Abstract：
:Thrombin is known to evoke numerous inflammatory and proliferative responses in a wide variety of its target cells. Recent studies have demonstrated morphoregulatory and mitogenic effects of thrombin on astroglial cells (astrocytes). The present study deals with thrombin-induced activation of mitogen-activated protein (MAP) kinase in primary cultures of rat astrocytes. Treatment of serum-starved astrocytes with thrombin resulted in a rapid activation of tyrosine (Tyr) phosphorylation of a set of proteins including a prominent one with a molecular mass of 42 kDa (p42). The identity of p42 with MAP kinase was confirmed by MAP kinase-immunoreactivity of isolated [i.e., immunoprecipitated with anti-phosphotyrosine (PY) antibodies] p42 and by increased myelin basic protein (MBP) kinase activity present in MAP kinase immunoprecipitates of thrombin-treated cultures. Pertussis toxin (PTX) pretreatment failed to inhibit thrombin stimulation of p42 phosphorylation, indicating the lack of involvement of PTX sensitive G proteins in the mechanism of activation of MAP kinase by thrombin. Chronic exposure of cultures to phorbol 12-myristate 13-acetate to down-regulate PKC resulted in an attenuation of thrombin-induced p42 Tyr phosphorylation, although H-7, a known PKC inhibitor, failed to block thrombin effect. However, staurosporine, a nonspecific protein kinase inhibitor, prevented the activation of p42 phosphorylation. It is concluded that thrombin induces MAP kinase activation in astrocytes by a mechanism involving a staurosporine-sensitive pathway.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Bhat NR,Zhang P,Hogan ELdoi
10.1002/jcp.1041650223subject
Has Abstractpub_date
1995-11-01 00:00:00pages
417-24issue
2eissn
0021-9541issn
1097-4652journal_volume
165pub_type
杂志文章abstract：:Several hydrolase activities characteristic of the apical brush border membrane of renal proximal tubule, leucine aminopeptidase, gamma-glutamyl transpeptidase, alkaline phosphatase, maltase, and trehalase, were identified in cultures of the LLC-PK1 kidney epithelial cell line. A coordinate increase in activities of t...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041210109
更新日期：1984-10-01 00:00:00
abstract：:Vole cells transformed by avian sarcoma virus carrying the src gene lose their fibroblastic morphology, the organized cytoskeletal system of the normal fibroblastic cell, the typical fibronectin deposit around the cell membrane, and the ability to shut off multiplication when suspended in liquid medium. All of these t...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041070312
更新日期：1981-06-01 00:00:00
abstract：:Studies have shown that microRNAs (miRNAs) play a vital role in tumor progression and patients' prognosis. Therefore, we aimed to construct a miRNA model for forecasting the survival of hepatocellular carcinoma (HCC) patients. The gene expression data of 433 patients with HCC from The Cancer Genome Atlas (TCGA) and Ge...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.29245
更新日期：2020-04-01 00:00:00
abstract：:The phenotype of a ouabain-resistant Aedes albopictus cell line has been partially characterized. Treatment of ouabain-sensitive cells with 0.005-1.0 mM ouabain resulted in an 80% reduction in the uptake of 86rubidium (86Rb+), an ion with an affinity for the K+ pump binding site; ouabain-resistant cells showed only a ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041010317
更新日期：1979-12-01 00:00:00
abstract：:It has been reported that p21, p53, and p16 affect the cell cycle and cell senescence. However, their roles in keratinocyte senescence are not clear. We established primary keratinocyte strains from 15 donors and maintained them until replicative senescence; their population doublings ranged from 5.7-45.2. These strai...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4652(199904)179:1<40::AID-JCP5>
更新日期：1999-04-01 00:00:00
abstract：:Infection with Verotoxin producing Escherichia coli (VTEC) has been implicated in hemolytic uremic syndrome, the leading cause of pediatric renal failure. Verotoxin (VT) binds to globotriaosylceramide (Gal alpha 1-4Gal beta 1-4GlcCer Gb3) in susceptible cells. Gb3 is required for cytotoxicity and toxin-resistant cells...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041500324
更新日期：1992-03-01 00:00:00
abstract：:The level of poly A+ RNA in growing cultures of human diploid fibroblasts is 1.8-fold times greater than in resting cultures. The level of functional ribosomes in growing cultures is 2.8 times that in resting cultures. Since transit times are similar in both types of cells, it can be concluded that the rate of protein...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1040940212
更新日期：1978-02-01 00:00:00
