Abstract:
:We studied the influence of hydrocortisone (HC) on hyaluronan (HA) metabolism in explants of human skin, a model retaining normal three-dimensional architecture of dermal connective tissue and dynamic growth and stratification of epidermal keratinocytes. The synthesis of hyaluronan and proteoglycans (PGs), and DNA, were determined with 3H-glucosamine and 3H-thymidine labelings, respectively. The total content and histological distribution of hyaluronan was studied utilizing a biotinylated aggrecan-link protein complex. A low concentration of HC (10(-9) M) stimulated the incorporation of 3H-glucosamine into hyaluronan in epidermis by 23% and reduced the disappearance rate of hyaluronan by 25% in chase experiments, resulting in a 74% increase in total hyaluronan (per epidermal dry weight) after a 5-day culture in 10(-9) M HC. On the other hand, a high concentration of HC (10(-5) M) reduced both synthesis (-42%) and degradation (-46%) of epidermal hyaluronan during 24 h labeling and chase periods. The cumulative effect of a 5-day treatment was a 24% decrease of total epidermal hyaluronan. The high dose (10(-5) M) also reduced keratinocyte DNA synthesis and epidermal thickness. In dermis, only the high (10(-5) M) concentration of HC was effective, inhibiting the incorporation of 3H-glucosamine into hyaluronan by 28%. No significant influences on total hyaluronan content or the disappearance rate of hyaluronan in dermal tissue was found. All HC concentrations lacked significant effects on newly synthesized PGs in epidermal and dermal tissues, but reduced the labeled PGs diffusing into culture medium. A low physiological concentration of HC thus maintains active synthesis and high concentration of hyaluronan in epidermal tissue, while high pharmacological doses of HC slows hyaluronan turnover and reduces its content in epidermis, an effect correlated with enhanced terminal differentiation, reduced proliferation rate and reduced number of vital keratinocyte layers.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Agren UM,Tammi M,Tammi Rdoi
10.1002/jcp.1041640204subject
Has Abstractpub_date
1995-08-01 00:00:00pages
240-8issue
2eissn
0021-9541issn
1097-4652journal_volume
164pub_type
杂志文章abstract::Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that is transiently expressed during the normal development of T and B lymphocytes. Phorbol 12-myristate 13-acetate (PMA) has been reported to induce maturation-like changes, including the loss of TdT, in many leukemic cell lines. We ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041420311
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abstract::The caspase recruitment domain (CARD)-containing proteins CARMA1-3 share high degree of sequence, structure and functional homology. Whereas CARMA1 and CARMA3 have been identified as crucial components of signal transduction pathways that lead to activation of NF-κB transcription factor, little is known about the func...
journal_title:Journal of cellular physiology
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abstract::The Na+ pump (Na+, K+-ATPase) has been implicated in the regulation of many cellular functions, including cell volume regulation. The effects of inhibiting Na+ pump activity on cell volume and taurine efflux were evaluated in the human neuroblastoma cell line CHP-100. Cell volume changes monitored with the Coulter Mul...
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更新日期:1998-02-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:2016-04-01 00:00:00
abstract::Ischemic stroke (IS), which is characterized by high morbidity, disability, and mortality, is recognized as a major cerebrovascular disease. MicroRNA-31 (miR-31) was reported to participate in the progression of brain disease. The present study was conducted in order to investigate the effect of miR-31 on oxidative st...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,收录出版
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更新日期:2020-03-01 00:00:00
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journal_title:Journal of cellular physiology
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abstract::The existence of cancer stem cells is debatable in numerous solid tumors, yet in leukemia, there is compelling evidence of this cell population. Leukemic stem cells (LSCs) are altered cells in which accumulating genetic and/or epigenetic alterations occur, resulting in the transition between the normal, preleukemic, a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
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更新日期:2020-06-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:1997-03-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041110311
更新日期:1982-06-01 00:00:00
abstract::Sterol regulatory element binding protein 1 (SREBP-1) is well-known as the master regulator of lipogenesis in rodents. Acyl-CoA synthetase short-chain family member 2 (ACSS2) plays a key role in lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis. ATP citrate lyase (ACLY) catalyzes the conversion of ci...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25954
更新日期:2018-02-01 00:00:00
abstract::Past work has shown that delta-opioid receptor (DOR) activation by [D-Ala(2),D-Leu(5)]-enkephalin (DADLE) attenuated the disruption of K(+) homeostasis induced by hypoxia or oxygen-glucose deprivation (OGD) in the cortex, while naltrindole, a DOR antagonist blocked this effect, suggesting that DOR activity stabilizes ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21000
更新日期:2007-07-01 00:00:00
abstract::We demonstrated previously that in serum-starved MCF-7 breast cancer cell line, Ang II increased Na+/K+ATPase activity and activated the protein kinase C zeta (PKC-zeta) (Muscella et al., 2002 J Endocrinol 173:315-323; 2003 J Cell Physiol 197:61-68.). The aim of the present study was to investigate the modulation of t...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20396
更新日期:2005-11-01 00:00:00
abstract::Triple-negative breast cancer (TNBC) exhibits a higher level of glycolytic capacity and are commonly associated with an inflammatory microenvironment, but the regulatory mechanism and metabolic crosstalk between the tumor and tumor microenvironment (TME) are largely unresolved. Here, we show that glucose transporter 3...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30189
