Abstract：
:Incubation of cultured bovine pulmonary artery endothelial cells with 200 microM of 3-isobutyl-1-methylxanthine (IBMX) for 24 hr produced a five- to tenfold increase in cellular angiotensin converting enzyme activity (ACE) above that of untreated control cells. A lesser increase was observed in medium ACE. Other methylxanthines produced a similar, but less marked, effect. The elevation of ACE seemed to require de novo protein synthesis since it was reduced by 0.1 microgram/ml cycloheximide. Elevation of cellular cAMP was detected at 30 min after introduction of IBMX, then rapidly returned to control levels at 1 hour, while elevation in cellular ACE at 24 hr required contact with IBMX for at least 2 hr. Hence, the transient elevation in cAMP is unlikely to be the cause of the elevation of ACE. Phorbol ester and synthetic diacyl glycerol OAG, activators of protein kinase C, did not elevate ACE. Indomethacin, at a concentration known to inhibit cyclooxygenase activity, had no effect on the elevation of ACE. The elevation of ACE by IBMX was not affected by the calcium channel blocker verapamil or the calcium chelator EGTA. In contrast, the effect of IBMX was totally abolished by the calmodulin inhibitors trifluoperazine and calmidazolium. The data show that IBMX elevates endothelial cell ACE and suggest that the elevation is mediated by a calcium-calmodulin complex. The studies demonstrate a novel effect of methylxanthines on endothelial cells in culture.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Dasarathy Y,Fanburg BLdoi
10.1002/jcp.1041370122subject
Has Abstractpub_date
1988-10-01 00:00:00pages
179-84issue
1eissn
0021-9541issn
1097-4652journal_volume
137pub_type
杂志文章abstract：:The ultimate destiny of a cell to undergo division, differentiation, survival, and death results from an intricate balance between multiple regulators including oncogenes, tumor suppressor genes, and cell cycle associated proteins. Deregulation of the cell cycle machinery switches the phenotype from a normal cell to a...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.10117
更新日期：2002-08-01 00:00:00
abstract：:The genetic factors known to be involved in the final realization of beta-glucuronidase activity in mice are considered from the standpoint of structural genes determining the catalytic activity of enzyme molecules as well as the recognition features of enzyme molecules that identify them for subsequent processing by ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040850406
更新日期：1975-04-01 00:00:00
abstract：:This study examined the role of agonist-induced Rho kinase (ROCK) involvement in the morphological outcome of pulmonary-derived fibroblasts. Normal human lung fibroblasts (NHLF) spontaneously differentiate into network-like structures in a two-dimensional growth factor reduced Matrigel matrix-based assay. Sphingosine ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20939
更新日期：2007-05-01 00:00:00
abstract：:Tumor ischemia participates in angiogenesis and cancer progression through cellular responses to hypoxia and nutrient deprivation. However, the contribution of amino acids limitation to this process remains poorly understood. Using serum-free cell culture conditions, we tested the impact of L-glutamine deprivation on ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21044
更新日期：2007-08-01 00:00:00
abstract：:The synthesis and degradation of pectoralis myosin have been investigated in chickens 15-23 days after hatching. An essentially nonreutilizable tracer amino acid was used to demonstrate differences in the rates of synthesis and degradation of myosin isolated from normal and hypertrophied, cystrophic breast tissue. The...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040860406
更新日期：1975-10-01 00:00:00
abstract：:Damage-associated molecular patterns, including mitochondrial DNA (mtDNA) are released during hemorrhage resulting in the development of endotheliopathy. Tranexamic acid (TXA), an antifibrinolytic drug used in hemorrhaging patients, enhances their survival despite the lack of a comprehensive understanding of its cellu...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28603
更新日期：2019-11-01 00:00:00
abstract：:The aim of this study was to identify the molecules and pathways involved in the cross-talk between meniscus and synovium that may play a critical role in osteoarthritis (OA) pathophysiology. Samples of synovium and meniscus were collected from patients with early and end-stage OA and cultured alone or cocultured. Cyt...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27766
更新日期：2019-07-01 00:00:00
