Abstract：
:The non-POU-domain-containing octamer binding protein (NONO; also known as p54nrb) has various nuclear functions ranging from transcription, RNA splicing, DNA synthesis and repair. Although tyrosine phosphorylation has been proposed to account for the multi-functional properties of p54nrb, direct evidence on p54nrb as a phosphotyrosine protein remains unclear. To investigate the tyrosine phosphorylation status of p54nrb, we performed site-directed mutagenesis on the five tyrosine residues of p54nrb, replacing the tyrosine residues with phenylalanine or alanine, and immunoblotted for tyrosine phosphorylation. We then preceded with luciferase reporter assays, RNA splicing minigene assays, co-immunoprecipitation, and confocal microscopy to study the function of p54nrb tyrosine residues on transcription, RNA splicing, protein-protein interaction, and cellular localization. We found that p54nrb was not phosphorylated at tyrosine residues. Rather, it has non-specific binding affinity to anti-phosphotyrosine antibodies. However, replacement of tyrosine with phenylalanine altered p54nrb activities in transcription co-repression and RNA splicing in gene context-dependent fashions by means of differential regulation of p54nrb protein association with its interacting partners and co-regulators of transcription and splicing. These results demonstrate that tyrosine residues, regardless of phosphorylation status, are important for p54nrb function. J. Cell. Physiol. 232: 852-861, 2017. © 2016 Wiley Periodicals, Inc.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Lee AR,Hung W,Xie N,Liu L,He L,Dong Xdoi
10.1002/jcp.25493subject
Has Abstractpub_date
2017-04-01 00:00:00pages
852-861issue
4eissn
0021-9541issn
1097-4652journal_volume
232pub_type
杂志文章abstract：:The effects of exposure of cultured P388D1 cells to H2O2 on intracellular free calcium ([Ca++]i) was investigated utilizing the intracellular fluorescent calcium chelator "Quin 2." [Ca++]i rose from approximately 150 nM to greater than 2 microM over a time course that was strongly dependent on the concentration of H2O...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041290314
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abstract：:Several agents that act through G-protein-coupled receptors and also stimulate phosphoinositide-specific phospholipase C (PI-PLC), including angiotensin II, vasopressin, norepinephrine, and prostaglandin (PG) F2alpha, activated the ERK1 (p44mapk) and ERK2 (p42mapk) members of the mitogen-activated protein (MAP) kinase...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199806)175:3<348::AID-JCP1
更新日期：1998-06-01 00:00:00
abstract：:Diabetic nephropathy (DN) is a kind of microvascular complications of diabetes. Long noncoding RNAs (lnRNAs) can participate in the development of various diseases, including DN. However, the function of lncRNA NEAT1 is unclear. In our present study, we reported that NEAT1 was significantly increased in streptozotocin...
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doi：10.1002/jcp.27959
更新日期：2019-08-01 00:00:00
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journal_title：Journal of cellular physiology
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doi：10.1002/jcp.29904
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doi：10.1002/jcp.20543
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doi：10.1002/(SICI)1097-4652(199811)177:2<334::AID-JCP1
更新日期：1998-11-01 00:00:00
abstract：:Genomic imprinting in gametogenesis marks a subset of mammalian genes for parent-of-origin-dependent monoallelic expression in the offspring. Embryological and classical genetic experiments in mice that uncovered the existence of genomic imprinting nearly two decades ago produced abnormalities of growth or behavior, w...
