Abstract：
:In certain epithelial tissues, activation of transient receptor potential (TRP) vanilloid subtype 1 (TRPV1) by noxious stimuli induces pro-inflammatory cytokine release, which helps to mitigate the challenge. While the corneal epithelium elicits such responses to a variety of challenges, it remains unknown whether TRPV1 mediates pro-inflammatory cytokine secretion. Accordingly, we probed for TRPV1 expression and function in human (HCEC) and rabbit corneal epithelial cell (RCEC) lines, in their primary counterparts, and in human and mouse corneal epithelium in situ. Cell membrane and perinuclear TRPV1 expression was detected in all preparations and its identity verified by Western blot analysis. Capsaicin (CAP) (1-10 microM) increased nonselective cation channel whole cell currents (2.5-fold +/- 0.5-fold between -60 and 130 mV), resulting in calcium transients that were fully blocked by the TRPV1 antagonists capsazepine (CPZ) and ruthenium red, or removal of extracellular calcium. Another signaling event involved transient activation of global mitogen-activated protein kinase (MAPK) superfamily, which was followed by up to 3.3- and 9-fold increases in interleukins (IL)-6 and -8 release, respectively. Such increases in inflammatory mediators' release were suppressed by exposure to CPZ or MAPK inhibitors, or removal of Ca2+. Taken together, TRPV1 receptors may play a role in mediating corneal epithelial inflammatory mediator secretion and subsequent hyperalgesia.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Zhang F,Yang H,Wang Z,Mergler S,Liu H,Kawakita T,Tachado SD,Pan Z,Capó-Aponte JE,Pleyer U,Koziel H,Kao WW,Reinach PSdoi
10.1002/jcp.21141subject
Has Abstractpub_date
2007-12-01 00:00:00pages
730-9issue
3eissn
0021-9541issn
1097-4652journal_volume
213pub_type
杂志文章abstract：:Modeling breast cancer in the mouse has helped to better define the heterogeneity of human breast cancer. In the recent past, it has become evident that some limitations have restricted the potential benefits that can be achieved with this approach. In this review, we highlight some key points that should be taken int...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.24031
更新日期：2012-08-01 00:00:00
abstract：:The role of mitochondrial dysfunction and its molecular mechanism in inflammation-induced acute liver failure (ALF) remain unknown. Despite the numerous studies performed to date, very few therapies are available for inflammation-induced ALF. Therefore, our study is aimed to explore the regulatory effects of mitochond...
journal_title：Journal of cellular physiology
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更新日期：2019-11-01 00:00:00
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journal_title：Journal of cellular physiology
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doi：10.1002/jcp.1041370122
更新日期：1988-10-01 00:00:00
abstract：:Acetylcholine (ACh) protected against cardiac injury via promoting autophagy and mitochondrial biogenesis, however, the involvement of mitophagy in ACh-elicited cardioprotection remains unknown. In the present study, H9c2 cardiomyocytes were subjected to hypoxia/reoxygenation (H/R) and ACh treatment during reoxygenati...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25215
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pub_type： 杂志文章
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更新日期：1990-06-01 00:00:00
abstract：:Tobacco smoking is associated with many diseases. Addiction is of the most notorious tobacco-related syndrome and is mainly attributed to nicotine. In this study, we employed Caenorhabditis elegans as a biological model to systemically investigate the effect of chronic nicotine exposure on microRNA (miRNA) expression ...
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更新日期：2014-01-01 00:00:00
abstract：:Breast cancer (BC) is the most prevalent malignant cancer in the world, is the leading cause of cancer-related death female. Recently, there is accumulating evidence that long noncoding RNAs (lncRNAs) might as an important role in the progression of BC. (epithelial-mesenchymal transition (EMT) is considered to play a ...
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更新日期：2000-03-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041410125
更新日期：1989-10-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041460303
更新日期：1991-03-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041390311
更新日期：1989-06-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10409
更新日期：2004-02-01 00:00:00
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更新日期：2019-06-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.27990
更新日期：2019-08-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041360302
更新日期：1988-09-01 00:00:00
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journal_title：Journal of cellular physiology
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doi：10.1002/jcp.1040890451
更新日期：1976-12-01 00:00:00
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更新日期：1985-11-01 00:00:00
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journal_title：Journal of cellular physiology
pub_type： 杂志文章
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更新日期：1992-12-01 00:00:00
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journal_title：Journal of cellular physiology
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更新日期：2004-06-01 00:00:00
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更新日期：2011-08-01 00:00:00
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更新日期：2007-11-01 00:00:00
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更新日期：1983-04-01 00:00:00
abstract：:Rat 3Y1 cells arrested at early S by hydroxyurea traversed the remainder of S and G2 and completed mitosis after removal of the drug, irrespective of the absence of serum from the culture medium. When cells were deprived of serum for a period between early S and mitosis after removal of hydroxyurea, the cells delayed ...
journal_title：Journal of cellular physiology
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更新日期：1982-03-01 00:00:00
abstract：:Our previous studies have demonstrated that platelet-targeted factor IX (FIX) gene therapy can introduce sustained platelet-FIX expression in hemophilia B (FIXnull ) mice. In this study, we aimed to enhance platelet-FIX expression in FIXnull mice with O6 -methylguanine-DNA-methyltransferase (MGMT)-mediated in vivo dru...
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更新日期：2021-01-01 00:00:00
abstract：:The next-generation sequencing (NGS) based RNA sequencing (RNA-Seq) and transcriptome profiling offers an opportunity to unveil complex biological processes. Successful RNA-Seq and transcriptome profiling requires a large amount of high-quality RNA. However, NGS-quality RNA isolation is extremely difficult from recalc...
journal_title：Journal of cellular physiology
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更新日期：2018-03-01 00:00:00
abstract：:Ornithine decarboxylase (ODC) activity usually rises to a peak a few hours after a trophic stimulus. The stimulation of ODC has been shown to depend on extracellular calcium in several in vitro eukaryotic systems. We have investigated the effect of calcium concentration on ODC activity and have found that ODC is stimu...
journal_title：Journal of cellular physiology
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更新日期：1984-01-01 00:00:00
abstract：:Studies have shown that microRNAs (miRNAs) play a vital role in tumor progression and patients' prognosis. Therefore, we aimed to construct a miRNA model for forecasting the survival of hepatocellular carcinoma (HCC) patients. The gene expression data of 433 patients with HCC from The Cancer Genome Atlas (TCGA) and Ge...
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更新日期：2020-04-01 00:00:00
abstract：:Serine/threonine protein phosphatase (PP) 2A regulates many biological processes, however it remains unclear whether PP2A participates in cadherin-mediated cell-cell adhesion. We show here that the core enzyme of PP2A (PP2A-AC) is localized in the cell-cell adhesion sites between adjacent cells and associated with the...
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更新日期：2006-03-01 00:00:00
