Abstract:
:Cells are quite sensitive to a change of the extracellular pH and respond to it through detection of the H+/HCO3- level in extracellular fluid. However, little is known about molecular details induced by acidosis, such as intracellular pathways and gene expression. Here we describe properties of gene expression, protein interaction, and DNA binding activity of basic region leucine zipper (bZIP) transcription factor Maf and FosB during extracellular acidification. When cells were incubated with low pH medium, the expressions of small Maf proteins (MafG, MafK, and MafF) and FosB were clearly increased in an extracellular pH-dependent manner and expressed transiently with a peak after 1-2 h after stimulation. Immunofluorescence and protein binding studies indicated that MafG was partially co-localized with FosB in the nucleus and MafG can form heterodimers with FosB at extracellular pH 7.40. Moreover, we found that MafG-FosB complexes are able to bind to AP-1 consensus sequence, TGACTCA. To investigate whether extracellular acidification influences to dimerization and DNA binding activity of MafG and FosB, extracellular pH of cultured cells was decreased from 7.40 to 6.80. The decrease in extracellular pH led to enhanced dimerization of MafG with FosB leading to augmentation of the DNA binding activity of the heterodimer to AP-1 consensus sequence. Moreover, extracellular acidification induces mRNA expression of matrix metalloproteinase-1, one of the genes that are regulated by AP-1. These results suggest that MafG-FosB complexes are involved in transcriptional regulation in response to extracellular acidification.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Shimokawa N,Kumaki I,Qiu CH,Ohmiya Y,Takayama K,Koibuchi Ndoi
10.1002/jcp.20369keywords:
subject
Has Abstractpub_date
2005-10-01 00:00:00pages
77-85issue
1eissn
0021-9541issn
1097-4652journal_volume
205pub_type
杂志文章abstract::The 10th annual meeting of the Italian Society for Virology (SIV) comprised seven plenary sessions focused on: General virology and viral genetics; Virus-Host interaction and pathogenesis; Viral oncology; Emerging viruses and zoonotic, foodborne and environmental pathways of transmission; Viral immunology and vaccines...
journal_title:Journal of cellular physiology
pub_type: 临床试验,杂志文章
doi:10.1002/jcp.24055
更新日期:2012-07-01 00:00:00
abstract::K+ channels of the alveolar epithelium control the driving force acting on the ionic and solvent flow through the cell membrane contributing to the maintenance of cell volume and the constitution of epithelial lining fluid. In the present work, we analyze the effect of the Cl- channel inhibitors: (4-[(2-butyl-6,7-dich...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28396
更新日期:2019-08-01 00:00:00
abstract::The hypothesis that, in the case of clonal or low-density cultures, cells which do not readily proliferate are those that do not produce an extracellular matrix (ECM), while those that proliferate actively are cells that have retained their ability to produce it, has been tested using low-density vascular endothelial ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041090109
更新日期:1981-10-01 00:00:00
abstract::Adipose-derived stem cells (ADSCs) are a type of mesenchymal stem cells isolated from adipose tissue and have the ability to differentiate into adipogenic, osteogenic, and chondrogenic lineages. Despite their great therapeutic potentials, previous studies showed that ADSCs could enhance the proliferation and metastati...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29574
更新日期:2020-10-01 00:00:00
abstract::A new form of cell death has been observed. The death occurs at liquid-air interfaces when Tetrahymena cells are grown in a chemically defined medium (CDM) at low inocula. The cells die by lysis at the liquid-air interface (medium surface), which they reach due to negative gravitaxis as well as positive aerotaxis. Whe...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199610)169:1<139::AID-JCP1
更新日期:1996-10-01 00:00:00
abstract::Recent studies have suggested that interferons (IFNs) have an antifibrotic effect in the liver independent of their antiviral effect although its detailed mechanism remains largely unknown. Some microRNAs have been reported to regulate pathophysiological activities of hepatic stellate cells (HSCs). We performed analys...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22598
更新日期:2011-10-01 00:00:00
abstract::MicroRNAs (miRNAs) are a newly identified class of small regulatory non-coding endogenous RNAs that are ubiquitous from animals to plants and have pivotal functions in nearly all biological and metabolic processes. Increasing evidence shows that miRNAs are also new players regulating many protein-coding genes and spec...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.22234
