Primary human articular chondrocytes, dedifferentiated chondrocytes, and synoviocytes exhibit differential responsiveness to interleukin-4: correlation with the expression pattern of the common receptor gamma chain.

Abstract:

:Interleukin (IL)-4, which exhibits potent anti-inflammatory activities, is of potential therapeutic value in destructive arthropathies. To further define the response of human joint cells to IL-4, we analyzed the ability of this cytokine to modulate the effects of IL-1beta and growth factors. Freshly isolated chondrocytes, dedifferentiated chondrocytes, and synoviocytes were treated with IL-4 before determination of nitric oxide (NO) and collagenase production in response to IL-1beta, or before proliferation assays in presence of IL-1beta, platelet-derived growth factor (PDGF), or transforming growth factor (TGF)-beta. IL-4 downregulated IL-1beta induced NO production in dedifferentiated chondrocytes and inhibited IL-1beta induced collagenase release, as well as IL-1beta and growth factor induced proliferation in dedifferentiated chondrocytes and synoviocytes. In contrast, IL-4 had no effect in freshly isolated primary chondrocytes and in cartilage explants. The lack of response to IL-4 in primary chondrocytes was associated with impaired signal transduction, as indicated by markedly decreased IL-4 dependent tyrosine phosphorylation of signal transducer and activator of transcription (STAT)-6. It also correlated with differences in the expression pattern of IL-4 receptor (IL-4R) subunits during chondrocyte dedifferentiation. Indeed, whereas the IL-4Ralpha and IL-13Ralpha' subunits were expressed in all cell types, expression of the common receptor gamma chain was restricted to freshly isolated chondrocytes. In conclusion, IL-4 downregulated IL-1beta-induced catabolic events and cell proliferation in dedifferentiated chondrocytes and synoviocytes, but had no effects in freshly isolated chondrocytes. The difference in IL-4 responsiveness between primary and dedifferentiated chondrocytes correlated with changes in proximal signaling events and in the expression pattern of IL-4R subunits during cell dedifferentiation.

journal_name

J Cell Physiol

authors

Guicheux J,Palmer G,Relic B,Mezin F,Caverzasio J,Apostolides P,Gauchat JF,Gabay C,Guerne PA

doi

10.1002/jcp.10121

keywords:

subject

Has Abstract

pub_date

2002-07-01 00:00:00

pages

93-101

issue

1

eissn

0021-9541

issn

1097-4652

journal_volume

192

pub_type

杂志文章
  • Nine hub genes as the potential indicator for the clinical outcome of diabetic nephropathy.

    abstract::Diabetic nephropathy (DN) is one of the most serious and dangerous chronic complications caused by diabetes mellitus, and the identification and development of novel biomarkers could be beneficial for the diagnosis and prognosis of DN patients. This study focused on researching the differently expressed pattern of the...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26958

    authors: Song X,Gong M,Chen Y,Liu H,Zhang J

    更新日期:2019-02-01 00:00:00

  • microRNA-33-3p involved in selenium deficiency-induced apoptosis via targeting ADAM10 in the chicken kidney.

    abstract::Selenium (Se) deficiency induces typical clinical and pathological changes and causes various pathological responses at the molecular level in several different chicken organs; the kidney is one of the target organs of Se deficiency. To explore the mechanisms that underlie the effects of microRNA-33-3p (miR-33-3p) on ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28050

    authors: Wan N,Xu Z,Chi Q,Hu X,Pan T,Liu T,Li S

    更新日期:2019-08-01 00:00:00

  • Inhibition of BALB/c-3T3 cells in late G1: commitment to DNA synthesis controlled by somatomedin C.

    abstract::Methylglyoxal bis-(guanylhydrazone) (mGBG) blocked the stimulation of DNA synthesis in quiescent, density-inhibited BALB/c-3T3 cells treated with platelet-derived growth factor (PDGF) and platelet-poor plasma (PPP). Competence formation produced by a transient exposure to PDGF was not effected by mGBG. In contrast, mG...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041070105

    authors: Wharton W,Van Wyk JJ,Pledger WJ

    更新日期:1981-04-01 00:00:00

  • TGF-β1 enhances Kv2.1 potassium channel protein expression and promotes maturation of cerebellar granule neurons.

    abstract::Members of the transforming growth factor-β (TGF-β) family of cytokines are involved in diverse physiological processes. Although TGF-β is known to play multiple roles in the mammalian central nervous system (CNS), its role in neuronal development has not been explored. We have studied the effects of TGF-β1 on the ele...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22735

    authors: Zhuang JL,Wang CY,Zhou MH,Duan KZ,Mei YA

    更新日期:2012-01-01 00:00:00

  • Targeting IL-34 in inflammatory autoimmune diseases.

