Stress kinase p38 mediates EGFR transactivation by hyperosmolar concentrations of sorbitol.


:Activation of the epidermal growth factor receptor (EGFR) has been shown to occur by ligand-dependent and ligand-independent mechanisms. Different molecular mechanisms have been found to be responsible for ligand-independent receptor transactivation. Here, we show that hyperosmolar concentrations of sorbitol activate the EGFR in human keratinocytes. Experiments using specific inhibitors of EGFR phosphorylation show that the increased amount of activated receptors is the result of a decreased rate of dephosphorylation. Furthermore, sorbitol treatment results in a strong activation of stress kinase p38. Treatment of the cells with SB203580, a known inhibitor of p38 alpha and beta kinases, results in impairment of receptor activation, indicating that the stress kinase is involved in receptor activation modulation. This is further reinforced by experiments showing that addition of Toxin B, known to be an inhibitor of the small Rho GTPases rac1, cdc42, and Rho A/B, to the cells results in a strong induction of EGFR activation. Our results point, therefore, to a mechanism by which osmotic shock activates EGFR through the small Rho GTPases-p38 stress kinase pathway.


J Cell Physiol


Cheng H,Kartenbeck J,Kabsch K,Mao X,Marqués M,Alonso A





Has Abstract


2002-08-01 00:00:00












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    abstract::Comparative studies with rat transferrin (rTf) and asialotransferrin (asialo-rTf) were performed on suspended adult rat hepatocytes with the aim of elucidating the mechanism of enhanced hepatic Fe acquisition from asialo-rTf observed previously in vivo. At low ligand concentrations (0.05-20 micrograms/ml), the cells b...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Rudolph JR,Regoeczi E

    更新日期:1988-06-01 00:00:00

  • α2A -AR antagonism by BRL-44408 maleate attenuates acute lung injury in rats with downregulation of ERK1/2, p38MAPK, and p65 pathway.

    abstract::Acute respiratory distress syndrome (ARDS), characterized by acute hypoxic respiratory dysfunction or failure, is a manifestation of multiple organ failure in the lung, and the most common risk factor is sepsis. We previously showed that blocking α2 -adrenoceptor (α2 -AR) could attenuate lung injury induced by endotox...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Cong Z,Li D,Tao Y,Lv X,Zhu X

    更新日期:2020-10-01 00:00:00

  • MicroRNA-1271 functions as a potential tumor suppressor in hepatitis B virus-associated hepatocellular carcinoma through the AMPK signaling pathway by binding to CCNA1.

    abstract::Hepatocellular carcinoma (HCC) is mainly associated with hepatitis B virus (HBV) infection and characterized by metastasizing and infiltrating adjacent and distant tissues. Notably, microRNA-1271 (miR-1271) is a tumor suppressor in various cancers. Therefore, we evaluate the ability of miR-1271 to influence cell proli...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chen Y,Zhao ZX,Huang F,Yuan XW,Deng L,Tang D

    更新日期:2019-04-01 00:00:00

  • Coordinate secretion of mouse alphafetoprotein, mouse albumin and rat albumin by mouse hepatoma-rat hepatoma hybrid cells.

    abstract::Mouse heptoma cells that secrete large amounts of alpha-fetoprotein (AFP) and albumin have been crossed with rat hepatoma cells that secret only albumin, and in relatively small amounts, to investigate the influence of each parental genome upon the expression of serum proteins. All of the ten independent hybrid clones...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Cassio D,Hassoux R,Dupiers M,Uriel J,Weiss MC

    更新日期:1980-09-01 00:00:00

  • In vivo growth stimulation of collagen gel embedded normal human and mouse primary mammary epithelial cells.

    abstract::A new system for studying growth of normal human mammary epithelial cells in an in vivo environment using athymic nude mice is described. Human mammary epithelial cells dissociated from reduction mammoplasty specimens were embedded within collagen gels and subsequently transplanted subcutaneously into nude mice. Histo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Popnikolov NK,Yang J,Guzman RC,Swanson SM,Thordarson G,Collins G,Talamantes F,Nandi S

    更新日期:1995-04-01 00:00:00

  • Proper E-cadherin membrane location in colon requires Dab2 and it modifies by inflammation and cancer.

    abstract::We reported that Disabled-2 (Dab2) is located at the apical membrane in suckling rat intestine. Here, we discovered that, in colon of suckling and adult mouse and of adult human, Dab2 is only at lateral crypt cell membrane and colocalized with E-cadherin. Dab2 depletion in Caco-2 cells led to E-cadherin internalizatio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Vazquez-Carretero MD,García-Miranda P,Balda MS,Matter K,Ilundáin AA,Peral MJ