更新日期:2010-11-01 00:00:00
abstract::AMP-activated protein kinase (AMPK) has recently emerged as a potential target for cancer therapy due to the observation that activation of AMPK inhibits tumor cell growth. It is well-known that androgen receptor (AR) signaling is a major driver for the development and progression of prostate cancer and that downregul...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24494
更新日期:2014-06-01 00:00:00
abstract::Pharmacogenomics, toxicogenomics, and small RNA expression analysis are three of the most active research topics in the biological, biomedical, pharmaceutical, and toxicological fields. All of these studies are based on gene expression analysis, which requires reference genes to reduce the variations derived from diff...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22725
更新日期:2011-10-01 00:00:00
abstract::Destructive bone diseases caused by osteolysis are increasing in incidence. They are characterized by an excessive imbalance of osteoclast formation and activation. During osteolysis, the activation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways are triggered by receptor ac...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27924
更新日期:2019-07-01 00:00:00
abstract::We have studied the tissue distribution of interleukin (IL) and hemopoietic colony-stimulating factor (CSF) transcripts in mice by S1-nuclease protection analysis. Accumulation of several of these mRNAs in response to intravenous injection of lipopolysaccharide (LPS) appears to occur in a tissue-specific fashion. IL-1...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041380107
更新日期:1989-01-01 00:00:00
abstract::Pathological calcification represents an event that consequently leads to a distinct elevation in the morbidity and mortality of patients with chronic kidney disease (CKD) in addition to strengthening its correlation with hyperphosphatemia. Epigenomic regulation by specific microRNAs (miRNAs) is reported to be involve...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28130
更新日期:2019-08-01 00:00:00
abstract::Balb/C3T3 cells entered the quiescent Go state following serum deprivation. On addition of fresh serum, more than 95% of the culture resumed growth, but with asynchronous kinetics. If hydroxyurea were added just before the first cells reached S phase, at at least 90% of the cells accumulated at the Gl/S border over th...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040900219
更新日期:1977-02-01 00:00:00
abstract::Chick embryo fibroblasts were maintained at confluency for up to 35 days in medium containing 0.5% or 0.75% fetal bovine serum or 2.5% or 5.0% horse serum. At weekly intervals cells were subcultured and serially propagated in medium containing 10% FBS until their replicative lifespans were completed. The results showe...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041070308
更新日期:1981-06-01 00:00:00
abstract::Fibroblast growth factors (FGFs) are soluble ligands important for embryonic patterning, limb and brain development, and stem cell proliferation. They activate specific receptors (FGFR) to elicit changes in gene expression and cellular responses such as proliferation, differentiation, and survival, but the extent to w...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20483
更新日期:2006-02-01 00:00:00
abstract::As a strategy to identify gene expression changes affected by human polynucleotide phosphorylase (hPNPase(old-35)), we performed gene expression analysis of HeLa cells in which hPNPase(old-35) was overexpressed. The observed changes were then compared to those of HO-1 melanoma cells in which hPNPase(old-35) was stably...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24645
更新日期:2014-12-01 00:00:00
abstract::Circular RNA TCF25 (circTCF25) has been proven to be upregulated in human malignancy and correlated with tumor process. Our study aimed to unravel whether circTCF25 dictates cellular activities of osteosarcoma cells and address the possible mechanisms associated with microRNA (miR). circTCF25 and miR-206 in clinical s...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29570
更新日期:2020-02-13 00:00:00
abstract::We report that cultured vascular endothelial cells release into the culture medium a vasoconstrictor peptide, a substance we call an endothelium-derived constricting factor (EDCF). Conditioned medium from cultured bovine aortic and pulmonary artery endothelial cells caused sustained, dose-dependent isometric constrict...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041320210
更新日期:1987-08-01 00:00:00
abstract::Liver regeneration involves not only hepatocyte replication but progenitor aggregation and scarring. Partial hepatectomy (PH), an established model for liver regeneration, reactivates transforming growth factor-β (TGF-β) signaling. Hepatic stellate cells (HSCs) are primarily responding cells for TGF-β and resident in ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29169
