Abstract:
:Human population is facing a revolutionary change in the demographic structure with an increasing number of elderly people requiring an unmet need to ensure a smooth aging process and dental care is certainly an important aspect that has to be considered. To date, dentistry has been conservative and the need of transferring the scientific models of regenerative dentistry into clinical practice is becoming a necessity. The aim of this study was to characterize the differentiation commitment (in vitro) and the clinical grafting ability (in vivo) of a population of progenitor stem cells obtained after mechanical digestion of dental pulp with an innovative system recently developed. This approach was successfully used in previous studies to obtain a clinical-grade ready to use dental pulp fragments that could be grafted in autologous tissues to obtain bone. We are thus showing that micro grafts resulting from mechanical digestion contain stem cells with a mesenchymal phenotype, able to differentiate toward different cell types and to generate new bone in patients. We are providing data for the establishment of standardized and routinely oral surgery approaches, having outlined the cellular properties of human stem cells obtained from the dental pulp. This method can represent a valid tool for both regenerative medicine and tissue engineering purposes not only applicable to the cranio-maxillofacial region but, likely, to different bone pathologies for a fastening and healing recovering of patients. J. Cell. Physiol. 232: 548-555, 2017. © 2016 Wiley Periodicals, Inc.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Monti M,Graziano A,Rizzo S,Perotti C,Del Fante C,d'Aquino R,Redi CA,Rodriguez Y Baena Rdoi
10.1002/jcp.25452subject
Has Abstractpub_date
2017-03-01 00:00:00pages
548-555issue
3eissn
0021-9541issn
1097-4652journal_volume
232pub_type
杂志文章abstract::Scientists of numerous medical and life science disciplines met in Siena, Italy to discuss the latest proceedings in basic and clinical research. General models of interconnected linear and back-feeding cell-cycle control pathways provide a basis for applied molecular research. Cell-cycle determining factors essential...
journal_title:Journal of cellular physiology
pub_type:
doi:10.1002/1097-4652(200012)185:3<481::AID-JCP20>3.0.
更新日期:2000-12-01 00:00:00
abstract::We have previously shown that extracellular ATP acts as a mitogen via protein kinase C (PKC)-dependent and independent pathways (Wang, D., Huang, N., Gonzalez, F.A., and Heppel, L.A. Multiple signal transduction pathways lead to extracellular ATP-stimulated mitogenesis in mammalian cells. I. Involvement of protein kin...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041460320
更新日期:1991-03-01 00:00:00
abstract::Recent data indicate that the Simian virus 40 (SV40) infection appears to be transmitted in humans independently from early SV40-contaminated antipolio vaccines. Serum antibodies against SV40 large T antigen (Tag) were analyzed in children/adolescents and young adults. To investigate antibodies reacting to SV40 Tag an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27490
更新日期:2019-04-01 00:00:00
abstract::Earlier studies in multiple epithelia have shown that cell-cell actin-based adherens junction (AJ) dynamics are regulated, at least in part, by the interplay of kinases and phosphatases that determines the intracellular phosphoprotein content. Yet it is virtually unknown regarding the role of protein kinases in Sertol...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20119
更新日期:2005-02-01 00:00:00
abstract::BAG3 protein, a member of the BAG co-chaperones family, sustains cell survival in a variety of normal and neoplastic cell types, via its interaction with a variety of partners, such as the heat shock protein (HSP) 70, Bcl-2, Raf-1 and others. Expression of BAG3 is induced by some stressful stimuli, such as heat shock,...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21634
更新日期:2009-03-01 00:00:00
abstract::We have previously found progressive diabetic nephropathy in inducible cAMP early repressor (ICER Igamma) transgenic (Tg) mice. The ICER Igamma Tg mouse is an interesting model of sustained hyperglycemia due to its low production of insulin and insulin-producing beta cells. Here in a longitudinal study we further anal...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21316
更新日期:2008-05-01 00:00:00
abstract::We performed a network meta-analysis (NMA) to compare the short- and long-term efficacy of Gemcitabine, Gemcitabine + S-1 (tegafur), Gemcitabine + nab-paclitaxel, Gemcitabine + Capecitabine, Gemcitabine + Cisplatin, FOLFIRINOX (oxaliplatin + irinotecan + fluorouracil + leucovorin), Gemcitabine + oxaliplatin, Gemcitabi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,meta分析
doi:10.1002/jcp.26183