abstract：:Cancer cells in poorly vascularized solid tumors are constantly or intermittently exposed to stressful microenvironments, including glucose deprivation, hypoxia, and other forms of nutrient starvation. These tumor-specific conditions, especially glucose deprivation, activate a signaling pathway called the unfolded pro...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22085
更新日期：2010-07-01 00:00:00
abstract：:This study describes the alterations induced by Interleukin-1 alpha and -beta (IL-1 alpha and IL-1 beta) on fibroblast-synthesized extracellular matrix. Fibroblasts were grown between pieces of dentin or in collagen-coated Terasaki wells for 3 or 6-9 weeks to create 3-dimensional cell-containing matrices constituted p...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041390308
更新日期：1989-06-01 00:00:00
abstract：:Diabetic nephropathy (DN) is an important factor leading to end-stage kidney disease that affects diabetes mellitus patients globally. Our previous transcriptome sequencing has identified a large group of differentially expressed long noncoding RNA (lncRNA) in early development of DN. On basis of this, we aimed to inv...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28485
更新日期：2019-08-01 00:00:00
abstract：:It has been established that syndecan-1 is an important modulator of events relevant to acute tissue repair and chronic injury responses. The current studies were designed to examine syndecan-1 expression during atherosclerotic lesion formation and whether angiotensin II influences syndecan-1 expression in macrophages...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21271
更新日期：2008-03-01 00:00:00
abstract：:In novel coronavirus disease 2019 (COVID-19), the increased frequency and overactivation of T helper (Th) 17 cells and subsequent production of large amounts of proinflammatory cytokines result in hyperinflammation and disease progression. The current study aimed to investigate the therapeutic effects of nanocurcumin ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.30233
更新日期：2020-12-28 00:00:00
abstract：:Indirect evidence for the presence of a growth inhibitor in normal human fibroblasts has been obtained previously; the inhibitory activity has been found associated with crude cell extracts, but the molecule responsible for the growth inhibition has never been isolated. We have isolated a glycopeptide fraction from hu...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041540112
更新日期：1993-01-01 00:00:00
abstract：:Wnt/β-catenin signaling pathway plays a crucial role in diabetic cardiomyopathy (DCM), thus we aimed at investigating the effect of one therapeutic approach with resveratrol (RSV) given systemically and combined treatment of RSV with mesenchymal stem cells (MSCs) that was either RSV-preconditioned or not on Wnt/β-cate...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.27947
更新日期：2019-07-01 00:00:00
abstract：:Although muscle satellite cells were identified almost 40 years ago, little is known about the induction of their proliferation and differentiation in response to physiological/pathological stimuli or to growth factors/cytokines. In order to investigate the role of the insulin-like growth factor (IGF)/IGF binding prot...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.10227
更新日期：2003-04-01 00:00:00
abstract：:17β-estradiol (E2) regulates diverse physiological effects including cell proliferation through the estrogen receptor α (ERα), which as a transcription factor drives gene transcription and as an extra-nuclear localized receptor triggers the membrane-dependent activation of diverse kinase cascades. E2 also modifies ERα...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.26251
更新日期：2018-05-01 00:00:00
abstract：:Peripheral nerve physiology and regeneration has been observed and investigated in literature but surgical applications to reconstruct and restore motor or sensory functions are still in a developmental phase. The peripheral nerve progresses slowly and incompletely compared with other tissues, it may provoke separatio...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.27299
更新日期：2019-04-01 00:00:00
abstract：:We have previously demonstrated that insulin-like growth factor binding protein-5 (IGFBP-5) is upregulated following treatment of the mouse mammary epithelial cell line HC11 with lactogenic hormones (dexamethasone, insulin, and prolactin-DIP). In addition, we have also shown that IGFBP-5 is upregulated in mammary epit...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.20587
更新日期：2006-05-01 00:00:00
abstract：:Optical indicators of the cationic, cyanine and anionic oxonol classes were used to evaluate the plasma membrane potential of animal cells in suspension and in monolayer culture. The optical signals were calibrated by using diffusion potentials either of K+ (in the presence of valinomycin) or of H+ (in the presence of...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041230306