更新日期:2020-03-01 00:00:00
abstract::Bovine pulmonary artery endothelial cells in culture were used to assess the influence of oxygen tension on proteoglycan synthesis. Cells exposed to 3% O2 (hypoxia) for 72 h and then labeled with [35S]sulfate for 5 h accumulated significantly less [35S]proteoglycan in medium than cells exposed to 20% O2 (control). Thi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041260214
更新日期:1986-02-01 00:00:00
abstract::Our objective was to establish the existence of intercellular communication through gap junctions in synovial lining cells and in primary and passaged cultures of human synovial cells. Communication between cells was assessed using the nystatin perforated-patch method, fluorescent dye transfer, immunochemistry, transm...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(200007)184:1<110::AID-JCP1
更新日期:2000-07-01 00:00:00
abstract::Bradykinin (BK) is a potent short-lived effector belonging to a class of peptides known as kinins. It participates in inflammatory and vascular regulation and processes including angioedema, tissue permeability, vascular dilation, and smooth muscle contraction. BK exerts its biological effects through the activation o...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.10175
更新日期:2002-12-01 00:00:00
abstract::Cells of confluent cultures of the established pig renal epithelial line, LLC-PK1, accumulate alpha-methyl-D-glucoside against a concentration gradient. This transport system is strongly inhibited by phlorizin and 6-deoxy-D-glucose, moderately inhibited by phloretin, and only weakly inhibited by 3-0-methyl-D-glucose, ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041040311
更新日期:1980-09-01 00:00:00
abstract::Interleukin (IL)-4, which exhibits potent anti-inflammatory activities, is of potential therapeutic value in destructive arthropathies. To further define the response of human joint cells to IL-4, we analyzed the ability of this cytokine to modulate the effects of IL-1beta and growth factors. Freshly isolated chondroc...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10121
更新日期:2002-07-01 00:00:00
abstract::Ephrin A1 has a role in a variety of biological events, including cell proliferation, differentiation, migration, and angiogenesis. Ephrin A1 expression is abundant in trophoblasts and endometrial cells during the implantation period; however, its intracellular activities have not yet been reported in bovine endometri...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27275
更新日期:2019-04-01 00:00:00
abstract::Multiple studies have reported different methods in treating gestational diabetes mellitus (GDM); however, the relationship between miR-335-5p and GDM still remains unclear. Here, this study explores the effect of miR-335-5p on insulin resistance and pancreatic islet β-cell secretion via activation of the TGFβ signali...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27406
更新日期:2019-05-01 00:00:00
abstract::The mechanism of zinc (Zn) uptake by microvillous membrane vesicles prepared from human term placenta has been studied. The uptake was complex, with two processes being identified. In the first process, uptake was rapid, reaching equilibrium within 2 min, and was temperature dependent, with a Q10 of 1.5. Equilibrium Z...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041510312
更新日期:1992-06-01 00:00:00
abstract::The increased levels of c-Myc protein observed previously in an ovarian carcinoma cell line stably transfected to express HER2 has suggested a role for the HER2 pathway in c-Myc expression. Analysis of HER2-transfected cells stimulated with heregulin beta1 (HRG) revealed increased c-Myc protein levels but not a corres...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20012
更新日期:2004-07-01 00:00:00
abstract::We have previously shown that extracellular ATP acts as a mitogen via protein kinase C (PKC)-dependent and independent pathways (Wang, D., Huang, N., Gonzalez, F.A., and Heppel, L.A. Multiple signal transduction pathways lead to extracellular ATP-stimulated mitogenesis in mammalian cells. I. Involvement of protein kin...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041460320
更新日期:1991-03-01 00:00:00
abstract::CCN1 is highly expressed in cancer cells and has been identified in the secretome of bone marrow-derived mesenchymal stem cells (BM-MSC). Although secreted CCN1 is known to promote angiogenesis, its underlying mechanism remains unclear. Here, we examined whether our recently-established tonsil-derived MSC (T-MSC) secr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24690
更新日期:2015-01-01 00:00:00