    abstract::Interleukin-34 (IL-34) shares a common receptor with macrophage colony-stimulating factor (M-CSF), and can bind to CSF-1R, induces lymphocytes differentiation, proliferation, and regulates the synthesis of inflammatory components. Recent findings reported aberrant expression of IL-34 in several autoimmune disorders, s...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.28946

    authors: Xu WD,Huang AF,Fu L,Liu XY,Su LC

    更新日期:2019-12-01 00:00:00

  • Growth kinetics as a function of ploidy in diploid, tetraploid, and octaploid smooth muscle cells derived from the normal rat aorta.

    abstract::The smooth muscle cell population in major arteries of humans and experimental animals is heterogeneous with regard to cellular DNA content. A proportion of cells has polyploid DNA content and this proportion increases with normal aging and with hypertension. We have isolated pure populations of rat aortic smooth musc...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041250322

    authors: Rosen EM,Goldberg ID,Shapiro HM,Zoller LC,Myrick KV,Levenson SE,Halpin PA

    更新日期:1985-12-01 00:00:00

  • Inflammatory cytokines in bladder cancer.

    abstract::The presence of inflammatory cells and their products in the tumor microenvironment plays a crucial role in the pathogenesis of a tumor. Releasing the cytokines from a host in response to infection and inflammation can inhibit tumor growth and progression. However, tumor cells can also respond to the host cytokines wi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.28252

    authors: Shadpour P,Zamani M,Aghaalikhani N,Rashtchizadeh N

    更新日期:2019-02-18 00:00:00

  • Brg1, the ATPase subunit of the SWI/SNF chromatin remodeling complex, is required for myeloid differentiation to granulocytes.

    abstract::Many mammalian SWI/SNF complexes use Brahma-related gene 1 (Brg1) as a catalytic subunit to remodel nucleosomes for transcription regulation. In several mesenchymal cells and tissues, expression of a defective Brg1 protein negates the normal activity of the SWI/SNF complex and delays or blocks differentiation. To inve...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20432

    authors: Vradii D,Wagner S,Doan DN,Nickerson JA,Montecino M,Lian JB,Stein JL,van Wijnen AJ,Imbalzano AN,Stein GS

    更新日期:2006-01-01 00:00:00

  • Flavonoid genistein protects bone marrow sinusoidal blood vessels from damage by methotrexate therapy in rats.

    abstract::Cancer chemotherapy can cause significant damage to the bone marrow (BM) microvascular (sinusoidal) system. Investigations must now address whether and how BM sinusoidal endothelial cells (SECs) can be protected during chemotherapy. Herein we examined the potential protective effects of genistein, a soy-derived flavon...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27785

    authors: Hassanshahi M,Su YW,Khabbazi S,Fan CM,Chen KM,Wang JF,Qian A,Howe PR,Yan DW,Zhou HD,Xian CJ

    更新日期:2019-07-01 00:00:00

  • Induction of heat-shock response and alterations of protein phosphorylation by a novel topoisomerase II inhibitor, withangulatin A, in 9L rat brain tumor cells.

    abstract::Withangulatin A is a newly identified in vitro topoisomerase II inhibitor isolated from the Chinese antitumor herb Physalis angulata. In vivo, it was found to be cytotoxic, capable of suppressing general protein synthesis and of inducing the synthesis of a small set of proteins including those generated by heat-shock ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041490110

    authors: Lee WC,Lin KY,Chen CM,Chen ZT,Liu HJ,Lai YK

    更新日期:1991-10-01 00:00:00

  • Challenges and opportunities of metabolomics.

    abstract::The metabolome is a data-rich source of information concerning all the low-molecular-weight metabolites in a biofluid, which can indicate early biological changes to the host due to perturbations in metabolic pathways. Major changes can be seen after minor stimuli, which make it a valuable target for analysis. Due to ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.24002

    authors: Johnson CH,Gonzalez FJ

    更新日期:2012-08-01 00:00:00

  • Electrophysiological characterization of voltage-gated Na+ current expressed in the highly metastatic Mat-LyLu cell line of rat prostate cancer.

    abstract::Voltage-gated Na+ channels, classically associated with impulse conduction in excitable tissues, are also found in a variety of epithelial cell types where their possible functions are not known so well. We have previously reported expression of a voltage-gated Na+ channel specifically in the highly metastatic Mat-LyL...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199804)175:1<50::AID-JCP6>

    authors: Grimes JA,Djamgoz MB

    更新日期:1998-04-01 00:00:00

  • Thrombin induces expression of twist and cell motility via the hypoxia-inducible factor-1α translational pathway in colorectal cancer cells.

