Modulation of the in vitro angiogenic potential of human mesenchymal stromal cells from different tissue sources.


:Mesenchymal stromal cells (MSCs) have been widely exploited for the treatment of several conditions due to their intrinsic regenerative and immunomodulatory properties. MSC have demonstrated to be particularly relevant for the treatment of ischemic diseases, where MSC-based therapies can stimulate angiogenesis and induce tissue regeneration. Regardless of the condition targeted, recent analyses of MSC-based clinical trials have demonstrated limited benefits indicating a need to improve the efficacy of this cell product. Preconditioning MSC ex vivo through microenvironment modulation was found to improve MSC survival rate and thus prolong their therapeutic effect. This workstudy aims at enhancing the in vitro angiogenic capacity of a potential MSC-based medicinal product by comparing different sources of MSC and culture conditions. MSC from three different sources (bone marrow [BM], adipose tissue [AT], and umbilical cord matrix [UCM]) were cultured with xenogeneic-/serum-free culture medium under static conditions and their angiogenic potential was studied. Results indicated a higher in vitro angiogenic capacity of UCM MSC, compared with cells derived from BM and AT. Physicochemical preconditioning of UCM MSC through a microcarrier-based culture platform and low oxygen concentration (2% O2 , compared with atmospheric air) increased the in vitro angiogenic potential of the cultured cells. Envisaging the clinical manufacturing of an allogeneic, off-the-shelf MSC-based product, preconditioned UCM MSC maintain the angiogenic gene expression profile upon cryopreservation and delivery processes in the conditions of our study. These results are expected to contribute to the development of MSC-based therapies in the context of angiogenesis.


J Cell Physiol


Pinto DS,Ahsan T,Serra J,Fernandes-Platzgummer A,Cabral JMS,da Silva CL




Has Abstract


2020-10-01 00:00:00












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    pub_type: 杂志文章


    authors: Eguchi R,Fujimori Y,Takeda H,Tabata C,Ohta T,Kuribayashi K,Fukuoka K,Nakano T

    更新日期:2011-03-01 00:00:00

  • Low oxygen tension increases mRNA levels of alpha 1 (I) procollagen in human dermal fibroblasts.

    abstract::Dermal fibroblasts exposed to low oxygen tension show upregulated synthesis of transforming growth factor-beta 1 (TGF-beta 1), an established stimulatory peptide in the formation of extracellular matrix proteins. In this report, procollagen synthesis was measured in cultures of confluent adult human dermal fibroblasts...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Falanga V,Martin TA,Takagi H,Kirsner RS,Helfman T,Pardes J,Ochoa MS

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  • SIRT6 deacetylase transcriptionally regulates glucose metabolism in heart.

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    journal_title:Journal of cellular physiology

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    authors: Khan D,Sarikhani M,Dasgupta S,Maniyadath B,Pandit AS,Mishra S,Ahamed F,Dubey A,Fathma N,Atreya HS,Kolthur-Seetharam U,Sundaresan NR

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  • The role of p63 in germ cell apoptosis in the developing testis.

    abstract::The fetal and neonatal development of male germ cells (gonocytes) is a poorly understood but crucial process for establishing fertility. In rodents, gonocytes go through two phases of proliferation accompanied by apoptosis and separated by a quiescent period during the end of fetal development. P63 is a member of the ...

    journal_title:Journal of cellular physiology

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    authors: Petre-Lazar B,Livera G,Moreno SG,Trautmann E,Duquenne C,Hanoux V,Habert R,Coffigny H

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  • LINC00707 promotes hepatocellular carcinoma progression through activating ERK/JNK/AKT pathway signaling pathway.

    abstract::Increasing evidence has demonstrated that abnormal expression of lncRNA is correlated with various malignant tumors, including hepatocellular carcinoma (HCC). Our current study was aimed to investigate the role of LINC00707 in HCC development. We observed that LINC00707 was upregulated in HCC cell lines compared with ...

    journal_title:Journal of cellular physiology

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    authors: Wang J,Luo Z,Yao T,Li W,Pu J

    更新日期:2019-05-01 00:00:00

  • Novel insights into Dhh signaling in antler chondrocyte proliferation and differentiation: Involvement of Foxa.

