Abstract:
:Pleiotropic human KB cell mutants, selected for resistance to a conjugate of epidermal growth factor with Pseudomonas exotoxin (PE-EGF), were characterized genetically. These mutants have a pleiotropic phenotype, which includes reduced number of EGF receptors and reduced growth rate. Hybrid cells between HeLa D98 and four out of five of these resistant cell lines were more resistant to PE-EGF than hybrids formed between HeLa D98 and parental KB cells. This result indicates that the phenotype of PE-EGF resistance is incompletely dominant in four out of five cases and recessive in one out of five variants. In three separate experiments, transfection of DNA from two of the dominant resistant cell lines resulted in transformation of wild-type KB cells to PE-EGF resistance, confirming the dominant nature of these mutations, which affect levels of EGF receptor in KB cells.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Amano F,Pastan I,Gottesman MMdoi
10.1002/jcp.1041350323subject
Has Abstractpub_date
1988-06-01 00:00:00pages
527-32issue
3eissn
0021-9541issn
1097-4652journal_volume
135pub_type
杂志文章abstract::It was shown previously that mouse bone marrow cells transformed by simian virus 40 (SV40) show a reversible cell density-dependent phenotypic transition between the nonmacrophage (rapidly growing) and the macrophage (stationary) states; cells in low-density cultures are in the growing phase, express SV40 T antigen st...
journal_title:Journal of cellular physiology
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journal_title:Journal of cellular physiology
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更新日期:2001-06-01 00:00:00
abstract::Hyperammonaemia and hyperethanolaemia are thought to be driving factors behind skeletal muscle myopathy in liver disease, that is, cirrhosis. Despite this, the singular and combined impacts of ethanol- and ammonia-induced protein catabolism are poorly defined. As such, we aimed to dissect out the effects of ammonia an...
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journal_title:Journal of cellular physiology
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更新日期:1979-03-01 00:00:00
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journal_title:Journal of cellular physiology
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