    abstract::Deep vein thrombosis associated with advanced cancer is known as Trousseau's syndrome. We hypothesized that thrombin, an activator of protease-activated receptor (PAR)-1 and PAR-4 contributes to tumor metastasis. In this study, we demonstrated that thrombin and the PAR-1 activating peptide (AP) SFLLRN, but not the PAR...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22428

    authors: Chang LH,Chen CH,Huang DY,Pai HC,Pan SL,Teng CM

    更新日期:2011-04-01 00:00:00

  • Adaptation of potassium metabolism and restoration of mitosis during prolonged treatment of mouse lymphoblasts with ouabain.

    abstract::The effects of ouabain on the growth of murine lymphoblasts in vitro have been studied. Exposure of cells to ouabain (0.1 mM) initially inhibited 86Rb+ uptake rate, reduced the intracellular potassium concentration, and decreased population growth rates. Continued exposure to the same ouabain concentration resulted in...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040850208

    authors: Cuff JM,Lichtman MA

    更新日期:1975-04-01 00:00:00

  • Acute exercise reduces insulin resistance-induced TRB3 expression and amelioration of the hepatic production of glucose in the liver of diabetic mice.

    abstract::TRB3 (a mammalian homolog of Drosophila) is emerging as an important player in the regulation of insulin signaling. TRB3 can directly bind to Ser/Thr protein kinase Akt, the major downstream kinase of insulin signaling. Conversely, physical exercise has been linked to improved glucose homeostasis and enhanced insulin ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21833

    authors: Lima AF,Ropelle ER,Pauli JR,Cintra DE,Frederico MJ,Pinho RA,Velloso LA,De Souza CT

    更新日期:2009-10-01 00:00:00

  • Selective release from cultured mammalian cells of heat-shock (stress) proteins that resemble glia-axon transfer proteins.

    abstract::Cultured rat embryo cells were stimulated to rapidly release a small group of proteins that included several heat-shock proteins (hsp110, hsp71, hscp73) and nonmuscle actin. The extracellular proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. Heat-shocked cells released the same set of prote...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041380206

    authors: Hightower LE,Guidon PT Jr

    更新日期:1989-02-01 00:00:00

  • Apelin/APJ system: A novel promising target for neurodegenerative diseases.

    abstract::Neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease (PD), and Huntington's disease (HD), are incurable diseases characterized by progressive loss of cognitive or motor function, which construct a serious threat to the life quality of aging populations and their life spans. Apelin is an ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.29001

    authors: Luo H,Han L,Xu J

    更新日期:2020-02-01 00:00:00

  • A new insight into thymosin β4, a promising therapeutic approach for neurodegenerative disorders.

    abstract::Thymosin β4 (Tβ4), a G-actin-sequestering secreted peptide, improves neurovascular remodeling and central nervous system plasticity, which leads to neurological recovery in many neurological diseases. Inflammatory response adjustment and tissue inflammation consequences from neurological injury are vital for neurologi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.29293

    authors: Shomali N,Baradaran B,Deljavanghodrati M,Akbari M,Hemmatzadeh M,Mohammadi H,Jang Y,Xu H,Sandoghchian Shotorbani S

    更新日期:2020-04-01 00:00:00

  • A critical temperature transition of K+-Na+ exchange in human lymphocytes.

    abstract::Human lymphocytes were equilibrated for 48 hours at 5-6 mM K+ ex over a range of temperatures between 0 and 37 degrees C, and at 5 degrees C over a range of external K+ levels between 0 and 32 mM. Cell K+ and Na+ contents are normal between 37 and 10 decrees. Below 10 degrees there is a critical thermal transition in ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041030113

    authors: Negendank W,Shaller C

    更新日期:1980-04-01 00:00:00

  • Direct conversion of somatic cells towards oligodendroglial lineage cells: A novel strategy for enhancement of myelin repair.

    abstract::Oligodendrocyte precursor cells (OPCs) are considered as the main cell source for myelination in the central nervous system. Following demyelination, proliferation, migration, and differentiation capability of endogenous OPCs remarkably increase leading to remyelination in damaged areas. Despite the beneficial impacts...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.29195

    authors: Yavarpour-Bali H,Nakhaei-Nejad M,Yazdi A,Ghasemi-Kasman M

    更新日期:2020-03-01 00:00:00

  • Induced PAI-1 mRNA expression and targeted protein accumulation are early G1 events in serum-stimulated rat kidney cells.

    abstract::Expression of plasminogen activator inhibitor type-1 (PAI-1), a member of the SERPIN gene family that functions to regulate the plasmin-based pericellular proteolytic cascade, is growth state-regulated in normal rat kidney (NRK) cells (Ryan and Higgins, 1990, J. Cell. Physiol., 155:376-384; Ryan et al., 1996, Biochem....

