Abstract：
:Cultivation of human parotid glands in serum-free medium (Ca(2+) concentration, 0.2 mM) with growth supplements resulted in isolation of a homogeneous population of epithelial cells without any mesenchymal cells. The isolated cells showed an undifferentiated phenotype with scant cytoplasmic organelles, and low levels of alpha-amylase expression. The cells remained viable and undifferentiated for up to 24 passages when subcultured at 80% confluence in 0.2 mM Ca(2+) medium with a 1:3 split ratios. There was little cell-cell contact. A Ca(2+) switch from 0.2 to 1 mM induced cell-cell contact with translocation of desmosomal proteins from the cytoplasm to the cell membrane, and sequential differentiation of serous acinar cells with a glandular arrangement, well-developed cytoplasmic organelles and an increased level of alpha-amylase expression. These morphological changes and desmosome assembly were blocked by treatment with non-specific PKC inhibitor. Moreover, the addition of PKC activator, tetradecanoylphorbol 13-acetate (TPA), to 0.2 mM Ca(2+) medium caused transient assembly of desmosome-like structure, but did not induce cell-cell contact or morphological differentiation. Cultivation of the cells in 1.5 mM Ca(2+) medium resulted in increased stratification of the cells and reduced alpha-amylase expression. These findings provide the first demonstration that continuous cultivation in 1.0 mM Ca(2+) medium is required for cellular differentiation of salivary gland acinar cells, and maintenance of the differentiated state.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Hiraki A,Shirasuna K,Ikari T,Shinohara M,Garrod DRdoi
10.1002/jcp.10149keywords:
subject
Has Abstractpub_date
2002-10-01 00:00:00pages
55-63issue
1eissn
0021-9541issn
1097-4652journal_volume
193pub_type
杂志文章abstract：:This study investigated whether brain-derived neurotrophic factor (BDNF) regulates the L-glutamate/L-aspartate transporter (GLAST) and glutamine synthetase (GS) in mouse retinal Müller cells (RMCs) under normal and hypoxic conditions. Mouse RMCs were treated with recombinant human BDNF (50, 75, 100, 125, or 150 ng/ml...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22762
更新日期：2012-02-01 00:00:00
abstract：:Dental pulp stem cells (DPSCs) are clonogenic, self-renewing, and multi-potential DPSCs capable of differentiating into osteoblasts. In this study, primary cell cultures were obtained from human dental pulp tissue of developing third molars, and flow cytometry was used to sort the subpopulation of DPSCs with STRO-1 an...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22206
更新日期：2010-10-01 00:00:00
abstract：:Pleiotropic human KB cell mutants, selected for resistance to a conjugate of epidermal growth factor with Pseudomonas exotoxin (PE-EGF), were characterized genetically. These mutants have a pleiotropic phenotype, which includes reduced number of EGF receptors and reduced growth rate. Hybrid cells between HeLa D98 and ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041350323
更新日期：1988-06-01 00:00:00
abstract：:We previously revealed that epithelial-to-mesenchymal transition (EMT) was mediated by ΔNp63β, a splicing variant of ΔNp63, in oral squamous cell carcinoma (OSCC). Recent studies have highlighted the involvement of microRNA (miRNA) in EMT of cancer cells, though the mechanism remains unclear. To identify miRNAs respon...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26267
更新日期：2018-10-01 00:00:00
abstract：:The hexose transport system of undifferentiated L6 rat myoblasts was investigated. 2-Deoxy-D-glucose (2-DOG) and 2-deoxy-2-fluoro-D-glucose (2FG) were used as analogues to investigate the rate-limiting step of hexose uptake into the cell. Virtually all of the 2-DOG or 2FG taken up into the cell was found to be in the ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041270113
更新日期：1986-04-01 00:00:00
abstract：:The biological activities of crocin, one of the main bioactive compounds of saffron, include anti-inflammatory, antioxidant, antidepressant, and anticancer effects. Crocin has been shown to trigger the apoptosis of gastric cancer cells, but its effect on the metastasis of gastric cancer cells remains unclear. Krüppel-...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28418
更新日期：2019-08-01 00:00:00
abstract：:The Toll and Toll-like receptor signaling pathways are evolutionarily conserved pathways that regulate innate immunity in insects and mammals. While efforts have been made to clarify the signal transduction events that occur during infection, much less is known about the components that maintain immune quiescence. Her...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24472
更新日期：2014-04-01 00:00:00