    更新日期:2021-02-01 00:00:00

  • Cabbage-derived three-dimensional cellulose scaffold-induced osteogenic differentiation of stem cells.

    abstract::Herbal-derived three-dimensional scaffolds have a unique structure that represents the natural cellular microenvironment and can be potentially used for tissue engineering applications. In the present study, cabbage (Cb) leaves were decellularized and then their characteristics, such as surface roughness, wettability,...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Salehi A,Mobarhan MA,Mohammadi J,Shahsavarani H,Shokrgozar MA,Alipour A

    更新日期:2020-12-30 00:00:00

  • Pmepa1 induced by RANKL-p38 MAPK pathway has a novel role in osteoclastogenesis.

    abstract::Osteoclasts are multinucleated cells formed by fusion of preosteoclasts (POCs) derived from cells of the monocyte/macrophage lineage. We have reported a culture system that supports the formation of POCs from stroma-depleted rat bone marrow cells. Global gene expression analysis of this culture system identified genes...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Funakubo N,Xu X,Kukita T,Nakamura S,Miyamoto H,Kukita A

    更新日期:2018-04-01 00:00:00

  • Interactions of Sertoli cells with laminin are essential to maintain integrity of the cytoskeleton and barrier functions of cells in culture in the two-chambered assembly.

    abstract::The addition of anti-laminin IgG to the basal surfaces of rat Sertoli cells in culture in a two-chambered assembly results in a perturbation of F-actin arrangements, including disruption of the pericellular circumferal rings, impairments of the Sertoli cell permeability barrier, and subsequently focal defoliation, fol...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tung PS,Fritz IB

    更新日期:1993-07-01 00:00:00

  • Hypoxia Promotes the Inflammatory Response and Stemness Features in Visceral Fat Stem Cells From Obese Subjects.

    abstract::Low-grade chronic inflammation is a salient feature of obesity and many associated disorders. This condition frequently occurs in central obesity and is connected to alterations of the visceral adipose tissue (AT) microenvironment. Understanding how obesity is related to inflammation may allow the development of thera...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Petrangeli E,Coroniti G,Brini AT,de Girolamo L,Stanco D,Niada S,Silecchia G,Morgante E,Lubrano C,Russo MA,Salvatori L

    更新日期:2016-03-01 00:00:00

  • Generation of hematopoietic cells from mouse pluripotent stem cells in a 3D culture system of self-assembling peptide hydrogel.

    abstract::In vitro generation of HSCs from pluripotent stem cells (PSCs) can be regarded as novel therapeutic approaches for replacing bone marrow (BM) transplantation without immune rejection or graft versus host disease(GVHD). To date, many differentiation approaches have been evaluated in terms of directing PSCs toward diffe...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Shan W,Wang B,Xu Y,Li X,Li X,Wang H,Lin Y,Tie R,Zhao Q,Wang J,Zheng W,Hu Y,Shi J,Yu X,Huang H

    更新日期:2018-01-31 00:00:00

  • Measurements of contraction latencies to mechanical and electrical stimulation of the protozoan, Spirostomum ambiguum.

    abstract::Measurements made on contraction latencies in Spirostomun suggest that mechanical stimulation causes contractions to be initiated by the release of small amounts of calcium from a store tightly coupled to the contractile apparatus. Contraction to electrical stimulation appears to result from the gross electrophoretic ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hamilton TC,Osborn D

    更新日期:1977-06-01 00:00:00

  • Insulin restores glucose inhibition of adenosine transport by increasing the expression and activity of the equilibrative nucleoside transporter 2 in human umbilical vein endothelium.

    abstract::L-Arginine transport and nitric oxide (NO) synthesis (L-arginine/NO pathway) are stimulated by insulin, adenosine or elevated extracellular D-glucose in human umbilical vein endothelial cells (HUVEC). Adenosine uptake via the human equilibrative nucleoside transporters 1 (hENT1) and 2 (hENT2) has been proposed as a me...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Muñoz G,San Martín R,Farías M,Cea L,Vecchiola A,Casanello P,Sobrevia L

    更新日期:2006-12-01 00:00:00

  • International conference on basic and clinical aspects of cell-cycle control.

    abstract::Scientists of numerous medical and life science disciplines met in Siena, Italy to discuss the latest proceedings in basic and clinical research. General models of interconnected linear and back-feeding cell-cycle control pathways provide a basis for applied molecular research. Cell-cycle determining factors essential...