更新日期:2021-01-09 00:00:00
abstract::Cation-Cl- cotransporters (CCCs) belong to a large family of proteins that includes 9 isoforms, two of which have still not been ascribed a transport function (CCC8 and CCC9) while the others are all known to promote Cl(-)-coupled Na+ and/or K+ movement at the cell surface. The CCCs are also included in a larger famil...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21814
更新日期:2009-09-01 00:00:00
abstract::Muc4 (also called Sialomucin complex) is a heterodimeric glycoprotein complex consisting of a peripheral O-glycosylated subunit ASGP-1 (ascites sialoglycoprotein-1) tightly but non-covalently bound to an N-glycosylated transmembrane subunit ASGP-2. Muc4/SMC can act as an intramembrane ligand for ErbB2 via an EGF-like ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20200
更新日期:2005-04-01 00:00:00
abstract::Acute myeloid leukemia (AML) is the most common type of leukemia in adults. AML cells secrete angiogenic factors to remodel vasculature and acquire chemoresistance; however, antiangiogenic drugs are often ineffective in AML treatment. Cancer cell-derived exosomes can induce angiogenesis, but their role in vascular rem...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27735
更新日期:2019-07-01 00:00:00
abstract::Baculoviral IAP repeat containing 5 (BIRC5) gene encodes the important protein as survivin, a multifunctional protein, which is involved in cellular and molecular networks, progression of cell cycle, homeostasis, developmental morphogenesis, and apoptosis. The proximal BIRC5 promoter possesses specific binding sites f...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28725
更新日期:2019-11-01 00:00:00
abstract::We have previously shown that extracellular ATP acts as a mitogen via protein kinase C (PKC)-dependent and independent pathways (Wang, D., Huang, N., Gonzalez, F.A., and Heppel, L.A. Multiple signal transduction pathways lead to extracellular ATP-stimulated mitogenesis in mammalian cells. I. Involvement of protein kin...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2021-01-25 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:2019-11-01 00:00:00
abstract::Hyperammonaemia and hyperethanolaemia are thought to be driving factors behind skeletal muscle myopathy in liver disease, that is, cirrhosis. Despite this, the singular and combined impacts of ethanol- and ammonia-induced protein catabolism are poorly defined. As such, we aimed to dissect out the effects of ammonia an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26881
更新日期:2018-12-01 00:00:00
abstract::Gastric cancer prognoses are persistently poor due to cancer's penchant to metastasize. As a crucial regulator of signal transducer and activator of transcription (STAT3) signaling, sirtuin 1's (SIRT1) function in gastric cancer has not been well understood. Here, we report upregulated expression of SIRT1 in tissues i...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
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更新日期:2019-09-01 00:00:00
abstract::The effect of the glucocorticoids, insulin, and glucose concentration on glycogen deposition in adult rat liver parenchymal cells maintained in a chemically defined, serum-free medium has been studied. Increasing the medium concentration of glucose from 5.6 mM to 30.6 mM in the absence of hormones increased cellular g...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041100313
更新日期:1982-03-01 00:00:00
abstract::Rb2/p130 tumor suppressor protein regulates cell cycle progression primarily through interactions with members of the E2F family of transcription factors and repression of the transactivation of E2F target genes. In ATRA sensitive ovarian carcinoma CA-OV3 cells, a dramatic increase in Rb2/p130 protein mediates growth ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21477
更新日期:2008-10-01 00:00:00
abstract::Dose-response curves for the inhibition of heterogeneous nuclear RNA (hnRNA) synthesis in HeLa cells by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB; 5-100 microM; 30 min) are biphasic and indicate the existence of two subpopulations of hnRNA molecules, one highly sensitive and the other completely resistant,...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041160106
更新日期:1983-07-01 00:00:00
abstract::A human osteosarcoma cell line, U-2 OS, cultured under serum-free conditions, was shown to produce a growth factor (osteosarcoma-derived growth factor, ODGF) for human-cultured glial cells, fibroblasts, and other cells. ODGF, collected from the spent medium of 2 OS cultures, was purified by a sequence involving hepari...
journal_title:Journal of cellular physiology
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更新日期:1980-11-01 00:00:00
abstract::The p53 protein directly regulates the expression of the WAF1 (wild-type p53-activated fragment 1) protein which is a cyclin-dependent kinase inhibitor (CDK1). DNA damaging agents such as ionizing or UV radiation, and some chemical agents induce WAF1 in wild-type p53 containing cells, thereby halting cell cycle progre...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199603)166:3<568::AID-JCP1
更新日期:1996-03-01 00:00:00
abstract:BACKGROUND:Pulmonary arterial hypertension (PAH) is characterized by the apoptosis resistance and hyperproliferation of pulmonary artery smooth muscle cells (PASMCs). Its pathogenesis has not been revealed. Here, we carried out experiments to investigate the functions of miR-140-5p and tumor necrosis factor-α (TNF-α). ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27642
更新日期:2019-06-01 00:00:00
abstract::Bladder cancer (BCa) is one of the most prevalent cancers of the urinary system worldwide. Accumulating evidence suggests that long noncoding RNAs (lncRNAs) perform a vital function in the pathogenesis and progression of BCa. In the current study, we identified a novel lncRNA OXCT1-AS1 and investigated its role and po...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28037
更新日期:2019-08-01 00:00:00