abstract：:Myocardin is a transcriptional co-activator of serum response factor (SRF) and can be degraded through ubiquitin-proteasome system. Our preliminary studies unexpectedly revealed that accumulation of myocardin in response to proteasome inhibition by MG132 or lactacystin resulted in decrease of transcriptional activity ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22519
更新日期：2011-07-01 00:00:00
abstract：:Increased neovascularization is commonly observed in hemorrhagic plaques and associated with rupture of atherosclerotic lesions. This study aims to investigate whether hemin accumulated at the site of hematoma promotes neovascularization through affecting the growth and function of endothelial progenitor cells (EPCs) ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21711
更新日期：2009-06-01 00:00:00
abstract：:Chemotherapy resistance is the major issue of choriocarcinoma. Apoptosis always is the ultimate outcome of chemotherapeutic drugs, which considered one of the reasons of resistance. We investigated the role of STAT3/NFIL3 signaling-inhibited apoptosis in chemotherapy resistance and whether Raddeanin A (RA) could be a ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26362
更新日期：2018-07-01 00:00:00
abstract：:Ovarian cancer (OC) is a fatal cancer in women, mainly due to its aggressive nature and poor survival rate. The lncRNA-miRNA-mRNA (long noncoding RNA-microRNA-messenger RNA) interaction is promising biomarkers for the improving prognosis of OC. Therefore, we explored the regulatory mechanism of WDFY3-AS2/miR-18a/RORA ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29028
更新日期：2020-02-01 00:00:00
abstract：:Priapism, a prolonged penile erection in the absence of sexual arousal, is common among patients with sickle cell disease (SCD). Hypogonadism is also common in patients with SCD. While the administration of exogenous testosterone reverses hypogonadism, it is contraceptive. We hypothesized that the stimulation of endog...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.30075
更新日期：2020-09-24 00:00:00
abstract：:Comparative studies with rat transferrin (rTf) and asialotransferrin (asialo-rTf) were performed on suspended adult rat hepatocytes with the aim of elucidating the mechanism of enhanced hepatic Fe acquisition from asialo-rTf observed previously in vivo. At low ligand concentrations (0.05-20 micrograms/ml), the cells b...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041350325
更新日期：1988-06-01 00:00:00
abstract：:Thrombin is known to evoke numerous inflammatory and proliferative responses in a wide variety of its target cells. Recent studies have demonstrated morphoregulatory and mitogenic effects of thrombin on astroglial cells (astrocytes). The present study deals with thrombin-induced activation of mitogen-activated protein...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041650223
更新日期：1995-11-01 00:00:00
abstract：:The objective of this study was to establish whether human recombinant tumor necrosis factor (TNF) can significantly stimulate the proliferation of some tumor cells. Treatment with TNF had little or no effect on the growth of human tumor cells and murine NIH/3T3 cells cultured in medium with high serum concentration. ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041340321
更新日期：1988-03-01 00:00:00
abstract：:Ephrin A1 has a role in a variety of biological events, including cell proliferation, differentiation, migration, and angiogenesis. Ephrin A1 expression is abundant in trophoblasts and endometrial cells during the implantation period; however, its intracellular activities have not yet been reported in bovine endometri...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27275
更新日期：2019-04-01 00:00:00
abstract：:Nuclear factor-κB (NF-κB), a family of master regulated dimeric transcription factors, signaling transduction pathways are active players in the cell signaling that control vital cellular processes, including cell growth, proliferation, differentiation, apoptosis, morphogenesis, angiogenesis, and immune responses. Nev...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.28249
更新日期：2019-02-11 00:00:00
abstract：:The secosteroid hormone 1alpha, 25-dihydroxyvitamin D3 (1,25D) has been shown to regulate the growth and differentiation of human prostate cancer (PCa) cells, although the precise molecular mechanisms mediating these effects have not been defined. Previous studies in our laboratory demonstrated that the antiproliferat...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20692
更新日期：2006-09-01 00:00:00
abstract：:Adiponectin, one of the adipose-derived hormone with metabolic activity, has been reported to conversely affect angiogenesis of endothelial cells in vitro. The previous study in animal models has demonstrated that adiponectin has a protective role in retinal vascular injury following pathological stimuli. However, cli...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28659