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    authors: Stiegler P,Giordano A

    更新日期:2000-12-01 00:00:00

  • In vitro paracrine regulation of human keratinocyte growth by fibroblast-derived insulin-like growth factors.

    abstract::Human keratinocytes isolated from a skin biopsy and cultured in vitro on a feeder-layer of irradiated fibroblasts reconstitute a stratified squamous epithelium suitable for grafting onto patients suffering from large burn wounds. Since conditioned medium from 3T3-J2 cells can partially substitute for the intact feeder...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Barreca A,De Luca M,Del Monte P,Bondanza S,Damonte G,Cariola G,Di Marco E,Giordano G,Cancedda R,Minuto F

    更新日期:1992-05-01 00:00:00

  • Downregulation of uPAR promotes urokinase translocation into the nucleus and epithelial to mesenchymal transition in neuroblastoma.

    abstract::The urokinase system is involved in a variety of physiological processes, such as fibrinolysis, matrix remodeling, wound healing, and regeneration. Upon binding to its cognate receptor urokinase-type plasminogen activator receptor (uPAR), urokinase-type plasminogen activator (uPA) catalyzes the conversion of plasminog...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Semina EV,Rubina KA,Shmakova AA,Rysenkova KD,Klimovich PS,Aleksanrushkina NA,Sysoeva VY,Karagyaur MN,Tkachuk VA

    更新日期:2020-09-01 00:00:00

  • PBK promotes aggressive phenotypes of cervical cancer through ERK/c-Myc signaling pathway.

    abstract::Cervical cancer is the fourth most frequent cancer in women worldwide. PDZ-binding kinase (PBK) is proven to promote the malignant behaviors of various carcinomas. However, its functional roles and oncogenic mechanisms in cervical cancer are poorly understood. In this study, we reported that PBK was highly expressed i...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ma H,Han F,Yan X,Qi G,Li Y,Li R,Yan S,Yuan C,Song K,Kong B

    更新日期:2020-11-13 00:00:00

  • The role of p63 in germ cell apoptosis in the developing testis.

    abstract::The fetal and neonatal development of male germ cells (gonocytes) is a poorly understood but crucial process for establishing fertility. In rodents, gonocytes go through two phases of proliferation accompanied by apoptosis and separated by a quiescent period during the end of fetal development. P63 is a member of the ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Petre-Lazar B,Livera G,Moreno SG,Trautmann E,Duquenne C,Hanoux V,Habert R,Coffigny H

    更新日期:2007-01-01 00:00:00

  • ADP-Induced Ca2+ Signaling and Proliferation of Rat Ventricular Myofibroblasts Depend on Phospholipase C-Linked TRP Channels Activation Within Lipid Rafts.

    abstract::Nucleotides released during heart injury affect myocardium electrophysiology and remodeling through P2 purinoceptors activation in cardiac myofibroblasts. ATP and UTP endorse [Ca2+ ]i accumulation and growth of DDR-2/α-SMA-expressing myofibroblasts from adult rat ventricles via P2Y4 and P2Y2 receptors activation, resp...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Certal M,Vinhas A,Barros-Barbosa A,Ferreirinha F,Costa MA,Correia-de-Sá P

    更新日期:2017-06-01 00:00:00

  • Myocardial proteases and cardiac remodeling.

    abstract::Cardiac hypertrophy (CH), characterized by the enlargement of cardiomyocytes, fibrosis and apoptosis, is one of the leading causes of death worldwide. Despite the advances in cardiovascular research, there remains a need to further investigate the signaling pathways that mediate CH in order to identify novel therapeut...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Riaz S,Zeidan A,Mraiche F

    更新日期:2017-12-01 00:00:00

  • CircHIPK3 promotes proliferation and invasion in nasopharyngeal carcinoma by abrogating miR-4288-induced ELF3 inhibition.

    abstract::Circular RNAs (circRNAs) are reported to regulate the development and progression of multiple cancers. However, the functions of circRNAs in nasopharyngeal carcinoma (NPC) are unclear. In this study, we identified that circular homeodomain interacting protein kinase 3 (circHIPK3) was highly expressed in NPC tissues an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ke Z,Xie F,Zheng C,Chen D

    更新日期:2019-02-01 00:00:00

  • Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation.

    abstract::Fibroblastic and epithelioid clones have been isolated from the normal rat kidney line, NRK. These clones were studied for their ability to bind epidermal growth factor (EGF), susceptibility to transformation by mouse sarcoma virus (MSV), and alteration in EGF binding upon sarcoma virus transformation. The epithelioid...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: de Larco JE,Todaro GJ

