Abstract:
:In a recent publication, Heubusch et al. (J Cell. Physiol, 122:266-272, 1985) reported changes of erythrocyte volume measured by the Coulter counter technique over a wide range of osmolalities (160 to 3000 m0sm). Their results showed a partially hindered, nonlinear response, in contrast to classical observations made over more restricted osmolality ranges, using other methods. The authors suggested the underlying cause of this behavior to be a mechanical resistance of the membrane cytoskeleton. In this paper, we wish to offer a different interpretation of their results on erythrocyte osmotic behavior, based on similar experiments carried out in our laboratory, and supported by previous analyses from the literature. In particular, it is shown that the shape-factor correction to the electronic sizing measurement can correctly account for the observed deviations from linearity in the hypotonic range. In contrast, increased chemical nonideality and eventual hemolysis are the likely factors responsible for the behavior in the hypertonic range.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Massaldi HA,Richieri GV,Mel HCdoi
10.1002/jcp.1041270314subject
Has Abstractpub_date
1986-06-01 00:00:00pages
448-50issue
3eissn
0021-9541issn
1097-4652journal_volume
127pub_type
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