更新日期：2019-11-01 00:00:00
abstract：:The frequencies of 6-thioguanine-resistant primary clones from the kidneys and skeletal muscles of aging male cohorts of two F1 hybrid strains of Mus musculus varied from 0.59 to 10.96 X 10(-5) and did not increase as a function of donor age (up to 40 months). Resistant clones were shown to be severely deficient in th...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041210207
更新日期：1984-11-01 00:00:00
abstract：:The expression profile and functions of the brain-enriched Rab22B/Rab31 small GTPase had remained uncharacterized. Using specific antibodies against Rab22B, we found the protein to be exceptionally enriched in nestin and RC2-positive radial glia of the embryonic mouse brain. In the adult brain, Rab22B is rather specif...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21911
更新日期：2009-12-01 00:00:00
abstract：:Aberrant expressions of microRNAs have been reported to be strongly associated with the progression and prognosis of various tumors, including oral squamous cell carcinoma (OSCC). Recent studies on miRNA expression profiling have suggested that microRNA-16 (miR-16) may be dysregulated in OSCC. However, the tumorigenic...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26833
更新日期：2018-12-01 00:00:00
abstract：:Studies have shown that microRNAs (miRNAs) play a vital role in tumor progression and patients' prognosis. Therefore, we aimed to construct a miRNA model for forecasting the survival of hepatocellular carcinoma (HCC) patients. The gene expression data of 433 patients with HCC from The Cancer Genome Atlas (TCGA) and Ge...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29245
更新日期：2020-04-01 00:00:00
abstract：:The expression of parathyroid hormone-related protein (PTHrP) was studied in a range of cell cultures representative of the osteoblast lineage and in rat calvarial sections. Primary newborn rat calvarial cells, a rat preosteoblastic cell line (UMR 201), a mouse stromal cell line (ST 2), a mouse calvaria-derived osteob...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199601)166:1<94::AID-JCP11
更新日期：1996-01-01 00:00:00
abstract：:We have previously shown that mitochondrial activity is an important regulator of myoblast differentiation, partly through processes targeting myogenin expression. Here, we investigated the possible involvement of c-myc in these processes. Inhibition of mitochondrial activity by chloramphenicol abrogated the decrease ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20539
更新日期：2006-04-01 00:00:00
abstract：:Heart failure is a major cause of death throughout the world. Hyperthyroidism has been shown to induce cardiac hypertrophy, which is a contributing factor to heart failure. However, the mechanism underling effect of thyroid hormone is not completely clear. The present study investigates the role of peroxisome prolifer...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24015
更新日期：2012-09-01 00:00:00
abstract：:Fibroblastic and epithelioid clones have been isolated from the normal rat kidney line, NRK. These clones were studied for their ability to bind epidermal growth factor (EGF), susceptibility to transformation by mouse sarcoma virus (MSV), and alteration in EGF binding upon sarcoma virus transformation. The epithelioid...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040940311
更新日期：1978-03-01 00:00:00
abstract：:The role of hsp27 as an inhibitor of actin polymerization was considered in the context of the actin cytoskeleton and its relationship with focal adhesion formation. The aim of this study was to evaluate the potential effects of hsp27 on focal adhesion formation as a relevant biological consequence of actin stress fib...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199812)177:4<575::AID-JCP8
更新日期：1998-12-01 00:00:00
abstract：:Tumor cells overcome anti-tumor responses in part through immunosuppressive mechanisms. There are several immune modulatory mechanisms. Among them, adenosine is an important factor which is generated by both cancer and immune cells in tumor microenvironment to suppress anti-tumor responses. Two cell surface expressed ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.25873
更新日期：2018-03-01 00:00:00
abstract：:NEAT1 is an important tumor oncogenic gene in various tumors. Nevertheless, its involvement remains poorly studied in cervical cancer. Our study explored the functional mechanism of NEAT1 in cervical cancer. NEAT1 level in several cervical cancer cells was quantified and we found NEAT1 was greatly upregulated in vitro...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28538
更新日期：2019-08-01 00:00:00
