functional levels of the...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041600305

    authors: Carlin C,Phillips PD,Brooks-Frederich K,Knowles BB,Cristofalo VJ

    更新日期:1994-09-01 00:00:00

  • A new insight into thymosin β4, a promising therapeutic approach for neurodegenerative disorders.

    abstract::Thymosin β4 (Tβ4), a G-actin-sequestering secreted peptide, improves neurovascular remodeling and central nervous system plasticity, which leads to neurological recovery in many neurological diseases. Inflammatory response adjustment and tissue inflammation consequences from neurological injury are vital for neurologi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.29293

    authors: Shomali N,Baradaran B,Deljavanghodrati M,Akbari M,Hemmatzadeh M,Mohammadi H,Jang Y,Xu H,Sandoghchian Shotorbani S

    更新日期:2020-04-01 00:00:00

  • Delay of M-phase onset by aphidicolin can retain the nuclear localization of zinc and metallothionein in 3T3-L1 fibroblasts.

    abstract::The transient nuclear localization of metallothionein during cell growth and differentiation may be related to the increased requirement of zinc for DNA synthesis, activation of metalloenzymes, and transcription factors. Treatment of 3T3-L1 fibroblasts with aphidicolin, an inhibitor of nuclear DNA synthesis, caused a ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(200005)183:2<247::AID-JCP1

    authors: Apostolova MD,Cherian MG

    更新日期:2000-05-01 00:00:00

  • Cytomatrix synthesis in MDCK epithelial cells.

    abstract::Detailed information regarding the synthesis rates of individual protein components is important in understanding the assembly and dynamics of the cytoskeletal matrix of eukaryotic cells. As an approach to this topic, the dual isotope technique of Clark and Zak (J. Biol. Chem., 256:4863-4870, 1981), was employed to me...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041430315

    authors: Mitchell JJ,Low RB,Woodcock-Mitchell JL

    更新日期:1990-06-01 00:00:00

  • Molecular characterization of a human cation-Cl- cotransporter (SLC12A8A, CCC9A) that promotes polyamine and amino acid transport.

    abstract::Cation-Cl- cotransporters (CCCs) belong to a large family of proteins that includes 9 isoforms, two of which have still not been ascribed a transport function (CCC8 and CCC9) while the others are all known to promote Cl(-)-coupled Na+ and/or K+ movement at the cell surface. The CCCs are also included in a larger famil...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21814

    authors: Daigle ND,Carpentier GA,Frenette-Cotton R,Simard MG,Lefoll MH,Noël M,Caron L,Noël J,Isenring P

    更新日期:2009-09-01 00:00:00

  • Oxidative stress induces GLUT4 translocation by activation of PI3-K/Akt and dual AMPK kinase in cardiac myocytes.

    abstract::In response to metabolic stress, GLUT4, the most abundant glucose transporter, translocates from intracellular vesicles to the plasma membrane. This appears to play an important role in protecting cardiac myocytes from ischemic injury. To investigate the precise mechanisms of GLUT4 translocation in cardiomyocytes, we ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21353

    authors: Horie T,Ono K,Nagao K,Nishi H,Kinoshita M,Kawamura T,Wada H,Shimatsu A,Kita T,Hasegawa K

    更新日期:2008-06-01 00:00:00

  • Effect of substrata and fibroblast growth factor on the proliferation in vitro of bovine aortic endothelial cells.

    abstract::The hypothesis that, in the case of clonal or low-density cultures, cells which do not readily proliferate are those that do not produce an extracellular matrix (ECM), while those that proliferate actively are cells that have retained their ability to produce it, has been tested using low-density vascular endothelial ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041090109

    authors: Gospodarowicz D,Lui GM

    更新日期:1981-10-01 00:00:00

  • Effect of polyunsaturated fatty acids and phospholipids on [3H]-vitamin E incorporation into pulmonary artery endothelial cell membranes.

    abstract::Vitamin E, a dietary antioxidant, is presumed to be incorporated into the lipid bilayer of biological membranes to an extent proportional to the amount of polyunsaturated fatty acids or phospholipids in the membrane. In the present study we evaluated the distribution of incorporated polyunsaturated fatty acids (PUFA) ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041450324

    authors: Sekharam KM,Patel JM,Block ER

    更新日期:1990-12-01 00:00:00

  • Extracellular acidification enhances DNA binding activity of MafG-FosB heterodimer.

    abstract::Cells are quite sensitive to a change of the extracellular pH and respond to it through detection of the H+/HCO3- level in extracellular fluid. However, little is known about molecular details induced by acidosis, such as intracellular pathways and gene expression. Here we describe properties of gene expression, prote...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20369

    authors: Shimokawa N,Kumaki I,Qiu CH,Ohmiya Y,Takayama K,Koibuchi N

    更新日期:2005-10-01 00:00:00

  • Evidence for the participation of nerve growth factor and its low-affinity receptor (p75NTR) in the regulation of the myogenic program.

    abstract::We have studied expression and function of neurotrophins and their receptors during myogenic differentiation of C2C12 cells, a clonal cell line derived from mouse muscle that is capable of in vitro differentiation. The genes coding for nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and their commo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199807)176:1<10::AID-JCP2>

    authors: Seidl K,Erck C,Buchberger A

    更新日期:1998-07-01 00:00:00

  • A P53-related microRNA model for predicting the prognosis of hepatocellular carcinoma patients.

    abstract::Studies have shown that microRNAs (miRNAs) play a vital role in tumor progression and patients' prognosis. Therefore, we aimed to construct a miRNA model for forecasting the survival of hepatocellular carcinoma (HCC) patients. The gene expression data of 433 patients with HCC from The Cancer Genome Atlas (TCGA) and Ge...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29245

    authors: Fang SS,Guo JC,Zhang JH,Liu JN,Hong S,Yu B,Gao Y,Hu SP,Liu HZ,Sun L,Zhao Y