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    journal_title:Journal of cellular physiology

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    authors: Ma L,Duan CC,Yang ZQ,Ding JL,Liu S,Yue ZP,Guo B

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    journal_title:Journal of cellular physiology

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    journal_title:Journal of cellular physiology

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    authors: Martínez-López P,Treviño CL,de la Vega-Beltrán JL,De Blas G,Monroy E,Beltrán C,Orta G,Gibbs GM,O'Bryan MK,Darszon A

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Xia X,Cheng A,Lessor T,Zhang Y,Hamburger AW

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  • Hepatocyte growth factor enhances protein phosphatase Cdc25A inhibitor compound 5-induced hepatoma cell growth inhibition via Akt-mediated MAPK pathway.

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wang Z,Wang M,Carr BI

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    journal_title:Journal of cellular physiology

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    authors: Blay J,Brown KD

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    authors: Benedetti E,d'Angelo M,Ammazzalorso A,Gravina GL,Laezza C,Antonosante A,Panella G,Cinque B,Cristiano L,Dhez AC,Astarita C,Galzio R,Cifone MG,Ippoliti R,Amoroso R,Di Cesare E,Giordano A,Cimini A

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    abstract::COVID-19, a new disease caused by the 2019-novel coronavirus (SARS-CoV-2), has swept the world and challenged its culture, economy, and health infrastructure. Forced emergence to find an effective vaccine to immunize people has led scientists to design and examine vaccine candidates all over the world. Until a vaccine...

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


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  • Retinoic acid induced growth arrest of human breast carcinoma cells requires protein kinase C alpha expression and activity.

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    journal_title:Journal of cellular physiology

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kelley LL,Koury MJ,Bondurant MC

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    abstract::The phytohemagglutinin (PHA)-stimulated human lymphocytes demonstrated trace or no activity (dihydro) folate acid reductase using three methods including a radioassay, but demonstrated ample activity of thymidylate synthetase. This was true regardless of the day of harvest, (first through seventh) of the stimulated ly...

    journal_title:Journal of cellular physiology

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    authors: Haurani FI,Kardinal CG,Biermann WA

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chiba N,Masuda A,Yoshikai Y,Matsuguchi T

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    journal_title:Journal of cellular physiology

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    authors: Zhang S,Wu X,Diao P,Wang C,Wang D,Li S,Wang Y,Cheng J

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mishra M,Del Valle L,Otte J,Darbinian N,Gordon J

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ni S,Li C,Xu N,Liu X,Wang W,Chen W,Wang Y,van Wijnen AJ

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Julien MA,Haller CA,Wang P,Wen J,Chaikof EL

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  • Myocardial proteases and cardiac remodeling.

    abstract::Cardiac hypertrophy (CH), characterized by the enlargement of cardiomyocytes, fibrosis and apoptosis, is one of the leading causes of death worldwide. Despite the advances in cardiovascular research, there remains a need to further investigate the signaling pathways that mediate CH in order to identify novel therapeut...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Riaz S,Zeidan A,Mraiche F

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Gao LR,Wang G,Zhang J,Li S,Chuai M,Bao Y,Hocher B,Yang X

    更新日期:2018-09-01 00:00:00

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Boccellino M,Giuberti G,Quagliuolo L,Marra M,D'Alessandro AM,Fujita H,Giovane A,Abbruzzese A,Caraglia M

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Guo LL,Chen YJ,Wang T,An J,Wang CN,Shen YC,Yang T,Zhao L,Zuo QN,Zhang XH,Xu D,Wen FQ

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Klohs WD,Wilson JR,Weiser MM,Frankfurt O,Bernacki RJ

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    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Roy LM,Gittinger CK,Landreth GE

    更新日期:1991-01-01 00:00:00