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199701)170:1<8::AID-JCP2>3

    authors: Kutz SM,Nickey SA,White LA,Higgins PJ

    更新日期:1997-01-01 00:00:00

  • INI1 expression induces cell cycle arrest and markers of senescence in malignant rhabdoid tumor cells.

    abstract::The INI1 gene, which encodes a functionally uncharacterized protein component of the hSWI/SNF chromatin remodeling complex, is often mutated or deleted in malignant rhabdoid tumor (MRT). Two isoforms of INI1, that differ by the variable inclusion of nine amino acids, potentially are produced by differential RNA splici...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.10201

    authors: Reincke BS,Rosson GB,Oswald BW,Wright CF

    更新日期:2003-03-01 00:00:00

  • Effect of strain on human keratinocytes in vitro.

    abstract::Tissue expansion, a technique to enlarge the skin surface area with an expandable balloon, has been widely used in reconstructive surgery. Although the effect of tissue expansion on in vivo skin physiology and histology has been well documented, it remains unclear whether keratinocytes or other cell types are responsi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199710)173:1<64::AID-JCP8>

    authors: Takei T,Rivas-Gotz C,Delling CA,Koo JT,Mills I,McCarthy TL,Centrella M,Sumpio BE

    更新日期:1997-10-01 00:00:00

  • Rapamycin-sensitive phase of 3T3-L1 preadipocyte differentiation after clonal expansion.

    abstract::Inhibition of insulin-induced 3T3-L1 preadipocyte differentiation by rapamycin has been attributed to a blockade of the early critical clonal expansion phase of the adipogenic program. Rapamycin binds to, and inhibits, mTOR (mammalian target of rapamycin), leading to diminution of p70 S6 kinase activity and eukaryotic...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1132

    authors: Gagnon A,Lau S,Sorisky A

    更新日期:2001-10-01 00:00:00

  • Curcumin, hemostasis, thrombosis, and coagulation.

    abstract::Atherothrombotic cardiovascular disease is a major cause of mortality throughout the world. Platelet activation and aggregation play a central role in hemostasis and thrombosis. Herbal medicines have been traditionally used in the management of cardiovascular disease and can help in modifying its progression, particul...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.26249

    authors: Keihanian F,Saeidinia A,Bagheri RK,Johnston TP,Sahebkar A

    更新日期:2018-06-01 00:00:00

  • Cell cycle changes in transformed cells growing under serum-free conditions.

    abstract::Polyoma transformed hamster cells (PyBHK) and SV40 transformed mouse cells (SV3T3) were transferred in culture using crystalline trypsin followed by neutralisation with soybean trypsin inhibitor. Such cells were able to proliferate freely in defined medium without any serum supplement and without any intervening perio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040900319

    authors: Bush H,Shodell M

    更新日期:1977-03-01 00:00:00

  • Intelligent TAT-coupled anti-HER2 immunoliposomes knock downed MDR1 to produce chemosensitize phenotype of multidrug resistant carcinoma.

    abstract::Gene therapy using biocompatible cationic liposomes is amongst promising approaches that decreases death from cancers. Here an invasive multidrug resistant cell model has been developed by lentiviral transfection. In parallel phospholipids have been covalently conjugated to TAT, MMP2, and Herceptin. The functional lip...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28683

    authors: Gholamian Dehkordi N,Elahian F,Khosravian P,Mirzaei SA

    更新日期:2019-11-01 00:00:00

  • Regulation of programmed death in erythroid progenitor cells by erythropoietin: effects of calcium and of protein and RNA syntheses.

    abstract::Erythropoietin (EPO) retards DNA breakdown characteristic of programmed cell death (apoptosis) and promotes survival in erythroid progenitor cells. The mechanism by which EPO inhibits programmed death is unknown. In the well-characterized model of glucocorticoid-treated thymocytes, activation of a Ca2+/Mg(2+)-sensitiv...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041510307

    authors: Kelley LL,Koury MJ,Bondurant MC

    更新日期:1992-06-01 00:00:00

  • Differential localization and expression of the Cdk9 42k and 55k isoforms.

    abstract::Cdk9, a member of the cyclin-dependent kinase family, is the catalytic subunit of P-TEFb, a protein kinase complex that stimulates transcriptional elongation. Cdk9, complexed with its regulatory partner cyclin T1, serves as the cellular mediator of the transactivation function of the HIV Tat protein. There are two kno...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20224

    authors: Liu H,Herrmann CH

    更新日期:2005-04-01 00:00:00

  • Ascorbic Acid Induces Necrosis in Human Laryngeal Squamous Cell Carcinoma via ROS, PKC, and Calcium Signaling.

    abstract::Ascorbic acid induces apoptosis, autophagy, and necrotic cell death in cancer cells. We investigated the mechanisms by which ascorbic acid induces death in laryngeal squamous cell carcinoma Hep2 cells. Ascorbic acid markedly reduced cell viability and induced death without caspase activation and an increase in cytochr...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25438

    authors: Baek MW,Cho HS,Kim SH,Kim WJ,Jung JY

    更新日期:2017-02-01 00:00:00