    更新日期:2020-04-01 00:00:00

  • Annexin A1: novel roles in skeletal muscle biology.

    abstract::Annexin A1 (ANXA1, lipocortin-1) is the first characterized member of the annexin superfamily of proteins, so called since their main property is to bind (i.e., to annex) to cellular membranes in a Ca(2+) -dependent manner. ANXA1 has been involved in a broad range of molecular and cellular processes, including anti-in...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.24032

    authors: Bizzarro V,Petrella A,Parente L

    更新日期:2012-08-01 00:00:00

  • MiR-139 promotes differentiation of bovine skeletal muscle-derived satellite cells by regulating DHFR gene expression.

    abstract::MicroRNAs play an important regulatory role in the proliferation and differentiation of skeletal muscle-derived satellite cells (MDSCs). In particular, miR-139 can inhibit tumor cell proliferation and invasion, and its expression is down-regulated during C2C12 myoblast differentiation. The aim of this study was thus t...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26817

    authors: Zhou S,Li S,Zhang W,Tong H,Li S,Yan Y

    更新日期:2018-01-01 00:00:00

  • Tfcp2l1 safeguards the maintenance of human embryonic stem cell self-renewal.

    abstract::Tfcp2l1 is a transcription factor critical for mouse embryonic stem cell (mESC) maintenance. However, its role in human ESCs (hESCs) remains unclear. Here, we investigated the role of Tfcp2l1 in controlling hESC activity and showed that Tfcp2l1 is functionally important in the maintenance of hESC identity. Tfcp2l1 exp...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26483

    authors: Sun H,You Y,Guo M,Wang X,Zhang Y,Ye S

    更新日期:2018-09-01 00:00:00

  • Down-modulation of EGF receptors in cells transformed by the src oncogene.

    abstract::The effects of src oncogene expression on epidermal growth factor (EGF) receptors have been investigated in mouse 3T3 and rat-1 fibroblasts. Transformation of both cell types with src resulted in marked reductions in cellular EGF receptor levels, as assayed by either 125I-EGF binding or immunoprecipitation of receptor...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041310318

    authors: Wasilenko WJ,Shawver LK,Weber MJ

    更新日期:1987-06-01 00:00:00

  • Is there a glycosaminoglycan-related heterogeneity of the thymic epithelium?

    abstract::We determined the synthesis and secretion of glycosaminoglycans by three distinct preparations of mouse cultured thymic epithelial cells. These comprised primary cultures of thymic nurse cells (TNCs), which are normally located within the cortex of the thymic lobules, as well as two murine thymic epithelial cells, bea...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/1097-4652(200010)185:1<68::AID-JCP6>3.0.CO

    authors: Werneck CC,Cruz MS,Silva LC,Villa-Verde DM,Savino W,Mourão PA

    更新日期:2000-10-01 00:00:00

  • Enhanced transbilayer mobility of phospholipids in malaria-infected monkey erythrocytes: a spin-label study.

    abstract::Using a recently described technique of electron spin resonance spectroscopy, we determined the rate of transbilayer mobility (flip) of the four major simian-erythrocyte phospholipids in the erythrocyte membrane after infection by Plasmodium knowlesi. The development of the malarial parasite induces a very large incre...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041350113

    authors: Beaumelle BD,Vial HJ,Bienvenüe A

    更新日期:1988-04-01 00:00:00

  • Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPase(old-35)).

    abstract::As a strategy to identify gene expression changes affected by human polynucleotide phosphorylase (hPNPase(old-35)), we performed gene expression analysis of HeLa cells in which hPNPase(old-35) was overexpressed. The observed changes were then compared to those of HO-1 melanoma cells in which hPNPase(old-35) was stably...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24645

    authors: Sokhi UK,Bacolod MD,Emdad L,Das SK,Dumur CI,Miles MF,Sarkar D,Fisher PB

    更新日期:2014-12-01 00:00:00

  • Effects of interaction between estradiol-17 beta and progesterone on the proliferation of cloned breast tumor cells (MCF-7 and T47D).

    abstract::We have previously reported that the proliferation of cloned MCF-7 and T47D human mammary tumor cells can be inhibited by increasing concentrations of charcoal-dextran stripped female human serum (CDFHS). The maximal proliferation rate was restored by the addition of 3 X 10(-11) M estradiol-17 beta to the culture medi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041240305

    authors: Schatz RW,Soto AM,Sonnenschein C

    更新日期:1985-09-01 00:00:00

  • Inhibition of human methionine adenosyltransferase 1A transcription by coding region methylation.

    abstract::Two genes (MAT1A and MAT2A) encode for the essential enzyme methionine adenosyltransferase (MAT). MAT1A is silenced in hepatocellular carcinoma (HCC), and absence of MAT1A leads to spontaneous development of HCC in mice. Previous report correlated promoter methylation to silencing of MAT1A but definitive proof was lac...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22875

    authors: Tomasi ML,Li TW,Li M,Mato JM,Lu SC

    更新日期:2012-04-01 00:00:00

  • Control of melanin synthesis and secretion by B16/C3 melanoma cells.

    abstract::In culture, B16/C3 murine melanoma cells grown in the presence of serum undergo melanogenesis at a specific time after plating. At this time, melanin is synthesized intracellularly and then secreted into the extracellular culture fluid. We have found that melanin secretion is dependent on the presence of serum in the ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041130318

    authors: Laskin JD,Piccinini L,Engelhardt DL,Weinstein IB

    更新日期:1982-12-01 00:00:00