abstract：:With the long-range goals of elucidating the biochemical genetics and the phenotypic expression, both in vitro and in vivo, of mutator strains of mammalian cells, we have isolated and partially characterized a series of drug-resistant mutants from a subline a feeder-dependent parental mouse teratocarcinoma line (PSA-1...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041150103
更新日期：1983-04-01 00:00:00
abstract：:A mutant (A204) of Chinese hamster ovary cells (CHO-K1), which is deficient in dihydroorotate (DHO) dehydrogenase (E.C. 1.3,3.1) activity, has been isolated by a replica plating procedure. The mutant does not show a requirement for exogenously added pyrimidines. Examination of intact cells shows that the mutant accumu...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040980304
更新日期：1979-03-01 00:00:00
abstract：:Fluorescence anisotropy measurements are widely used as sensitive indicators of cell membrane fluidity. 1-[4-(trimethylamino)phenyl]-6-phenyl hexa-1,3,5-triene (TMA-DPH) is a cationic fluorescent aromatic hydrocarbon that anchors at the lipid-water interface of membrane lipid bilayers. Its uptake into porcine pulmonar...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041340114
更新日期：1988-01-01 00:00:00
abstract：:Tumor progression is characterized by loss of cell adhesion and increase of invasion and metastasis. The cell adhesion molecule E-cadherin is frequently down-regulated or mutated in tumors. In addition to down-regulation of cell adhesion, degradation of the extracellular matrix by matrix metalloproteinases is necessar...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20192
更新日期：2005-03-01 00:00:00
abstract：:Suppressor of cytokine signaling (SOCS)-2 regulates normal postnatal growth and its deficiency in mice causes gigantism with increased bone length and proportional enlargement in skeletal muscles. Using C2C12 mesenchymal precursor cell line as a model, we investigated a possible role of SOCS-2 in the differentiation p...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20579
更新日期：2006-05-01 00:00:00
abstract：:Photoperiod regulates the seasonal reproductive rhythms of mammals by influencing the development and function of sexual organs; however, the underlying mechanism remains unclear. We examined the morphology and functioning of the main sex organs of striped dwarf hamsters (Cricetulus barabensis) under different photope...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29998
更新日期：2021-03-01 00:00:00
abstract：:The transforming growth factor-beta (TGF-beta) superfamily includes more than 30 members which have a broad array of biological activities. TGF-beta superfamily ligands bind to type II and type I serine/threonine kinase receptors and transduce signals via Smad proteins. Receptor-regulated Smads (R-Smads) can be classi...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.1080
更新日期：2001-06-01 00:00:00
abstract：:The in vitro culture of porcine bone marrow-derived mesenchymal stem cells (MSCs) was used for the investigation of adult stem cell biology. Isolated porcine MSCs possessed the ability to proliferate extensively in an antioxidants-rich medium containing 5% fetal bovine serum (FBS). Greater than 40 serial MSC passages ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20376
更新日期：2005-11-01 00:00:00
abstract：:A mutant of BHK cells (ts422E) temperature-sensitive for processing 32S rRNA to 28S rRNA (Toniolo et al., '73) also loses the ability to synthesize polyamines and 5.8S rRNA when shifted to the non-permissive temperature (39 degrees). The activity of several enzymes not involved with polyamine synthesis, methylation of...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041010303
更新日期：1979-12-01 00:00:00
abstract：:We recently reported that the growth of normal rat pleural mesothelial cells (RPMCs) is inhibited by conditioned media from either in vivo or in vitro transformed RPMCs. In this study we report that the growth of normal RPMCs is inhibited by epidermal growth factor (EGF). This was demonstrated by using three methods o...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041440118
更新日期：1990-07-01 00:00:00
abstract：:We evaluated the role of CCL20 (MIP-3alpha) chemokine in cells directly involved in the remodeling of bone tissue (osteoblasts and osteoclasts) and we confirmed its expression in the subchondral bone tissue of rheumatoid arthritis (RA) patients. The expression of CCL20 and of its receptor CCR6 was evaluated in osteobl...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20905
更新日期：2007-03-01 00:00:00
abstract：:Osteoporosis can result from intestinal inflammation, as is seen with inflammatory bowel disease. Probiotics, microorganisms that provide a health benefit to the host when ingested in adequate amounts, can have anti-inflammatory properties and are currently being examined to treat inflammatory bowel disease. Here, we ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24340