更新日期:2018-04-01 00:00:00
abstract::We report that cultured vascular endothelial cells release into the culture medium a vasoconstrictor peptide, a substance we call an endothelium-derived constricting factor (EDCF). Conditioned medium from cultured bovine aortic and pulmonary artery endothelial cells caused sustained, dose-dependent isometric constrict...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041320210
更新日期:1987-08-01 00:00:00
abstract::It has been established that syndecan-1 is an important modulator of events relevant to acute tissue repair and chronic injury responses. The current studies were designed to examine syndecan-1 expression during atherosclerotic lesion formation and whether angiotensin II influences syndecan-1 expression in macrophages...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21271
更新日期:2008-03-01 00:00:00
abstract::Neuropathological and clinical evidence indicates that the clinical expression of Alzheimer's disease (AD) occurs as neuropathology exceeds the brain reserve capacity. The brain or cognitive reserve (BCR) hypothesis states that high premorbid intelligence, education, and an active and stimulating lifestyle provide res...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.28308
更新日期:2019-02-19 00:00:00
abstract::The 94-kDa glucose-regulated protein (GRP94) is a glycoprotein in the endoplasmic reticulum (ER). It has been characterized as a Ca2+-binding protein and a molecular chaperone. In this report we show that highly purified GRP94 exhibits an active Mg2+-dependent serine kinase activity (termed 94-kinase). The 94-kinase c...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199702)170:2<115::AID-JCP3
更新日期:1997-02-01 00:00:00
abstract::Human fibroblasts derived from newborn foreskin and designated CF-3 were assayed for heat labile glucose-6-phosphate dehydrogenase (G6PD) when they had grown to confluency, as well as when they were arrested in an essentially nonmitotic state. Under both culture conditions there was an increase in heat labile G6PD (up...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040930108
更新日期:1977-10-01 00:00:00
abstract::Mouse epidermal cells can be grown as a proliferating monolayer in medium containing 0.02-0.1 mM calcium. Terminal differentiation of these cells with formation of cornified cells and cell death is induced by elevating calcium in the medium to greater than 0.1 mM. A variety of agents were studied as potential modifier...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041160303
更新日期:1983-09-01 00:00:00
abstract::We have proposed that the transmembrane receptor encoded by the c-Kit protooncogene and its ligand play an important role in regulating the proliferation of blasts cells in acute myeloblastic leukemia (AML). To test this hypothesis, immunobeads were used to separate blasts from three Kit-expression positive cell lines...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041540225
更新日期:1993-02-01 00:00:00
abstract::Conditions have been established under which the antizyme of ornithine decarboxylase (E.C. 4.1.1.17, L-ornithine carboxy-lyase, ODC) a non-competitive protein inhibitor of ODC, can be detected in cells in response to as little as 10(-7) M putrescine. The maintenance of intracellular antizyme activity depends upon the ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041070206
更新日期:1981-05-01 00:00:00
abstract::Beta cell apoptosis is a hallmark of diabetes. Since we have previously shown that galectin-3 deficient (LGALS3(-/-) ) mice are relatively resistant to diabetes induction, the aim of this study was to examine whether beta cell apoptosis depends on the presence of galectin-3 and to delineate the underlying mechanism. D...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24318
更新日期:2013-07-01 00:00:00
abstract::Hyperhomocysteinemia (HHcy) is associated with atherosclerotic events involving the modulation of arachidonic acid (AA) metabolism and the activation of matrix metalloproteinase-9 (MMP-9). Cytochrome P450 (CYP) epoxygenase-2J2 (CYP2J2) is abundant in the heart endothelium, and its AA metabolites epoxyeicosatrienoic ac...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21356
更新日期:2008-06-01 00:00:00
abstract::We previously showed that fluid flow, which chondrocytes experience in vivo and which results in a variety of morphological and metabolic changes in cultured articular chondrocytes, can also stimulate a rise in intracellular calcium concentration ([Ca2+]i). However, the mechanism by which Ca2+ is mobilized in response...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199909)180:3<402::AID-JCP1
更新日期:1999-09-01 00:00:00