更新日期：1985-06-01 00:00:00
abstract：:Phorbol ester-induced reorganization of the actin cytoskeleton was investigated in C6 rat glioma cells. Observations by fluorescence microscopy and photoelectron microscopy indicated that pretreatment with the transition metal chelator N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) for 1-2 h at 50 microM re...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041580216
更新日期：1994-02-01 00:00:00
abstract：:A cloned human hepatoma cell line (Li-7A), possessing epidermal growth factor (EGF) receptors numbering in the range of 10-20 pmol/10(6) cells, was inhibited in its growth by EGF as well as an antagonist monoclonal antibody (MoAb) to the EGF receptor. The mode of action of the two ligands of EGF receptors appeared to ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041340113
更新日期：1988-01-01 00:00:00
abstract：:Erythropoietin (EPO), the key hormone for erythropoiesis, also increases nitric oxide (NO) bioavailability in endothelial cells (ECs), yet the definitive mechanisms are not fully understood. Increasing evidence has demonstrated that β common receptor (βCR) plays a crucial role in EPO-mediated non-hematopoietic effects...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.22678
更新日期：2011-12-01 00:00:00
abstract：:Cation-Cl- cotransporters (CCCs) belong to a large family of proteins that includes 9 isoforms, two of which have still not been ascribed a transport function (CCC8 and CCC9) while the others are all known to promote Cl(-)-coupled Na+ and/or K+ movement at the cell surface. The CCCs are also included in a larger famil...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21814
更新日期：2009-09-01 00:00:00
abstract：:In a recent publication, Heubusch et al. (J Cell. Physiol, 122:266-272, 1985) reported changes of erythrocyte volume measured by the Coulter counter technique over a wide range of osmolalities (160 to 3000 m0sm). Their results showed a partially hindered, nonlinear response, in contrast to classical observations made ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041270314
更新日期：1986-06-01 00:00:00
abstract：:The effects of pulsatile and steady fluid flow on the mRNA levels of proto-oncogenes c-fos, c-jun, and c-myc in cultured human umbilical vein endothelial cells (HUVEC) were investigated. c-fos mRNA levels in stationary cultures were very low. A 1 Hz pulsatile flow with an average shear stress of 16 dynes/cm2 induced a...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041540118
更新日期：1993-01-01 00:00:00
abstract：:Exosomes extracted from mesenchymal stem cells (MSCs) was reported to reduce myocardial ischemia/reperfusion damage. Besides, stromal-derived factor 1 (SDF1a) functions as cardiac repair after myocardial infarction (MI). Therefore, the present study aims to identify whether exosomes (Exo) released from SDF1-overexpres...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.28070
更新日期：2019-08-01 00:00:00
abstract：:Chronic hyperglycemia and activation of receptor for advanced glycation end products (RAGE) are known risk factors for microvascular disease development in diabetic retinopathy. Thioredoxin-interacting protein (TXNIP), an endogenous inhibitor of antioxidant thioredoxin (TRX), plays a causative role in diabetes and its...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.21852
更新日期：2009-10-01 00:00:00
abstract：:In areolar "loose" connective tissue, fibroblasts remodel their cytoskeleton within minutes in response to static stretch resulting in increased cell body cross-sectional area that relaxes the tissue to a lower state of resting tension. It remains unknown whether the loosely arranged collagen matrix, characteristic of...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.24102
更新日期：2013-01-01 00:00:00
abstract：:A mutant of BHK cells (ts422E) temperature-sensitive for processing 32S rRNA to 28S rRNA (Toniolo et al., '73) also loses the ability to synthesize polyamines and 5.8S rRNA when shifted to the non-permissive temperature (39 degrees). The activity of several enzymes not involved with polyamine synthesis, methylation of...
journal_title：Journal of cellular physiology
pub_type： 杂志文章
doi：10.1002/jcp.1041010303
更新日期：1979-12-01 00:00:00
abstract：:Therapeutic application of stem cells and oncolytic viruses in cancer treatment has rapidly increased in the last decade. Oncolytic viruses are considered as a new class of anticancer agents because of their ability to selectively infect and destroy cancer cells. Furthermore, regarding the specific migratory capacity ...
journal_title：Journal of cellular physiology
pub_type： 杂志文章,评审
doi：10.1002/jcp.28320
更新日期：2019-02-15 00:00:00
