Quercetin protects against radiocontrast medium toxicity in human renal proximal tubular cells.


:Radiocontrast media (RCM)-induced acute kidney injury (CI-AKI) is a major clinical problem whose pathophysiology is not well understood. Direct toxic effects on renal cells, possibly mediated by reactive oxygen species, have been postulated as contributing to CI-AKI. We investigated the effect of quercetin on human renal proximal tubular (HK-2) cells treated with the radiocontrast medium (RCM) sodium diatrizoate. Quercetin is the most widely studied flavonoid, and the most abundant flavonol present in foods. It has been suggested to have many health benefits, including angioprotective properties and anti-cancer effects. These beneficial effects have been attributed to its antioxidant properties and its ability to modulate cell signaling pathways. Incubation of HK-2 cells with 100 μM quercetin caused a decrease in cell viability and pre-treatment of HK-2 cells with 100 μM quercetin followed by incubation with 75 mgI/ml sodium diatrizoate for 2 hr caused a decrease in cell viability which was worse than in cells treated with diatrizoate alone. However, further incubation of the cells (for 22 hr) after removal of the diatrizoate and quercetin caused a recovery in cell viability in those cells previously treated with quercetin + diatrizoate and quercetin alone. Analysis of signaling molecules by Western blotting showed that in RCM-treated cells receiving initial pre-treatment with quercetin, followed by its removal, an increase in phosphorylation of Akt (Ser473), pSTAT3 (Tyr705), and FoxO3a (Thr32) as well as an induction of Pim-1 and decrease in PARP1 cleavage were observed. Quercetin may alleviate the longer-term toxic effects of RCM toxicity and its possible beneficial effects should be further investigated.


J Cell Physiol


Andreucci M,Faga T,Pisani A,Serra R,Russo D,De Sarro G,Michael A




Has Abstract


2018-05-01 00:00:00












  • Macrophage plasticity, polarization, and function in health and disease.

    abstract::Macrophages are heterogeneous and their phenotype and functions are regulated by the surrounding micro-environment. Macrophages commonly exist in two distinct subsets: 1) Classically activated or M1 macrophages, which are pro-inflammatory and polarized by lipopolysaccharide (LPS) either alone or in association with Th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Shapouri-Moghaddam A,Mohammadian S,Vazini H,Taghadosi M,Esmaeili SA,Mardani F,Seifi B,Mohammadi A,Afshari JT,Sahebkar A

    更新日期:2018-09-01 00:00:00

  • Glucose concentration and streptomycin alter in vitro muscle function and metabolism.

    abstract::Cell culture conditions can vary between laboratories and have been optimised for 2D cell culture. In this study, engineered muscle was cultured in 5.5 mM low glucose (LG) or 25 mM high glucose (HG) and in the absence or presence (+S) of streptomycin and the effect on C2C12 tissue-engineered muscle function and metabo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Khodabukus A,Baar K

    更新日期:2015-06-01 00:00:00

  • Cellular Metabolism and Dose Reveal Carnitine-Dependent and -Independent Mechanisms of Butyrate Oxidation in Colorectal Cancer Cells.

    abstract::Dietary fiber has been suggested to suppress colorectal cancer development, although the mechanisms contributing to this beneficial effect remain elusive. Butyrate, a fermentation product of fiber, has been shown to have anti-proliferative and pro-apoptotic effects on colorectal cancer cells. The metabolic fate of but...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Han A,Bennett N,MacDonald A,Johnstone M,Whelan J,Donohoe DR

    更新日期:2016-08-01 00:00:00

  • 15-Lipoxygenase promotes chronic hypoxia-induced phenotype changes of PASMCs via positive feedback-loop of BMP4.

    abstract::Our laboratory has previously demonstrated that 15-lipoxygenase (15-LO)/15-hydroxyeicosatetr-aenoic acid (15-HETE) is involved in hypoxic pulmonary arterial hypertension (PAH). Phenotypical alterations of vascular smooth muscle cells are considered to be an important stage in the development of PAH, whereas the underl...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yu X,Wei L,Lu P,Shen T,Liu X,Li T,Zhang B,Yu H,Zhu D

    更新日期:2015-07-01 00:00:00

  • Identification of murine phosphodiesterase 5A isoforms and their functional characterization in HL-1 cardiac cell line.

    abstract::Phosphodiesterase 5A (PDE5A) specifically degrades the ubiquitous second messenger cGMP and experimental and clinical data highlight its important role in cardiac diseases. To address PDE5A role in cardiac physiology, three splice variants of the PDE5A were cloned for the first time from mouse cDNA library (mPde5a1, m...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Campolo F,Zevini A,Cardarelli S,Monaco L,Barbagallo F,Pellegrini M,Cornacchione M,Di Grazia A,De Arcangelis V,Gianfrilli D,Giorgi M,Lenzi A,Isidori AM,Naro F