abstract::Ischemia is a situation occurring in several diseases including myocardial infarction and organ transplantation in which oxygenated blood supply is impaired. Ischemia leads to many cellular and tissue modifications, the most important one being cell death. Several explanations have been proposed to account for these m...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041520127
更新日期:1992-07-01 00:00:00
abstract::Human cell lines derived from a melanoma and a colon carcinoma, and cultures of human melanocytes and intestinal epithelial cells, as well as a mouse mesenchymal non-neoplastic cell line and a malignant subline of the same have been quantitatively studied in tissue culture for their sensitivity to thymidine. All three...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040920308
更新日期:1977-09-01 00:00:00
abstract::Temperature-sensitive mutants have revealed a region of the herpes simplex virus 1 genome that affects both the uptake of hexose and the synthesis of heat shock proteins. Other inducers of heat-shock proteins, namely heat shock itself and arsenite, likewise induce an increased uptake of hexose. The increased uptake, l...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041280306
更新日期:1986-09-01 00:00:00
abstract::Polyoma transformed hamster cells (PyBHK) and SV40 transformed mouse cells (SV3T3) were transferred in culture using crystalline trypsin followed by neutralisation with soybean trypsin inhibitor. Such cells were able to proliferate freely in defined medium without any serum supplement and without any intervening perio...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040900319
更新日期:1977-03-01 00:00:00
abstract::Drug discovery is an onerous, extremely expensive, and time-consuming process. Instead, drug repurposing is an attractive strategy for exploiting novel indications for a drug beyond its original use. The untapped potential of drug repurposing compensates the barriers associated with the drug discovery pipeline. Psoria...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29413
更新日期:2020-06-01 00:00:00
abstract::Neurofibromatosis type 1 (NF1) is characterized by the formation of neurofibromas, benign tumors of the peripheral nerve consisting essentially of Schwann cells, which can sometimes turn malignant to form neurofibrosarcomas. The mechanism of progression toward a malignant phenotype remains largely unknown. In this rep...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199811)177:2<334::AID-JCP1
更新日期:1998-11-01 00:00:00
abstract::Hepatocyte spheroids have been proposed for drug metabolism studies and in bioartificial liver devices. However, the optimal conditions required to meet the aerobic demands of mitochondria-rich hepatocyte spheroids is not well studied. We hypothesized that an optimal concentration of oxygen could be identified and tha...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22651
更新日期:2011-11-01 00:00:00
abstract::Stem cells are a unique class of cells that are capable of self-renewal and differentiation into multiple lineages. An increasing number of studies have suggested that both embryonic and adult stem cells possess primary cilia, antenna-like structures protruding from cell surfaces that are critical for sensing and tran...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
doi:10.1002/jcp.25683
更新日期:2017-05-01 00:00:00
abstract::Muc4 (also called Sialomucin complex) is a heterodimeric glycoprotein complex consisting of a peripheral O-glycosylated subunit ASGP-1 (ascites sialoglycoprotein-1) tightly but non-covalently bound to an N-glycosylated transmembrane subunit ASGP-2. Muc4/SMC can act as an intramembrane ligand for ErbB2 via an EGF-like ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20200
更新日期:2005-04-01 00:00:00
abstract::The Schneider membrane is the mucosa that covers the inner part of the maxillary sinus cavities. The free surface is a ciliated pseudostratified epithelium, while the deeper portion is a highly vascularized connective tissue. The stromal fraction, bordering the bony wall of the sinus, after tooth loss can exhibit incr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24022
更新日期:2012-09-01 00:00:00
abstract::Evolving data suggest that marrow hematopoietic stem cells show reversible changes in homing, engraftment, and differentiation phenotype with cell cycle progression. Furthermore, marrow stem cells are a cycling population. Traditional concepts hold that the system is hierarchical, but the information on the lability o...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21273
更新日期:2008-03-01 00:00:00
abstract::The herbicide 2,4,5 trichlorophenoxy acetic acid (245T) at concentrations from 0.5 to 0.9 mM, was found to inhibit respiration and then growth in exponentially growing cultures of Tetrahymena pyriformis. Cell division was stopped for periods up to 60 minutes after which the cells recovered and division resumed. Recove...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040850219
更新日期:1975-04-01 00:00:00