    更新日期:1978-03-01 00:00:00

  • Cellular control of ornithine decarboxylase activity by its antizyme.

    abstract::Conditions have been established under which the antizyme of ornithine decarboxylase (E.C., L-ornithine carboxy-lyase, ODC) a non-competitive protein inhibitor of ODC, can be detected in cells in response to as little as 10(-7) M putrescine. The maintenance of intracellular antizyme activity depends upon the ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Heller JS,Canellakis ES

    更新日期:1981-05-01 00:00:00

  • Intelligent TAT-coupled anti-HER2 immunoliposomes knock downed MDR1 to produce chemosensitize phenotype of multidrug resistant carcinoma.

    abstract::Gene therapy using biocompatible cationic liposomes is amongst promising approaches that decreases death from cancers. Here an invasive multidrug resistant cell model has been developed by lentiviral transfection. In parallel phospholipids have been covalently conjugated to TAT, MMP2, and Herceptin. The functional lip...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Gholamian Dehkordi N,Elahian F,Khosravian P,Mirzaei SA

    更新日期:2019-11-01 00:00:00

  • Growth stimulating activity in bovine pituitary extract specific for a rat mammary carcinoma cell line.

    abstract::We report the isolation of a bovine pituitary growth factor (MGF) for a rat mammary carcinoma cell line, 64-24, which was isolated from a highly hormone-dependent mammary tumor. The MGF has been partially purified by a series of Diaflo ultrafiltration membrane sievings, isoelectric focusing and Sephadex columns. The M...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kano-Sueoka T,Campbell GR,Gerber M

    更新日期:1977-12-01 00:00:00

  • MicroRNAs in alcoholic liver disease: Recent advances and future applications.

    abstract::Alcoholic liver disease (ALD) is characterized by hepatocyte damage, inflammatory cell activation, and increased intestinal permeability leading to the clinical manifestations of alcoholic hepatitis. Selected members of the family of microRNAs (miRNAs) are affected by alcohol, resulting in an abnormal miRNA profile in...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Xu T,Li L,Hu HQ,Meng XM,Huang C,Zhang L,Qin J,Li J

    更新日期:2018-01-01 00:00:00

  • The effect of metabolic inhibition on ion contents and sodium exchange in human lymphocytes.

    abstract::Lymphocytes depleted of ATP by incubation in iodoacetate (IAA) and nitrogen (N2) lost K and gained Na. Isotopic Na exchange showed a fast fraction and a slower exponential fraction, the latter conventionally assumed to reflect surface membrane properties. The gain of cell Na was not accounted for by a decrease in 22Na...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Negendank W,Shaller C

    更新日期:1982-03-01 00:00:00

  • Role of ecto-NTPDases on UDP-sensitive P2Y(6) receptor activation during osteogenic differentiation of primary bone marrow stromal cells from postmenopausal women.

    abstract::This study aimed at investigating the expression and function of uracil nucleotide-sensitive receptors (P2Y(2), P2Y(4), and P2Y(6)) on osteogenic differentiation of human bone marrow stromal cells (BMSCs) in culture. Bone marrow specimens were obtained from postmenopausal female patients (68 ± 5 years old, n = 18) und...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Noronha-Matos JB,Costa MA,Magalhães-Cardoso MT,Ferreirinha F,Pelletier J,Freitas R,Neves JM,Sévigny J,Correia-de-Sá P

    更新日期:2012-06-01 00:00:00

  • Topology of inositol lipid signal transduction in the nucleus.

    abstract::An increasing body of evidence shows that many of the key inositol lipids and enzymes responsible for their metabolism reside in nuclei. Moreover, the association of the nuclear phosphoinositide cycle with progression through the cell cycle and commitment toward differentiation has built a wider picture of the implica...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Maraldi NM,Zini N,Santi S,Manzoli FA

    更新日期:1999-11-01 00:00:00

  • Role of diacylglycerol (DAG) in hormonal induction of S phase in hepatocytes: the DAG-dependent protein kinase C pathway is not activated by epidermal growth factor (EGF), but is involved in mediating the enhancement of responsiveness to EGF by vasopressi

    abstract::The role of diacylglycerol (DAG) in hormonal induction of S phase was investigated in primary cultures of rat hepatocytes. In this model, several agonists that bind to G protein-coupled receptors act as comitogens when added to the cells soon after plating (i.e., in Go/early Gl phase), while the cells are most respons...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Dajani OF,Sandnes D,Melien O,Rezvani F,Nilssen LS,Thoresen GH,Christoffersen T

    更新日期:1999-08-01 00:00:00