    更新日期:2018-01-01 00:00:00

  • PML down-regulation in soft tissue sarcomas.

    abstract::To date, little is known concerning the promyelocytic leukemia gene (PML) status in tumors of different origin, and its expression has never been evaluated in soft tissue sarcoma. The aim of the present study is focused on the identification of differences in terms of PML protein expression between different types of ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Vincenzi B,Perrone G,Santini D,Grosso F,Silletta M,Frezza A,Rossi S,Russo A,Rabitti C,Gebbia N,Badalamenti G,Casali P,Muda AO,Dei Tos AP,Tonini G

    更新日期:2010-09-01 00:00:00

  • Regulation of Puralpha gene transcription: evidence for autoregulation of Puralpha promoter.

    abstract::The single-stranded DNA and RNA binding protein, Puralpha, has recently received special attention as this protein, by associating with the specific nucleotide sequence (GGN repeats) and/or several important cellular and viral proteins regulates crucial biological events such as transcription, replication, and cell pr...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Muralidharan V,Sweet T,Nadraga Y,Amini S,Khalili K

    更新日期:2001-03-01 00:00:00

  • Fibronectin and fibrinolysis are not required for fibrin gel contraction by human skin fibroblasts.

    abstract::Human skin fibroblasts contracted fibrin gels in a time- and cell-dependent manner. Under optimal conditions, gel contraction amounted to more than 50% in 2 hr. Fibronectin did not promote contraction, and fibrinolysis was not required for contraction, although gels contracted without serum or aprotinin were lysed. Be...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tuan TL,Grinnell F

    更新日期:1989-09-01 00:00:00

  • Down-regulation of epidermal growth factor receptor induced by estrogens and phytoestrogens promotes the differentiation of U2OS human osteosarcoma cells.

    abstract::In previous studies on HeLa cells we demonstrated estrogen-responsiveness of the epidermal growth factor receptor (EGFR) gene, as 17beta-estradiol (E(2)) and selective estrogen receptor modulators (SERMs) genistein (G), daidzein (D), and 4-hydroxytamoxifen (4OH-T) modulated its transcription in a ligand- and estrogen ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Salvatori L,Caporuscio F,Coroniti G,Starace G,Frati L,Russo MA,Petrangeli E

    更新日期:2009-07-01 00:00:00

  • Curcumin, hemostasis, thrombosis, and coagulation.

    abstract::Atherothrombotic cardiovascular disease is a major cause of mortality throughout the world. Platelet activation and aggregation play a central role in hemostasis and thrombosis. Herbal medicines have been traditionally used in the management of cardiovascular disease and can help in modifying its progression, particul...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Keihanian F,Saeidinia A,Bagheri RK,Johnston TP,Sahebkar A

    更新日期:2018-06-01 00:00:00

  • Evidence for independent metabolism and cell surface localization of cell surface localization of cellular proteoglycans and glycosaminoglycan free chains.

    abstract::The synthesis and turnover of metabolically labeled proteoglycans from medium, cell layer, and substratum-associated compartments were characterized in four cell lines of fibroblastic origin, including a fibrosarcoma line, and in the murine melanoma cell type, B16.F10. Substantial differences were apparent between the...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Piepkorn M,Hovingh P,Linker A

    更新日期:1988-05-01 00:00:00

  • Role of mevalonic acid in the regulation of natural killer cell cytotoxicity.

    abstract::Considerable evidence has accumulated for a role of a nonsteroidal mevalonate product in the regulation of DNA replication and cell division. We report here a similar requirement for mevalonate in a nonreplicative function, that of natural killer (NK) cell cytotoxicity. Treatment of NK cells with 10 microM compactin f...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Cutts JL,Scallen TJ,Watson J,Bankhurst AD

    更新日期:1989-06-01 00:00:00

  • Cyclic AMP-independent processes mediate Kirsten sarcoma virus-induced changes in collagen production and other properties of cultured cells.

    abstract::Previous studies suggested that the decreased collagen production observed in Kirsten sarcoma virus (Ki-MSV)-transformed BALB 3T3 cells could be reversed by treating cells with Bt2cAMP. We examined the relationship between intracellular cAMP, collagen production, and other properties in NRK and BALB 3T3 cells transfor...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Majmudar G,Peterkofsky B