更新日期：2013-08-01 00:00:00
abstract：:Cancer cells in poorly vascularized solid tumors are constantly or intermittently exposed to stressful microenvironments, including glucose deprivation, hypoxia, and other forms of nutrient starvation. These tumor-specific conditions, especially glucose deprivation, activate a signaling pathway called the unfolded pro...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22085
更新日期：2010-07-01 00:00:00
abstract：:Thrombin is known to evoke numerous inflammatory and proliferative responses in a wide variety of its target cells. Recent studies have demonstrated morphoregulatory and mitogenic effects of thrombin on astroglial cells (astrocytes). The present study deals with thrombin-induced activation of mitogen-activated protein...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041650223
更新日期：1995-11-01 00:00:00
abstract：:Proteinuria in diabetic nephropathy has been correlated with reduction in heparan sulfate proteoglycan (HSPG) content of the glomerular basement membrane. We have previously shown that the underlying mechanism probably involves reduction in the synthesis by glomerular epithelial cells. In this study we explored whethe...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199604)167:1<131::AID-JCP1
更新日期：1996-04-01 00:00:00
abstract：:This study investigated whether brain-derived neurotrophic factor (BDNF) regulates the L-glutamate/L-aspartate transporter (GLAST) and glutamine synthetase (GS) in mouse retinal Müller cells (RMCs) under normal and hypoxic conditions. Mouse RMCs were treated with recombinant human BDNF (50, 75, 100, 125, or 150 ng/ml...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22762
更新日期：2012-02-01 00:00:00
abstract：:Non-small cell lung cancer (NSCLC) remains the leading cause of cancer death worldwide. As a platinum-based chemotherapeutic drug, cisplatin has been used in the NSCLC treatment for over 30 years, and its effects are impaired by drug resistance. This study aimed to investigate the potential role of lncRNA-AC078883.3 i...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28832
更新日期：2019-12-01 00:00:00
abstract：:Chrysophanol is an anthraquinone compound, mainly isolated from rhubarb, with anti-cancer effects on some types of cancer cells. However, effects of chrysophanol on human choriocarcinoma cells are not known. Therefore, the objective of this study was to determine effects of chrysophanol on choriocarcinoma cells (JAR a...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.25423
更新日期：2017-02-01 00:00:00
abstract：:Established RAW264.7 cell lines for osteoclastic differentiation has been widely engaged in bone homeostasis research, however, the efficacy of RANKL independently stimulating has rarely been defined, because protocols were usually developed and modified by various laboratories. Otherwise, problematic issues are also ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27852
更新日期：2019-07-01 00:00:00
abstract：:The ratio of mouse to rat albumin secreted by mouse hepatoma x rat hepatoma hybrid cells is constant (of the order of 5.0) irrespective of the total amounts produced. The present results establish for seven independent hybrid clones that the coordination in the ratio of mouse to rat product applies also at the level o...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041150211
更新日期：1983-05-01 00:00:00
abstract：:Inflammatory bowel disease (IBD), as a chronic and recurrent inflammatory disorder, is caused by a dysregulated and aberrant immune response to exposed environmental factors in genetically susceptible individuals. Despite huge efforts in determining the molecular pathogenesis of IBD, an increasing worldwide incidence ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.27173
更新日期：2019-04-01 00:00:00
abstract：:Eukaryotic translation initiation factor 5A (eIF5A) has a unique character: the presence of an unusual amino acid, hypusine, which is formed by post-translational modifications. Even before the identification of hypusination in eIF5A, the correlation between hypusine formation and protein synthesis, shifting cell prol...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22229
更新日期：2010-11-01 00:00:00
abstract：:An isoform of the Na(+)/Ca(2+) exchanger (SDNCX1.10) was cloned from mesangial cells of Sprague-Dawley rat. Regulation of this isoform was compared to two other clones that were derived from the Dahl/Rapp salt sensitive (SNCX) and salt resistant rat (RNCX). All isoforms differ at the alternative splice site and at ami...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10447
更新日期：2004-05-01 00:00:00