journal_title：Journal of cellular physiology
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更新日期：2002-09-01 00:00:00
abstract：:The basis of the well-known decline in cell proliferation with increasing passage number of human diploid fibroblast-like cell cultures is not known. It has been found that DNA synthesis was deficient in the remaining but still proliferating cells, but when appropriate corrections reflecting the remaining dividing cel...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041430107
更新日期：1990-04-01 00:00:00
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doi：10.1002/jcp.1041080103
更新日期：1981-07-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199808)176:2<332::AID-JCP1
更新日期：1998-08-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27499
更新日期：2019-05-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20107
更新日期：2005-01-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199905)179:2<208::AID-JCP1
更新日期：1999-05-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040920115
更新日期：1977-07-01 00:00:00
abstract：:Pyroptosis is a form of necrotic and inflammatory programmed cell death, which could be characterized by cell swelling, pore formation on plasma membranes, and release of proinflammatory cytokines (IL-1β and IL-18). The process of pyroptosis presents as dual effects: protecting multicellular organisms from microbial i...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.29268
更新日期：2020-04-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041250322
更新日期：1985-12-01 00:00:00
abstract：:Interleukin-1 is a proinflammatory and immunomodulatory cytokine that plays a crucial role in inflammatory diseases of the skin, including bacterial infections, bullous diseases, UV damage, and especially psoriasis. To characterize the molecular effects of IL-1 in epidermis, we defined the transcriptional changes in h...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21300
更新日期：2008-01-01 00:00:00
abstract：:Programmed cell death in mammary tissue was studied during natural weaning in lactating mice and after litter removal or milk stasis. All treatments stimulated mammary apoptosis, indicating that this process is an integral part of the tissue's involution after lactation. Induction of apoptosis was slower in natural we...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199609)168:3<559::AID-JCP8
更新日期：1996-09-01 00:00:00
abstract：:Cultivation of human parotid glands in serum-free medium (Ca(2+) concentration, 0.2 mM) with growth supplements resulted in isolation of a homogeneous population of epithelial cells without any mesenchymal cells. The isolated cells showed an undifferentiated phenotype with scant cytoplasmic organelles, and low levels ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10149
更新日期：2002-10-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28667
更新日期：2019-11-01 00:00:00
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journal_title：Journal of cellular physiology
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更新日期：2020-12-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041650213
更新日期：1995-11-01 00:00:00
abstract：:MicroRNA 520c and 373 (miR-520c and miR-373) have been characterized as oncogenes and play critical roles in cancer cell metastasis. However, the relationship between these two microRNAs and matrix metalloproteinases (MMPs), which are important in cancer cell metastasis, remains unknown. Here, we report new evidence i...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22993
更新日期：2012-02-01 00:00:00
abstract：:The development of the malaria parasite Plasmodium falciparum in human red blood cells induces parasite-dependent perturbations in the permselectivity properties of the host cell membrane. The changes appear as parasites develop from ring to the trophozoite stage and persist during schizogony. In the present work we a...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041140215
更新日期：1983-02-01 00:00:00
abstract：:It was the goal of this study to determine whether during long-term quiescence WI-38 cells gradually lose labile components which then need to be resynthesized before a stimulated cell can progress through G-1 and enter S. The metabolic and molecular status of WI-38 cells was systematically analyzed as they entered an...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041420117
更新日期：1990-01-01 00:00:00
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journal_title：Journal of cellular physiology
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doi：10.1002/jcp.28050
更新日期：2019-08-01 00:00:00
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journal_title：Journal of cellular physiology
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doi：10.1002/jcp.1041600315
更新日期：1994-09-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：1988-04-01 00:00:00
abstract：:DH23A cells, an alpha-difluoromethylornithine (DFMO)-resistant variant of rat hepatoma tissue culture cells (HTC), contain high levels of very stable ornithine decarboxylase (ODC). In the absence of DFMO, the high ODC activity results in a large accumulation of endogenous putrescine. Concomitant with the putrescine in...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041580205
更新日期：1994-02-01 00:00:00
abstract：:Pterostilbene (PTS), a naturally occurring stilbene, confers protection against oxidative and cytokine stress induced pancreatic β-cell apoptosis in vitro and in vivo. To provide insights into the molecular mechanism, we performed a proteomic study on the pancreas of PTS-treated diabetic mice using electrospray ioniza...
journal_title：Journal of cellular physiology
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更新日期：2019-03-01 00:00:00