    更新日期:1985-01-01 00:00:00

  • CircHIPK3 promotes proliferation and invasion in nasopharyngeal carcinoma by abrogating miR-4288-induced ELF3 inhibition.

    abstract::Circular RNAs (circRNAs) are reported to regulate the development and progression of multiple cancers. However, the functions of circRNAs in nasopharyngeal carcinoma (NPC) are unclear. In this study, we identified that circular homeodomain interacting protein kinase 3 (circHIPK3) was highly expressed in NPC tissues an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ke Z,Xie F,Zheng C,Chen D

    更新日期:2019-02-01 00:00:00

  • Light-emitting diode irradiation induces AKT/mTOR-mediated apoptosis in human pancreatic cancer cells and xenograft mouse model.

    abstract::The beneficial effects of light-emitting diode (LED) irradiation have been reported in various pathologies, including cancer. However, its effect in pancreatic cancer cells remains unclear. Herein, we demonstrated that blue LED of 460 nm regulated pancreatic cancer cell proliferation and apoptosis by suppressing the e...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kim YM,Ko SH,Shin YI,Kim Y,Kim T,Jung J,Lee SY,Kim NG,Park KJ,Ryu JH

    更新日期:2021-02-01 00:00:00

  • Functional measurement of local proteolytic activity in living cells of invasive and non-invasive tumors.

    abstract::Proteolytic cleavage of extracellular matrix (ECM) and disruption of tissue architecture are fundamental features of tumor cell invasion. The proteolytic activity is focused in close proximity to the tumor cells. Here, we describe the possibility to quantify local proteolytic activity in the microenvironment of larger...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ludwig T,Püttmann S,Bertram H,Tatenhorst L,Paulus W,Oberleithner H,Senner V

    更新日期:2005-03-01 00:00:00

  • Disparate modulation of plasma membrane protein lateral mobility by various cell permeabilizing agents.

    abstract::The mobility of a cell surface protein on cells osmotically swollen by treatment with several different cell permeabilizing agents retains specific restraints despite detachment of the plasma membrane from the cortical cytoskeleton. Fluorescence photobleaching recovery experiments indicate that the lateral diffusion c...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Feder TJ,Chang EY,Holowka D,Webb WW

    更新日期:1994-01-01 00:00:00

  • Regrowth kinetics of cells from different regions of multicellular spheroids of four cell lines.

    abstract::A basic understanding of the recruitment of quiescent tumor cells into the cell cycle would be an important contribution to tumor biology and therapy. As a first step in pursuing this goal, we have investigated the regrowth kinetics of cells from different regions in multicellular spheroids of rodent and human origin....

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Freyer JP,Schor PL

    更新日期:1989-02-01 00:00:00

  • Ca2+ -dependent and Ca2+ -independent somatic release from trigeminal neurons.

    abstract::Besides the nerve endings, the soma of trigeminal neurons also respond to membrane depolarizations with the release of neurotransmitters and neuromodulators in the extracellular space within the ganglion, a process potentially important for the cross-communication between neighboring sensory neurons. In this study, we...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sforna L,Franciolini F,Catacuzzeno L

    更新日期:2019-07-01 00:00:00

  • Preadipocytes in the human subcutaneous adipose tissue display distinct features from the adult mesenchymal and hematopoietic stem cells.

    abstract::The stroma-vascular fraction (SVF) of human adipose tissue has recently been described to be composed of endothelial cells identified as CD34+/CD31+ cells, infiltrated/resident macrophages defined as CD14+/CD31+ cells, and a new cell population characterized as CD34+/CD31- cells. To elucidate the cell identity of the ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Sengenès C,Lolmède K,Zakaroff-Girard A,Busse R,Bouloumié A

    更新日期:2005-10-01 00:00:00

  • The potential role of senescence in limiting fibrosis caused by aging.

    abstract::Fibrosis-related diseases carry with them a high mortality rate and their morbidity increases with age. Recent findings indicate that induced senescence in myofibroblasts can limit or reduce myocardial fibrosis, cirrhosis, and idiopathic pulmonary fibrosis, while also accelerating wound healing. However, more senescen...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Meng X,Wang H,Song X,Clifton AC,Xiao J

    更新日期:2020-05-01 00:00:00

  • Release of potassium, lipids, and proteins from nonionic detergent treated chicken red blood cells.

    abstract::The plasma membrane of erythrocytes, as of other cells, is thought to act as the barrier responsible for maintaining intracellular gradients of most ions and small molecular species between the cell and its environment. Controlled application of the nonionic detergent Brij 58 effectively opened the erythrocyte plasma ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kellermayer M,Ludány A,Miseta A,Kŏszegi T,Berta G,Bogner P,Hazlewood CF,Cameron IL,Wheatley DN

    更新日期:1994-05-01 00:00:00

  • Biphasic effects of 17-beta-estradiol on expression of occludin and transendothelial resistance and paracellular permeability in human vascular endothelial cells.

    abstract::Tight junctions govern the paracellular permeability of endothelial and epithelial cells. Aberrations of tight junction function are an early and key event during the vascular spread of cancer and inflammation. This study sought to determine the role of estrogen in the regulation of tight junctions and expression of m...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ye L,Martin TA,Parr C,Harrison GM,Mansel RE,Jiang WG

    更新日期:2003-08-01 00:00:00

  • Oxidative stress and increased eNOS and NADPH oxidase expression in mouse microvessel endothelial cells.

    abstract::Elevated oxidative stress plays a key role in diabetes-associated vascular disease. In this study, we tested the hypothesis that high glucose-induced oxidative stress was associated with changes in the expression of NADPH oxidase, superoxide dismutase (SOD) and endothelial nitric oxide synthase (eNOS). Oxidative stres...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ding H,Aljofan M,Triggle CR

    更新日期:2007-09-01 00:00:00

  • Inhibition of Na+, K+-ATPase activates swelling-induced taurine efflux in a human neuroblastoma cell line.

    abstract::The Na+ pump (Na+, K+-ATPase) has been implicated in the regulation of many cellular functions, including cell volume regulation. The effects of inhibiting Na+ pump activity on cell volume and taurine efflux were evaluated in the human neuroblastoma cell line CHP-100. Cell volume changes monitored with the Coulter Mul...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Basavappa S,Mobasheri A,Errington R,Huang CC,Al-Adawi S,Ellory JC

    更新日期:1998-02-01 00:00:00

  • Intracellular localization of ClC chloride channels and their ability to form hetero-oligomers.

    abstract::ClC chloride channels (ClCs) can be classified into two groups in terms of their cellular localizations: ClCs present in the plasma membranes and those residing in intracellular organelles. Members of the latter group, including ClC-3, ClC-4, ClC-5, ClC-6, and ClC-7, are often co-expressed in a variety of cell types i...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Suzuki T,Rai T,Hayama A,Sohara E,Suda S,Itoh T,Sasaki S,Uchida S

    更新日期:2006-03-01 00:00:00

  • An Overview on Predictive Biomarkers of Testicular Germ Cell Tumors.

    abstract::Testicular germ cell tumors (TGCTs) are frequent solid malignant tumors and cause of death in men between 20-40 years of age. Genetic and environmental factors play an important role in the origin and development of TGCTs. Although the majority of TGCTs are responsive to chemotherapy, about 20% of patient presents inc...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Chieffi P

    更新日期:2017-02-01 00:00:00

  • Methylation-independent CHFR expression is a potential biomarker affecting prognosis in acute myeloid leukemia.

    abstract::CHFR acts as a tumor suppressor gene, which is frequently inactivated caused by its promoter hypermethylation in various solid tumors. Although a recent study showed that CHFR hypermethylation was a frequent event in acute myeloid leukemia (AML) and correlated with adverse clinical outcome, herein, we found that CHFR ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhou JD,Zhang TJ,Li XX,Ma JC,Guo H,Wen XM,Yao DM,Zhang W,Lin J,Qian J

    更新日期:2018-06-01 00:00:00

  • Angiotensin type 1 receptor autoantibody from preeclamptic patients induces human fetoplacental vasoconstriction.

    abstract::Increased vascular resistance in the fetoplacental circulation is a characteristic of preeclampsia. However, the potential molecular mechanisms of this condition remain obscure. The current study aimed to determine the direct effect of the peptide antigen corresponding to the second extracellular loop of the angiotens...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhang S,Zheng R,Yang L,Zhang X,Zuo L,Yang X,Bai K,Song L,Tian J,Yang J,Liu H

    更新日期:2013-01-01 00:00:00

  • Inhibition of BALB/c-3T3 cells in late G1: commitment to DNA synthesis controlled by somatomedin C.

    abstract::Methylglyoxal bis-(guanylhydrazone) (mGBG) blocked the stimulation of DNA synthesis in quiescent, density-inhibited BALB/c-3T3 cells treated with platelet-derived growth factor (PDGF) and platelet-poor plasma (PPP). Competence formation produced by a transient exposure to PDGF was not effected by mGBG. In contrast, mG...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wharton W,Van Wyk JJ,Pledger WJ

    更新日期:1981-04-01 00:00:00